Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,229,433:1 +G 6.0% intergenic (+71/+20) yjbB → / ← pepE putative Na+/Pi‑cotransporter/peptidase E, alpha‑aspartyl dipeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,229,4331.G 6.0% 290.3 / 12.9 67intergenic (+71/+20)yjbB/pepEputative Na+/Pi‑cotransporter/peptidase E, alpha‑aspartyl dipeptidase
Reads supporting (aligned to +/- strand):  ref base . (32/31);  new base G (2/2);  total (34/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.32e-01

CTGAACTCGCTGTTTTGTTCGGTGGCTTACAGTGTGCTGGAACAGCCGGATGAAGATGAAGGACGGGACGAGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAACACATAAGTGGTGTTCGGGCCACCCAGCGTAGCGTGACCTTTACTCACTGTGATCCAGTTACCTTCCGGTA  >  NC_000913/4229285‑4229574
                                                                                                                                                     |                                                                                                                                             
cTGAACTCGCTGTTTTGTTCGGTGGCTTACAGTGTGCTGGAACAGCCGGATGAAGATGAAGGACGGGACGAGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGAt                                                                                                                                                <  3:580634/149‑1 (MQ=255)
   aaCTCGCTGTTTTGTTCGGTGGCTTACAGTGTGCTGGAACAGCCGGATGAAGATGAAGGACGGGACGAGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCAt                                                                                                                                            <  1:593937/149‑1 (MQ=255)
   aaCTCGCTGTTTTGTTCGGTGGCTTACAGTGTGCTGGAACAGCCGGATGAAGATGAAGGACGGGACGAGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCAt                                                                                                                                            <  2:415921/149‑1 (MQ=255)
   aaCTCGCTGTTTTGTTCGGTGGCTTACAGTGTGCTGGAACAGCCGGATGAAGATGAAGGACGGGACGAGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCAt                                                                                                                                            >  3:550224/1‑149 (MQ=255)
        gCTGTTTTGTTCGGTGGCTTACAGTGTGCTGGAACAGCCGGATGAAGATGAAGGACGGGACGAGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATc                                                                                                                                       <  2:433618/149‑1 (MQ=255)
         cTGTTTTGTTCGGTGGCTTACAGTGTGCTGGAACAGCCGGATGAAGATGAAGGACGGGACGAGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATcc                                                                                                                                      >  2:21693/1‑149 (MQ=255)
            ttttGTTCGGTGGCTTACAGTGTGCTGGAACAGCCGGATGAAGATGAAGGACGGGACGAGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATGCATGATCCGGc                                                                                                                                   <  2:367241/149‑1 (MQ=255)
            ttttGTTCGGTGGCTTACAGTGTGCTGGAACAGCCGGATGAAGATGAAGGACGGGACGAGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGc                                                                                                                                   >  4:369975/1‑149 (MQ=255)
            ttttGTGCGGTGGCTTACAGCGTGCTCGAACAGCCTGATGAAGATGAAGGACGGGACGAGGATTAATATTGTGAAACCTATAGGCCTGATAAGACGCGTCAAGTGTCGCATCAGGCACTATAAACAAGATTCCGGATCATTCATCCGGc                                                                                                                                   >  4:34207/1‑149 (MQ=38)
                   cGGTGGCTTACAGTGTGCTGGAACAGCCGGATGAAGATGAAGGACGGGACGAGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCAc                                                                                                                            <  2:343209/149‑1 (MQ=255)
                   cGGTGGCTTACAGTGTGCTGGAACAGCCGGATGAAGATGAAGGACGGGACGAGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCAc                                                                                                                            <  3:182017/149‑1 (MQ=255)
                   cGGTGGCTTACAGTGTGCTGGAACAGCCGGATGAAGATGAAGGACGGGACGAGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGGCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCGGGCAATTCAc                                                                                                                            <  4:2779/149‑1 (MQ=255)
                    ggTGGCTTACAGTGTGCTGGAACAGCCGGATGAAGATGAAGGACGGGACGAGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATGCATGATCCGGCatcttgtt                                                                                                                           <  2:135486/149‑8 (MQ=38)
                      tGGCTTACAGTGTGCTGGAACAGCCGGATGAAGATGAAGGACGGGACGAGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAAtt                                                                                                                               >  2:359289/1‑143 (MQ=255)
                      tGGCTTACAGTGTGCTGGAACAGCCGGATGAAGATGAAGGACGGGACGAGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAAtt                                                                                                                               <  1:359289/143‑1 (MQ=255)
                        gCTTACAGTGTGCTGGAACAGCCGGATGAAGATGAAGGACGGGACGAGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTaaa                                                                                                                       >  3:263451/1‑149 (MQ=255)
                          ttACAGTGTGCTGGAACAGCCGGATGAAGATGAAGGACGGGACGAGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTaaaaa                                                                                                                     >  4:119331/1‑149 (MQ=255)
                             cAGTGTGCTGGAACAGCCGGATGAAGATGAAGGACGGGACGAGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACgg                                                                                                                  <  3:124291/149‑1 (MQ=255)
                              aGTGTGCTGGAACAGCCGGATGAAGATGAAGGACGGGACGAGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGt                                                                                                                 <  1:436416/149‑1 (MQ=255)
                                 gtgCTGGAACAGCCGGATGAAGATGAAGGACGGGACGAGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGAc                                                                                                              <  1:301599/149‑1 (MQ=255)
                                    cTGGAACAGCCGGATGAAGATGAAGGACGGGACGAGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAg                                                                                                           >  3:557444/1‑149 (MQ=255)
                                      ggAACAGCCGGATGAAGATGAAGGACGGGACGAGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCt                                                                                                         >  2:38213/1‑149 (MQ=255)
                                      ggAACAGCCGGATGAAGATGAAGGACGGGACGAGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCt                                                                                                         <  4:263451/149‑1 (MQ=255)
                                       gAACAGCCGGATGAAGATGAAGGACGGGACGAGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCtt                                                                                                        >  4:515401/1‑149 (MQ=255)
                                       gAACAGCCGGATGAAGATGAAGGACGGGACGAGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCtt                                                                                                        >  2:546672/1‑149 (MQ=255)
                                                gatgaagatgaaGGACGGGACGAGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCAc                                                                                                                            >  1:430278/1‑120 (MQ=255)
                                                gatgaagatgaaGGACGGGACGAGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGAATCATGCATCCGGCAATTCAc                                                                                                                            <  2:430278/120‑1 (MQ=255)
                                                  tgaagatgaagGACGGGACGAGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAAcc                                                                                             >  3:194130/1‑149 (MQ=255)
                                                      gatgaagGACGGGACGAGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGctt                                                                                         >  3:213494/1‑149 (MQ=255)
                                                         gaagGACGGGACGAGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCTGGATCATGCATCAGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGcttctt                                                                                      <  4:557444/149‑1 (MQ=255)
                                                            ggacgggacgAGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCAc                                                                                   <  1:321901/149‑1 (MQ=255)
                                                             gacgggacgAGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCAcc                                                                                  >  2:260930/1‑149 (MQ=255)
                                                              acgggacgAGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCa                                                                                 <  1:202348/149‑1 (MQ=255)
                                                                  gacgAGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCt                                                                             >  3:510969/1‑149 (MQ=255)
                                                                      aGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTaaa                                                                         <  1:21693/149‑1 (MQ=255)
                                                                      aGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTaaa                                                                         <  3:384768/149‑1 (MQ=255)
                                                                         aTTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAacac                                                                      >  1:260025/1‑149 (MQ=255)
                                                                          ttAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAAcaca                                                                     >  2:554515/1‑149 (MQ=255)
                                                                             atatTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAACACATaa                                                                  <  4:550224/149‑1 (MQ=255)
                                                                             atatTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAACACATaa                                                                  <  3:212013/149‑1 (MQ=255)
                                                                              tatTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAACACATAAg                                                                 >  4:249120/1‑149 (MQ=255)
                                                                                  gtgAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAACACATAAgtggt                                                             <  4:459873/149‑1 (MQ=255)
                                                                                   tgAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAACACATAAgtggtg                                                            <  1:74723/149‑1 (MQ=255)
                                                                                    gAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAACACATAAGTGgtgt                                                           >  1:303415/1‑149 (MQ=255)
                                                                                    gAATACTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCCGCTTCCAGCGGAACCGCTTCTTCACAAGCCTTAAACACATAAGTGgtgt                                                           >  4:205211/1‑149 (MQ=255)
                                                                                     aaTCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAACACATAAGTGGTGtt                                                          >  1:451029/1‑149 (MQ=255)
                                                                                        ccTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAACACATAAGTGGTGTTCgg                                                       <  4:369696/149‑1 (MQ=255)
                                                                                             aGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTTACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAACACATAAGTGGTGTTCGGGCCAc                                                  <  3:66148/149‑1 (MQ=255)
                                                                                                 cTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAACACATAAgtg                                                               >  4:144585/1‑132 (MQ=255)
                                                                                                 cTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAACACATAAgtg                                                               <  3:144585/132‑1 (MQ=255)
                                                                                                 cTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAACACATAAGTGGTGTTCGGGCCACCCag                                              >  1:200726/1‑149 (MQ=255)
                                                                                                           gcgTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAACACATAAGTGGTGTTCGGGCCACCCAGCGTAGCGTGa                                    >  4:264616/1‑149 (MQ=255)
                                                                                                             gTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAACACATAAGTGGTGTTCGGGCCACCCAGCGTAGCGTGAcc                                  >  2:1767/1‑149 (MQ=255)
                                                                                                                        cATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAACACATAAGTGGTGTTCGGGCCACCCAGCGTAGCGTGACCTTTACTCACtg                       >  3:211221/1‑149 (MQ=255)
                                                                                                                           cAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAACACATAAGTGGTGTTCGGGCCACCCAGCGTAGCGTGACCTTTACTCACTGTGa                    <  1:47989/149‑1 (MQ=255)
                                                                                                                           cAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAACACATAAGTGGTGTTCGGGCCACCCAGCGTAGCGTGACCTTTACTCACTGTGa                    <  1:260930/149‑1 (MQ=255)
                                                                                                                             ggCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAACACATAAGTGGTGTTCGGGCCACCCAGCGTAGCGTGACCTTTACTCACTGTGATc                  <  2:444184/149‑1 (MQ=255)
                                                                                                                              gCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAACACATAAGTGGTGTTCGGGCCACCCAGCGTAGCGTGACCTTTACTCACTGTGATcc                 >  1:293974/1‑149 (MQ=255)
                                                                                                                               cACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAACACATAAGTGGTGTTCGGGCCACCCAGCGTAGCGTGACCTTTACTCACTGTGATCCa                <  3:264616/149‑1 (MQ=255)
                                                                                                                                  tATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAACACATAAgtgg                                                              >  4:105780/1‑100 (MQ=255)
                                                                                                                                  tATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAACACATAAgtgg                                                              <  3:105780/100‑1 (MQ=255)
                                                                                                                                  tATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAACACATAAGTGGTGTTCGGGCCACCCAGCGTAGCGTGACCTTTACTCACTGTGATCCAGtt             >  1:452196/1‑149 (MQ=255)
                                                                                                                                  tATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAACACATAAGTGGTGTTCGGGCCACCCAGCGTAGCGTGACCTTTACTCACTGTGATCCAGtt             <  1:546672/149‑1 (MQ=255)
                                                                                                                                  tATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAACACATAAGTGGTGTTCGGGCCACCCAGCGTAGCGTGACCTTTACTCACTGTGATCCAGtt             >  2:47994/1‑149 (MQ=255)
                                                                                                                                       gtgaattGCCGGATGCATGATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAACACATAAGTGGTGTTCGGGCCACCCAGCGTAGCGTGACCTTTACTCACTGTGATCCAGTTACCtt        >  4:328451/7‑149 (MQ=255)
                                                                                                                                       aCAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAACACATAAGTGGTGTTCGGGCCACCCAGCGTAGCGTGACCTTTACTCACTGTGATCCAGTTACCtt        >  4:465681/1‑149 (MQ=255)
                                                                                                                                            ttGCCGGATGCATGATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAACACATAAGTGGTGTTCGGGCCACCCAGCGTAGCGTGACCTTTACTCACTGTGATCCAGTTACCTTCCGGt   >  4:405025/2‑149 (MQ=255)
                                                                                                                                             tGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAACACATAAGTGGTGTTCGGGCCACCCAGCGTAGCGTGACCTTTACTCACTGTGATCCAGTTACCTTCCGGTa  <  4:198934/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                             
CTGAACTCGCTGTTTTGTTCGGTGGCTTACAGTGTGCTGGAACAGCCGGATGAAGATGAAGGACGGGACGAGTATTAATATTGTGAATCCTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCACTATGAACAAGATGCCGGATCATGCATCCGGCAATTCACTTAAAAACGGTGACCAGCTTCCAGCGGAACCGCTTCTTCACCAGCCTTAAACACATAAGTGGTGTTCGGGCCACCCAGCGTAGCGTGACCTTTACTCACTGTGATCCAGTTACCTTCCGGTA  >  NC_000913/4229285‑4229574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: