Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,317,984 T→A 5.5% intergenic (‑89/+22) phnL ← / ← phnK ribophosphonate triphosphate synthase subunit; putative ABC transporter‑related ATPase/carbon‑phosphorus lyase complex subunit, putative ATP transporter ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,317,9840TA 5.5% 313.8 / 5.7 92intergenic (‑89/+22)phnL/phnKribophosphonate triphosphate synthase subunit; putative ABC transporter‑related ATPase/carbon‑phosphorus lyase complex subunit, putative ATP transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (38/48);  new base A (2/3);  total (40/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.80e-01

CCGTTTTGCTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTTCATCACCAGCAAACGGTCCGCCAG  >  NC_000913/4317843‑4318131
                                                                                                                                             |                                                                                                                                                   
ccGTTTTGCTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTAcgccg                                                                                                                                              <  3:261588/149‑1 (MQ=255)
    tttGCTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATGCGGCGTAAAcgccttatc                                                                                                                                          <  4:9257/149‑6 (MQ=38)
     ttGCTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATcc                                                                                                                                         <  2:499485/149‑1 (MQ=255)
     ttGCTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGATGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATcc                                                                                                                                         <  4:238012/149‑1 (MQ=255)
             gCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCgg                                                                                                                                 >  3:526130/1‑149 (MQ=255)
                 gATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCa                                                                                                                             <  3:349767/149‑1 (MQ=255)
                 gATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCAGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCa                                                                                                                             >  4:667340/1‑149 (MQ=255)
                    gAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAAtt                                                                                                                          <  2:91410/149‑1 (MQ=255)
                    gAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAAtt                                                                                                                          >  4:390093/1‑149 (MQ=255)
                     aaGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTc                                                                                                                         <  3:390093/149‑1 (MQ=255)
                       ggTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTg                                                                                                                       <  1:54915/149‑1 (MQ=255)
                               tGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCg                                                                                                               >  4:119483/1‑149 (MQ=255)
                               tGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCg                                                                                                               >  4:131742/1‑149 (MQ=255)
                                gACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGa                                                                                                              <  1:190991/149‑1 (MQ=255)
                                 aCGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGAt                                                                                                             <  3:587387/149‑1 (MQ=255)
                                 aCGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGAt                                                                                                             <  4:136496/149‑1 (MQ=255)
                                 aCGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGAt                                                                                                             <  1:556975/149‑1 (MQ=255)
                                 aCGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGAt                                                                                                             <  4:700057/149‑1 (MQ=255)
                                         tACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACcag                                                                                                     >  1:272424/1‑149 (MQ=255)
                                         tACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACcag                                                                                                     >  2:624989/1‑149 (MQ=255)
                                            gTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGa                                                                                                              <  1:149886/137‑1 (MQ=255)
                                            gTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGa                                                                                                              >  2:149886/1‑137 (MQ=255)
                                            gTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACcagcag                                                                                                  >  3:728783/1‑149 (MQ=255)
                                            gTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACcagcag                                                                                                  >  2:275945/1‑149 (MQ=255)
                                             ttAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCagcagc                                                                                                 <  3:293161/149‑1 (MQ=255)
                                             ttAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCagcagc                                                                                                 <  4:43670/149‑1 (MQ=255)
                                             ttAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCagcagc                                                                                                 >  2:498871/1‑149 (MQ=255)
                                                aTCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATACGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTGTACGCCGCATCCGGTACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCtgt                                                                                              <  2:208717/149‑1 (MQ=255)
                                                 tCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATGCGGCGTAAACGCCTTATCCGGcct                                                                                                                                    >  4:748090/1‑108 (MQ=21)
                                                 tCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATGCGGCGTAAACGCCTTATCCGGcct                                                                                                                                    <  3:748090/110‑3 (MQ=21)
                                                 tCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACcagca                                                                                                   >  2:643552/1‑143 (MQ=255)
                                                 tCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACcagca                                                                                                   <  1:643552/143‑1 (MQ=255)
                                                 tCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCtgtg                                                                                             >  2:108422/1‑149 (MQ=255)
                                                 tCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCtgtg                                                                                             >  3:561863/1‑149 (MQ=255)
                                                    tttttCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGa                                                                                                           <  2:218598/132‑1 (MQ=255)
                                                    tttttCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGa                                                                                                           >  1:218598/1‑132 (MQ=255)
                                                      tttCGCCTCGTGGTTCGGGAGTCGGATGCGACGCTGACGCGTGTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATAc                                                                                        >  3:445964/1‑149 (MQ=255)
                                                          gCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGgat                                                                                    >  4:178752/1‑149 (MQ=255)
                                                          gCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGgat                                                                                    <  4:647635/149‑1 (MQ=255)
                                                            cTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGgatga                                                                                  >  4:617034/1‑149 (MQ=255)
                                                             tCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGgatgat                                                                                 >  1:567682/1‑149 (MQ=255)
                                                                tGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATGCGGCGTAAACGCCTTATCCggt                                                                                                                                      <  2:657092/93‑2 (MQ=18)
                                                                tGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGAt                                                                                                             <  3:6904/118‑1 (MQ=255)
                                                                tGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGAt                                                                                                             >  4:6904/1‑118 (MQ=255)
                                                                tGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCg                                                                              <  3:618442/149‑1 (MQ=255)
                                                                tGGTTCGGGTGTCGGATGCGACGCGGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                                                                                                      >  1:657092/1‑93 (MQ=18)
                                                                 ggTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCgg                                                                             <  3:76207/149‑1 (MQ=255)
                                                                 ggTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCgg                                                                             <  2:108837/149‑1 (MQ=255)
                                                                 ggTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCgg                                                                             <  2:637630/149‑1 (MQ=255)
                                                                 ggTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCACATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCgg                                                                             <  4:732004/149‑1 (MQ=255)
                                                                  gTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCggg                                                                            >  2:57961/1‑149 (MQ=255)
                                                                         tgtCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGa                                                                     <  2:330551/149‑1 (MQ=255)
                                                                         tgtCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGGCGTCGa                                                                     <  2:443828/149‑1 (MQ=255)
                                                                           tCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGc                                                                   <  4:728783/149‑1 (MQ=255)
                                                                           tCGGAGGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGc                                                                   <  2:563163/149‑1 (MQ=255)
                                                                              gATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCAcg                                                                >  4:744417/1‑149 (MQ=255)
                                                                              gATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCAcg                                                                >  2:513916/1‑149 (MQ=255)
                                                                                   gACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGcggtc                                                           <  2:272424/149‑1 (MQ=255)
                                                                                    aCGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGcggtcg                                                          >  4:746388/1‑149 (MQ=255)
                                                                                    aCGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGcggtcg                                                          >  3:604404/1‑149 (MQ=255)
                                                                                    aCGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGcggtcg                                                          >  4:144263/1‑149 (MQ=255)
                                                                                     cGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGcggtcgg                                                         <  4:526130/149‑1 (MQ=255)
                                                                                        tGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTa                                                      <  2:213808/149‑1 (MQ=255)
                                                                                               tCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCAct                                               >  3:24697/1‑149 (MQ=255)
                                                                                               tCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCAct                                               >  2:753591/1‑149 (MQ=255)
                                                                                                cTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGc                                                                               >  1:172225/1‑116 (MQ=255)
                                                                                                cTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGc                                                                               <  2:172225/116‑1 (MQ=255)
                                                                                                   aTCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACtctcg                                           >  1:684075/1‑148 (MQ=255)
                                                                                                    tCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGcggtcggt                                                        <  3:264577/135‑1 (MQ=255)
                                                                                                    tCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGcggtcggt                                                        >  4:264577/1‑135 (MQ=255)
                                                                                                    tCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGcggtcgg                                                         <  4:708394/134‑1 (MQ=255)
                                                                                                    tCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTcca                                          >  1:697007/1‑149 (MQ=255)
                                                                                                    tCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGAATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGcggtcgg                                                         >  3:708394/1‑134 (MQ=255)
                                                                                                                ggAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCt                              <  1:624989/149‑1 (MQ=255)
                                                                                                                ggAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCt                              <  4:445964/149‑1 (MQ=255)
                                                                                                                  aGCGCATTTGTAGGCCGGATAAGGCGTTTACGTCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTCTACGGATGATGCGGGTCGTCGAGCACTCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGc                            <  3:638811/149‑1 (MQ=255)
                                                                                                                  aGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGCTGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGc                            <  1:498871/149‑1 (MQ=255)
                                                                                                                  aGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGc                            <  4:561863/149‑1 (MQ=255)
                                                                                                                  aGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGc                            <  3:746388/149‑1 (MQ=255)
                                                                                                                     gcATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTtc                         >  4:40124/1‑149 (MQ=255)
                                                                                                                     gcATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTtc                         <  3:144263/149‑1 (MQ=255)
                                                                                                                     gcATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTtc                         <  4:289350/149‑1 (MQ=255)
                                                                                                                       aTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTtcat                       >  3:138007/1‑149 (MQ=255)
                                                                                                                         ttGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTtcatca                     <  1:558449/149‑1 (MQ=255)
                                                                                                                            tAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTTCATCACCa                  <  1:513916/149‑1 (MQ=255)
                                                                                                                            tAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTTCATCACCa                  >  2:127910/1‑149 (MQ=255)
                                                                                                                            tAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTTCATCACCa                  <  3:744417/149‑1 (MQ=255)
                                                                                                                               gCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTTCATCACCAGCa               >  3:31053/1‑149 (MQ=255)
                                                                                                                                       aGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCTGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCGACCACTTGCACCTGCTTTATCACCAGCAAACGGtac       <  4:681583/149‑3 (MQ=255)
                                                                                                                                       aGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTTCATCACCAGCAAACGGTcc       <  4:219545/149‑1 (MQ=255)
                                                                                                                                       aGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTTCATCACCAGCAAACGGTcc       <  4:604404/149‑1 (MQ=255)
                                                                                                                                        ggCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTTCATCACCAGCAAACGGTCCg      >  1:58347/1‑149 (MQ=255)
                                                                                                                                            tttACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTTCATCACCAGCAAACGGTCCGCcag  >  1:416348/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                                   
CCGTTTTGCTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTTCATCACCAGCAAACGGTCCGCCAG  >  NC_000913/4317843‑4318131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: