Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,335,655 T→A 7.8% intergenic (‑65/+39) eptA ← / ← adiC lipid A phosphoethanolamine transferase/arginine:agmatine antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,335,6550TA 7.8% 335.9 / 14.4 101intergenic (‑65/+39)eptA/adiClipid A phosphoethanolamine transferase/arginine:agmatine antiporter
Reads supporting (aligned to +/- strand):  ref base T (51/42);  new base A (6/2);  total (57/44)
Fisher's exact test for biased strand distribution p-value = 4.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.23e-01

Only 100 of 103 total aligned reads displayed.

CAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACAAATGACCACATAA  >  NC_000913/4335507‑4335803
                                                                                                                                                    |                                                                                                                                                    
cAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGtt                                                                                                                                                      <  4:76294/149‑1 (MQ=255)
   gCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCtt                                                                                                                                                   >  2:84170/1‑149 (MQ=255)
    cAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTa                                                                                                                                                  <  2:416978/149‑1 (MQ=255)
     aGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTaa                                                                                                                                                 >  1:682143/1‑149 (MQ=255)
          gagaTATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTg                                                                                                                                            <  2:325273/149‑1 (MQ=255)
            gaTATAAAAAGCGGCCAACAATAGCCAGGCGAGTAATTTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGc                                                                                                                                          >  4:717929/1‑149 (MQ=255)
                 aaaaaGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTa                                                                                                                                     >  3:546176/1‑149 (MQ=255)
                 aaaaaGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTa                                                                                                                                     >  3:440786/1‑149 (MQ=255)
                 aaaaaGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAAAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTa                                                                                                                                     >  4:625477/1‑149 (MQ=255)
                  aaaaGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTAt                                                                                                                                    <  3:327507/149‑1 (MQ=255)
                   aaaGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATg                                                                                                                                   >  4:350847/1‑149 (MQ=255)
                    aaGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGt                                                                                                                                  >  4:561675/1‑149 (MQ=255)
                         gCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCt                                                                                                                             >  3:4099/1‑149 (MQ=255)
                            aacaaTAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGa                                                                                                                          >  1:728316/1‑149 (MQ=255)
                                 tAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAg                                                                                                                     <  1:404363/149‑1 (MQ=255)
                                    ccAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAgggg                                                                                                                  <  3:241826/149‑1 (MQ=255)
                                      aGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGaa                                                                                                                >  4:747363/1‑149 (MQ=255)
                                       ggCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAAt                                                                                                               <  2:388261/149‑1 (MQ=255)
                                          gAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTaa                                                                                                            >  3:75712/1‑149 (MQ=255)
                                           aGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTTCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAAt                                                                                                           <  2:618060/149‑1 (MQ=255)
                                            gTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTaa                                                                                                                      >  1:106148/1‑137 (MQ=255)
                                            gTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTaa                                                                                                                      <  2:106148/137‑1 (MQ=255)
                                            gTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATc                                                                                                          >  4:112293/1‑149 (MQ=255)
                                                aTTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTg                                                                                                      <  3:354779/149‑1 (MQ=255)
                                                aTTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTg                                                                                                      >  4:367391/1‑149 (MQ=255)
                                                 ttCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCGAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGc                                                                                                     <  1:385475/149‑1 (MQ=255)
                                                 ttCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGc                                                                                                     <  2:264666/149‑1 (MQ=255)
                                                     aaGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTAt                                                                                                 >  3:464994/1‑149 (MQ=255)
                                                     aaGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTAt                                                                                                 >  1:407873/1‑149 (MQ=255)
                                                     aaGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTAt                                                                                                 >  4:103727/1‑149 (MQ=255)
                                                      agagGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTAtt                                                                                                <  2:683184/149‑1 (MQ=255)
                                                        agGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTgg                                                                                              <  4:75712/149‑1 (MQ=255)
                                                           gTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGTACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGc                                                                                           >  3:494484/1‑149 (MQ=255)
                                                           gTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTaa                                                                                                                      >  4:136407/1‑122 (MQ=255)
                                                           gTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTaa                                                                                                                      <  3:136407/122‑1 (MQ=255)
                                                              tttttAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATc                                                                                        >  3:696642/1‑149 (MQ=255)
                                                              tttttAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATc                                                                                        >  1:63464/1‑149 (MQ=255)
                                                                 ttAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTaa                                                                                     <  4:183820/149‑1 (MQ=255)
                                                                   agtagGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAgg                                                                                   <  3:126113/149‑1 (MQ=255)
                                                                    gtagGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGATTATTGGTGCATCTAAggg                                                                                  >  4:516884/1‑149 (MQ=255)
                                                                      agGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGAt                                                                                >  1:96229/1‑149 (MQ=255)
                                                                       ggCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATa                                                                               <  1:51266/149‑1 (MQ=255)
                                                                        gcgcTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAGTTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATAc                                                                              <  3:717929/149‑1 (MQ=255)
                                                                          gcTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACgg                                                                            >  3:246098/1‑149 (MQ=255)
                                                                            ttCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGt                                                                          >  2:735248/1‑149 (MQ=255)
                                                                              cAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCGTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGttt                                                                        <  4:696642/149‑1 (MQ=255)
                                                                               aaCATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGtttt                                                                       >  1:535974/1‑149 (MQ=255)
                                                                                  aTCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTAt                                                                    >  2:430856/1‑149 (MQ=255)
                                                                                            ttCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGtt                                                          <  1:433627/149‑1 (MQ=255)
                                                                                            atCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAAGTAATCGTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGAGGGtt                                                          <  2:593539/148‑1 (MQ=255)
                                                                                             tCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTACGTAGCCGGTTg                                                         >  4:402923/1‑149 (MQ=255)
                                                                                               cATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGt                                                                          <  3:49994/130‑1 (MQ=255)
                                                                                               cATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGt                                                                          >  4:49994/1‑130 (MQ=255)
                                                                                                    aaCAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCa                                                  >  4:394476/1‑149 (MQ=255)
                                                                                                    aaCAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCa                                                  <  1:36409/149‑1 (MQ=255)
                                                                                                    aaCAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCa                                                  >  3:577975/1‑149 (MQ=255)
                                                                                                    aaCAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCa                                                  >  1:293913/1‑149 (MQ=255)
                                                                                                      cAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAgc                                                <  3:301074/149‑1 (MQ=255)
                                                                                                      cAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAgc                                                <  1:735248/149‑1 (MQ=255)
                                                                                                          gTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCAt                                            >  1:740119/1‑149 (MQ=255)
                                                                                                               cATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATg                                       >  1:393452/1‑149 (MQ=255)
                                                                                                                atatGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAGGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAAATCAGCGCATACATgg                                      <  2:521865/149‑1 (MQ=255)
                                                                                                                atatGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATgg                                      <  2:227588/149‑1 (MQ=255)
                                                                                                                  atGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTAt                                                                    <  1:235185/117‑1 (MQ=255)
                                                                                                                  atGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTAt                                                                    >  2:235185/1‑117 (MQ=255)
                                                                                                                  atGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCg                                    >  2:645894/1‑149 (MQ=255)
                                                                                                                    gCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGt                                  >  3:384414/1‑149 (MQ=255)
                                                                                                                      tCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGtga                                >  2:577658/1‑149 (MQ=255)
                                                                                                                       cGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGtgat                               <  4:596970/149‑1 (MQ=255)
                                                                                                                         caaagcaaaATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGtgatga                             <  1:430060/149‑1 (MQ=255)
                                                                                                                          aaagcaaaATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGtgat                               >  4:411523/1‑146 (MQ=255)
                                                                                                                          aaagcaaaATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGtgat                               <  3:411523/146‑1 (MQ=255)
                                                                                                                          aaagcaaaATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAACGCATACATGGCGGTGATGAc                            >  3:83728/1‑149 (MQ=255)
                                                                                                                            agcaaaATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCa                          >  3:542776/1‑149 (MQ=255)
                                                                                                                            agcaaaATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCa                          >  3:16164/1‑149 (MQ=255)
                                                                                                                                aaaTTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAg                      >  1:526888/1‑149 (MQ=255)
                                                                                                                                aaaTTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAg                      <  4:577975/149‑1 (MQ=255)
                                                                                                                                 aaTTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAgg                     <  1:695833/149‑1 (MQ=255)
                                                                                                                                 aaTTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAgg                     <  2:527868/149‑1 (MQ=255)
                                                                                                                                  aTTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGTGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGTGGTGATGACCATCAggg                    <  2:682143/149‑1 (MQ=255)
                                                                                                                                  aTTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAggg                    >  3:612090/1‑149 (MQ=255)
                                                                                                                                       gCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACa               >  3:698267/1‑149 (MQ=255)
                                                                                                                                        ccAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACaa              >  4:197630/1‑149 (MQ=255)
                                                                                                                                         cAACCTTAAGAACTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACaaa             >  2:610808/1‑149 (MQ=255)
                                                                                                                                         cAACCTTAAGAACTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACaaa             >  4:293026/1‑149 (MQ=255)
                                                                                                                                          aaCCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACAAAt            <  1:727865/149‑1 (MQ=255)
                                                                                                                                          aaCCTTAAGAACTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCt                                                                                                         <  4:226041/56‑1 (MQ=37)
                                                                                                                                          aaCCTTAAGAACTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCt                                                                                                         >  3:226041/1‑56 (MQ=37)
                                                                                                                                          aaCCTTAAGAACTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCgg                                   >  2:368148/1‑126 (MQ=255)
                                                                                                                                          aaCCTTAAGAACTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCgg                                   <  1:368148/126‑1 (MQ=255)
                                                                                                                                          aaCCTTAAGAACTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACAAAt            >  4:104679/1‑149 (MQ=255)
                                                                                                                                          aaCCTTAAGAACTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACAAAt            >  1:619838/1‑149 (MQ=255)
                                                                                                                                            ccTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACAAATGa          <  3:197630/149‑1 (MQ=255)
                                                                                                                                             cTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACAAATGAc         <  3:561675/149‑1 (MQ=255)
                                                                                                                                             cTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCGAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACAAATGAc         <  4:546176/149‑1 (MQ=255)
                                                                                                                                              ttAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACAAATGAcc        >  1:386861/1‑149 (MQ=255)
                                                                                                                                              ttAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACAAATGAcc        >  1:307597/1‑149 (MQ=255)
                                                                                                                                               tAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACAAATGACca       <  4:246098/149‑1 (MQ=255)
                                                                                                                                                aaGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACAAATGACcac      >  4:738557/1‑149 (MQ=255)
                                                                                                                                                    tCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACAAATGACCACATaa  <  2:535974/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACAAATGACCACATAA  >  NC_000913/4335507‑4335803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: