Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,371,933 T→A 6.0% L9I (TTA→ATA)  ycjN → putative ABC sugar transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,371,9330TA 6.0% 218.8 / 5.0 67L9I (TTA→ATA) ycjNputative ABC sugar transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base T (24/38);  new base A (2/2);  total (27/40)
Fisher's exact test for biased strand distribution p-value = 6.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.22e-01

TTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAATTGATTGCCCGTTTTGAAAAA  >  NC_000913/1371786‑1372073
                                                                                                                                                   |                                                                                                                                            
ttCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGtt                                                                                                                                             <  1:129867/149‑1 (MQ=255)
   aGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGATGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATc                                                                                                                                          >  1:451505/1‑149 (MQ=255)
      aTGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGc                                                                                                                                       <  4:449043/149‑1 (MQ=255)
        gTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCAc                                                                                                                                     <  3:182751/149‑1 (MQ=255)
          aaaTATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTg                                                                                                                                   >  3:510508/1‑149 (MQ=255)
           aaTATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGATAACAGCAcgat                                                                                                                                  <  2:532418/149‑4 (MQ=255)
             tatTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGtt                                                                                                                                <  1:147845/149‑1 (MQ=255)
              atTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGttt                                                                                                                               <  2:558250/149‑1 (MQ=255)
                tACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCa                                                                                                                             <  1:435660/149‑1 (MQ=255)
                 aCTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCAt                                                                                                                            <  4:284089/149‑1 (MQ=255)
                 aCTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCAt                                                                                                                            <  2:62741/149‑1 (MQ=255)
                  cTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTACCAGCACTGGTTTCATg                                                                                                                           <  4:225711/149‑1 (MQ=255)
                        tGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATTAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATCATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGTCCt                                                                                                                     >  3:47007/1‑149 (MQ=255)
                          cATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGa                                                                                                                   <  2:250316/149‑1 (MQ=255)
                           aTGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGAt                                                                                                                  >  2:277822/1‑149 (MQ=255)
                              gTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATAAAATAGCGGTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGACCATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATGTc                                                                                                               <  4:47007/149‑1 (MQ=255)
                                     tCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAg                                                                                                                                        <  3:138445/117‑1 (MQ=255)
                                     tCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAg                                                                                                                                        >  4:138445/1‑117 (MQ=255)
                                       tGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCa                                                                                                                                      >  2:11793/1‑117 (MQ=255)
                                       tGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCa                                                                                                                                      <  1:11793/117‑1 (MQ=255)
                                        gATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTaaa                                                                                                     >  1:109769/1‑149 (MQ=255)
                                        gATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTaaa                                                                                                     >  3:82771/1‑149 (MQ=255)
                                           tAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAaagaa                                                                                                  <  4:132062/149‑1 (MQ=255)
                                                aaGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGaaaa                                                                                             <  4:268440/149‑1 (MQ=255)
                                                  gTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATa                                                                                           >  1:422528/1‑149 (MQ=255)
                                                   ttGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATaa                                                                                          <  3:327476/149‑1 (MQ=255)
                                                     gATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATaaaa                                                                                        <  3:537898/149‑1 (MQ=255)
                                                         tatTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGaa                                                                                    <  3:563213/149‑1 (MQ=255)
                                                         tatTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGaa                                                                                    >  3:115148/1‑149 (MQ=255)
                                                          atTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAAt                                                                                   <  2:373801/149‑1 (MQ=255)
                                                               gTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATc                                                                              >  2:569582/1‑149 (MQ=255)
                                                                  aTGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCAt                                                                           <  1:578904/149‑1 (MQ=255)
                                                                   tGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATc                                                                          <  3:559700/149‑1 (MQ=255)
                                                                    gAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAg                                                                                                             <  4:197121/113‑1 (MQ=255)
                                                                    gAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCACGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAg                                                                                                             >  3:197121/1‑113 (MQ=255)
                                                                      aaTAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTAt                                                                               <  3:177610/141‑1 (MQ=255)
                                                                      aaTAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTAt                                                                               >  4:177610/1‑141 (MQ=255)
                                                                                 gcCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGATGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCAttc                                                            >  4:67004/1‑149 (MQ=255)
                                                                                   cccTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAGAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGa                                                                                                                   >  2:288975/1‑92 (MQ=255)
                                                                                   cccTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAGAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGa                                                                                                                   <  1:288975/92‑1 (MQ=255)
                                                                                     cTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTTATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCg                                                        <  4:201701/149‑1 (MQ=255)
                                                                                          aGGTTATGTTGATTAAATAAAAAGGACCAGCACATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGa                                                   <  4:340346/149‑1 (MQ=255)
                                                                                            gTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGc                                                 >  3:165362/1‑149 (MQ=255)
                                                                                                     aTTAAATAAAATGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCTGTGGAGCAGGAGCGcc                                        >  1:443235/1‑149 (MQ=255)
                                                                                                      ttAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGTGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCAttctt                                                          >  4:388170/1‑130 (MQ=255)
                                                                                                      ttAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGTGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCAttctt                                                          <  3:388170/130‑1 (MQ=255)
                                                                                                       taaataaaAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCACCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAg                                      <  2:451505/149‑1 (MQ=255)
                                                                                                           taaaAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCg                                  >  3:335143/1‑149 (MQ=255)
                                                                                                           taaaAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATGCTTCGGTGGAGCAGGAGCGCCAGGCCg                                  >  1:296964/1‑149 (MQ=255)
                                                                                                             aaaaGGAACCTCTCATGATTAAATCAAAAATCGTGCTGTTAGCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTAt                                                                  <  2:91352/115‑1 (MQ=255)
                                                                                                             aaaaGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTAt                                                                  >  1:91352/1‑115 (MQ=255)
                                                                                                               aaGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTAt                              <  2:154853/149‑1 (MQ=255)
                                                                                                                 ggAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCa                            <  2:422528/149‑1 (MQ=255)
                                                                                                                       tctcATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAat                      <  2:590509/149‑1 (MQ=255)
                                                                                                                          cATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAattga                   <  4:218160/149‑1 (MQ=255)
                                                                                                                           aTGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAattgat                  <  1:569582/149‑1 (MQ=255)
                                                                                                                           aTGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAattgat                  <  2:66833/149‑1 (MQ=255)
                                                                                                                               ttAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCa                            >  2:463033/1‑135 (MQ=255)
                                                                                                                               ttAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCa                            <  1:463033/135‑1 (MQ=255)
                                                                                                                                    tCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTAt                                                                  <  3:252329/92‑1 (MQ=255)
                                                                                                                                    tCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTAt                                                                  >  4:252329/1‑92 (MQ=255)
                                                                                                                                    tCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGt                                 >  1:280851/1‑125 (MQ=255)
                                                                                                                                    tCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGt                                 <  2:280851/125‑1 (MQ=255)
                                                                                                                                      aaaaaTCGTGCTGGTATCAGCACTGGCTTTATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAAAGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGCATCGCCAAGCCGTTATCAGTAAATTGATTGCCCGTTTTg       >  2:331673/1‑149 (MQ=255)
                                                                                                                                         accagTGCTGATAACAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAgc                                           >  1:327137/5‑110 (MQ=255)
                                                                                                                                         accagTGCTGATAACAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAgc                                           <  2:327137/106‑1 (MQ=255)
                                                                                                                                          ccagTGCTGATAACAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAATTGATTTCCCGTTTTGaaaa   >  2:346531/4‑149 (MQ=255)
                                                                                                                                           tCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAATTGATTGCCCGTTTTGaaaaa  <  3:231878/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                            
TTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAATTGATTGCCCGTTTTGAAAAA  >  NC_000913/1371786‑1372073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: