Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,869,523 G→A 6.3% L49L (CTG→CTA)  cbrA → colicin M resistance protein; FAD‑binding protein, putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,869,5230GA 6.3% 203.2 / 3.4 63L49L (CTG→CTA) cbrAcolicin M resistance protein; FAD‑binding protein, putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (32/27);  new base A (2/2);  total (34/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01

GATGGAACATTTCGACGTGGCGATTATTGGCCTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATT  >  NC_000913/3869376‑3869670
                                                                                                                                                   |                                                                                                                                                   
gATGGAACATTTCGACGTGGCGATTATTGGCCTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATTGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTctgc                                                                                                                                                    >  3:473058/1‑149 (MQ=255)
gATGGAACATTTCGACGTGGCGATTATTGGCCTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTctgc                                                                                                                                                    <  1:462171/149‑1 (MQ=255)
 aTGGAACATTTCGACGTGGCGATTATTGGCCTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTctgct                                                                                                                                                   >  3:418224/1‑149 (MQ=255)
  tGGAACATTTCGACGTGGCGATTATTGGCCTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGCGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTctgctg                                                                                                                                                  >  2:333278/1‑149 (MQ=255)
      aCATTTCGACGTGGCGATTATTGGCCTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCAc                                                                                                                                              >  3:220767/1‑149 (MQ=255)
         tttCGACGTGGCGATTATTGGCCTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCgg                                                                                                                                           >  1:280522/1‑149 (MQ=255)
              aCGTGGCGATTATTGGCCTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGAcgcg                                                                                                                                      <  2:280522/149‑1 (MQ=255)
                        tattGGCCTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCtt                                                                                                                            <  2:175858/149‑1 (MQ=255)
                              ccTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTc                                                                                                                      >  3:284016/1‑149 (MQ=255)
                              ccTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTc                                                                                                                      >  2:482327/1‑149 (MQ=255)
                              ccTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTc                                                                                                                      >  3:698161/1‑149 (MQ=255)
                                   gCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGAt                                                                                                                 <  4:473058/149‑1 (MQ=255)
                                     ccGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATgg                                                                                                               <  2:248151/149‑1 (MQ=255)
                                      cGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATggg                                                                                                              <  1:120134/149‑1 (MQ=255)
                                           ggATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGAc                                                                                                         <  1:287581/149‑1 (MQ=255)
                                                ggCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTc                                                                                                    <  1:287021/149‑1 (MQ=255)
                                                  cGTTGGCACGAAAGTTAGCAGTCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCt                                                                                                  <  1:315901/149‑1 (MQ=255)
                                                  cGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCt                                                                                                  <  3:294308/149‑1 (MQ=255)
                                                   gTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTg                                                                                                 <  4:597638/149‑1 (MQ=255)
                                                    ttGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGt                                                                                                <  2:424896/149‑1 (MQ=255)
                                                       gCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGa                                                                                             >  2:384356/1‑149 (MQ=255)
                                                        cACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGAt                                                                                            <  3:524731/149‑1 (MQ=255)
                                                         aCGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGAtg                                                                                           >  2:388080/1‑149 (MQ=255)
                                                         aCGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGAtg                                                                                           >  1:655494/1‑149 (MQ=255)
                                                         aCGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGAtg                                                                                           >  4:747551/1‑149 (MQ=255)
                                                         aCGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGAtg                                                                                           >  4:202162/1‑149 (MQ=255)
                                                         aCGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGAtg                                                                                           >  4:326810/1‑149 (MQ=255)
                                                           gAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGAtgtg                                                                                         >  4:615265/1‑149 (MQ=255)
                                                             aaGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGAt                                                                                       >  4:143797/1‑149 (MQ=255)
                                                              aGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGCCGCTTCCTGTCGATGTGATc                                                                                      <  4:165854/149‑1 (MQ=255)
                                                                ttAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGTACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGc                                                                                    <  3:498843/149‑1 (MQ=255)
                                                                ttAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGGACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGc                                                                                    <  1:150217/149‑1 (MQ=255)
                                                                   gCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTaa                                                                                 <  2:663569/149‑1 (MQ=255)
                                                                   gCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTACTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTaa                                                                                 >  4:98141/1‑149 (MQ=255)
                                                                     aGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATc                                                                               >  2:423957/1‑149 (MQ=255)
                                                                     aGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCCAATc                                                                               <  2:714112/149‑1 (MQ=255)
                                                                      ggCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCtt                                                                                                                            >  1:661880/1‑103 (MQ=255)
                                                                      ggCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATcc                                                                              >  4:516517/1‑149 (MQ=255)
                                                                      ggCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAACGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCtt                                                                                                                            <  2:661880/103‑1 (MQ=255)
                                                                       gCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCg                                                                             >  3:227527/1‑149 (MQ=255)
                                                                        cAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGc                                                                            >  1:339475/1‑149 (MQ=255)
                                                                         aaaaTGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGATGCTTCCTGTCGATGTGATCGCTAATCCGCa                                                                           >  3:744747/1‑149 (MQ=255)
                                                                             tGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGAtt                                                                       >  4:590096/1‑149 (MQ=255)
                                                                             tGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTACTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGc                                                                                    >  3:247483/1‑136 (MQ=255)
                                                                             tGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTACTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGc                                                                                    <  4:247483/136‑1 (MQ=255)
                                                                              gCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGAttt                                                                      <  3:680046/149‑1 (MQ=255)
                                                                                aGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTc                                                                    <  1:384356/149‑1 (MQ=255)
                                                                                aGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTc                                                                    <  1:485816/149‑1 (MQ=255)
                                                                                aGGTGATCGCGCTGGAT‑‑‑AAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGc                                                                 <  1:769331/149‑1 (MQ=255)
                                                                                    gATCGCGCTGGATAAAAAGAACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCg                                                                >  3:444794/1‑149 (MQ=255)
                                                                                     aTCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGt                                                               >  3:536341/1‑149 (MQ=255)
                                                                                     aTCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGt                                                               >  1:201548/1‑149 (MQ=255)
                                                                                      tCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAATCCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTc                                                              <  1:191477/149‑1 (MQ=255)
                                                                                               aTAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTc                                                     >  2:728042/1‑149 (MQ=255)
                                                                                                       cACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTcgc                                             >  3:337887/1‑149 (MQ=255)
                                                                                                                     tGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTCAcgc                               >  4:583292/1‑149 (MQ=255)
                                                                                                                       aaGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTCACGCGt                             >  2:678299/1‑149 (MQ=255)
                                                                                                                       aaGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTCACGCGt                             >  1:321846/1‑149 (MQ=255)
                                                                                                                        aGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTa                            <  4:380151/149‑1 (MQ=255)
                                                                                                                        aGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTa                            >  4:693013/1‑149 (MQ=255)
                                                                                                                         ggTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTaa                           <  3:729330/149‑1 (MQ=255)
                                                                                                                                 cAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGc                   >  3:540869/1‑149 (MQ=255)
                                                                                                                                  aaaCCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCg                  >  1:618579/1‑149 (MQ=255)
                                                                                                                                    aCCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGaa                <  4:418224/149‑1 (MQ=255)
                                                                                                                                      ccTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGc              >  2:35174/1‑149 (MQ=255)
                                                                                                                                         gcggcgGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCtat           <  3:747551/149‑1 (MQ=255)
                                                                                                                                              ggTCTACTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCaa      <  3:98141/149‑1 (MQ=255)
                                                                                                                                                  tgctgGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATAtt  >  3:187813/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GATGGAACATTTCGACGTGGCGATTATTGGCCTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATT  >  NC_000913/3869376‑3869670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑