Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 710,170 C→T 8.0% intergenic (+54/+30) chiQ → / ← fur chitosugar‑induced verified lipoprotein/ferric iron uptake regulon transcriptional repressor; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913710,1700CT 8.0% 234.7 / 8.9 75intergenic (+54/+30)chiQ/furchitosugar‑induced verified lipoprotein/ferric iron uptake regulon transcriptional repressor; autorepressor
Reads supporting (aligned to +/- strand):  ref base C (34/35);  new base T (4/2);  total (38/37)
Fisher's exact test for biased strand distribution p-value = 6.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.96e-01

AGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAGTCAGGCGAATGCCATGTTTTGCGGCAATTTCACGCTGA  >  NC_000913/710025‑710317
                                                                                                                                                 |                                                                                                                                                   
agTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCa                                                                                                                                                  >  1:620313/1‑149 (MQ=255)
 gtgtCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAg                                                                                                                                                 <  1:640485/149‑1 (MQ=255)
  tgtCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAgg                                                                                                                                                >  2:127221/1‑149 (MQ=255)
      tGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTgg                                                                                                                                            <  3:50763/149‑1 (MQ=255)
      tGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTgg                                                                                                                                            <  3:729143/149‑1 (MQ=255)
       gCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCTGCGGGTTGGc                                                                                                                                           <  1:265292/149‑1 (MQ=255)
         gTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCtt                                                                                                                                         <  2:311969/149‑1 (MQ=255)
         gTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCtt                                                                                                                                         <  3:217954/149‑1 (MQ=255)
         gTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCtt                                                                                                                                         <  4:489516/149‑1 (MQ=255)
          tGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCttt                                                                                                                                        >  4:71500/1‑149 (MQ=255)
           gCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCtttt                                                                                                                                       <  1:123204/149‑1 (MQ=255)
            cAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTtc                                                                                                                                      >  1:95110/1‑149 (MQ=255)
             aaCGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTtct                                                                                                                                     >  2:769212/1‑149 (MQ=255)
              aCGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTtctc                                                                                                                                    >  1:599765/1‑149 (MQ=255)
              aCGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTtctc                                                                                                                                    <  1:110614/149‑1 (MQ=255)
                  aaaCCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTc                                                                                                                                <  3:86477/149‑1 (MQ=255)
                   aaCCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCa                                                                                                                               <  4:549685/149‑1 (MQ=255)
                             gATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTa                                                                                                                     >  2:393258/1‑149 (MQ=255)
                             gATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTa                                                                                                                     >  3:91604/1‑149 (MQ=255)
                                  ggCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGc                                                                                                                <  4:535486/149‑1 (MQ=255)
                                    cGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCt                                                                                                              <  4:212011/149‑1 (MQ=255)
                                        gAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGt                                                                                                          >  3:41192/1‑149 (MQ=255)
                                        gAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGt                                                                                                          >  1:481884/1‑149 (MQ=255)
                                         aaaGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTg                                                                                                         >  2:349221/1‑149 (MQ=255)
                                          aaGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTgc                                                                                                        <  4:738803/149‑1 (MQ=255)
                                            gCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTgcgc                                                                                                      >  1:697583/1‑149 (MQ=255)
                                             ccGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCa                                                                                                     <  4:41192/149‑1 (MQ=255)
                                                 ttGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGtt                                                                                                 >  4:370967/1‑149 (MQ=255)
                                                     gATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATc                                                                                             <  1:349221/149‑1 (MQ=255)
                                                       tAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCtt                                                                                           >  2:488460/1‑149 (MQ=255)
                                                         aaGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTcg                                                                                         <  4:98699/149‑1 (MQ=255)
                                                         aaGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGCAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTcg                                                                                         <  4:622341/149‑1 (MQ=255)
                                                                  aGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCACGTTTCTTATTTCACTTGCATAAAAAAGCCAACCCGCAGGTTAGCTTTTCTCGATCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCAGCTTCGGGGCAAGCg                                                                                >  4:467036/1‑149 (MQ=255)
                                                                   ggAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGc                                                                               >  4:148439/1‑149 (MQ=255)
                                                                     aaaTACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCt                                                                             <  3:370967/149‑1 (MQ=255)
                                                                         aCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCgg                                                                         <  4:30199/149‑1 (MQ=255)
                                                                           gCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGca                                                                       <  4:693847/149‑1 (MQ=255)
                                                                                 ataaCAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGa                                                                 <  3:549159/149‑1 (MQ=255)
                                                                                 ataaCAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGa                                                                 <  3:594773/149‑1 (MQ=255)
                                                                                     cAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGt                                                             <  1:421551/149‑1 (MQ=255)
                                                                                      aaaTAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTa                                                            >  3:309541/1‑149 (MQ=255)
                                                                                          aaGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGa                                                        <  3:71500/149‑1 (MQ=255)
                                                                                            gTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATa                                                      <  1:754614/149‑1 (MQ=255)
                                                                                                   cTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACtg                                               >  2:213932/1‑149 (MQ=255)
                                                                                                    tGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACtgt                                              >  4:30018/1‑149 (MQ=255)
                                                                                                      tAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCTCAGTGACCGTAAAGATAGAGACTGTgg                                            >  3:91242/1‑149 (MQ=255)
                                                                                                      tAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTgg                                            <  1:74214/149‑1 (MQ=255)
                                                                                                          tctcGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAg                                        <  1:127221/149‑1 (MQ=255)
                                                                                                            tcGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAGTc                                      >  4:132636/1‑149 (MQ=255)
                                                                                                            tcGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAGTc                                      >  3:369997/1‑149 (MQ=255)
                                                                                                             cGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACGGTGACCGTAAAGATAGAGACTGTGGTTAGTCa                                     <  3:208545/149‑1 (MQ=255)
                                                                                                               cTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAGTCAgg                                   <  3:30018/149‑1 (MQ=255)
                                                                                                                ttttCTTATTTCCCTCGCATTAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGTTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTACAGATAGAGACTGTGGTTAGTCAGGc                                  >  2:115029/1‑149 (MQ=255)
                                                                                                                    cTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAGTCAGGCGAAt                              <  1:249385/149‑1 (MQ=255)
                                                                                                                    cTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAGTCAGGCGAAt                              <  2:29351/149‑1 (MQ=255)
                                                                                                                      tATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTACATCATCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAGTCAGGCGAATGc                            >  3:550350/1‑149 (MQ=255)
                                                                                                                            ccTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAGTCAGGCGAATGCCATGtt                      <  1:488460/149‑1 (MQ=255)
                                                                                                                              ttGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAGTCAGGCGAATGCCATGtttt                    <  2:481884/149‑1 (MQ=255)
                                                                                                                                gCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAGTCAGGCGAATGCCATGTTTTGc                  >  1:466542/1‑149 (MQ=255)
                                                                                                                                 cATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCCTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAGTCAGGCGAATGCCATGTTTTGCg                 <  3:551509/149‑1 (MQ=255)
                                                                                                                                  aTAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAGTCAGGCGAATGCCATGTTTTGCgg                >  2:754485/1‑149 (MQ=255)
                                                                                                                                    aaaaaaGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAGTCAGGCGAATGCCATGTTTTGCGGCa              >  3:117625/1‑149 (MQ=255)
                                                                                                                                     aaaaaGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAGTCAGGCGAATGCCATGTTTTGCGGCaa             >  1:367291/1‑149 (MQ=255)
                                                                                                                                     aaaaaGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAGTCAGGCGAATGCCATGTTTTGCGGCaa             >  1:462082/1‑149 (MQ=255)
                                                                                                                                      aaaaGCCAACCTGCGGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAGTCAGGCGAATGCCATGTTTTGCGGCAAt            >  2:163720/1‑149 (MQ=255)
                                                                                                                                        aaGCCAACCTGCGGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGcac                                                                      <  4:162245/89‑1 (MQ=255)
                                                                                                                                        aaGCCAACCTGCGGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGcac                                                                      >  3:162245/1‑89 (MQ=255)
                                                                                                                                         aGCCAACCTGCGGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAGTCAGGCGAATGCCATGTTTTGCGGCAATTTc         >  2:773952/1‑149 (MQ=255)
                                                                                                                                          gCCAACCTGCGGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAGTCAGGCGAATGCCATGTTTTGCGGCAATTTCa        >  1:645851/1‑149 (MQ=255)
                                                                                                                                          gCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTaaa                                                          >  2:297589/1‑99 (MQ=255)
                                                                                                                                          gCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTaaa                                                          <  1:297589/99‑1 (MQ=255)
                                                                                                                                          gCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAGTCAGGCGAATGCCATGTTTTGCGGCAATTTCa        >  3:135772/1‑149 (MQ=255)
                                                                                                                                          gCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAGTCAGGCGAATGCCATGTTTTGCGGCAATTTCa        >  2:580086/1‑149 (MQ=255)
                                                                                                                                           ccAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAGTCAGGCGAATGCCATGTTTTGCGGCAATTTCAc       >  4:118761/1‑149 (MQ=255)
                                                                                                                                             aaCCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAGTCAGGCGAATGCCATGTTTTGCGGCAATTTCACGc     >  3:410845/1‑149 (MQ=255)
                                                                                                                                                ccGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAGTCAGGCGAATGCCATGTTTTGCGGCAATTTCACGCTGa  <  1:769212/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
AGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAGTCAGGCGAATGCCATGTTTTGCGGCAATTTCACGCTGA  >  NC_000913/710025‑710317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: