Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,672,481 A→T 6.5% A174A (GCA→GCT ydgD → putative peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,672,4810AT 6.5% 208.6 / 4.9 62A174A (GCA→GCTydgDputative peptidase
Reads supporting (aligned to +/- strand):  ref base A (19/39);  new base T (2/2);  total (21/41)
Fisher's exact test for biased strand distribution p-value = 5.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.97e-01

TTAAAAGCAGATGGGGATGGTTGGATTGTACCTCCCGCAGCCGCGCCGTGGGACTTCGGATTGATTGTGCTACGTAATCCCCCTTCTGGCATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAG  >  NC_000913/1672341‑1672627
                                                                                                                                            |                                                                                                                                                  
ttAAAAGCAGATGGGGATGGTTGGATTGTACCTCCCGCAGCCGCGCCGTGGGACTTCGGATTGATTGTGCTACGTAATCCCCCTTCTGGCATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGc                                                                                                                                            <  2:567916/149‑1 (MQ=255)
  aaaaGCAGATGGGGATGGTTGGATTGTACCTCCCGCAGCCGCGCCGTGGGACTTCGGATTGATTGTGCTACGTAATCCCCCTTCTGGCATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCgg                                                                                                                                          <  2:186801/149‑1 (MQ=255)
      gCAGATGGGGATGGTTGGATTGTACCTCCCGCAGCCGCGCCGTGGGACTTCGGATTGATTGTGCTACGTAATCCCCCTTCTGGCATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAgg                                                                                                                                      <  2:2567/149‑1 (MQ=255)
        aGATGGGGATGGTTGGATTGTACCTCCCGCAGCCGCGCCGTGGGACTTCGGATTGATTGTGCTACGTAATCCCCCTTCTGGCATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTc                                                                                                                                    <  3:301782/149‑1 (MQ=255)
               gATGGTTGGATTGTACCTCCCGCAGCCGCGCCGTGGGACTTCGGATTGATTGTGCTACGTAATCCCCCTTCTGGCATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGt                                                                                                                             <  1:256181/149‑1 (MQ=255)
               gATGGTTGGATTGTACCTCCCGCAGCCGCGCCGTGGGACTTCGGATTGATTGTGCTACGTAATCCCCCTTCTGGCATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGt                                                                                                                             <  3:434713/149‑1 (MQ=255)
                 tGGTTGGATTGTACCTCCCGCAGCCGCGCCGTGGGACTTCGGATTGATTGTGCTCCGTAATCCCCCTTCTGGCATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGa                                                                                                                           <  1:102867/149‑1 (MQ=255)
                    ttGGATTGTACCTCCCGCAGCCGCGCCGTGGGACTTCGGATTGATTGTGCTACGTAATCCCCCTTCTGGCATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTc                                                                                                                        >  3:322374/1‑149 (MQ=255)
                    ttGGATTGTACCTCCCGCAGCCGCGCCGTGGGACTTCGGATTGATTGTGCTACGTAATCCCCCTTCTGGCATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTc                                                                                                                        >  3:233187/1‑149 (MQ=255)
                     tGGATTGTACCTCCCGCAGCCGCGCCGTGGGACTTCGGATTGATTGTGCTACGTAATCCCCCTTCTGGCATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTca                                                                                                                       <  4:41929/149‑1 (MQ=255)
                       gATTGTACCTCCCGCAGCCGCGCCGTGGGACTTCGGATTGATTGTGCTACGTAATCCCCCTTCTGGCATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTcagg                                                                                                                     <  4:241767/149‑1 (MQ=255)
                            tACCTCCCGCAGCCGCGCCGTGGGACTTCGGATTGATTGTGCTACGTAATCCCCCTTCTGGCATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCaggcaggc                                                                                                                <  4:41331/149‑1 (MQ=255)
                            tACCTCCCGCAGCCGCGCCGTGGGACTTCGGATTGATTGTGCTACGTAATCCCCCTTCTGGCATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCaggcaggc                                                                                                                >  4:444900/1‑149 (MQ=255)
                                  ccGCAGCCGCGCCGTGGGACTTCGGATTGATTGTGCTACGTAATCCCCCTTCTGGCATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCt                                                                                                          >  2:118781/1‑149 (MQ=255)
                                   cGCAGCCGCGCCGTGGGACTTCGGATTGATTGTGCTACGTAATCCCCCTTCTGGCATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTg                                                                                                         >  3:556841/1‑149 (MQ=255)
                                    gCAGCCGCGCCGTGGGACTTCGGATTGATTGTGCTACGTAATCCCCCTTCTGGCATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGa                                                                                                        <  3:586553/149‑1 (MQ=255)
                                     cAGCCGCGCCGTGGGACTTCGGATTGATTGTGCTACGTAATCCCCCTTCTGGCATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGaa                                                                                                       <  3:444905/149‑1 (MQ=255)
                                     cAGCCGCGCCGTGGGACTTCGGATTGATTGTGCTACGTAATCCCCCTTCTGGCATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGaa                                                                                                       <  1:525073/149‑1 (MQ=255)
                                     cAGCCGCGCCGTGGGACTTCGGATTGATTGTGCTACGTAATCCCCCTTCTGGCATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGaa                                                                                                       <  3:235503/149‑1 (MQ=255)
                                     cAGCCGCGCCGTGGGACTTCGGATTGATTGTGCTACGTAATCCCCCTTCTGGCATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCCACCCTGaa                                                                                                       <  3:539551/149‑1 (MQ=255)
                                        ccGCGCCGTGGGACTTCGGATTGATTGTGCTACGTAATCCCCCTTCTGGCATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAGGTGACTCAGGCAGGCTACCCTGAAGat                                                                                                    <  3:546492/149‑1 (MQ=255)
                                        ccGCGCCGTGGGACTTCGGATTGATTGTGCTACGTAATCCCCCTTCTGGCATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGat                                                                                                    <  2:326801/149‑1 (MQ=255)
                                                tGGGACTTCGGATTGATTGTGCTACGTAATCCCCCTTCTGGCATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGa                                                                                            <  1:118781/149‑1 (MQ=255)
                                                     cTTCGGATTGATTGTGCTACGTAATCCCCCTTCTGGCATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGt                                                                                       <  3:24599/149‑1 (MQ=255)
                                                     cTTCGGATTGATTGTGCTACGTAATCCCCCTTCTGGCATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGt                                                                                       <  2:537888/149‑1 (MQ=255)
                                                      ttCGGATTGATTGTGCTACGTAATCCCCCTTCTGGCATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGtt                                                                                      <  4:302329/149‑1 (MQ=255)
                                                              gattgTGCTACGTAATCCCCCTTCTGGCATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAACGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGtc                                                                              <  1:542040/149‑1 (MQ=255)
                                                               attgTGCTACGTAATCCCCCTTCTGGCATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGtca                                                                             >  1:185142/1‑149 (MQ=255)
                                                                      tACGTAATCCCCCTTCTGGCATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGTCATCAAAAc                                                                      >  1:226282/1‑149 (MQ=255)
                                                                        cGTAATCCCCCTTCTGGCATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGTCATCAAAACtg                                                                    >  4:203481/1‑149 (MQ=255)
                                                                           aaTCCCCCTTCTGGCATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGTCATCAAAACtgtg                                                                  <  3:176767/148‑1 (MQ=255)
                                                                           aaTCCCCCTTCTGGCATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGTCATCAAAACtgtg                                                                  >  4:176767/1‑148 (MQ=255)
                                                                            aTCCCCCTTCTGGCATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGTCATCAAAACTGTGaa                                                                <  3:203481/149‑1 (MQ=255)
                                                                            aTCCCCCTTCTGGCATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGTCATCAAAACTGTGaa                                                                <  1:411693/149‑1 (MQ=255)
                                                                             tCCCCCTTCTGGCATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTaaa                                                                                                                               <  4:163958/85‑1 (MQ=255)
                                                                             tCCCCCTTCTGGCATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTaaa                                                                                                                               >  3:163958/1‑85 (MQ=255)
                                                                                          aTTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAg                                                                                                                                             >  3:331861/1‑58 (MQ=255)
                                                                                          aTTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAg                                                                                                                                             <  4:331861/58‑1 (MQ=255)
                                                                                           ttACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGTCATCAAAACTGTGAAGTGACTGGCTGGgcg                                                 >  1:150439/1‑149 (MQ=255)
                                                                                               gccgttgccgttATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCaaa                                             <  1:297835/149‑1 (MQ=255)
                                                                                               gccgttgccgttATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCaaa                                             <  4:302347/149‑1 (MQ=255)
                                                                                                     gccgttATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCgg                                       <  4:322374/149‑1 (MQ=255)
                                                                                                      ccgttATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGt                                      >  1:297505/1‑149 (MQ=255)
                                                                                                      ccgttATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCGGAAGAGCATCTCGATACGTTGTACAGTCATCAAAACTGTGAAGTGACTGGCTTGGCGCAAACGGCGGt                                      <  2:413696/149‑1 (MQ=255)
                                                                                                         ttATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGAt                                   >  3:147347/1‑149 (MQ=255)
                                                                                                           aTTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGt                                 <  3:444900/149‑1 (MQ=255)
                                                                                                           aTTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGt                                 <  2:150439/149‑1 (MQ=255)
                                                                                                            tttGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTc                                >  3:479407/1‑149 (MQ=255)
                                                                                                                  ggAGATAAATCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGAGTCAGGCAGGCTACCCTTAAGATCATCACGATACTCTGTACAGTCATCAAAACTGTTAAGTGACTGCCTGGGCGCAAACGTCGGTGATATCACATCa                          >  2:131104/1‑149 (MQ=255)
                                                                                                                     gaTAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTg                       >  1:256541/1‑149 (MQ=255)
                                                                                                                      aTAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGc                      <  2:256541/149‑1 (MQ=255)
                                                                                                                         aaGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGAt                   <  4:513329/149‑1 (MQ=255)
                                                                                                                          aGCCGCGCTTACCGCCGCATTATAAGCGGCAGGTCGTAGAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATa                  <  1:364149/149‑1 (MQ=255)
                                                                                                                            ccGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATAcc                >  3:227217/1‑149 (MQ=255)
                                                                                                                                  gacctgccgcTTTTAATGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGTCATCAAAACTGTGAAGTGAc                                                           >  3:512232/6‑100 (MQ=255)
                                                                                                                                  gacctgccgcTTTTAATGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGTCATCAAAACTGTGAAGTGAc                                                           <  4:512232/95‑1 (MQ=255)
                                                                                                                                     ctgccgcTTTTAATGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCaaa                                             <  4:23810/109‑1 (MQ=255)
                                                                                                                                     ctgccgcTTTTAATGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCaaa                                             >  3:23810/3‑111 (MQ=255)
                                                                                                                                     ccgccgCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGAGATGTCACATCAGTGCGATACCTTGCCGGGt       <  4:233187/149‑1 (MQ=255)
                                                                                                                                       gccgcATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGa     >  1:32509/1‑149 (MQ=255)
                                                                                                                                       gccgcATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGa     <  2:383262/149‑1 (MQ=255)
                                                                                                                                          gcATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAg  <  4:296646/149‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                                  
TTAAAAGCAGATGGGGATGGTTGGATTGTACCTCCCGCAGCCGCGCCGTGGGACTTCGGATTGATTGTGCTACGTAATCCCCCTTCTGGCATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAG  >  NC_000913/1672341‑1672627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: