Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,015,912 T→A 25.0% intergenic (+30/+40) rmf → / ← fabA ribosome modulation factor/beta‑hydroxydecanoyl thioester dehydrase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,015,9120TA25.0% 93.8 / 35.2 52intergenic (+30/+40)rmf/fabAribosome modulation factor/beta‑hydroxydecanoyl thioester dehydrase
Reads supporting (aligned to +/- strand):  ref base T (22/17);  new base A (6/7);  total (28/24)
Fisher's exact test for biased strand distribution p-value = 5.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.12e-01

TCAGGCCGGCATCGCCGGACGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATC  >  NC_000913/1015765‑1016054
                                                                                                                                                   |                                                                                                                                              
tCAGGCCGGCATCGCCGGACGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCAtt                                                                                                                                               >  3:544136/1‑149 (MQ=255)
        gCATCGCCGGACGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCAATGCGGAGGt                                                                                                                                       <  3:133258/149‑1 (MQ=255)
        gCATCGCCGGACGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCAATGCGGAGGt                                                                                                                                       <  3:566357/149‑1 (MQ=255)
        gCATCGCCGGACGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCAATGCGGAGGt                                                                                                                                       <  4:379880/149‑1 (MQ=255)
           tCGCCGGACGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTc                                                                                                                                    >  1:128168/1‑149 (MQ=255)
             gCCGGACGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCAATGCGGAGGTTTctt                                                                                                                                  <  2:135364/149‑3 (MQ=255)
                 gACGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCttt                                                                                                                              <  3:308747/149‑1 (MQ=255)
                      tCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATa                                                                                                                         <  3:289739/149‑1 (MQ=255)
                        aaaaGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTTGCCTTTTGATatt                                                                                                                       >  4:419403/1‑147 (MQ=255)
                        aaaaGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATAct                                                                                                                       >  3:29528/1‑149 (MQ=255)
                        aaaaGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATAct                                                                                                                       >  3:134216/1‑149 (MQ=255)
                        aaaaGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATAct                                                                                                                       >  1:235678/1‑149 (MQ=255)
                         aaaGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATActc                                                                                                                      >  1:342243/1‑149 (MQ=255)
                          aaGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATActct                                                                                                                     >  1:157727/1‑149 (MQ=255)
                           aGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGACTGGGAGGCTGGCGAGAAGCCATGGAGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATAGCGGAGGATTCGCCTTTTGATACtctg                                                                                                                    >  2:577805/1‑149 (MQ=255)
                             aaaTGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACtctgtc                                                                                                                  <  2:150150/149‑1 (MQ=255)
                                 gtgtCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGAt                                                                                                              <  2:160322/149‑1 (MQ=255)
                                      ccTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATaa                                                                                                         >  3:489396/1‑149 (MQ=255)
                                      ccTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATaa                                                                                                         <  3:251639/149‑1 (MQ=255)
                                        tATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGGAGGAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCAATGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATc                                                                                                       <  2:229161/149‑1 (MQ=255)
                                          tCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAg                                                                                                     >  2:521957/1‑149 (MQ=255)
                                            aGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGaa                                                                                                   >  1:11235/1‑149 (MQ=255)
                                             gACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGa                                                                                                                                          >  2:574388/1‑109 (MQ=255)
                                             gACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGa                                                                                                                                          <  1:574388/109‑1 (MQ=255)
                                             gACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGa                                                                                                               >  4:251381/1‑136 (MQ=255)
                                             gACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGa                                                                                                               <  3:251381/136‑1 (MQ=255)
                                             gACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAg                                                                                                  <  4:544136/149‑1 (MQ=255)
                                                  tGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGa                                                                                             >  4:282163/1‑149 (MQ=255)
                                                      tCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCAATGCGGAGGt                                                                                                                                       >  2:86202/1‑103 (MQ=255)
                                                      tCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCAATGCGGAGGt                                                                                                                                       <  1:86202/103‑1 (MQ=255)
                                                        aaaGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATc                                                                                       >  2:475234/1‑149 (MQ=255)
                                                               acaATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAAc                                                                                >  1:48791/1‑149 (MQ=255)
                                                                caATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACa                                                                               <  1:432947/149‑1 (MQ=255)
                                                                       tGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGAc                                                                        >  2:13402/1‑149 (MQ=255)
                                                                       tGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGAc                                                                        <  3:239998/149‑1 (MQ=255)
                                                                        gggAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACt                                                                       <  2:128168/149‑1 (MQ=255)
                                                                          gAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACttt                                                                     <  4:29528/149‑1 (MQ=255)
                                                                               tGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGt                                                                >  2:326403/1‑149 (MQ=255)
                                                                                   gagaAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCt                                                            >  1:431038/1‑149 (MQ=255)
                                                                                           aTGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGtat                                                    >  4:428300/1‑149 (MQ=255)
                                                                                           aTGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGtat                                                    <  2:402930/149‑1 (MQ=255)
                                                                                              gCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGa                                                 <  4:134216/149‑1 (MQ=255)
                                                                                                                   tGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCa                            <  2:342243/149‑1 (MQ=255)
                                                                                                                          gTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACCTCGcc                     <  3:428300/149‑1 (MQ=255)
                                                                                                                                   aaaaGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAg            <  2:11235/149‑1 (MQ=255)
                                                                                                                                         aaCCTCCGCAATGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCAt      >  3:415885/1‑149 (MQ=255)
                                                                                                                                          aCCTCCGCAATGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCa                                                                   <  1:397769/87‑1 (MQ=255)
                                                                                                                                          aCCTCCGCAATGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCa                                                                   >  2:397769/1‑87 (MQ=255)
                                                                                                                                          aCCTCCGCAATGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATa     >  1:449656/1‑149 (MQ=255)
                                                                                                                                          aCCTCCGCAATGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATa     >  3:304010/1‑149 (MQ=255)
                                                                                                                                          aCCTCCGCAATGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATa     >  2:410649/1‑149 (MQ=255)
                                                                                                                                             tCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATc  >  4:127310/1‑149 (MQ=255)
                                                                                                                                             tCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATc  >  2:231340/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                              
TCAGGCCGGCATCGCCGGACGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATC  >  NC_000913/1015765‑1016054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑