Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 787,467 C→G 8.2% K43N (AAG→AAC) ‡ gpmA ← phosphoglyceromutase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913787,4670CG 8.2% 252.0 / 11.2 73K43N (AAG→AAC) ‡gpmAphosphoglyceromutase 1
Reads supporting (aligned to +/- strand):  ref base C (37/30);  new base G (3/3);  total (40/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

TGACGTTCGTTCAGTTTCCAGGATTTCTCAACGGGCAGCCATGCCTGATCCAGTTCGTCCAGCACATTCCACAGGGTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCTTTCTCAGACAGATCCACGTCGTACCAACCGGTGAAACGGTTTTCTTTGTTCCACTGACTTTCGCCATGACGAACCAGAACCAGCTTAGTTACAGCCATATACTTACTCCTCAAATCAT  >  NC_000913/787324‑787615
                                                                                                                                               |                                                                                                                                                    
tGACGTTCGTTCAGTTTCCAGGATTTCTCAACGGGCAGCCATGCCTGATCCAGTTCGTCCAGCACATTCCACAGGGTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTAcc                                                                                                                                                 >  4:525688/1‑149 (MQ=255)
      tcgttcAGTTTCCAGGATTTCTCAACGGGCAGCCATGCCTGATCCAGTTCGTCCAGCACATTCCACAGGGTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCtgctgc                                                                                                                                           <  1:492353/149‑1 (MQ=255)
         ttcAGTTTCCAGGATTTCTCAACGGGCAGCCATGCCTGAACCAGTTCGTCCAGCACATTCCACAGGGTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCttt                                                                                                                                        >  3:184632/1‑149 (MQ=255)
          tcAGTTTCCAGGATTTCTCAACGGGCAGCCATGCCTGATCCAGTTCGTCCAGCACATTCCACAGGGTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCtttt                                                                                                                                       <  4:397033/149‑1 (MQ=255)
               ttCCAGGATTTCTCAACGGGCAGCCATGCCTGATCCAGTTCGTCCAGCACATTCCACAGGGTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTc                                                                                                                                  <  1:374710/149‑1 (MQ=255)
                  cAGGATTTCTCAACGGGCAGCCATGCCTGATCCAGTTCGTCCAGCACATTCCACAGGGTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCt                                                                                                                               >  1:129619/1‑149 (MQ=255)
                  cAGGATTTCTCAACGGGCAGCCATGCCTGATCCAGTTCGTCCAGCACATTCCACAGGGTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCt                                                                                                                               >  2:509543/1‑149 (MQ=255)
                   aGGATTTCTCAACGGGCAGCCATGCCTGATCCAGTTCGTCCAGCACATTCCACAGGGTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCtt                                                                                                                              >  2:22522/1‑149 (MQ=255)
                           tcAACGGGCAGCCATGCCTGATCCAGTTCGTCCAGCACATTCCACAGGGTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCttt                                                                                                                      >  4:380867/1‑149 (MQ=255)
                           tcAACGGGCAGCCATGCCTGATCCAGTTCGTCCAGCACATTCCACAGGGTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCttt                                                                                                                      >  4:211499/1‑149 (MQ=255)
                           tcAACGGGCAGCCATGCCTGATCCAGTTCGTCCAGCACATTCCACAGGGTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCttt                                                                                                                      <  2:399037/149‑1 (MQ=255)
                             aaCGGGCAGCCATGCCTGATCCAGTTCGTCCAGCACATTCCACAGGGTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCTTtct                                                                                                                    >  2:268649/1‑149 (MQ=255)
                              aCGGGCAGCCATGCCTGATCCAGTTCGTCCAGCACATTCCACAGGGTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCTTtctc                                                                                                                   <  4:147698/149‑1 (MQ=255)
                                gggCAGCCATGCCTGATCCAGTTCGTCCAGCACATTCCACAGGGTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCTTTCTcag                                                                                                                 >  1:469983/1‑149 (MQ=255)
                                 ggCAGCCATGCCTGATCCAGTTCGTCCAGCACATTCCACAGGGTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCTTTCTcaga                                                                                                                <  2:118798/149‑1 (MQ=255)
                                     gCCATGCCTGATCCAGTTCGTCCAGCACATTCCACAGGGTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCTGTCTgagaaagg                                                                                                            <  2:129619/149‑2 (MQ=255)
                                      ccATGCCTGATCCAGTTCGTCCAGCACATTCCACAGGGTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCTTTCTCAGACAGAt                                                                                                           >  4:497071/1‑149 (MQ=255)
                                        aTGCCTGATCCAGTTCGTCCAGCACATTCCACAGGGTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCTTTCTcaga                                                                                                                <  1:168210/142‑1 (MQ=255)
                                        aTGCCTGATCCAGTTCGTCCAGCACATTCCACAGGGTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCTTTCTcaga                                                                                                                >  2:168210/1‑142 (MQ=255)
                                                  cAGTTCGTCCAGCACATTCCACAGGGTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCTTTCTCAGACAGATCCACGTCGTAcc                                                                                               <  2:469983/149‑1 (MQ=255)
                                                  cAGTTCGTCCAGCACATTCCACAGGGTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCTTTCTCAGACAGATCCACGTCGTAcc                                                                                               >  4:332025/1‑149 (MQ=255)
                                                    gTTCGTCCAGCACATTCCACAGGGTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCTTTCTCAGACAGATCCACGTCGTACCaa                                                                                             >  1:41146/1‑149 (MQ=255)
                                                       cGTCCAGCACATTCCACAGGGTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCTTTCTCAGACAGATCCACGTCGTACCAACCg                                                                                          >  1:402988/1‑149 (MQ=255)
                                                         tCCAGCACATTCCACAGGGTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTTCTTCGCTTACGCCTTTCTCAGACAGATCCACGTCGTACCAACCGGt                                                                                        >  1:174980/1‑149 (MQ=255)
                                                         tCCAGCACATTCCACAGGGTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCTTTCTCAGACAGATCCACGTCGTACCAACCGGt                                                                                        >  2:260039/1‑149 (MQ=255)
                                                          ccAGCACATTCCACAGGGTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCTTTCTCAGACAGATCCACGTCGTACCAACCGGTg                                                                                       <  1:260039/149‑1 (MQ=255)
                                                           cAGCACATTCCACAGGGTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCTTTCTCAGACAGATCCACGTCGTACCAACCGGTGa                                                                                      <  2:271442/149‑1 (MQ=255)
                                                            aGCACATTCCACAGGGTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCTTTCTCAGACAGATCCACGTCGTACCAACCGGTGaa                                                                                     >  1:457553/1‑149 (MQ=255)
                                                             gCACATTCCACAGGGTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCTTTCTCAGACAGATCCACGTCGTACCAACCGGTGata                                                                                    >  4:502624/1‑147 (MQ=255)
                                                             gCACATTCCACAGGGTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCTTTCTCAGACAGATCCACGTCGTACCAACCGGTGaaa                                                                                    >  1:35606/1‑149 (MQ=255)
                                                                 aTTCCACAGGGTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCTTTCTCAGACAGATCCACGTCGTACCAACCGGTGAAACGGt                                                                                <  3:445299/149‑1 (MQ=255)
                                                                 aTTCCACAGGGTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCTTTCTCAGACAGATCCACGTCGTACCAACCGGTGAAACGGt                                                                                >  1:487439/1‑149 (MQ=255)
                                                                  ttCCACAGGGTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCTTTCTCAGACAGATCCACGTCGTACCAACCGGTGAAACGGtt                                                                               >  3:153513/1‑149 (MQ=255)
                                                                      acaGGGTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCTTTCTCAGACAGATCCACGTCGTACCAACCGGTGAAACGGTTTTCt                                                                           >  4:468458/1‑149 (MQ=255)
                                                                      acaGGGTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCTTTCTCAGACAGATCCACGTCGTACCAACCGGTGAAACGGTTTTCt                                                                           <  3:326323/149‑1 (MQ=255)
                                                                      acaGGGTATGGATAGCGCGTTTCACCACAGAAGTGTAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGTCTTTCTCAGACAGAACCACGTCGTACCAACCGGTGAAACGGTTTTCt                                                                           <  4:444856/149‑1 (MQ=255)
                                                                       caGGGTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCTTTCTCAGACAGATCCACGTCGTACCAACCGGTGAAACGGTTTTCtt                                                                          <  3:497071/149‑1 (MQ=255)
                                                                        aGGGTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCTTTCTCAGACAGATCCACGTCGTACCAACCGGTGAAACGGTTTTCttt                                                                         <  1:347381/149‑1 (MQ=255)
                                                                         gggTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCTTTCTCAGACAGATCCACGTCGTACCAACCGGTGAAACGGTTTTCTTTg                                                                        <  4:542400/149‑1 (MQ=255)
                                                                           gTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCTTTCTCAGACAGATCCACGTCGTACCAACCGGTGAAACGGTTTTCTTTGtt                                                                      <  1:566873/149‑1 (MQ=255)
                                                                                  tAGCGCGTTTCAGCACAGAAGTGTAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCTTTCTCAGACAGATCCACGTCGTACCAACCGGTGAAACGGTTTTCTTTGTTCCACTGa                                                               <  4:58834/149‑1 (MQ=255)
                                                                                              gCACAGAAGTGTAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCTTTCTCAGACAGATCCACGTCGTACCAACCGGTGAAACGGTTTTCTTTGTTCCACTGACTTTCGCCATGa                                                   <  2:41146/149‑1 (MQ=255)
                                                                                                acaGAAGTGTAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCTTTCTCAGACAGATCCACGTCGTACCAACCGGTGAAACGGTTTTCTTTGTTCCACTGACTTTCGCCATGACg                                                 >  4:455803/1‑149 (MQ=255)
                                                                                                  aGAAGTGTAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCTTTCTCAGACAGATCCACGTCGTACCAACCGGTGAAACGGTTTTCTTTGTTCCACTGACTTTCGCCATGACgaa                                               >  2:527569/1‑149 (MQ=255)
                                                                                                  aGAAGTGTAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCTTTCTCAGACAGATCCACGTCGTACCAACCGGTGAAACGGTTTTCTTTGTTCCACTGACTTTCGCCATGACgaa                                               >  4:46760/1‑149 (MQ=255)
                                                                                                         tAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCTTTCTCAGACAGATCCACGTCGTACCAACCGGTGAAACGGTTTTCTTTGTTCTACTGACTTTCGCCATGACgaaccagaac                                        <  3:502624/149‑1 (MQ=255)
                                                                                                         tAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCTTTCTCAGACAGATCCACGTCGTACCAACCGGTGAAACGGTTTTCTTTGTTCCACTGACTTTCGCCATGACgaaccagaac                                        >  1:196022/1‑149 (MQ=255)
                                                                                                           aGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCTTTCTCAGACAGATCCACGTCGTACCAACCGGTGAAACGGTTTTCTTTGTTCCACTGACTTTCGCCATGACgaaccagaacca                                      <  3:380867/149‑1 (MQ=255)
                                                                                                            gCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCTTTCTCAGACATATCCACGTCGTACCAACCGGTGAAACGGTTTTCTTTGTTCCACTGACTTTCGCCATGACGaaccagaaccag                                     >  1:427441/1‑149 (MQ=255)
                                                                                                            gCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCTTTCTCAGACAGATCCACGTCGTACCAACCGGTGAAACGGTTTTCTTTGTTCCACTGACTTTCGc                                                        >  4:187924/1‑130 (MQ=255)
                                                                                                            gCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCTTTCTCAGACAGATCCACGTCGTACCAACCGGTGAAACGGTTTTCTTTGTTCCACTGACTTTCGc                                                        <  3:187924/130‑1 (MQ=255)
                                                                                                            gCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCTTTCTCAGACAGATCCACGTCGTACCAACCGGTGAAACGGTTTTCTTTGTTCCACTGACTTTCGCCATGACGaaccagaaccag                                     >  1:552920/1‑149 (MQ=255)
                                                                                                            gCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCTTTCTCAGACAGATCCACGTCGTACCAACCGGTGAAACGGTTTTCTTTGTTCCACTGACTTTCGCCATGACGaaccagaaccag                                     >  1:206884/1‑149 (MQ=255)
                                                                                                             cAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCTTTCTCAGACAGATCCACGTCGTACCAACCGGTGAAACGGTTTTCTTTGTTCCACTGACTTTc                                                          <  1:426186/127‑1 (MQ=255)
                                                                                                             cAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCTTTCTCAGACAGAACCACGTCGTACAAACCGGTGAAACGGTTTTCTTTGTTCCACTGACTTTc                                                          >  2:426186/1‑127 (MQ=255)
                                                                                                              aaaGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCTTTCTCAGACAGATCCACGTCGTACCAACCGGTGAAACGGTTTTCTTTGTTCCACTGACTTTCGCCATGACGAACCAGAACCAGCt                                   >  4:416488/1‑149 (MQ=255)
                                                                                                                aGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCTTTCTCAGACAGATCCACGTCGTACCAACCGGTGAAACGGTTTTCTTTGTTCCACTGACTTTCGCCATGACGAACCAGAACCAGCTTa                                 <  2:552920/149‑1 (MQ=255)
                                                                                                                aGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCTTTCTCAGACAGATCCACGTCGTACCAACCGGTGAAACGGTTTTCTTTGTTCCACTGACTTTCGCCATGACGAACCAGAACCAGCTTa                                 <  2:206884/149‑1 (MQ=255)
                                                                                                                  tCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCTTTCTCAGACAGATCCACGTCGTACCAACCGGTGAAACGGTTTTCTTTGTTCCACTGACTTTCGCCATGACGAACCAGAACCAGCTTAGt                               <  4:11090/149‑1 (MQ=255)
                                                                                                                     aaGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCttt                                                                                                                      <  1:441348/59‑1 (MQ=255)
                                                                                                                     aaGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCttt                                                                                                                      >  2:441348/1‑59 (MQ=255)
                                                                                                                               cTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCTTTCTCAGACAGATCCACGTCGTACCAACCGGTGAAACGGTTTTCTTTGTTCCACTGACTTTCGCCATGACGAACCAGAACCAGCTTAGTTACAGCCATAtac                  >  3:25509/1‑149 (MQ=255)
                                                                                                                               cTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCTTTCTCAGACAGATCCACGTCGTACCAACCGGTGAAACGGTTTTCTTTGTTCCACTGACTTTCGCCATGACGAACCAGAACCAGCTTAGTTACAGCCATAtac                  >  1:491354/1‑149 (MQ=255)
                                                                                                                                ttCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCTTTCTCAGACAGATCCACGTCGTACCAACCGGTGAAACGGTTTTCTTTGTTCCACTGACTTTCGCCATGACGAACCAGAACCAGCTTAGTTACAGCCATAtact                 >  2:201642/1‑149 (MQ=255)
                                                                                                                                     ctTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCTTTCTCAGACAGATCCACGTCGTACCAACCGGTGAAACGGTTTTCTTTGTTCCACTGACTTTCGCCATGACGAACCAGAACCAGCTTAGTTACAGCCATATACTTActc            <  1:134274/149‑1 (MQ=255)
                                                                                                                                     aaagCAGCAGGTAAGCTGCTGCTTTTGCTTCGCTTACGCCTTTCTCAGACAGATCCACGTCGTa                                                                                                 <  3:114333‑M1/60‑1 (MQ=255)
                                                                                                                                     aaagCAGCAGGTAAGCTGCTGCTTTTGCTTCGCTTACGCCTTTCTCAGACAGATCCACGTCGTa                                                                                                 >  4:114333‑M1/5‑64 (MQ=255)
                                                                                                                                     aaagCAGCAGGTAAGCTGCTGCTTTTGCTTCGCTTACGCCTTTCTCAGACAGATCCACGTCGTACCAACCGGTGAAACGGTTTTCt                                                                           <  3:15458‑M1/82‑1 (MQ=255)
                                                                                                                                     aaagCAGCAGGTAAGCTGCTGCTTTTGCTTCGCTTACGCCTTTCTCAGACAGATCCACGTCGTACCAACCGGTGAAACGGTTTTCt                                                                           >  4:15458‑M1/5‑86 (MQ=255)
                                                                                                                                      tttCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCTTTCTCAGACAGATCCACGTCGTAc                                                                                                <  2:246731/64‑1 (MQ=255)
                                                                                                                                      tttCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCTTTCTCAGACAGATCCACGTCGTAc                                                                                                >  1:246731/1‑64 (MQ=255)
                                                                                                                                      aagCAGCAGGTAAGCTGCTGCTTTTGCTTCGCTTACGCCTTTCTCAGACAGATCCACGTCGTACCAATCGGTGAAACGGTTTTCttt                                                                         <  1:193630‑M1/84‑1 (MQ=255)
                                                                                                                                      aagCAGCAGGTAAGCTGCTGCTTTTGCTTCGCTTACGCCTTTCTCAGACAGATCCACGTCGTACCAACCGGTGAAACGGTTTTCttt                                                                         >  2:193630‑M1/4‑87 (MQ=255)
                                                                                                                                               cTTACCTGCTGCTTTTGCTTCGCTTACGCCTTTCTCAGACAGATCCACGTCGTACCAACCGGTGAAACGGTTTTCTTTGTTCCACTGACTTTCGCCATGACGAACCAGAACCAGCTTAGTTACAGCCATATACTTACTCCTCAAatcat  <  2:487439/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
TGACGTTCGTTCAGTTTCCAGGATTTCTCAACGGGCAGCCATGCCTGATCCAGTTCGTCCAGCACATTCCACAGGGTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCTTTCTCAGACAGATCCACGTCGTACCAACCGGTGAAACGGTTTTCTTTGTTCCACTGACTTTCGCCATGACGAACCAGAACCAGCTTAGTTACAGCCATATACTTACTCCTCAAATCAT  >  NC_000913/787324‑787615

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: