Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,373,287 G→T 5.3% intergenic (+125/+78) yjeI → / ← yjeJ DUF4156 family lipoprotein/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,373,2870GT 5.3% 332.0 / 7.3 94intergenic (+125/+78)yjeI/yjeJDUF4156 family lipoprotein/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (45/44);  new base T (2/3);  total (47/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.79e-01

Only 100 of 102 total aligned reads displayed.

AGGTTTATAAGTGCCCGAACTGATGTGATCCAGAGGCTGGGTGACAAACGTATAATTGCCTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGCGCGTAAGCATTGA  >  NC_000913/4373140‑4373434
                                                                                                                                                   |                                                                                                                                                   
aGGTTTATAAGTGCCCGAACTGATGTGATCCAGAGGCTGGGTGACAAACGTATAATTGCCTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTAcgccgc                                                                                                                                                    >  3:279213/1‑149 (MQ=255)
   tttATAAGTGCCCGAACTGATGTGATCCAGAGGCTGGGTGACAAACGTATAATTGCCTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATc                                                                                                                                                 >  3:317052/1‑149 (MQ=255)
     tataAGTGACCGAACTGATGTCATCCAGAGGCTGGGTGACAAACGTATAATTGCCTGATGCGGTTCGCTTATCAGGCCTACATAAGGCATAGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCg                                                                                                                                               <  3:223603/149‑1 (MQ=255)
      ataAGTGCCCGAACTGATGTGATCCAGAGGCTGGGTGACAAACGTATAATTGCCTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGGCGCATCCgg                                                                                                                                              >  2:80134/1‑149 (MQ=255)
       taAGTGCCCGAACTGATGTGATCCAGAGGCTGGGTGACAAACGTATAATTGCCTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                                                                                                             <  4:43760/149‑1 (MQ=255)
        aaGTGCCCGAACTGATGTGATCCAGAGGCTGGGTGACAAACGTATAATTGCCTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATGCGGCGTAAACGCCTTATCCGGcc                                                                                                                                            <  2:647433/149‑2 (MQ=38)
         aGTGCCCGAACTGATGTGATCCAGAGGCTGGGTGACAAACGTATAATTGCCTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAt                                                                                                                                           >  3:306559/1‑149 (MQ=255)
          gTGCCCGAACTGATGTGATCCAGAGGCTGGGTGACAAACGTATAATTGCCTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATg                                                                                                                                          >  4:111591/1‑149 (MQ=255)
          gTGCCCGAACTGATGTGATCCAGAGGCTGGGTGACAAACGTATAATTGCCTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATg                                                                                                                                          <  4:415836/149‑1 (MQ=255)
            gCCCGAACTGATGTGATCCAGAGGCTGGGTGACAAACGTATAATTGCCTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAc                                                                                                                                        >  1:764520/1‑149 (MQ=255)
                    tGATGTGATCCAGAGGCTGGGTGACAAACGTATAATTGCCTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATc                                                                                                                                >  2:730493/1‑149 (MQ=255)
                    tGATGTGATCCAGAGGCTGGGTGACAAACGTATAATTGCCTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATc                                                                                                                                >  4:363151/1‑149 (MQ=255)
                      aTGTGATCCAGAGGCTGTGTGACAAACGTATAATTGCCTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATGACCAACGATCAc                                                                                                                              <  4:474645/149‑1 (MQ=255)
                      aTGTGATCCAGAGGCTGGGTGACAAACGTATAATTGCCTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCAc                                                                                                                              <  2:513115/149‑1 (MQ=255)
                          gATCCAGAGGCTGGGTGACAAACGTATAATTGCCTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                                                                                                             <  3:551647/130‑1 (MQ=38)
                          gATCCAGAGGCTGGGTGACAAACGTATAATTGCCTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                                                                                                             >  4:551647/1‑130 (MQ=38)
                           aTCCAGAGGCTGGGTGACAAACGTATAATTGCCTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTAt                                                                                                                         >  2:697424/1‑149 (MQ=255)
                            tCCAGAGGCTGGGTGACAAACGTATAATTGCCTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATc                                                                                                                        >  2:673956/1‑149 (MQ=255)
                            tCCAGAGGCTGGGTGACAAACGTATAATTGCCTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATc                                                                                                                        <  1:80134/149‑1 (MQ=255)
                              cAGAGGCTGGGTGACAAACGTATAATTGCCTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTAt                                                                                                                         >  3:82954/1‑146 (MQ=255)
                                       ggTGACAAACGTATAATTGCCTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAg                                                                                                             <  4:307649/149‑1 (MQ=255)
                                        gTGACAAACGTATAATTGCCTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAgg                                                                                                            >  2:543244/1‑149 (MQ=255)
                                             aaaCGTATAATTGCCTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGa                                                                                                       <  1:299074/149‑1 (MQ=255)
                                              aaCGTATAATTGCCTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGAt                                                                                                      <  1:697424/149‑1 (MQ=255)
                                               aCGTATAATTGCCTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATg                                                                                                     >  1:520069/1‑149 (MQ=255)
                                                 gTATAATTGCCTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGc                                                                                                   >  3:36824/1‑149 (MQ=255)
                                                    taATTGCCTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTcc                                                                                                >  4:247850/1‑149 (MQ=255)
                                                    taATTGCCTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTcc                                                                                                >  2:487324/1‑149 (MQ=255)
                                                      aTTGCCTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAg                                                                                              >  3:698364/1‑149 (MQ=255)
                                                      aTTGCCTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAg                                                                                              <  2:616252/149‑1 (MQ=255)
                                                          ccTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTc                                                                                          <  3:20701/149‑1 (MQ=255)
                                                          ccTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTc                                                                                          <  4:36824/149‑1 (MQ=255)
                                                          ccTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTc                                                                                          <  2:112709/149‑1 (MQ=255)
                                                            tGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGc                                                                                        <  1:601527/149‑1 (MQ=255)
                                                              aTGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCAt                                                                                      >  1:397790/1‑149 (MQ=255)
                                                                gcgcTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTa                                                                                    <  3:545743/149‑1 (MQ=255)
                                                                 cgcttcgcttATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAg                                                                                   <  1:543244/149‑1 (MQ=255)
                                                                  gcttcgcttATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGc                                                                                  >  4:536453/1‑149 (MQ=255)
                                                                  gcttcgcttATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGc                                                                                  >  4:505052/1‑149 (MQ=255)
                                                                        cttATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGAt                                                                            >  4:411573/1‑149 (MQ=255)
                                                                         ttATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATg                                                                           >  3:522259/1‑149 (MQ=255)
                                                                          tATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTCCGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATga                                                                          <  4:448974/149‑1 (MQ=255)
                                                                          tATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATga                                                                          <  4:221064/149‑1 (MQ=255)
                                                                          tATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATga                                                                          <  2:667532/149‑1 (MQ=255)
                                                                            tCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATgaga                                                                        <  4:317052/149‑1 (MQ=255)
                                                                              aGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTGGCATTAGCAAAGATGAGatt                                                                      >  3:622415/1‑149 (MQ=255)
                                                                               ggCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGatta                                                                     <  1:320844/149‑1 (MQ=255)
                                                                                gCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGattat                                                                    >  2:356105/1‑149 (MQ=255)
                                                                                gCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGattat                                                                    >  1:563717/1‑149 (MQ=255)
                                                                                gCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGattat                                                                    >  2:503925/1‑149 (MQ=255)
                                                                                  cTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTa                                                                                    <  1:708355/131‑1 (MQ=255)
                                                                                  cTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTa                                                                                    >  2:708355/1‑131 (MQ=255)
                                                                                  cTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTAttt                                                                  >  2:698774/1‑149 (MQ=255)
                                                                                     cATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGc                                                               <  3:247850/149‑1 (MQ=255)
                                                                                       tAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCt                                                             <  4:698364/149‑1 (MQ=255)
                                                                                       tAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCt                                                             >  3:71085/1‑149 (MQ=255)
                                                                                         aGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTa                                                                                    <  1:292511/124‑1 (MQ=255)
                                                                                         aGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTa                                                                                    >  2:292511/1‑124 (MQ=255)
                                                                                         aGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCtgt                                                           <  4:306559/149‑1 (MQ=255)
                                                                                         aGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCtgt                                                           >  1:343501/1‑149 (MQ=255)
                                                                                          ggCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGc                                                               >  1:705434/1‑144 (MQ=255)
                                                                                          ggCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGc                                                               <  2:705434/144‑1 (MQ=255)
                                                                                          ggCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCtgtg                                                          <  1:743993/149‑1 (MQ=255)
                                                                                            cATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTgtggt                                                        <  2:640730/149‑1 (MQ=255)
                                                                                             aTTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTgtggtg                                                       >  3:217990/1‑149 (MQ=255)
                                                                                               tGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCa                                                     <  3:445977/149‑1 (MQ=255)
                                                                                                    cTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGtttt                                                <  4:267507/149‑1 (MQ=255)
                                                                                                    cTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGtttt                                                <  4:357216/149‑1 (MQ=255)
                                                                                                      gATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGc                                              <  3:536453/149‑1 (MQ=255)
                                                                                                        ttGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCtg                                            >  1:341890/1‑149 (MQ=255)
                                                                                                        ttGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCtg                                            >  2:448242/1‑149 (MQ=255)
                                                                                                           aaTGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCtggtg                                         <  4:50093/149‑1 (MQ=255)
                                                                                                           aaTGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCtggtg                                         >  4:745253/1‑149 (MQ=255)
                                                                                                               cTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGAtt                                     <  4:585446/149‑1 (MQ=255)
                                                                                                               cTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGAtt                                     <  1:254317/149‑1 (MQ=255)
                                                                                                                  gagaATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGaa                                  <  1:503925/149‑1 (MQ=255)
                                                                                                                  gagaATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGaa                                  >  2:765849/1‑149 (MQ=255)
                                                                                                                  gagaATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGaa                                  >  4:719654/1‑149 (MQ=255)
                                                                                                                   agaATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAac                                 >  4:22573/1‑149 (MQ=255)
                                                                                                                     aaTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCtgt                                                           <  4:574307/121‑1 (MQ=255)
                                                                                                                     aaTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCtgt                                                           >  3:574307/1‑121 (MQ=255)
                                                                                                                       tttGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGt                             <  1:487324/149‑1 (MQ=255)
                                                                                                                       tttGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGt                             >  2:662279/1‑149 (MQ=255)
                                                                                                                         tGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTaa                           <  2:397790/149‑1 (MQ=255)
                                                                                                                           tAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGt                         <  2:520069/149‑1 (MQ=255)
                                                                                                                            atgCCGGATGCGGCGTAAACGCCTTATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTg                        >  2:43407/3‑149 (MQ=255)
                                                                                                                              gCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGAt                      <  3:548187/149‑1 (MQ=255)
                                                                                                                              gCCGGATAAGGCGTTTACGCCGCATCCGGCATAACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACGCGTAAGTGAt                      >  4:649431/1‑149 (MQ=255)
                                                                                                                               ccGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATg                     <  4:252136/149‑1 (MQ=255)
                                                                                                                                     aaGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAgcgc               >  3:459476/1‑149 (MQ=255)
                                                                                                                                       ggCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAgcgcgc             <  1:673956/149‑1 (MQ=255)
                                                                                                                                        gCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGcgcgcg            >  1:407550/1‑149 (MQ=255)
                                                                                                                                        gCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGcgcgcg            >  4:147929/1‑149 (MQ=255)
                                                                                                                                               cgccgcATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGCGCGTAAGCAt     <  2:341890/149‑1 (MQ=255)
                                                                                                                                               cgccgcATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGCGCGTAAGCAt     <  4:459476/149‑1 (MQ=255)
                                                                                                                                                gccgcGTCCGGCATGACCAACGATCACGTTATCGGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGCGCGTAAGCAtt    >  1:219493/1‑149 (MQ=255)
                                                                                                                                                gccgcATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGCGCGTAAGCAtt    <  2:526140/149‑1 (MQ=255)
                                                                                                                                                  cgcATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGCGCGTAAGCATTGa  <  1:310179/149‑1 (MQ=255)
                                                                                                                                                  cgcATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGCGCGTAAGCATTGa  <  4:71085/149‑1 (MQ=255)
                                                                                                                                                  cgcATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGCGCGTAAGCATTGa  >  2:58719/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
AGGTTTATAAGTGCCCGAACTGATGTGATCCAGAGGCTGGGTGACAAACGTATAATTGCCTGATGCGCTTCGCTTATCAGGCCTACATAAGGCATTGCAACTGATTGAATGCTTGAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGCGCGTAAGCATTGA  >  NC_000913/4373140‑4373434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: