Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 543,091 G→C 8.7% intergenic (+58/+170) glxK → / ← allE glycerate kinase II/S‑ureidoglycine aminohydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913543,0910GC 8.7% 230.7 / 12.5 69intergenic (+58/+170)glxK/allEglycerate kinase II/S‑ureidoglycine aminohydrolase
Reads supporting (aligned to +/- strand):  ref base G (28/35);  new base C (3/3);  total (31/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.69e-01

TGCTCGCCAGCGGTGAAACCAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAA  >  NC_000913/542948‑543237
                                                                                                                                               |                                                                                                                                                  
tGCTCGCCAGCGGTGAAACCAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCg                                                                                                                                               >  1:628652/1‑149 (MQ=255)
      ccAGCGGTGAAACCAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGCATCCGGCATGa                                                                                                                                         <  4:695946/149‑1 (MQ=255)
      ccAGCGGTGAAACCAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGCATCCGGCATGa                                                                                                                                         <  1:637528/149‑1 (MQ=255)
        aGCGGTGAAACCAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGt                                                                                                                                       <  1:600288/149‑1 (MQ=255)
                 aCCAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATAtcc                                                                                                                              >  2:154774/1‑149 (MQ=255)
                   cAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGCATCCGGCATGAGTa                                                                                                                                      <  4:327594/139‑1 (MQ=255)
                   cAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGCATCCGGCATGAGTa                                                                                                                                      >  3:327594/1‑139 (MQ=255)
                    aaTCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATAtccttc                                                                                                                           >  3:73893/1‑149 (MQ=255)
                    aaTCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATAtccttc                                                                                                                           >  1:737607/1‑149 (MQ=255)
                     aTCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATAtccttcc                                                                                                                          <  2:628652/149‑1 (MQ=255)
                     aTCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATAtccttcc                                                                                                                          <  2:645734/149‑1 (MQ=255)
                      tctcTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATAtccttcct                                                                                                                         <  1:24817/149‑1 (MQ=255)
                        tctTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGa                                                                                                                       >  2:641296/1‑149 (MQ=255)
                           tCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGaaaa                                                                                                                    <  2:380745/149‑1 (MQ=255)
                           tCAACAACGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAATGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGaaaa                                                                                                                    <  1:247910/149‑1 (MQ=255)
                              aCAGCGCGCGAAATATTGGCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGGACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATcg                                                                                                                 <  1:317268/149‑1 (MQ=255)
                                   gcgcgAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCaa                                                                                                            >  4:325193/1‑149 (MQ=255)
                                      cgAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAAtt                                                                                                         <  3:629039/149‑1 (MQ=255)
                                       gAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTc                                                                                                        >  3:83764/1‑149 (MQ=255)
                                        aaaTATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCt                                                                                                       <  2:221406/149‑1 (MQ=255)
                                              tGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTatatat                                                                                                 <  1:84904/149‑1 (MQ=255)
                                                ccTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATAtcc                                                                                                                              >  3:604459/1‑118 (MQ=255)
                                                ccTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATAtcc                                                                                                                              <  4:604459/118‑1 (MQ=255)
                                                 cTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAGGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGc                                                                                              <  2:559759/149‑1 (MQ=255)
                                                       cATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGAt                                                                                        <  4:363760/149‑1 (MQ=255)
                                                        aTTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTAc                                                                                                                                     >  2:468628/1‑103 (MQ=255)
                                                        aTTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTAc                                                                                                                                     <  1:468628/103‑1 (MQ=255)
                                                        aTTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATc                                                                                       <  1:394047/149‑1 (MQ=255)
                                                        aTTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATc                                                                                       >  4:550805/1‑149 (MQ=255)
                                                          tAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCAt                                                                                     <  2:522113/149‑1 (MQ=255)
                                                          tAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCAt                                                                                     <  4:670768/149‑1 (MQ=255)
                                                          tAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCAt                                                                                     <  2:737607/149‑1 (MQ=255)
                                                           aaaaTAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATg                                                                                    <  2:513226/149‑1 (MQ=255)
                                                              aTAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAg                                                                                 >  2:250063/1‑149 (MQ=255)
                                                                  gTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCGTAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCt                                                                             <  3:489051/149‑1 (MQ=255)
                                                                   tCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATAtt                                                                                                <  1:497340/129‑1 (MQ=255)
                                                                   tCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATAtt                                                                                                >  2:497340/1‑129 (MQ=255)
                                                                     aGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGAt                                                                          <  4:6625/149‑1 (MQ=255)
                                                                      gggAATTAAAAACTAACCCTTACCTTTATAGAGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGAACCATATCATTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATa                                                                         >  4:465105/1‑149 (MQ=255)
                                                                         aaTTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCAt                                                                                     <  4:240950/134‑1 (MQ=255)
                                                                         aaTTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCAt                                                                                     >  3:240950/1‑134 (MQ=255)
                                                                              aaaaCTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATaagcgaagc                                                                 >  4:46696/1‑149 (MQ=255)
                                                                                     aCCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAg                                                          <  2:706315/149‑1 (MQ=255)
                                                                                       ccTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGc                                                        >  4:695562/1‑149 (MQ=255)
                                                                                         ttACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTa                                                                                  <  4:388464/121‑1 (MQ=255)
                                                                                         ttACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTa                                                                                  >  3:388464/1‑121 (MQ=255)
                                                                                              tttAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTa                                                 >  3:331524/1‑149 (MQ=255)
                                                                                              tttAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTa                                                 <  1:154774/149‑1 (MQ=255)
                                                                                              tttAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTa                                                 >  3:687050/1‑149 (MQ=255)
                                                                                                    gCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACaaaaa                                           >  1:615562/1‑149 (MQ=255)
                                                                                                      ggATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACaaaaaaa                                         <  1:250063/149‑1 (MQ=255)
                                                                                                            gATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACg                                   >  3:745176/1‑149 (MQ=255)
                                                                                                             aTTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACgg                                  >  3:158403/1‑149 (MQ=255)
                                                                                                             aTTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACgg                                  <  3:695562/149‑1 (MQ=255)
                                                                                                             aTTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACgg                                  >  2:757418/1‑149 (MQ=255)
                                                                                                              tttATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGt                                 >  2:230628/1‑149 (MQ=255)
                                                                                                                    tCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAAc                           <  1:545605/149‑1 (MQ=255)
                                                                                                                       cATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGt                        <  3:325193/149‑1 (MQ=255)
                                                                                                                       cATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGt                        <  2:684036/149‑1 (MQ=255)
                                                                                                                        aTAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGt                        >  3:582282/1‑148 (MQ=255)
                                                                                                                        aTAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGt                        <  4:582282/148‑1 (MQ=255)
                                                                                                                        aTAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTg                       <  3:605260/149‑1 (MQ=255)
                                                                                                                         tAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTgg                      <  4:73893/149‑1 (MQ=255)
                                                                                                                             agTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCaaa                  <  3:46696/149‑1 (MQ=255)
                                                                                                                                       tCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATa        >  4:50277/1‑149 (MQ=255)
                                                                                                                                       tCATGCCGCATCCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATa        >  3:631406/1‑149 (MQ=255)
                                                                                                                                       tCATGCCGCATCCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATa        >  1:65562/1‑149 (MQ=255)
                                                                                                                                           gCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTa    >  1:289372/1‑149 (MQ=255)
                                                                                                                                             cGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTaaa  >  3:413129/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
TGCTCGCCAGCGGTGAAACCAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAA  >  NC_000913/542948‑543237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: