Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,088,209 T→A 8.3% K549N (AAA→AAT frvR ← putative frv operon regulator; contains a PTS EIIA domain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,088,2090TA 8.3% 286.7 / 12.2 84K549N (AAA→AATfrvRputative frv operon regulator; contains a PTS EIIA domain
Reads supporting (aligned to +/- strand):  ref base T (43/34);  new base A (5/2);  total (48/36)
Fisher's exact test for biased strand distribution p-value = 6.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01

TTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATCGGCGGCGGCGCAGGCGATCAAGACATGGTTAATGACTTCGTTATTCACCTCAACTGGTTGGGCGAGGGTAATAAAAAATCCACGAAAACGTCGCTCCTGTTCGCTCCAGCAATGTGGGATGGCGAGG  >  NC_000913/4088061‑4088356
                                                                                                                                                    |                                                                                                                                                   
tttttGATGATCAATGCAGTGTCGGATGCGACGCCTCCGACACAGTTCACCCCTTGAGAAGTAACTCCATAAATGCCTCTTATCCTGTTAACCCGGCAAAAATCTCCCCCGGATGCTGACACAATATGCTTCCCAGATAGCTAAATAtt                                                                                                                                                     <  4:468595/149‑1 (MQ=255)
       tgatAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCt                                                                                                                                              >  2:237349/1‑149 (MQ=255)
        gatAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAAAATATTTAGCta                                                                                                                                             <  1:443423/149‑2 (MQ=255)
             aTGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGAc                                                                                                                                        >  4:729413/1‑149 (MQ=255)
               gCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGAcgc                                                                                                                                      <  2:254325/149‑1 (MQ=255)
                    gtCGGGTGCGGCGCATCCGACACAGTTCACCCCTTGTGAACTCACTCCATAAATGCCTCATATCCTGTTAACCCGTCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCAAGACGCGCATc                                                                                                                                 <  4:492681/149‑1 (MQ=255)
                     tCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATAttt                                                                                                                                                    <  2:308277/129‑1 (MQ=255)
                     tCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATAttt                                                                                                                                                    >  1:308277/1‑129 (MQ=255)
                     tCGGATGCGCCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATcg                                                                                                                                >  2:601381/1‑149 (MQ=255)
                     tCGGATGCGCCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATcg                                                                                                                                >  1:642314/1‑149 (MQ=255)
                         aTGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATcggcgg                                                                                                                            >  2:672221/1‑149 (MQ=255)
                          tGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATcggcggc                                                                                                                           <  4:755717/149‑1 (MQ=255)
                             ggcgCATCCGACACAGTTCACCCCGTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGATTGCCAGATAGCTAAATATTTTTAGATCATGACGCGCATAggcggcggc                                                                                                                        >  3:474642/1‑149 (MQ=255)
                                   tCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATCGGCGGCGGCGCAGGc                                                                                                                  <  2:627809/149‑1 (MQ=255)
                                              tCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATCGGCGGCGGCGCAGGCGATCAAGACAt                                                                                                       <  3:330762/149‑1 (MQ=255)
                                               cACCCCTTGTGAAGTAACTCCATAACTGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATCGGCGGCGGCGCAGGCGATCAAGACATg                                                                                                      >  2:2153/1‑149 (MQ=255)
                                                aCCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATCGGCGGCGGCGCAGGCGATCAAGACATgg                                                                                                     >  3:192255/1‑149 (MQ=255)
                                                     ttGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATCGGCGGCGGCGCAGGCGATCAAGACATGGTTAAt                                                                                                <  4:233617/149‑1 (MQ=255)
                                                     ttGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATCGGCGGCGGCGCAGGCGATCAAGACATGGTTAAt                                                                                                >  4:159830/1‑149 (MQ=255)
                                                           aGTAACTCCATAAATGCCTCTTATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATCGGCGGCGGCGCAGGCGATCAAGACATGGTTAATGACTTc                                                                                          >  3:50666/1‑149 (MQ=255)
                                                            gTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCAt                                                                                                                                  <  1:528753/108‑1 (MQ=255)
                                                            gTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCAt                                                                                                                                  >  2:528753/1‑108 (MQ=255)
                                                            gTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATCGGCGGCGGCGCAGGCGATCAAGACATGGTTAATGACTTCg                                                                                         >  2:643605/1‑149 (MQ=255)
                                                            gTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATCGGCGGCGGCGCAGGCGATCAAGACATGGTTAATGACTTCg                                                                                         <  2:642314/149‑1 (MQ=255)
                                                               aCTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATCGGCGGCGGCGCAGGCGATCAAGACATgg                                                                                                     >  1:461765/1‑134 (MQ=255)
                                                               aCTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATCGGCGGCGGCGCAGGCGATCAAGACATgg                                                                                                     <  2:461765/134‑1 (MQ=255)
                                                               aCTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATCGGCGGCGGCGCAGGCGATCAAGACATGGTTAATGACTTCGTTa                                                                                      <  1:725642/149‑1 (MQ=255)
                                                               aCTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATCGGCGGCGGCGCAGGCGATCAAGACATGGTTAATGACTTCGTTa                                                                                      <  1:601381/149‑1 (MQ=255)
                                                               aCTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATCGGCGGCGGCGCAGGCGATCAAGACATGGTTAATGACTTCGTTa                                                                                      <  3:25527/149‑1 (MQ=255)
                                                                    aTAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATCGGCGGCGGCGCAGGCGATCAAGACATGGTTAATGACTTc                                                                                          >  2:572674/1‑140 (MQ=255)
                                                                    aTAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATCGGCGGCGGCGCAGGCGATCAAGACATGGTTAATGACTTc                                                                                          <  1:572674/140‑1 (MQ=255)
                                                                    aTAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATCGGCGGCGGCGCAGGCGATCAAGACATGGTTAATGACTTCGTTATTCAc                                                                                 >  2:742152/1‑149 (MQ=255)
                                                                      aaaTGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATCGGCGGCGGCGCAGGCGATCAAGACATGGTTAATGACTTCGTTATTCACCt                                                                               <  4:47307/149‑1 (MQ=255)
                                                                       aaTGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATCGGCGGCGGCGCAGGCGATCAAGACATGGTTAATGACTTCGTTATTCACCTc                                                                              <  2:308582/149‑1 (MQ=255)
                                                                           ccTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATCGGCGGCGGCGCAGGCGATCAAGACATGGTTAATGACTTCGTTATTCACCTCAACt                                                                          >  1:186873/1‑149 (MQ=255)
                                                                            cTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATCGGCGGCGGCGCAGGCGATCAAGACATGGTTAATGACTTCGTTATTCACCTCAACTg                                                                         >  2:19595/1‑149 (MQ=255)
                                                                             tCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATCGGCGGCGGCGCAGGCGATCaa                                                                                                            >  1:659621/1‑113 (MQ=255)
                                                                             tCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATCGGCGGCGGCGCAGGCGATCaa                                                                                                            <  2:659621/113‑1 (MQ=255)
                                                                             tCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATCGGCGGCGGCGCAGGCGATCAAGACATGGTTAATGACTTCGTTATTCACCTCAACTgg                                                                        >  2:306536/1‑149 (MQ=255)
                                                                                tatCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATAttttt                                                                                                                                                  <  2:327923/72‑1 (MQ=255)
                                                                                tatCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATAttttt                                                                                                                                                  >  1:327923/1‑72 (MQ=255)
                                                                                tatCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATCGGCGGCGGCGCAGGCGATCAAGACATGGTTAATGACTTCGTTATTCACCTCAACTGGTTg                                                                     <  3:729413/149‑1 (MQ=255)
                                                                                    cTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATCGGCGGCGGCGCAGGCGATCAAGACATGGTTAATGACTTCGTTATTCACCTCAACTGGTTGGGCg                                                                 >  4:403933/1‑149 (MQ=255)
                                                                                    cTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAACTCATGACGCGCATCGGCGGCGGCGCAGGCGATCAAGACATGGTTAATGACTTCGTTATTCACCTCAACTGGTTGGGc                                                                  <  4:81252/148‑1 (MQ=255)
                                                                                    cTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAACTCATGACGCGCATCGGCGGCGGCGCAGGCGATCAAGACATGGTTAATGACTTCGTTATTCACCTCAACTGGTTGGGc                                                                  >  3:81252/1‑148 (MQ=255)
                                                                                     tGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATCGGCGGCGGCGCAGGCGATCAAGACATGGTTAATGACTTCGTTATTCACCTCAACTGGTTGGGCGa                                                                >  1:305510/1‑149 (MQ=255)
                                                                                     tGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAAAATATTTAGCt                                                                                                                                              <  2:235086/71‑1 (MQ=255)
                                                                                     tGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAAAATATTTAGCt                                                                                                                                              >  1:235086/1‑71 (MQ=255)
                                                                                      gTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATCGGCGGCGGCGCAGGCGATCAAGACATGGTTAATGACTTCGTTATTCACCTCAACTGGTTGGGCGAg                                                               <  1:429291/149‑1 (MQ=255)
                                                                                        tAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATCGGCGGCGGCGCAGGCGATCAAGACATGGTTAATGACTTCGTTATTCACCTCAACTGGTTGGGCGAggg                                                             <  1:743693/149‑1 (MQ=255)
                                                                                        tAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATCGGCGGCGGCGCAGGCGATCAAGACATGGTTAATGACTTCGTTATTCACCTCAACTGGTTGGGCGAggg                                                             <  3:122850/149‑1 (MQ=255)
                                                                                          aCCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATCGGCGGCGGCGCAGGCGATCAAGACATGGTTAATGACTTCGTTACTCACCTCAACTGGTTGGGCGAGGGta                                                           >  4:684723/1‑149 (MQ=255)
                                                                                           cccGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATCGGCGGCGGCGCAGGCGATCAAGACATGGTTAATGACTTCGTTATTCACCTCAACTGGTTGGGCGAGGGtaa                                                          >  1:56459/1‑149 (MQ=255)
                                                                                            ccGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATCGGCGGCGGCGCAGGCGATCAAGACATGGTTAATGACTTCGTTATTCACCTCAACTGGTTGGGCGAGGGtaat                                                         >  1:204935/1‑149 (MQ=255)
                                                                                            ccGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATCGGCGGCGGCGCAGGCGATCAAGACATGGTTAATGACTTCGTTATTCACCTCAACTGGTTGGGCGAGGGtaat                                                         >  3:99895/1‑149 (MQ=255)
                                                                                                      tctcCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATCGGCGGCGGCGCAGGCGATCAAGACATGGTTAATGACTTCGTTATTCACCTCAACTGGTTGGGCGAg                                                               <  4:395761/133‑1 (MQ=255)
                                                                                                      tctcCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATCGGCGGCGGCGCAGGCGATCAAGACATGGTTAATGACTTCGTTATTCACCTCAACTGGTTGGGCGAg                                                               >  3:395761/1‑133 (MQ=255)
                                                                                                      tctcCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATCGGCGGCGGCGCAGGCGATCAAGACATGGTTAATGACTTCGTTATTCACCTCAACTGGTTGGGCGAGGGTAATAAAAAATCCa                                               >  1:213194/1‑149 (MQ=255)
                                                                                                      tctcCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATCGGCGGCGGCGCAGGCGATCAAGACATGGTTAATGACTTCGTTATTCACCTCAACTGGTTGGGCGAGGGTAATAAAAAATCCa                                               <  4:401458/149‑1 (MQ=255)
                                                                                                        tcCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATCGGCGGCGGCGCAGGCGATCAAGACATGGTTAATGACTTCGTTATTCACCTCAACTGGTTGGGCGAGGGTAATAAAAAATCCACg                                             >  1:74540/1‑149 (MQ=255)
                                                                                                        tcCGCCGGATGAAGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATCGGCGGCGGCGAAGGCGATCAAGACATGGTTAATGACTTCGTTATTCACCTCAACTGGTTGGGCGAGGGTAATAAAAAATCCACg                                             >  2:185490/1‑149 (MQ=255)
                                                                                                         ccgccgGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATCGGCGGCGGCGCAGGCGATCAAGACATGGTTAATGACTTCGTTATTCACCTCAACTGGTTGGGCGAGGGTAATAAAAAATCCACGa                                            >  2:287325/1‑149 (MQ=255)
                                                                                                         ccgccgGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATCGGCGGCGGCGCAGGCGATCAAGACATGGTTAATGACTTCGTTATTCACCTCAACTGGTTGGGCGAGGGTAATAAAAAATCCACGa                                            >  4:412108/1‑149 (MQ=255)
                                                                                                                aTGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATCGGCGGCGGCGCAGGCGATCAAGACATGGTTAATGACTTCGTTATTCACCTCAACTGGTTGGGCGAGGGTAATAAAAAATCCACGAAAACGTc                                     <  4:50666/149‑1 (MQ=255)
                                                                                                                aTGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATCGGCGGCGGCGCAGGCGATCAAGACATGGTTAATGACTTCGTTATTCACCTCAACTGGTTGGGCGAGGGTAATAAAAAATCCACGAAAACGTc                                     <  1:237349/149‑1 (MQ=255)
                                                                                                                        acaATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATCGGCGGCGGCGCAGGCGATCAAGACATGGTTAATGACTTCGTTATTCACCTCAACTGGTTGGGCGAGGGTAATAAAAAATCCACGAAAACGTCGCTCCTGt                             >  2:255402/1‑149 (MQ=255)
                                                                                                                        acaATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATCGGCGGCGGCGCAGGCGATCAAGACATGGTTAATGACTTCGTTATTCACCTCAACTGGTTGGGCGAGGGTAATAAAAAATCCACGAAAACGTCGCTCCTGt                             >  4:437473/1‑149 (MQ=255)
                                                                                                                        acaATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATCGGCGGCGGCGCAGGCGATCAAGACATGGTTAATGACTTCGTTATTCACCTCAACTGGTTGGGCGAGGGTAATAAAAAATCCACGAAAACGTCGCTCCTGt                             >  4:262668/1‑149 (MQ=255)
                                                                                                                         caATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATCGGCGGCGGCGCAGGCGATCAAGACATGGTTAATGACTTCGTTATTCACCTCAACTGGTTGGGCGAGGGTAATAAAAAATCCACGAAAACGTCGCTCCTGtt                            <  3:1954/149‑1 (MQ=255)
                                                                                                                          aaTATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATCGGCGGCGgcgc                                                                                                                      >  3:344934/1‑58 (MQ=255)
                                                                                                                          aaTATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATCGGCGGCGgcgc                                                                                                                      <  4:344934/58‑1 (MQ=255)
                                                                                                                             atGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACTCGCATCGGCGGCGGCGCAGGCGATCAAGACATGGTTAATGACTTCGTTATTCACCTCAACTGGTTGGGCGAGGGTAATAAAAAATCCACGAAAACGTCGCTCCTGTTCGCt                        >  2:744015/1‑149 (MQ=255)
                                                                                                                             atGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATCGGCGGCGGCGCAGGCGATCAAGACATGGTTAATGACTTCGTTATTCACCTCAACTGGTTGGGCGAGGGTAATAAAAAATCCACGAAAACGTCGCTCCTGTTCGCt                        >  4:399595/1‑149 (MQ=255)
                                                                                                                                   gCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATCGGCGGCGGCGCAGGCGATCAAGACATGGTTAATGACTTCGTTATTCACCTCAACTGGTTGGGCGAGGGTAATAAAAAATCCACGAAAACGTCGCTCCTGTTCGCTCCAGCa                  >  4:519007/1‑149 (MQ=255)
                                                                                                                                      aGATAGCTAAATATTTTTAGCTCATGACGCGCATCGGCGGCGGCGCAGGCGATCAAGACATGGTTAATGACTTCGTTATTCACCTCAACTGGTTGGGCGAGGGTAATAAAAAATCCACGAAAACGTCGCTCCTGTTCGCTCCAGCAAtg               >  2:143889/1‑149 (MQ=255)
                                                                                                                                        tgaGCTAAAAATATTTAGCTCATGACGCGCATCGGCGGCGGCGCAGGCGATCAAGACATGGTTAATGACTTCGTTATTCACCTCAACTGGTTGGGCGAGGGTAATAAAAAATCCACGAAAACGTCGCTCCTGTTCGCTCCAGCAAtgtg             >  2:704377/3‑149 (MQ=255)
                                                                                                                                        tgaGCTAAAAATATTTAGCTCATGACGCGCATCGGCGGCGGCGCAGGCGATCAAGACATGGTTAATGACTTCGTTATTCACCTCAACTGGTTGGGCGAGGGTAATAAAAAATCCACGAAAACGTCGCTCCTGTTCGCTCCAGCAAtgtg             >  3:718108/3‑149 (MQ=255)
                                                                                                                                        aTAGCTAAATATTTTTAGCTCATGACGCGCATCGGCGGCGGCGCAGGCGATCAAGACATGGTTAATGACTTCGTTATTCACCTCAACTGGTTGGGCGAGGGTAATAAAAAATCCACGAAAACGTCGCTCCTGTTCGCTCCAGCAAtgtg             >  2:313075/1‑149 (MQ=255)
                                                                                                                                         tAGCTAAATATTTTTAGCTCATGACGCGCATCGGCGGCGGCGCAGGCGATCAAGACATGGTTAATGACTTCGTTATTCACCTCAACTGGTTGGGCGAGGGTAATAAAAAATCCACGAAAACGTCGCTCCTGTTCGCTCCAGCAATGTgg            <  1:713309/149‑1 (MQ=255)
                                                                                                                                         tAGCTAAATATTTTTAGCTCATGACGCGCATCGGCGGCGGCGCAGGCGATCAAGACATGGTTAATGACTTCGTTACTCACCTCAACTGGTTGGGCGAGGGTAATAAAAAATCCACGAAAACGTCGCTCCTGTTCGCTCCAGCAATGTgg            <  3:684723/149‑1 (MQ=255)
                                                                                                                                         gaGCTAAAAATATTTAGCTCATGACGCGCATCGGCGGCGGCGCAGGCGATCAAGACATGGTTAATGACTTCGTTATTCACCTCAACTGGTTGGGCGAGGGTAATAAAAAATCCACGAAAACGTCGCTCCTGTTCGCTCCAGCAATGTgg            >  1:714234/2‑149 (MQ=255)
                                                                                                                                          aGCTAAATATTTTTAGCTCATGACGCGCATCGGCGGCGGCGCAGGCGATCAAGACATGGTTAATGACTTCGTTATTCACCTCAACTGGTTGGGCGAGGGTAATAAAAAATCCACGAAAACGTCGCTCCTGTTCGCTCCAGCAATGTggg           <  2:213194/149‑1 (MQ=255)
                                                                                                                                            cTAAATATTTTTAGCTCATGACGCGCATCGGCGACGGCGCAGGCGATCAAGACATGGTTAATGACTTCGTTATTCACCTCAACTGGTTGGGCGAGGGTAATAAAAAATCCACGAAAACGTCGCTCCTGTTCGCTCCAGCAATGTGGGAt         >  1:18576/1‑149 (MQ=255)
                                                                                                                                             tAAAAATATTTAGCTCATGACGCGCATCGGCGGCGGCGCAGGCGATCAAGACATGGTTAATGACTTCGTTATTCACCTCAACTGGTTGGGCGAGGGTAATAAAAAATCCACGAAAACGTCGCTCCTGTTCGCTCCAGCAATGTGGGATg        >  1:543988/1‑149 (MQ=255)
                                                                                                                                              aaaTATTTTTAGCTCATTACGCGCATCGGCGGCGGCGCAGGCGATCAAGCCATGGTTAATGACTTCGTTATTCACCTCAACTGGTTGGGCGAGGGTAATAAAAAATCCACGAGAACGTCGCTCCTGTTCGCTCCAGCAATGTGGGATgg       <  4:474642/149‑1 (MQ=255)
                                                                                                                                                atatTTTTAGCTCATGACGCGCATCGGCGGCGGCGCAGGCGATCAAGACATGGTTAATGACTTCGTTATTCACCTCAACTGGTTGGGCGAGGGTAATAAAAAATCCACGAAAACGTCGCTCCTGTTCGCTCCAGCAATGTGGGATggcg     >  2:124432/1‑149 (MQ=255)
                                                                                                                                                 tatTTTTAGCTCATGACGCGCATCGGCGGCGGCGCAGGCGATCAAGACATGGTTAATGACTTCGTTATTCACCTCAACTGGTTGGGCGAGGGTAATAAAAAATCCACGAAAACGTCGCTCCTGTTCGCTCCAGCAATGTGGGATggcga    <  1:742152/149‑1 (MQ=255)
                                                                                                                                                  atTTTTAGCTCATGACGCGCATCGGCGGCGGCGCAGGCGATCAAGACATGGTTAATGACTTCGTTATTCACCTCAACTGGTTGGGCGAGGGTAATAAAAAATCCACGAAAACGTCGCTCCTGTTCGCTCCAGCAATGTGGGATggcgag   <  2:537775/149‑1 (MQ=255)
                                                                                                                                                  atTTTTAGCTCATGACGCGCATCGGCGGCGGCGCAGGCGATCAAGACATGGTTAATGACTTCGTTATTCACCTCAACTGGTTGGGCGAGGGTAATAAAAAATCCACGAAAACGTCGCTCCTGTTCGCTCCAGCAATGTGGGATggcgag   <  4:192255/149‑1 (MQ=255)
                                                                                                                                                   tttttAGCTCATGACGCGCATCGGCGGCGGCGCAGGCGATCAAGACATGGTTAATGACTTCGTTATTCACCTCAACTGGTTGGGCGAGGGTAATAAAAAATCCACGAAAACGTCGCTCCTGTTCGCTCCAGCAATGTGGGATggcgagg  <  1:19595/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TTTTTGATGATAAATGCAGTGTCGGATGCGGCGCATCCGACACAGTTCACCCCTTGTGAAGTAACTCCATAAATGCCTCATATCCTGTTAACCCGGCAATAATCTCCGCCGGATGCTGACACAATATGCTTGCCAGATAGCTAAATATTTTTAGCTCATGACGCGCATCGGCGGCGGCGCAGGCGATCAAGACATGGTTAATGACTTCGTTATTCACCTCAACTGGTTGGGCGAGGGTAATAAAAAATCCACGAAAACGTCGCTCCTGTTCGCTCCAGCAATGTGGGATGGCGAGG  >  NC_000913/4088061‑4088356

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: