Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,285,870:1 +G 9.7% coding (1502/1959 nt) acs ← acetyl‑CoA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,285,8701.G 9.7% 242.2 / 23.5 62coding (1502/1959 nt)acsacetyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base . (28/28);  new base G (2/4);  total (30/32)
Fisher's exact test for biased strand distribution p-value = 6.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.13e-01

TACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTC  >  NC_000913/4285728‑4286018
                                                                                                                                                |                                                                                                                                                    
tactacGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTcgc                                                                                                                                                >  2:39033/1‑149 (MQ=255)
  ctacGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑G‑GCGCGCGTcgcga                                                                                                                                              <  1:111291/149‑3 (MQ=255)
  ctacGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑G‑GCGCGCGTcgcga                                                                                                                                              <  3:398374/149‑3 (MQ=255)
        cggcTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGaaa                                                                                                                                        <  1:568436/149‑1 (MQ=255)
        cggcTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGaaa                                                                                                                                        <  1:39033/149‑1 (MQ=255)
              cGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACata                                                                                                                                  <  3:373610/149‑1 (MQ=255)
               ggCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑G‑GCGCGCGTcgcga                                                                                                                                              <  4:428762/136‑3 (MQ=255)
               ggCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑G‑GCGCGCGTcgcga                                                                                                                                              >  3:428762/1‑134 (MQ=255)
                      ttCGGATGCGCCACCAGCGCCGACTGCATCTCTGCCTTCCCCAGACGGTGACCGGAGACGTTCTGCACGTCGTCGACACGTCCGGTTATCCAGTAATAGCCATCTTCATCGCGTC‑GCGCGC‑CGTTGCCGCTGATATACATATTTTTGaa                                                                                                                          <  4:362618/149‑1 (MQ=255)
                      ttCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGaa                                                                                                                          >  2:387235/1‑149 (MQ=255)
                      ttCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGaa                                                                                                                          <  1:297990/149‑1 (MQ=255)
                         ggATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGt                                                                                                                       <  1:193271/149‑1 (MQ=255)
                         ggATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGCCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGt                                                                                                                       <  2:283736/149‑1 (MQ=255)
                           aTGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTgg                                                                                                                     <  1:292973/149‑1 (MQ=255)
                             gcgcCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGgag                                                                                                                   <  4:86889/149‑1 (MQ=255)
                                 caccaGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGt                                                                                                               >  3:44910/1‑149 (MQ=255)
                                 caccaGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGt                                                                                                               >  1:137117/1‑149 (MQ=255)
                                      gcgcCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTg                                                                                                                      <  1:554026/137‑1 (MQ=255)
                                      gcgcCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTg                                                                                                                      >  2:554026/1‑137 (MQ=255)
                                          cGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGtt                                                                                                      <  3:517300/149‑1 (MQ=255)
                                            aCTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCa                                                                                                    >  2:223665/1‑149 (MQ=255)
                                              tCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTg                                                                                                                      >  4:131958/1‑129 (MQ=255)
                                              tCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTg                                                                                                                      <  3:131958/129‑1 (MQ=255)
                                              tCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCaaa                                                                                                  <  1:241467/149‑1 (MQ=255)
                                                aaTCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGtt                                                                                                      <  3:38852/143‑1 (MQ=255)
                                                aaTCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGtt                                                                                                      >  4:38852/1‑143 (MQ=255)
                                                aaTCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAAc                                                                                                >  4:66219/1‑149 (MQ=255)
                                                aaTCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAAc                                                                                                >  4:427718/1‑149 (MQ=255)
                                                  tctcTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGt                                                                                              <  2:169096/149‑1 (MQ=255)
                                                    tctGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTc                                                                                            <  1:2553/149‑1 (MQ=255)
                                                    tctGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTc                                                                                            <  1:183541/149‑1 (MQ=255)
                                                    tctGCCGTCCCCAGACGGTGACCGGAAACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTc                                                                                            >  4:238431/1‑149 (MQ=255)
                                                          gTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTg                                                                                                                      >  2:498661/1‑117 (MQ=255)
                                                          gTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTg                                                                                                                      <  1:498661/117‑1 (MQ=255)
                                                              ccAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCa                                                                                  >  3:321759/1‑149 (MQ=255)
                                                               cAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCaa                                                                                 <  4:6244/149‑1 (MQ=255)
                                                                aGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCaaa                                                                                >  4:309587/1‑149 (MQ=255)
                                                                    ggTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGc                                                                            >  3:228628/1‑149 (MQ=255)
                                                                                  ttCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑G‑GCGCGCGTcgcga                                                                                                                                              <  2:543641/69‑3 (MQ=38)
                                                                                  ttCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑G‑GCGCGCGTcgcga                                                                                                                                              >  1:543641/1‑67 (MQ=255)
                                                                                           tcgtcCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGa                                                     <  3:529506/149‑1 (MQ=255)
                                                                                                 acacGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGt                                               >  3:235461/1‑149 (MQ=255)
                                                                                                  cacGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTg                                              <  3:427718/149‑1 (MQ=255)
                                                                                                   acGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGa                                             <  3:2632/149‑1 (MQ=255)
                                                                                                       ccGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTAc                                         >  2:555762/1‑149 (MQ=255)
                                                                                                         ggTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCa                                       >  3:437183/1‑149 (MQ=255)
                                                                                                          gTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAg                                      <  4:194881/149‑1 (MQ=255)
                                                                                                                cAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAgg                                                                                                            >  4:197863/1‑73 (MQ=255)
                                                                                                                cAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAgg                                                                                                            <  3:197863/73‑1 (MQ=255)
                                                                                                                 aGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCt                               >  3:267400/1‑149 (MQ=255)
                                                                                                                 aGTAATAGCCATCTTCATCGAGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTCCAAAACCTTCGTGATCGCCAAACAGCGTACGCATCTGACCCGGCCAGGAGTAGGTGATTACCAGGCGACCt                               >  2:428165/1‑149 (MQ=255)
                                                                                                                         ccATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGc                       >  4:176799/1‑149 (MQ=255)
                                                                                                                          cATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGcc                      <  4:327869/149‑1 (MQ=255)
                                                                                                                          cATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGcc                      >  2:39669/1‑149 (MQ=255)
                                                                                                                            tCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGcccc                    >  1:27836/1‑149 (MQ=255)
                                                                                                                              ttCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCt                  >  1:573584/1‑149 (MQ=255)
                                                                                                                               tCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTc                 <  3:161915/149‑1 (MQ=255)
                                                                                                                               tCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTc                 >  4:532664/1‑149 (MQ=255)
                                                                                                                                 gcggcgACGGCGCG‑‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATTGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCa               >  2:38270/4‑149 (MQ=255)
                                                                                                                                 gcggcgACGGCGCG‑‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGt                                               <  1:276529/114‑1 (MQ=255)
                                                                                                                                 gcggcgACGGCGCG‑‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGt                                               >  2:276529/4‑117 (MQ=255)
                                                                                                                                        c‑gcgcgC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTAGAGAAGTAGCTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGCGGCCCCCTCCAGCGGCtt        <  4:44910/149‑1 (MQ=255)
                                                                                                                                               c‑cGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTc  <  4:18225/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
TACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAC‑GCGCGC‑CGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTC  >  NC_000913/4285728‑4286018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑