Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,277,223 A→T 7.2% I54N (ATT→AAT)  soxS ← superoxide response regulon transcriptional activator; autoregulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,277,2230AT 7.2% 223.2 / 2.9 66I54N (ATT→AAT) soxSsuperoxide response regulon transcriptional activator; autoregulator
Reads supporting (aligned to +/- strand):  ref base A (28/33);  new base T (2/3);  total (30/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TAATCGCTGGGAGTGCGATCAAACTGCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGATAAGATCCTGAATAATT  >  NC_000913/4277076‑4277369
                                                                                                                                                   |                                                                                                                                                  
tAATCGCTGGGAGTGCGATCAAACTGCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAAt                                                                                                                                                   <  3:707974/149‑1 (MQ=255)
tAATCGCTGGGAGTGCGATCAAACTGCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAAt                                                                                                                                                   <  1:202960/149‑1 (MQ=255)
     gCTGGGAGTGCGATCAAACTGCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAAt                                                                                                                                                   <  2:297849/144‑1 (MQ=255)
     gCTGGGAGTGCGATCAAACTGCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAAt                                                                                                                                                   >  1:297849/1‑144 (MQ=255)
            gTGCGATCAAACTGCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGATTACATTCGCCaac                                                                                                                                       <  4:310817/149‑2 (MQ=255)
                gATCAAACTGCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTc                                                                                                                                   <  1:562860/149‑1 (MQ=255)
                gATCAAACTGCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTc                                                                                                                                   >  1:41050/1‑149 (MQ=255)
                  tCAAACTGCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCaa                                                                                                                                        >  2:179/1‑142 (MQ=255)
                  tCAAACTGCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCaa                                                                                                                                        <  1:179/142‑1 (MQ=255)
                    aaaCTGCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACGTAACCCAGGTCCATTGCGATATCAAAAATCGGATGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGAt                                                                                                                               <  2:478229/149‑1 (MQ=255)
                           cGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCAc                                                                                                                        <  4:422172/149‑1 (MQ=255)
                            gACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCAcc                                                                                                                       >  1:272179/1‑149 (MQ=255)
                                  aaaCGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCgg                                                                                                                 >  3:458835/1‑149 (MQ=255)
                                           agaAGGTCTGCTGCGAGACATAACCTAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGt                                                                                                        <  3:495957/149‑1 (MQ=255)
                                           agaAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGt                                                                                                        <  2:584663/149‑1 (MQ=255)
                                            gaAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGtt                                                                                                       <  4:50483/149‑1 (MQ=255)
                                                gTCTGCTGTGAAACATAACCCAGGTCCATTTCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAATAACAGGCGGCGTTGGCGATTGTAAACACCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCaa                                                                                                   <  4:467880/149‑1 (MQ=255)
                                                gTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCaa                                                                                                   <  3:307296/149‑1 (MQ=255)
                                                         gagaCATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACtt                                                                                          >  4:690500/1‑149 (MQ=255)
                                                         gagaCATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACtt                                                                                          >  1:147597/1‑149 (MQ=255)
                                                             cATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGaa                                                                                      <  3:54075/149‑1 (MQ=255)
                                                                  cccAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGcc                                                                                 <  1:435801/149‑1 (MQ=255)
                                                                       gTCCATTGCGGTATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGAtt                                                                            >  2:579536/1‑149 (MQ=255)
                                                                           aTTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGAtttttt                                                                        >  2:182057/1‑149 (MQ=255)
                                                                                gATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGTGGAATGTAATCGCCAACCGTCTGATGCGTCACCGTGCGGAACGTTTGTTGCCTGTACCACTTTGAATAACACGATTTTTTTGCGa                                                                   >  4:594302/1‑149 (MQ=255)
                                                                                gATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGa                                                                   <  2:374081/149‑1 (MQ=255)
                                                                                    tCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCaa                                                                                                   <  1:703236/113‑1 (MQ=255)
                                                                                    tCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCaa                                                                                                   >  2:703236/1‑113 (MQ=255)
                                                                                       aaaaTCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATc                                                            >  4:147296/1‑149 (MQ=255)
                                                                                       aaaaTCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATc                                                            >  1:453135/1‑149 (MQ=255)
                                                                                       aaaaTCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATc                                                            >  1:127954/1‑149 (MQ=255)
                                                                                         aaTCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCaa                                                          <  1:189779/149‑1 (MQ=255)
                                                                                          aTCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAAt                                                         <  1:130214/149‑1 (MQ=255)
                                                                                          aTCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAAt                                                         >  1:324116/1‑149 (MQ=255)
                                                                                                 gCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGc                                                  >  2:385409/1‑149 (MQ=255)
                                                                                                 gCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGc                                                  >  4:282944/1‑149 (MQ=255)
                                                                                                   tCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCgg                                                >  1:70413/1‑149 (MQ=255)
                                                                                                    cGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGc                                               <  1:385409/149‑1 (MQ=255)
                                                                                                       tggtgCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTgg                                            <  2:147597/149‑1 (MQ=255)
                                                                                                          tgCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCa                                         <  2:222620/149‑1 (MQ=255)
                                                                                                           gcgcAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCaa                                        <  1:182057/149‑1 (MQ=255)
                                                                                                             gcAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAata                                      >  4:465523/1‑149 (MQ=255)
                                                                                                                aCTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGc                                   <  4:85714/149‑1 (MQ=255)
                                                                                                                  tCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTc                                 >  1:575673/1‑149 (MQ=255)
                                                                                                                  tCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTc                                 >  4:384876/1‑149 (MQ=255)
                                                                                                                  tCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTc                                 <  4:38411/149‑1 (MQ=255)
                                                                                                                     aCGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAatat                                     <  3:146605/142‑1 (MQ=255)
                                                                                                                     aCGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAatat                                     >  4:146605/1‑142 (MQ=255)
                                                                                                                        gcggcCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAAt                           <  3:462899/149‑1 (MQ=255)
                                                                                                                         cggcCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATc                          <  3:402149/149‑1 (MQ=255)
                                                                                                                         cggcCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATc                          <  1:480730/149‑1 (MQ=255)
                                                                                                                            ccAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCAt                       <  2:127954/149‑1 (MQ=255)
                                                                                                                             cAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATg                      <  3:538404/149‑1 (MQ=255)
                                                                                                                                 aaCAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTTACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCAGCTGGTCAATATGCTCGTCAATCCATGCGAt                  >  2:352108/1‑149 (MQ=255)
                                                                                                                                      gcggcgTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGATAAGAt             <  2:129928/149‑1 (MQ=255)
                                                                                                                                       cggcgTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGATAAGATc            <  3:540012/149‑1 (MQ=255)
                                                                                                                                           gTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGATAAGATCCTGa        >  1:588802/1‑149 (MQ=255)
                                                                                                                                            ttGGCGATTACATTCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCGCTTTGAATAGCCTGATTTTTTTGCGACTACCTCAATGTTAAGCGGCTGGTCAatat                                     <  4:202221/119‑1 (MQ=255)
                                                                                                                                            ttGGCGATTACATTCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAatat                                     >  3:202221/1‑119 (MQ=255)
                                                                                                                                            ttGGCGATTACATTCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGATAAGCTCCTGaa       >  3:555085/1‑149 (MQ=255)
                                                                                                                                              ggCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGATAAGATCCTGaata     >  2:476301/1‑149 (MQ=255)
                                                                                                                                               gCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAAt                                                         <  4:370848/96‑1 (MQ=255)
                                                                                                                                               gCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAAt                                                         >  3:370848/1‑96 (MQ=255)
                                                                                                                                                 gAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGATAAGATCCTGAATAAtt  <  3:345782/149‑1 (MQ=255)
                                                                                                                                                 gAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGATAAGATCCTGAATAAtt  >  2:722709/1‑149 (MQ=255)
                                                                                                                                                 gAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGATAAGATCCTGAATAAtt  >  4:106115/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TAATCGCTGGGAGTGCGATCAAACTGCCGACGGAAAACGCGGGAGAAGGTCTGCTGCGAGACATAACCCAGGTCCATTGCGATATCAAAAATCGGACGCTCGGTGGTGCGCAACTCAACGGCGGCCAGTAACAGGCGGCGTTGGCGAATGTAATCGCCAAGCGTCTGATGCGTCACCGTGCGGAACATTCGTTGCAAGTACCACTTTGAATAGCCTGATTTTTTTGCGACTACATCAATGTTAAGCGGCTGGTCAATATGCTCGTCAATCCATGCGATAAGATCCTGAATAATT  >  NC_000913/4277076‑4277369

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑