Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,715,919 T→G 5.3% intergenic (‑30/+29) pdxY ← / ← tyrS pyridoxamine kinase/tyrosyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,715,9190TG 5.3% 257.2 / 6.6 76intergenic (‑30/+29)pdxY/tyrSpyridoxamine kinase/tyrosyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base T (42/30);  new base G (2/2);  total (44/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.61e-01

TTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGATCTTCTTCTTTAAAGAAGTATTCAGGATCGGACTGTTTTTCACCGTTAAT  >  NC_000913/1715780‑1716067
                                                                                                                                           |                                                                                                                                                    
ttCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCa                                                                                                                                                >  2:247404/1‑146 (MQ=255)
ttCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCa                                                                                                                                                <  1:247404/146‑1 (MQ=255)
ttCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTc                                                                                                                                             >  4:492432/1‑149 (MQ=255)
     cGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGAGAAATCACTCCCCCt                                                                                                                                        <  2:365374/149‑1 (MQ=255)
         cAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTcc                                                                                                                                    <  4:76805/149‑1 (MQ=255)
          aGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCa                                                                                                                                   >  4:209879/1‑149 (MQ=255)
          aGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCa                                                                                                                                   >  4:198947/1‑149 (MQ=255)
          aGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGAGAAATCACTCCCCCt                                                                                                                                        >  4:323247/1‑144 (MQ=255)
          aGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGAGAAATCACTCCCCCt                                                                                                                                        <  3:323247/144‑1 (MQ=255)
           gACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCAc                                                                                                                                  >  3:113150/1‑149 (MQ=255)
                          gcggcgCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTc                                                                                                                   <  4:16585/149‑1 (MQ=255)
                          gcggcgCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTc                                                                                                                   <  3:334695/149‑1 (MQ=255)
                                 aTCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCaaa                                                                                                            <  1:166523/149‑1 (MQ=255)
                                 aTCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCaaa                                                                                                            >  2:575913/1‑149 (MQ=255)
                                  tCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAAt                                                                                                           <  1:437046/149‑1 (MQ=255)
                                    ggAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCa                                                                                                         >  2:580734/1‑149 (MQ=255)
                                     gAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAg                                                                                                        >  1:356352/1‑149 (MQ=255)
                                      aaaCTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGa                                                                                                       <  3:497127/149‑1 (MQ=255)
                                             gCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCa                                                                                                                                                >  2:130830/1‑101 (MQ=255)
                                             gCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCa                                                                                                                                                <  1:130830/101‑1 (MQ=255)
                                                gCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCa                                                                                                         >  4:249774/1‑137 (MQ=255)
                                                gCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCa                                                                                                         <  3:249774/137‑1 (MQ=255)
                                                 cACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCATGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCACACAGTAATTCtt                                                                                            <  4:367118/149‑1 (MQ=255)
                                                  aCTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCttt                                                                                           <  4:36236/149‑1 (MQ=255)
                                                  aCTGTTACCCGCATGACCATAAACAACGTGAGACTAGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCttt                                                                                           <  2:337252/149‑1 (MQ=255)
                                                   cTGTTACCCGCATGACCATAAACAACGTGAAGATTGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCtttt                                                                                          <  1:161738/149‑1 (MQ=255)
                                                     gTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTa                                                                                        >  1:586335/1‑149 (MQ=255)
                                                     gTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTa                                                                                        >  1:109057/1‑149 (MQ=255)
                                                      ttACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGa                                                                                                       >  1:359018/1‑133 (MQ=255)
                                                      ttACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGa                                                                                                       <  2:359018/133‑1 (MQ=255)
                                                         cccGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACcgc                                                                                    >  4:278027/1‑149 (MQ=255)
                                                         cccGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACcgc                                                                                    >  2:202347/1‑149 (MQ=255)
                                                            gCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGAc                                                                                 <  2:457814/149‑1 (MQ=255)
                                                                   cATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTaa                                                                          >  3:528375/1‑149 (MQ=255)
                                                                   cATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTaa                                                                          >  4:301948/1‑149 (MQ=255)
                                                                    aTAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAg                                                                         >  2:546553/1‑149 (MQ=255)
                                                                    aTAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAg                                                                         >  1:59266/1‑149 (MQ=255)
                                                                       aacaacGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTa                                                                      >  3:1141/1‑149 (MQ=255)
                                                                        acaacGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTaa                                                                     >  2:578724/1‑149 (MQ=255)
                                                                          aacGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTAcc                                                                                      <  4:458236/130‑1 (MQ=255)
                                                                          aacGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTAcc                                                                                      >  3:458236/1‑130 (MQ=255)
                                                                              tGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGAc                                                               <  3:455787/149‑1 (MQ=255)
                                                                                  aCTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCaaa                                                           <  3:209879/149‑1 (MQ=255)
                                                                                      gATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGt                                                                            >  2:281904/1‑128 (MQ=255)
                                                                                      gATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGt                                                                            <  1:281904/128‑1 (MQ=255)
                                                                                            gagaATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGAtct                                                 >  2:410601/1‑149 (MQ=255)
                                                                                             agaATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGAtctt                                                >  1:181666/1‑149 (MQ=255)
                                                                                               aaTATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGAtcttct                                              >  2:508898/1‑149 (MQ=255)
                                                                                                atatTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGAtcttctt                                             <  1:575913/149‑1 (MQ=255)
                                                                                                 tatTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGAtcttcttc                                            >  4:537728/1‑149 (MQ=255)
                                                                                                 tatTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGAtcttcttc                                            >  1:542478/1‑149 (MQ=255)
                                                                                                 tatTTTTCATCATGTTCCCTGCATAAAAACCAGGGGGAGTTTTTTCGTACTCCCCCTGTCCACTTAATGCACTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGAtcttcttc                                            >  3:550015/1‑149 (MQ=255)
                                                                                                  atTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGAtcttcttct                                           <  1:546553/149‑1 (MQ=255)
                                                                                                         atcatGTTCCCTGTATAGAAACCAGGGGGAGTGATTTCTCACTCCCCCTGTCCACTTAATGCATTATTTACAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAATGACCAAACAGACGATCTTATTCTTTAaaga                                    >  4:361679/1‑149 (MQ=255)
                                                                                                         atcatGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGATCTTCTTCTTTAaaga                                    >  4:220506/1‑149 (MQ=255)
                                                                                                          tcacGTTCCCTGTATAAAAACCAGGAGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCGATATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGATCTTCTTCTTTAaagaa                                   >  4:371423/1‑149 (MQ=255)
                                                                                                             tGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGt                                                                       >  1:86551/1‑110 (MQ=255)
                                                                                                             tGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGt                                                                       <  2:86551/110‑1 (MQ=255)
                                                                                                                 cccTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGATCTTCTTCTTTAAAGAAGTATTCa                            <  2:542478/149‑1 (MQ=255)
                                                                                                                   cTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGATCTTCTTCTTTAAAGAAGTATTCAgg                          >  1:198003/1‑149 (MQ=255)
                                                                                                                   cTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGATCTTCTTCTTTAAAGAAGTATTCAgg                          >  1:203907/1‑149 (MQ=255)
                                                                                                                    tGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGATCTTCTTCTTTAAAGAAGTATTCAGGa                         <  2:181666/149‑1 (MQ=255)
                                                                                                                       ataAAAACCAGGGGGAGTGATTTCTCACTCCACCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGATCTTCTTCTTTAAAGAAGTATTCAGGATCg                      <  1:578724/149‑1 (MQ=255)
                                                                                                                        taAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGATCTTCTTCTTTAaagaa                                   >  2:442159/1‑135 (MQ=255)
                                                                                                                        taAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGATCTTCTTCTTTAaagaa                                   <  1:442159/135‑1 (MQ=255)
                                                                                                                         aaaaaCCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGATCTTCTTCTTTAAAGAAGTATTCAGGATCGGa                    >  1:59836/1‑149 (MQ=255)
                                                                                                                         aaaaaCCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGATCTTCTTCTTTAAAGAAGTATTCAGGATCGGa                    >  3:453534/1‑149 (MQ=255)
                                                                                                                              aaaGGGGGAGTGAGAAATCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGATCTTCTTCTTTAAAGAAGTATTCAGGATCGGACTGtt               >  4:387235/3‑149 (MQ=255)
                                                                                                                                 gggggAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGATCTTCTTCTTTAAAGAAGTATTCAGGATCGGACTGttttt            <  3:492432/149‑1 (MQ=255)
                                                                                                                                  ggggAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGAtcttcttc                                            <  1:499812/116‑1 (MQ=255)
                                                                                                                                  ggggAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGAtcttcttc                                            >  2:499812/1‑116 (MQ=255)
                                                                                                                                       gTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGATCTTCTTCTTTAAAGAAGTATTCAGGATCGGACTGTTTTTCACCGt      >  2:256463/1‑149 (MQ=255)
                                                                                                                                        tGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGAtcttctt                                             <  4:26414/109‑1 (MQ=255)
                                                                                                                                        tGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGAtcttctt                                             >  3:26414/1‑109 (MQ=255)
                                                                                                                                        tGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGATCTTCTTCTTTAAAGAAGTATTCAGGATCGGACTGTTTTTCACCGtt     >  4:230001/1‑149 (MQ=255)
                                                                                                                                          aTTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGATCTTCTTCTTTAAAGAAGTATTCAGGATCGGACTGTTTTTCACCGTTaa   <  2:203637/149‑1 (MQ=255)
                                                                                                                                           tttCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGATCTTCTTCTTTAAAGAAGTATTCAGGATCGGACTGTTTTTCACCGTTAAt  >  1:215128/1‑149 (MQ=255)
                                                                                                                                           tttCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGATCTTCTTCTTTAAAGAAGTATTCAGGATCGGACTGTTTTTCACCGTTAAt  >  4:542591/1‑149 (MQ=255)
                                                                                                                                           tttCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGATCTTCTTCTTTAAAGAAGTATTCAGGATCGGACTGTTTTTCACCGTTAAt  >  4:99658/1‑149 (MQ=255)
                                                                                                                                           |                                                                                                                                                    
TTCAGCGGCCAGACGTTCGCGCCCAGGCGGCGCATCGGAAACTCTGCCGCACTGTTACCCGCATGACCATAAACAACGTGAGACTGGATAGCGAGAATATTTTTCATCATGTTCCCTGTATAAAAACCAGGGGGAGTGATTTCTCACTCCCCCTTTCCACTTAATGCATTATTTCCAGCAAATCAGACAGTAATTCTTTTTACCGCGACGCAGTAAGGTAAAACGACCAAACAGACGATCTTCTTCTTTAAAGAAGTATTCAGGATCGGACTGTTTTTCACCGTTAAT  >  NC_000913/1715780‑1716067

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: