Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,229,438 Δ1 bp 6.2% intergenic (+76/+15) yjbB → / ← pepE putative Na+/Pi‑cotransporter/peptidase E, alpha‑aspartyl dipeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,229,4380C. 6.2% 223.5 / 8.5 65intergenic (+76/+15)yjbB/pepEputative Na+/Pi‑cotransporter/peptidase E, alpha‑aspartyl dipeptidase
Reads supporting (aligned to +/- strand):  ref base C (32/29);  new base . (2/2);  total (34/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.83e-01

GCTGTTTTGTTCGGTGGCTTACAGTGTGCTGGAACAGCCGGATGAAGATGAAGGACGGGACGAGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAACACATAAGTGGTGTTCGGGCCACCCAGCGTAGCGTGACCTTTACTCACTGTGATCCAGTTACCTTCCGGTAGACCAATAATCG  >  NC_000913/4229293‑4229586
                                                                                                                                                  |                                                                                                                                                    
gCTGTTTTGTTCGGTGGCTTACAGTGTGCTGGAACAGCCGGATGAAGATGAAGGACGGGACGAGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATc                                                                                                                                                   <  2:433618/149‑1 (MQ=255)
 cTGTTTTGTTCGGTGGCTTACAGTGTGCTGGAACAGCCGGATGAAGATGAAGGACGGGACGAGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATcc                                                                                                                                                  >  2:21693/1‑149 (MQ=255)
    ttttGTTCGGTGGCTTACAGTGTGCTGGAACAGCCGGATGAAGATGAAGGACGGGACGAGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATGCATGATCCGGc                                                                                                                                               <  2:367241/149‑1 (MQ=255)
    ttttGTTCGGTGGCTTACAGTGTGCTGGAACAGCCGGATGAAGATGAAGGACGGGACGAGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGc                                                                                                                                               >  4:369975/1‑149 (MQ=255)
    ttttGTGCGGTGGCTTACAGCGTGCTCGAACAGCCTGATGAAGATGAAGGACGGGACGAGGATTAATATTGTGAAACCTATAGGCCTGATAAGACGCGTCAAGTGTCGCATCAGGCACTATAAACAAGATTCCGGATCATTCATCCGGc                                                                                                                                               >  4:34207/1‑149 (MQ=38)
           cGGTGGCTTACAGTGTGCTGGAACAGCCGGATGAAGATGAAGGACGGGACGAGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCAc                                                                                                                                        <  3:182017/149‑1 (MQ=255)
           cGGTGGCTTACAGTGTGCTGGAACAGCCGGATGAAGATGAAGGACGGGACGAGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCAc                                                                                                                                        <  2:343209/149‑1 (MQ=255)
           cGGTGGCTTACAGTGTGCTGGAACAGCCGGATGAAGATGAAGGACGGGACGAGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGGCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCGGGCAATTCAc                                                                                                                                        <  4:2779/149‑1 (MQ=255)
            ggTGGCTTACAGTGTGCTGGAACAGCCGGATGAAGATGAAGGACGGGACGAGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATGCATGATCCGGCatcttgtt                                                                                                                                       <  2:135486/149‑8 (MQ=38)
              tGGCTTACAGTGTGCTGGAACAGCCGGATGAAGATGAAGGACGGGACGAGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAAtt                                                                                                                                           <  1:359289/143‑1 (MQ=255)
              tGGCTTACAGTGTGCTGGAACAGCCGGATGAAGATGAAGGACGGGACGAGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAAtt                                                                                                                                           >  2:359289/1‑143 (MQ=255)
                gCTTACAGTGTGCTGGAACAGCCGGATGAAGATGAAGGACGGGACGAGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTaaa                                                                                                                                   >  3:263451/1‑149 (MQ=255)
                  ttACAGTGTGCTGGAACAGCCGGATGAAGATGAAGGACGGGACGAGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTaaaaa                                                                                                                                 >  4:119331/1‑149 (MQ=255)
                     cAGTGTGCTGGAACAGCCGGATGAAGATGAAGGACGGGACGAGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACgg                                                                                                                              <  3:124291/149‑1 (MQ=255)
                      aGTGTGCTGGAACAGCCGGATGAAGATGAAGGACGGGACGAGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGt                                                                                                                             <  1:436416/149‑1 (MQ=255)
                         gtgCTGGAACAGCCGGATGAAGATGAAGGACGGGACGAGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGAc                                                                                                                          <  1:301599/149‑1 (MQ=255)
                            cTGGAACAGCCGGATGAAGATGAAGGACGGGACGAGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAg                                                                                                                       >  3:557444/1‑149 (MQ=255)
                              ggAACAGCCGGATGAAGATGAAGGACGGGACGAGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCt                                                                                                                     <  4:263451/149‑1 (MQ=255)
                              ggAACAGCCGGATGAAGATGAAGGACGGGACGAGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCt                                                                                                                     >  2:38213/1‑149 (MQ=255)
                               gAACAGCCGGATGAAGATGAAGGACGGGACGAGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCtt                                                                                                                    >  4:515401/1‑149 (MQ=255)
                               gAACAGCCGGATGAAGATGAAGGACGGGACGAGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCtt                                                                                                                    >  2:546672/1‑149 (MQ=255)
                                        gatgaagatgaaGGACGGGACGAGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCAc                                                                                                                                        >  1:430278/1‑120 (MQ=255)
                                        gatgaagatgaaGGACGGGACGAGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGAATCATGCATCCGGCAATTCAc                                                                                                                                        <  2:430278/120‑1 (MQ=255)
                                          tgaagatgaagGACGGGACGAGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAAcc                                                                                                         >  3:194130/1‑149 (MQ=255)
                                              gatgaagGACGGGACGAGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGctt                                                                                                     >  3:213494/1‑149 (MQ=255)
                                                 gaagGACGGGACGAGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCTGGATCATGCATCAGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGcttctt                                                                                                  <  4:557444/149‑1 (MQ=255)
                                                    ggacgggacgAGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCAc                                                                                               <  1:321901/149‑1 (MQ=255)
                                                     gacgggacgAGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCAcc                                                                                              >  2:260930/1‑149 (MQ=255)
                                                      acgggacgAGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCa                                                                                             <  1:202348/149‑1 (MQ=255)
                                                          gacgAGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCt                                                                                         >  3:510969/1‑149 (MQ=255)
                                                              aGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTaaa                                                                                     <  3:384768/149‑1 (MQ=255)
                                                              aGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTaaa                                                                                     <  1:21693/149‑1 (MQ=255)
                                                                 aTTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAacac                                                                                  >  1:260025/1‑149 (MQ=255)
                                                                  ttAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAAcaca                                                                                 >  2:554515/1‑149 (MQ=255)
                                                                     atatTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAACACATaa                                                                              <  3:212013/149‑1 (MQ=255)
                                                                     atatTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAACACATaa                                                                              <  4:550224/149‑1 (MQ=255)
                                                                      tatTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAACACATAAg                                                                             >  4:249120/1‑149 (MQ=255)
                                                                          gtgAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAACACATAAgtggt                                                                         <  4:459873/149‑1 (MQ=255)
                                                                           tgAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAACACATAAgtggtg                                                                        <  1:74723/149‑1 (MQ=255)
                                                                            gAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAACACATAAGTGgtgt                                                                       >  1:303415/1‑149 (MQ=255)
                                                                            gAATACTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCCGCTTCCAGCGGAACCGCTTCTTCACAAGCCTTAAACACATAAGTGgtgt                                                                       >  4:205211/1‑149 (MQ=255)
                                                                             aaTCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAACACATAAGTGGTGtt                                                                      >  1:451029/1‑149 (MQ=255)
                                                                                ccTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAACACATAAGTGGTGTTCgg                                                                   <  4:369696/149‑1 (MQ=255)
                                                                                     aGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTTACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAACACATAAGTGGTGTTCGGGCCAc                                                              <  3:66148/149‑1 (MQ=255)
                                                                                         cTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAACACATAAgtg                                                                           <  3:144585/132‑1 (MQ=255)
                                                                                         cTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAACACATAAgtg                                                                           >  4:144585/1‑132 (MQ=255)
                                                                                         cTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAACACATAAGTGGTGTTCGGGCCACCCag                                                          >  1:200726/1‑149 (MQ=255)
                                                                                                   gcgTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAACACATAAGTGGTGTTCGGGCCACCCAGCGTAGCGTGa                                                >  4:264616/1‑149 (MQ=255)
                                                                                                     gTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAACACATAAGTGGTGTTCGGGCCACCCAGCGTAGCGTGAcc                                              >  2:1767/1‑149 (MQ=255)
                                                                                                                cATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAACACATAAGTGGTGTTCGGGCCACCCAGCGTAGCGTGACCTTTACTCACtg                                   >  3:211221/1‑149 (MQ=255)
                                                                                                                   cAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAACACATAAGTGGTGTTCGGGCCACCCAGCGTAGCGTGACCTTTACTCACTGTGa                                <  1:260930/149‑1 (MQ=255)
                                                                                                                   cAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAACACATAAGTGGTGTTCGGGCCACCCAGCGTAGCGTGACCTTTACTCACTGTGa                                <  1:47989/149‑1 (MQ=255)
                                                                                                                     ggCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAACACATAAGTGGTGTTCGGGCCACCCAGCGTAGCGTGACCTTTACTCACTGTGATc                              <  2:444184/149‑1 (MQ=255)
                                                                                                                      gCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAACACATAAGTGGTGTTCGGGCCACCCAGCGTAGCGTGACCTTTACTCACTGTGATcc                             >  1:293974/1‑149 (MQ=255)
                                                                                                                       cACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAACACATAAGTGGTGTTCGGGCCACCCAGCGTAGCGTGACCTTTACTCACTGTGATCCa                            <  3:264616/149‑1 (MQ=255)
                                                                                                                          tATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAACACATAAgtgg                                                                          >  4:105780/1‑100 (MQ=255)
                                                                                                                          tATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAACACATAAgtgg                                                                          <  3:105780/100‑1 (MQ=255)
                                                                                                                          tATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAACACATAAGTGGTGTTCGGGCCACCCAGCGTAGCGTGACCTTTACTCACTGTGATCCAGtt                         >  1:452196/1‑149 (MQ=255)
                                                                                                                          tATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAACACATAAGTGGTGTTCGGGCCACCCAGCGTAGCGTGACCTTTACTCACTGTGATCCAGtt                         >  2:47994/1‑149 (MQ=255)
                                                                                                                          tATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAACACATAAGTGGTGTTCGGGCCACCCAGCGTAGCGTGACCTTTACTCACTGTGATCCAGtt                         <  1:546672/149‑1 (MQ=255)
                                                                                                                               gtgaattGCCGGATGCATGATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAACACATAAGTGGTGTTCGGGCCACCCAGCGTAGCGTGACCTTTACTCACTGTGATCCAGTTACCtt                    >  4:328451/7‑149 (MQ=255)
                                                                                                                               aCAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAACACATAAGTGGTGTTCGGGCCACCCAGCGTAGCGTGACCTTTACTCACTGTGATCCAGTTACCtt                    >  4:465681/1‑149 (MQ=255)
                                                                                                                                    ttGCCGGATGCATGATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAACACATAAGTGGTGTTCGGGCCACCCAGCGTAGCGTGACCTTTACTCACTGTGATCCAGTTACCTTCCGGt               >  4:405025/2‑149 (MQ=255)
                                                                                                                                     tGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAACACATAAGTGGTGTTCGGGCCACCCAGCGTAGCGTGACCTTTACTCACTGTGATCCAGTTACCTTCCGGTa              <  4:198934/149‑1 (MQ=255)
                                                                                                                                                tGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAACACATAAGTGGTGTTCGGGCCACCCAGCGTAGCGTGACCTTTACTCACTGTGATCCAGTTACCTTCCGGTAGACCaataat    >  3:211038/1‑149 (MQ=255)
                                                                                                                                                  cATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAACACATAAGTGGTGTTCGGGCCACCCAGCGTAGCGTGACCTTTACTCACTGTGATCCAGTTACCTTCCGGTAGACCAATAATCg  <  2:200726/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GCTGTTTTGTTCGGTGGCTTACAGTGTGCTGGAACAGCCGGATGAAGATGAAGGACGGGACGAGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAACACATAAGTGGTGTTCGGGCCACCCAGCGTAGCGTGACCTTTACTCACTGTGATCCAGTTACCTTCCGGTAGACCAATAATCG  >  NC_000913/4229293‑4229586

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: