New junction evidence | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | 4441750 = | 52 (0.800) | 3 (0.050) | 3/248 | NT | 5.8% | coding (427/639 nt) | msrA | methionine sulfoxide reductase A |
? | NC_000913 | 4441769 = | 48 (0.780) | coding (408/639 nt) | msrA | methionine sulfoxide reductase A |
GCAGATATTGCTGGTGGTCATCTTCGGCATAATAAAACGGTGTGGCGTTAGCGATTTCCGTGGTGATGTGACGATCGTCATCGGCGGCAAGCATCGCCGCCTGAAAACGTTCCAGACTGGCGCGAGCTGCGGCATCCTGTTCTGGGGTCAGCGGATAAATCGCTGAACGATACTGCGTGCCGTGGTCATTGCCCTGACGCATTCCCTGGGCGGGATCGTGATTCTCCCAAAATACCTGTAGCAACTGCTCATAGCTGATGACGGAAGGATCGTAAACAATGCGTACCGCTTCGGCA > NC_000913/4441603‑4441898 | tccggtgatacgggtcatgccgaagcggtacgcattgtttacgatccttccgtcatcagctatgagcagttgctacaggtattttgggagaatcacgatcccgcccagggaatgcgtcagggcaatgaccacggcacgcagtatcgtTc > 3:29918‑M1/148‑149 (MQ=255) ccggtgatacgggtcatgccgaagcggtacgcattgtttacgatccttccgtcatcagctatgagcagttgctacaggtattttgggagaatcacgatcccgcccagggaatgcgtcagggcaatgaccacggcacgcagtatcgtTCa > 1:215884‑M1/147‑149 (MQ=255) gATATTGCTGGTGGTCATCTTCGGCATAATAAAACGGTGTGGCGTTAGCGATTTCCGTGGTGATGTGACGATCGTCATCGGCGGCAAGCATCGCCGCCTGAAAACGTTCCAGACTGGCGCGAGCTGCGGCATCCTGTTCTGGGGTCAGc > 4:560820/1‑149 (MQ=255) gATATTGCTGGTGGTCATCTTCGGCATAATAAAACGGTGTGGCGTTAGCGATTTCCGTGGTGATGTGACGATCGTCATCGGCGGCAAGCATCGCCGCCTGAAAACGTTCCAGACTGGCGCGAGCTGCGGCATCCTGTTCTGGGGTCAGc > 4:295400/1‑149 (MQ=255) atTGCTGGTGGTCATCTTCGGCATAATAAAACGGTGTGGCGTTAGCGATTTCCGTGGTGATGTGACGATCGTCATCGGCGGCAAGCATCGCCGCCTGAAAACGTTCCAGACTGGCGCGAGCTGCGGCATCCTGTTCTGGGGTCAGCGGa > 3:292033/1‑149 (MQ=255) ttGCTGGTGGTCATCTTCGGCATAATAAAACGGTGTGGCGTTAGCGATTTCCGTGGTGATGTGACGATCGTCATCGGCGGCAAGCATCGCCGCCTGAAAACGTTCCAGACTGGCGCGAGCTGCGGCATCCTGTTCTGGGGTCAGCGGAt < 3:560820/149‑1 (MQ=255) cTGGTGGTCATCTTCGGCATAATAAAACGGTGTGGCGTTAGCGATTTCCGTGGTGATGTGACGATCGTCATCGGCGGCAAGCATCGCCGCCTGAAAACGTTCCAGACTGGCGCGAGCTGCGGCATCCTGTTCTGGGGTCAGCGGATaaa < 4:78068/149‑1 (MQ=255) ggtCATCTTCGGCATAATAAAACGGTGTGGCGTTAGCGATTTCCGTGGTGATGTGACGATCGTCATCGGCGGCAAGCATCGCCGCCTGAAAACGTTCCAGACTGGCGCGAGCTGCGGCATCCTGTTCTGGGGTCAGCGGATAAATCGCt < 1:521003/149‑1 (MQ=255) tCATCTTCGGCATAATAAAACGGTGTGGCGTTAGCGATTTCCGTGGTGATGTGACGATCGTCATCGGCGGCAAGCATCGCCGCCTGAAAACGTCCCAGACTGGCGCGAGCTGCGGCATCCTGTTCTGGGGTCAGCGGATAAATCGCTGa > 1:330523/1‑149 (MQ=255) gccgaagcggtacgcattgtttacgatccttccgtcatcagctatgagcagttgctacaggtattttgggagaatcacgatcccgcccagggaatgcgtcagggcaatgaccacggcacgcagtatcgtTCAGCGGATAAATCGCTGaa > 4:143622‑M1/130‑149 (MQ=255) aTCTTCGGCATAATAAAACGGTGTGGCGTTAGCGATTTCCGTGGTGATGTGACGATCGTCATCGGCGGCAAGCATCGCCGCCTGAAAACGTTCCAGACTGGCGCGAGCTGCGGCATCCTGTTCTGGGGTCAGCGGATAAATCGCTGAAc > 4:445272/1‑149 (MQ=255) ttCGGCATAATAAAACGGTGTGGCGTTAGCGATTTCCGTGGTGATGTGACGATCGTCATCGGCGGCAAGCATCGCCGCCTGAAAACGTTCCAGACTGGCGCGAGCTGCGGCATCCTGTTCTGGGGTCAGCGGATAAATCGCTGAACGAt > 1:282517/1‑149 (MQ=255) ttCGGCATAATAAAACGGTGTGGCGTTAGCGATTTCCGTGGTGATGTGACGATCGTCATCGGCGGCAAGCATCGCCGCCTGAAAACGTTCCAGACTGGCGCGAGCTGCGGCATCCTGTTCTGGGGTCAGCGGATAAATCGCTGAACGAt < 3:576484/149‑1 (MQ=255) tCGGCATAATAAAACGGTGTGGCGTTAGCGATTTCCGTGGTGATGTGACGATCGTCATCGGCGGCAAGCATCGCCGCCTGAAAACGTTCCAGACTGGCGCGAGCTGCGGCATCCTGTTCTGGGGTCAGCGGATAAATCGCTGAACGATa < 3:510075/149‑1 (MQ=255) gCATAATAAAACGGTGTGGCGTTAGCGATTTCCGTGGTGATGTGACGATCGTCATCGGCGGCAAGCATCGCCGCCTGAAAACGTTCCAGACTGGCGCGAGCTGCGGCATCCTGTTCTGGGGTCAGCGGATAAATCGCTGAACGATACTg > 4:310445/1‑149 (MQ=255) cATAATAAAACGGTGTGGCGTTAGCGATTTCCGTGGTGATGTGACGATCGTCATCGGCGGCAAGCATCGCCGCCTGAAAACGTTCCAGACTGGCGCGAGCTGCGGCATCCTGTTCTGGGGTCAGCGGATAAATCGCTGAACGATACTGc < 3:255907/149‑1 (MQ=255) cATAATAAAACGGTGTGGCGTTAGCGATTTCCGTGGTGATGTGACGATCGTCATCGGCGGCAAGCATCGCCGCCTGAAAACGTTCCAGACTGGCGCGAGCTGCGGCATCCTGTTCTGGGGTCAGCGGATAAATCGCTGAACGATACTGc < 3:266468/149‑1 (MQ=255) taataaAACGGTGTGGCGTTAGCGATTTCCGTGGTGATGTGACGATCGTCATCGGCGGCAAGCATCGCCGCCTGAAAACGTTCCAGACTGGCGCGAGCTGCGGCATCCTGTTCTGGGGTCAGCGGATAAATCGCTGAACGATACTGCGt < 2:267776/149‑1 (MQ=255) taataaAACGGTGTGGCGTTAGCGATTTCCGTGGTGATGTGACGATCGTCATCGGCGGCAAGCATCGCCGCCTGAAAACGTCCCAGACTGGCGCGAGCTGCGGCATCCTGTTCTGGGGTCAGCGGATAAATCGCTGAACGATACTGCGt < 2:330523/149‑1 (MQ=255) ataaAACGGTGTGGCGTTAGCGATTTCCGTGGTGATGTGACGATCGTCATCGGCGGCAAGCATCGCCGCCTGAAAACGTTCCAGACTGGCGCGAGCTGCGGCATCCTGTTCTGGGGTCAGCGGATAAATCGCTGAACGATACTGCGTGc > 2:523522/1‑149 (MQ=255) ataaAACGGTGTGGCGTTAGCGATTTCCGTGGTGATGTGACGATCGTCATCGGCGGCAAGCATCGCCGCCTGAAAACGTTCCAGACTGGCGCGAGCTGCGGCATCCTGTTCTGGGGTCAGCGGATAAATCGCTGAACGATACTGCGTGc < 1:226289/149‑1 (MQ=255) aaaaCGGTGTGGCGTTAGCGATTTCCGTGGTGATGTGACGATCGTCATCGGCGGCAAGCATCGCCGCCTGAAAACGTTCCAGACTGGCGCGAGCTGCGGCATCCTGTTCTGGGGTCAGCGGATAAATCGCTGAACGATACTGCGTGCCg > 1:461067/1‑149 (MQ=255) aGCGATTTCCGTGGTGATGTGACGATCGTCATCGGCGGCAAGCATCGCCGCCTGAAAACGTTCCAGACTGGCGCGAGCTGCGGCATCCTGTTCTGGGGTCAGCGGATAAATCGCTGAACGATACTGCGTGCCGTGGTCATTGCCCTGAc < 4:206741/149‑1 (MQ=255) cGTGGTGATGTGACGATCGTCATCGGCGGCAAGCATCGCCGCCTGAAAACGTTCCAGACTGGCGCGAGCTGCGGCATCCTGTTCTGGGGTCAGCGGATAAATCGCTGAACGATACTGCGTGCCGTGGTCATTGCCCTGACGCATTCCCt < 4:210507/149‑1 (MQ=255) gtggtgATGTGACGATCGTCATCGGCGGCAAGCATCGCCGCCTGAAAACGTTCCAGACTGGCGCGAGCTGCGGCATCCTGTTCTGGGGTCAGCGGATAAATCGCTGAACGATACTGCGTGCCGTGGTCATTGCCCTGACGCATTCCCTg < 1:105178/149‑1 (MQ=255) tggtgATGTGACGATCGTCATCGGCGGCAAGCATCGCCGCCTGAAAACGTTCCAGACTGGCGCGAGCTGCGGCATCCTGTTCTGGGGTCAGCGGATAAATCGCTGAACGATACTGCGTGCCGTGGTCATTGCCCTGACGCATTCCCTgg > 3:400812/1‑149 (MQ=255) tgtgACGATCGTCATCGGCGGCAAGCATCGCCGCCTGAAAACGTTCCAGACTGGCGCGAGCTGCGGCATCCTGTTCTGGGGTCAGCGGATAAATCGCTGAACGATACTGCGTGCCGTGGTCATTGCCCTGACGCATTCCCTGGGCGGGa > 3:335054/1‑149 (MQ=255) aCGATCGTCATCGGCGGCAAGCATCGCCGCCTGAAAACGTTCCAGACTGGCGCGAGCTGCGGCATCCTGTTCTGGGGTCAGCGGATAAATCGCTGAACGATACTGCGTGCCGTGGTCATTGCCCTGACGCATTCCCTGGGCGGGATCGt < 4:455101/149‑1 (MQ=255) gATCGTCATCGGCGGCAAGCATCGCCGCCTGAAAACGTTCCAGACTGGCGCGAGCTGCGGCATCCTGTTCTGGGGTCAGCGGATAAATCGCTGAACGATACTGCGTGCCGTGGTCAtt > 3:87913/1‑118 (MQ=255) gATCGTCATCGGCGGCAAGCATCGCCGCCTGAAAACGTTCCAGACTGGCGCGAGCTGCGGCATCCTGTTCTGGGGTCAGCGGATAAATCGCTGAACGATACTGCGTGCCGTGGTCAtt < 4:87913/118‑1 (MQ=255) gATCGTCATCGGCGGCAAGCATCGCCGCCTGAAAACGTTCCAGACTGGCGCGAGCTGCGGCATCCTGTTCTGGGGTCAGCGGATAAATCGCTGAACGATACTGCGTGCCGTGGTCATTGCCCTGACGCATTCCCTGGGCGGGATCGTGa < 1:271480/149‑1 (MQ=255) gATCGTCATCGGCGGCAAGCATCGCCGCCTGAAAACGTTCCAGACTGGCGCGAGCTGCGGCATCCTGTTCTGGGGTCAGCGGATAAATCGCTGAACGATACTGCGTGCCGTGGTCATTGCCCTGACGCATTCCCTGGGCGGGATCGTGa < 4:165969/149‑1 (MQ=255) gATCGTCATCGGCGGCAAGCATCGCCGCCTGAAAACGTTCCAGACTGGCGCGAGCTGCGGCATCCTGTTCTGGGGTCAGCGGATAAATCGCTGAACGATACTGCGTGCCGTGGTCATTGCCCTGACGCATTCCCTGGGCGGGATCGTGa > 4:159342/1‑149 (MQ=255) gATCGTCATCGGCGGCAAGCATCGCCGCCTGAAAACGTTCCAGACTGGCGCGAGCTGCGGCATCCTGTTCTGGGGTCAGCGGATAAATCGCTGAACGATACTGCGTGCCGTGGTCATTGCCCTGACGCATTCCCTGGGCGGGATCGTGa < 2:26109/149‑1 (MQ=255) tCGTCATCGGCGGCAAGCATCGCCGCCTGAAAACGTTCCAGACTGGCGCGAGCTGCGGCATCCTGTTCTGGGGTCAGCGGATAAATCGCTGAACGATACTGCGTGCCGTGGTCATTGCCCTGACGCATTCCCTGGGCGGGATCGTGAtt < 1:182444/149‑1 (MQ=255) tCATCGGCGGCAAGCATCGCCGCCTGAAAACGTTCCAGACTGGCGCGAGCTGCGGCATCCTGTTCTGGGGTCAGCGGATAAATCGCTGAACGATACTGCGTGCCGTGGTCATTGCCCTGACGCATTCCCTGGGCGGGATCGTGATtctc < 4:382223/149‑1 (MQ=255) tCGGCGGCAAGCATCGCCGCCTGAAAACGTTCCAGACTGGCGCGAGCTGCGGCATCCTGTTCTGGGGTCAGCGGATAAATCGCTGAACGATACTGCGTGCCGTGGTCATTGCCCTGACGCATTCCCTGGGCGGGATCGTGATTCTCCCa > 1:339489/1‑149 (MQ=255) gcAAGCATCGCCGCCTGAAAACGTTCCAGACTGGCGCGAGCTGCGGCATCCTGTTCTGGGGTCAGCGGATAAATCGCTGAACGATACTGCGTGCCGTGGTCATTGCCCTGACGCATTCCCTGGGCGGGATCGTGATTCTCCCAAAATAc > 1:555758/1‑149 (MQ=255) cAAGCATCGCCGCCTGAAAACGTTCCAGACTGGCGCGAGCTGCGGCATCCTGTTCTGGGGTCAGCGGATAAATCGCTGAACGATACTGCGTGCCGTGGTCATTGCCCTGACGCATTCCCTGGGCGGGATCGTGATTCTCCCAAAACAcc < 2:562138/149‑1 (MQ=255) aaGCATCGCCGCCTGAAAACGTTCCAGACTGGCGCGAGCTGCGGCATCCTGTTCTGGGGTCAGCGGATAAATCGCTGAACGATACTGCGTGCCGTGGTCATTGCCCTGACGCATTCCCTGGGCGGGATCGTGATTCTCCCAAAATACCt > 1:353890/1‑149 (MQ=255) tcacgatcccgcccagggaatgcgtcagggcaatgaccacggcacgcagtatcgtTCAGCGGATAAATCGCTGAACGATa > 2:333916‑M1/56‑80 (MQ=255) tcacgatcccgcccagggaatgcgtcagggcaatgaccacggcacgcagtatcgtTCAGCGGATAAATCGCTGAACGATa < 1:333916‑M1/25‑1 (MQ=255) aTCGCCGCCTGAAAACGTTCCAGACTGGCGCGAGCTGCGGCATCCTGTTCTGGGGTCAGCGGATAAATCGCTGAACGATACTGCGTGCCGTGGTCATTGCCCTGACGCATTCCCTGGGCGGGATCGTGATTCTCCCAAAATACCTGTAg < 1:464871/149‑1 (MQ=255) ccgccTGAAAACGTTCCAGACTGGCGCGAGCTGCGGCATCCTGTTCTGAGGTCAGCGGATAAATCGCTGAACGATACTGCGTGACGTGGTCATTGCCCTGACGCATTCCCTGGGCGGGATCGTGATTCTCCCAAAATACCTGTAGCAAc < 2:437796/149‑1 (MQ=255) gccTGAAAACGTTCCAGACTGGCGCGAGCTGCGGCATCCTGTTCTGGGGTCAGCGGATAAATCGCTGAACGATACTGTGTGCCGTGGTCATTGCCCTGACGCATTCCCTGGGCGGGATCGTGATTCTCCCAAAATACCt > 3:397428/1‑139 (MQ=255) gccTGAAAACGTTCCAGACTGGCGCGAGCTGCGGCATCCTGTTCTGGGGTCAGCGGATAAATCGCTGAACGATACTGCGTGCCGTGGTCATTGCCCTGACGCATTCCCTGGGCGGGATCGTGATTCTCCCAAAATACCt < 4:397428/139‑1 (MQ=255) ccTGAAAACGTTCCAGACTGGCGCGAGCTGCGGCATCCTGTTCTGGGGTCAGCGGATAAATCGCTGAACGATACTGCGTGCCGTGGTCATTGCCCTGACGCATTCCCTGGGCGGGATCGTGATTCTCCCAAAATACCTGTAGCAACTGc < 3:443373/149‑1 (MQ=255) cTGAAAACGTTCCAGACTGGCGCGAGCTGCGGCATCCTGTTCTGGGGTCAGCGGATAAATCGCTGAACGATACTGCGTGCCGTGGTCATTGCCCTGACGCATTCCCTGGGCGGGATCGTGATTCTCCCAAAATACCTGTAGCAACTGCt > 3:52647/1‑149 (MQ=255) tGAAAACGTTCCAGACTGGCGCGAGCTGCGGCATCCTGTTCTGGGGTCAGCGGATAAATCGCTGAACGATACTGCGTGCCGTGGTCATTGCCCTGACGCATTCCCTGGGCGGGATCGTGATTCTCCCAAAATACCTGTAGCAACTGCTc > 3:561605/1‑149 (MQ=255) aaaCGTTCCAGACTGGCGCGAGCTGCGGCATCCTGTTCTGGGGTCAGCGGATAAATCGCTGAACGATACTGCGTGCCGTGGTCATTGCCCTGACGCATTCCCTGGGCGGGATCGTGATTCTCCCAAAATACCTGTAGCAACTGCTCATa < 2:9851/149‑1 (MQ=255) gTTCCAGACTGGCGCGAGCTGCGGCATCCTGTTCTGGGGTCAGCGGATAAATCGCTGAACGATACTGCGTGCCGTGGTCATTGCCCTGACGCATTCCCTGGGCGGGATCGTGATTCTCCCAAAATACCTGTAGCAACTGCTCATAGCtg > 3:9984/1‑149 (MQ=255) ttCCAGACTGGCGCGAGCTGCGGCATCCTGTTCTGGGGTCAGCGGATAAATCGCTGAACGATACTGCGTGCCGTGGTCATTGCCCTGACGCATTCCCTGGGCGGGATCGTGATTCTCCCAAAATACCTGTAGCAACTGCTCATAGCtga < 1:578880/149‑1 (MQ=255) ttCCAGACTGGCGCGAGCTGCGGCATCCTGTTCTGGGGTCAGCGGATAAATCGCTGAACGATACTGCGTGCCGTGGTCATTGCCCTGACGCATTCCCTGGGCGGGATCGTGATTCTCCCAAAATACCTGTAGCAACTGCTCATAGCtga < 3:152136/149‑1 (MQ=255) aGACTGGCGCGAGCTGCGGCATCCTGTTCTGGGGTCAGCGGATAAATCGCTGAACGATACTGCGTGCCGTGGTCATTGCCCTGACGCATTcc > 3:34609/1‑92 (MQ=255) aGACTGGCGCGAGCTGCGGCATCCTGTTCTGGGGTCAGCGGATAAATCGCTGAACGATACTGCGTGCCGTGGTCATTGCCCTGACGCATTcc < 4:34609/92‑1 (MQ=255) gtcagggtaatgaccacggcacgcagtatcgtTCAGc < 2:232789‑M1/5‑1 (MQ=255) gtcagggtaatgaccacggcacgcagtatcgtTCAGc > 1:232789‑M1/33‑37 (MQ=255) cgAGCTGCGGCATCCTGTTCTGGGGTCAGCGGATAAATCGCTGAACGATACTGCGTGCCGTGGTCATTGCCCTGACGCATTCCCTGGGCGGGATCGTGATTCTCCCAAAATACCTGTAGCAACTGCTCATAGCTGATGACGGAAGGATc < 4:308711/149‑1 (MQ=255) tGCGGCATCCTGTTCTGGGGTCAGCGGATAAATCGCTGAACGATACTGCGTGCCGTGTTCATTGCCCTGACGCATTCCCTGGGCGGGATCGTGATTCTCCCAAAATACCTGTAGCAACTGCTCATAGCTGATGACGGAAGGATCGTaaa < 3:295400/149‑1 (MQ=255) gCATCCTGTTCTGGGGTCAGCGGATAAATCGCTGAACGATACTGCGTGCCGTGGTCATTGCCCTGACGCATTCCCTGGGCGGGATCGTGATTCTCCCAAAATACCTGTAGCAACTGCTCATAGCTGATGACGGAAGGATCGTAAACAAt < 4:292033/149‑1 (MQ=255) cATCCTGTTCTGGGGTCAGCGGATAAATCGCTGAACGATACTGCGTGCCGTGGTCATTGCCCTGACGCATTCCCTGGGCGGGATCGTGATTCTCCCAAAATACCTGTAGCAACTGCTCATAGCTGATGACGGAAGGATCGTAAACAATg > 4:294902/1‑149 (MQ=255) ggggTCAGCGGATAAATCGCTGAACGATACTGCGTGCCGTGGTCATTGCCCTGACGCATTCCCTGGGCGGGATCGTGATTCTCCCAAAATACCTGTAGCAACTGCTCATAGCTGATGACGGAAGGATCGTAAACAATGCGTACCGCTTc < 4:335054/149‑1 (MQ=255) ggggTCAGCGGATAAATCGCTGAACGATACTGCGTGCCGTGGTCATTGCCCTGACGCATTCCCTGGGCGGGATCGTGATTCTCCCAAAATACCTGTAGCAACTGCTCATAGCTGATGACGGAAGGATCGTAAACAATGCGTACCGCTTc < 2:282517/149‑1 (MQ=255) gggTCAGCGGATAAATCGCTGAACGATACTGCGTGCCGTGGTCATTGCCCTGACGCATTCCCTGGGCGGGATCGTGATTCTCCCAAAATACCTGTAGCAACTGCTCATAGCTGATGACGGAAGGATCGTAAACAATGCGTACCGCTTCg > 3:444837/1‑149 (MQ=255) TCAGCGGATAAATCGCTGAACGATACTGCGTGCCGTGGTCATTGCCCTGACGCATTCCCTGGGCGGGATCGTGATTCTCCCAAAATACCTGTAGCAACTGCTCATAGCTGATGACGGAAGGATCGTAAACAATGCGTACCGCTTCGGCa < 4:422351‑M1/149‑1 (MQ=255) | GCAGATATTGCTGGTGGTCATCTTCGGCATAATAAAACGGTGTGGCGTTAGCGATTTCCGTGGTGATGTGACGATCGTCATCGGCGGCAAGCATCGCCGCCTGAAAACGTTCCAGACTGGCGCGAGCTGCGGCATCCTGTTCTGGGGTCAGCGGATAAATCGCTGAACGATACTGCGTGCCGTGGTCATTGCCCTGACGCATTCCCTGGGCGGGATCGTGATTCTCCCAAAATACCTGTAGCAACTGCTCATAGCTGATGACGGAAGGATCGTAAACAATGCGTACCGCTTCGGCA > NC_000913/4441603‑4441898 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
Reads not counted as support for junction |
read_name Not counted due to insufficient overlap past the breakpoint. |
read_name Not counted due to not crossing MOB target site duplication. |