Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 832,379 C→T 6.5% intergenic (+143/+89) rhlE → / ← ybiA ATP‑dependent RNA helicase/DUF1768 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913832,3790CT 6.5% 194.5 / 3.0 61intergenic (+143/+89)rhlE/ybiAATP‑dependent RNA helicase/DUF1768 family protein
Reads supporting (aligned to +/- strand):  ref base C (32/25);  new base T (2/2);  total (34/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01

CAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCTT  >  NC_000913/832231‑832527
                                                                                                                                                    |                                                                                                                                                    
cAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTAcgccgc                                                                                                                                                      <  3:520151/149‑1 (MQ=255)
     aTCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCg                                                                                                                                                 >  4:141976/1‑149 (MQ=255)
      tCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATTTATTGAATTTGCATGCATTTGTAGGCCGGATACGGCGTTTACGCCGCATCCgg                                                                                                                                                >  3:615427/1‑149 (MQ=255)
      tCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCgg                                                                                                                                                >  1:240447/1‑149 (MQ=255)
       cTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                                                                                                               <  1:55402/149‑1 (MQ=25)
        ttttATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCa                                                                                                                                              >  1:518538/1‑149 (MQ=255)
         tttATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAt                                                                                                                                             <  2:153184/149‑1 (MQ=255)
            aTGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTc                                                                                                                                          <  2:56479/149‑1 (MQ=255)
                cGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCacaa                                                                                                                                      <  4:246379/149‑1 (MQ=255)
                           cGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGt                                                                                                                           <  2:27964/149‑1 (MQ=255)
                             gCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCa                                                                                                                         <  2:289503/149‑1 (MQ=255)
                             gCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCa                                                                                                                         <  2:180331/149‑1 (MQ=255)
                                     cTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTg                                                                                                                 <  2:167593/149‑1 (MQ=255)
                                           aaCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAaca                                                                                                           >  1:104515/1‑149 (MQ=255)
                                            aCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAacac                                                                                                          <  2:605300/149‑1 (MQ=255)
                                              aaaCGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTga                                                                                                                <  3:330340/141‑1 (MQ=255)
                                              aaaCGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTga                                                                                                                >  4:330340/1‑141 (MQ=255)
                                                    aaaaCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGaaaaa                                                                                                  >  3:404800/1‑149 (MQ=255)
                                                     aaaCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGaaaaaa                                                                                                 <  3:50632/149‑1 (MQ=255)
                                                     aaaCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGaaaaaa                                                                                                 >  1:650316/1‑149 (MQ=255)
                                                     aaaCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGaaaaaa                                                                                                 <  3:141976/149‑1 (MQ=255)
                                                       aCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAAtt                                                                                               <  1:539436/149‑1 (MQ=255)
                                                         tGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTa                                                                                             <  4:615427/149‑1 (MQ=255)
                                                           ccTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATc                                                                                           >  3:103968/1‑149 (MQ=255)
                                                                 gcgcTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGtttt                                                                                     <  3:574839/149‑1 (MQ=255)
                                                                   gcTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTAc                                                                                   >  2:470994/1‑149 (MQ=255)
                                                                        gCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGccc                                                                              >  1:10948/1‑149 (MQ=255)
                                                                            aTCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATaa                                                                          <  2:650316/149‑1 (MQ=255)
                                                                             tCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACtt                                                                                                                             >  2:775271/1‑97 (MQ=255)
                                                                             tCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACtt                                                                                                                             <  1:775271/97‑1 (MQ=255)
                                                                               aGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGcc                                                                       >  1:505047/1‑149 (MQ=255)
                                                                               aGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGcc                                                                       >  2:629660/1‑149 (MQ=255)
                                                                                   cTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCg                                                                   >  4:669354/1‑149 (MQ=255)
                                                                                      cGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCgaga                                                                <  2:107055/149‑1 (MQ=255)
                                                                                             tctGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACt                                                         >  4:180887/1‑149 (MQ=255)
                                                                                             tctGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACt                                                         >  4:575867/1‑149 (MQ=255)
                                                                                                gCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTtct                                                      <  3:575867/149‑1 (MQ=255)
                                                                                                  aaTATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTctct                                                    <  3:271686/149‑1 (MQ=255)
                                                                                                     atatTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCtata                                                 >  2:640472/1‑149 (MQ=255)
                                                                                                     atatTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCtata                                                 <  4:668281/149‑1 (MQ=255)
                                                                                                        ttGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGcc                                              >  4:527619/1‑149 (MQ=255)
                                                                                                        ttGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGcc                                              >  4:100056/1‑149 (MQ=255)
                                                                                                          gAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCaa                                            >  4:682533/1‑149 (MQ=255)
                                                                                                            aTTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAAtt                                          <  2:10948/149‑1 (MQ=255)
                                                                                                               tGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGtt                                       <  3:142798/149‑1 (MQ=255)
                                                                                                                 cATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTcg                                     >  1:192188/1‑149 (MQ=255)
                                                                                                                    gCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCgcgc                                  >  4:531539/1‑149 (MQ=255)
                                                                                                                      ttttGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCaa                                >  3:494231/1‑149 (MQ=255)
                                                                                                                      ttttGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCaa                                >  4:307271/1‑149 (MQ=255)
                                                                                                                      ttttGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCaa                                <  4:505196/149‑1 (MQ=255)
                                                                                                                           aatgCCGGATGCGGCGTAAACGCCTTATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCAACTCCa                           >  1:313178/4‑149 (MQ=255)
                                                                                                                              gCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCAACTCCATTa                        >  2:104415/1‑149 (MQ=255)
                                                                                                                                  gatgcggCGTAAACGCCTTATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTtct                                                      <  4:80503/110‑1 (MQ=255)
                                                                                                                                  gatgcggCGTAAACGCCTTATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTtct                                                      >  3:80503/6‑115 (MQ=255)
                                                                                                                                  gATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGtt                                       >  3:629259/1‑130 (MQ=255)
                                                                                                                                  gATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGtt                                       <  4:629259/130‑1 (MQ=255)
                                                                                                                                      aGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCAACTCCATTaaaa                     >  1:55916/1‑144 (MQ=255)
                                                                                                                                      aGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCAACTCCATTaaaa                     <  2:55916/144‑1 (MQ=255)
                                                                                                                                      aGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCAACTCCATTAAAAGGTAg                >  4:451780/1‑149 (MQ=255)
                                                                                                                                      aGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCAACTCCATTAAAAGGTAg                >  3:336525/1‑149 (MQ=255)
                                                                                                                                        gCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCAACTCCATTAAAAGGTAGcc              >  3:461580/1‑149 (MQ=255)
                                                                                                                                          gTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCa            >  1:647001/1‑149 (MQ=255)
                                                                                                                                                gccgcATCCGGCATTTCACAACAAGCACTTGTCAGCCATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGAt      >  1:772285/1‑149 (MQ=255)
                                                                                                                                                    cATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCtt  <  2:505047/149‑1 (MQ=255)
                                                                                                                                                    cATCCGGCAGTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAGATTTATCTGTTTTACCACCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCtt  <  4:748141/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCAACTCCATTAAAAGGTAGCCCAGTCGATTCTT  >  NC_000913/832231‑832527

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: