Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,371,936 T→A 5.8% S10T (TCA→ACA)  ycjN → putative ABC sugar transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,371,9360TA 5.8% 226.2 / 4.9 70S10T (TCA→ACA) ycjNputative ABC sugar transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base T (25/39);  new base A (2/2);  total (27/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.15e-01

AGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAATTGATTGCCCGTTTTGAAAAAGAAAACCCTG  >  NC_000913/1371789‑1372083
                                                                                                                                                   |                                                                                                                                                   
aGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGATGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATc                                                                                                                                                    >  1:451505/1‑149 (MQ=255)
   aTGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGc                                                                                                                                                 <  4:449043/149‑1 (MQ=255)
     gTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCAc                                                                                                                                               <  3:182751/149‑1 (MQ=255)
       aaaTATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTg                                                                                                                                             >  3:510508/1‑149 (MQ=255)
        aaTATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGATAACAGCAcgat                                                                                                                                            <  2:532418/149‑4 (MQ=255)
          tatTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGtt                                                                                                                                          <  1:147845/149‑1 (MQ=255)
           atTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGttt                                                                                                                                         <  2:558250/149‑1 (MQ=255)
             tACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCa                                                                                                                                       <  1:435660/149‑1 (MQ=255)
              aCTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCAt                                                                                                                                      <  2:62741/149‑1 (MQ=255)
              aCTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCAt                                                                                                                                      <  4:284089/149‑1 (MQ=255)
               cTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTACCAGCACTGGTTTCATg                                                                                                                                     <  4:225711/149‑1 (MQ=255)
                     tGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATTAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATCATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGTCCt                                                                                                                               >  3:47007/1‑149 (MQ=255)
                       cATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGa                                                                                                                             <  2:250316/149‑1 (MQ=255)
                        aTGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGAt                                                                                                                            >  2:277822/1‑149 (MQ=255)
                           gTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATAAAATAGCGGTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGACCATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATGTc                                                                                                                         <  4:47007/149‑1 (MQ=255)
                                  tCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAg                                                                                                                                                  <  3:138445/117‑1 (MQ=255)
                                  tCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAg                                                                                                                                                  >  4:138445/1‑117 (MQ=255)
                                    tGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCa                                                                                                                                                <  1:11793/117‑1 (MQ=255)
                                    tGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCa                                                                                                                                                >  2:11793/1‑117 (MQ=255)
                                     gATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTaaa                                                                                                               >  1:109769/1‑149 (MQ=255)
                                     gATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTaaa                                                                                                               >  3:82771/1‑149 (MQ=255)
                                        tAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAaagaa                                                                                                            <  4:132062/149‑1 (MQ=255)
                                             aaGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGaaaa                                                                                                       <  4:268440/149‑1 (MQ=255)
                                               gTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATa                                                                                                     >  1:422528/1‑149 (MQ=255)
                                                ttGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATaa                                                                                                    <  3:327476/149‑1 (MQ=255)
                                                  gATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATaaaa                                                                                                  <  3:537898/149‑1 (MQ=255)
                                                      tatTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGaa                                                                                              >  3:115148/1‑149 (MQ=255)
                                                      tatTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGaa                                                                                              <  3:563213/149‑1 (MQ=255)
                                                       atTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAAt                                                                                             <  2:373801/149‑1 (MQ=255)
                                                            gTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATc                                                                                        >  2:569582/1‑149 (MQ=255)
                                                               aTGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCAt                                                                                     <  1:578904/149‑1 (MQ=255)
                                                                tGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATc                                                                                    <  3:559700/149‑1 (MQ=255)
                                                                 gAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAg                                                                                                                       <  4:197121/113‑1 (MQ=255)
                                                                 gAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCACGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAg                                                                                                                       >  3:197121/1‑113 (MQ=255)
                                                                   aaTAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTAt                                                                                         >  4:177610/1‑141 (MQ=255)
                                                                   aaTAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTAt                                                                                         <  3:177610/141‑1 (MQ=255)
                                                                              gcCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGATGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCAttc                                                                      >  4:67004/1‑149 (MQ=255)
                                                                                cccTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAGAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGa                                                                                                                             >  2:288975/1‑92 (MQ=255)
                                                                                cccTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAGAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGa                                                                                                                             <  1:288975/92‑1 (MQ=255)
                                                                                  cTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTTATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCg                                                                  <  4:201701/149‑1 (MQ=255)
                                                                                       aGGTTATGTTGATTAAATAAAAAGGACCAGCACATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGa                                                             <  4:340346/149‑1 (MQ=255)
                                                                                         gTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGc                                                           >  3:165362/1‑149 (MQ=255)
                                                                                                  aTTAAATAAAATGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCTGTGGAGCAGGAGCGcc                                                  >  1:443235/1‑149 (MQ=255)
                                                                                                   ttAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGTGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCAttctt                                                                    <  3:388170/130‑1 (MQ=255)
                                                                                                   ttAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGTGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCAttctt                                                                    >  4:388170/1‑130 (MQ=255)
                                                                                                    taaataaaAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCACCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAg                                                <  2:451505/149‑1 (MQ=255)
                                                                                                        taaaAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCg                                            >  3:335143/1‑149 (MQ=255)
                                                                                                        taaaAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATGCTTCGGTGGAGCAGGAGCGCCAGGCCg                                            >  1:296964/1‑149 (MQ=255)
                                                                                                          aaaaGGAACCTCTCATGATTAAATCAAAAATCGTGCTGTTAGCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTAt                                                                            <  2:91352/115‑1 (MQ=255)
                                                                                                          aaaaGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTAt                                                                            >  1:91352/1‑115 (MQ=255)
                                                                                                            aaGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTAt                                        <  2:154853/149‑1 (MQ=255)
                                                                                                              ggAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCa                                      <  2:422528/149‑1 (MQ=255)
                                                                                                                    tctcATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAat                                <  2:590509/149‑1 (MQ=255)
                                                                                                                       cATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAattga                             <  4:218160/149‑1 (MQ=255)
                                                                                                                        aTGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAattgat                            <  2:66833/149‑1 (MQ=255)
                                                                                                                        aTGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAattgat                            <  1:569582/149‑1 (MQ=255)
                                                                                                                            ttAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCa                                      <  1:463033/135‑1 (MQ=255)
                                                                                                                            ttAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCa                                      >  2:463033/1‑135 (MQ=255)
                                                                                                                                 tCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTAt                                                                            <  3:252329/92‑1 (MQ=255)
                                                                                                                                 tCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTAt                                                                            >  4:252329/1‑92 (MQ=255)
                                                                                                                                 tCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGt                                           <  2:280851/125‑1 (MQ=255)
                                                                                                                                 tCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGt                                           >  1:280851/1‑125 (MQ=255)
                                                                                                                                   aaaaaTCGTGCTGGTATCAGCACTGGCTTTATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAAAGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGCATCGCCAAGCCGTTATCAGTAAATTGATTGCCCGTTTTg                 >  2:331673/1‑149 (MQ=255)
                                                                                                                                      accagTGCTGATAACAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAgc                                                     <  2:327137/106‑1 (MQ=255)
                                                                                                                                      accagTGCTGATAACAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAgc                                                     >  1:327137/5‑110 (MQ=255)
                                                                                                                                       ccagTGCTGATAACAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAATTGATTTCCCGTTTTGaaaa             >  2:346531/4‑149 (MQ=255)
                                                                                                                                        tCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAATTGATTGCCCGTTTTGaaaaa            <  3:231878/149‑1 (MQ=255)
                                                                                                                                                 tATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAATTGATTGCCCGTTTTGAAAAAGAAAACCCt   <  1:277822/149‑1 (MQ=255)
                                                                                                                                                 tATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAATTGATTGCCCGTTTTGAAAAAGAAAACCCt   <  4:82771/149‑1 (MQ=255)
                                                                                                                                                  aTCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAATTGATTGCCCGTTTTGAAAAAGAAAACCCTg  <  4:510508/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
AGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAATTGATTGCCCGTTTTGAAAAAGAAAACCCTG  >  NC_000913/1371789‑1372083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: