Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 1,772,240 | A→G | 6.7% | K390K (AAA→AAG) | ydiM → | putative MFS transporter, membrane protein |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,772,240 | 0 | A | G | 6.7% | 187.2 / 3.4 | 60 | K390K (AAA→AAG) | ydiM | putative MFS transporter, membrane protein |
Reads supporting (aligned to +/- strand): ref base A (25/31); new base G (2/2); total (27/33) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01 |
GCGGGCAGTATTGCGACCTTTACTATTCCGTTGATTACGGCTCATCTGTCCCAAAGAAGTATTGCCGATATTATGTGGTTCGATACCGCCATCGCTGCCATCGGTTTTTTACTGGCACTGTTTATCGGCTTACGCAGCCGCAAAAAAACGCGGCATCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTATGACATGCA > NC_000913/1772094‑1772383 | gCGGGCAGTATTGCGACCTTTACTATTCCGTTGATTACGGCTCATCTGTCCCAAAGAAGTATTGCCGATATTATGTGGTTCGATACCGCCATCGCTGCCATCGGTTTTTTACTGGCACTGTTTATCGGCTTACGCAGCCGCAAAAAAAc < 1:463551/149‑1 (MQ=255) aGTATTGCGACCTTTACTATTCCGTTGATTACGGCTCATCTGTCCCAAAGAAGTATTGCCGATATTATGTGGTTCGATACCGCCATCGCTGCCATCGGTTTTTTACTGGCACTGTTTATCGGCTTACGCAGCCGCAAAAAAACGCGGCa > 2:334008/1‑149 (MQ=255) tttACTATTCCGTTGATTACGGCTCATCTGTCCCAAAGAAGTATTGCCGATATTATGTGGTTCGATACCGCCATCGCTGCCATCGGTTTTTTACTGGCACTGTTTATCGGCTTACGCAGCCGCAAAAAAACGCGGCATCACTCGCTaaa > 2:89887/1‑149 (MQ=255) aCTATTCCGTTGATTACGGCTCATCTGTCCCAAAGAAGTATTGCCGATATTATGTGGTTCGATACCGCCATCGCTGCCATCGGTTTTTTACTGGCACTGTTTATCGGCTTACGCAGCCGCAAAAAAACGCGGCATCACTCGCTAAAGGa < 1:172042/149‑1 (MQ=255) cTATTCCGTTGATTACGGCTCATCTGTCCCAAAGAAGTATTGCCGATATTATGTGGTTCGATACCGCCATCGCTGCCATCGGTTTTTTACTGGCACTGTTTATCGGCTTACGCAGCCGCAAAAAAACGCGGCATCACTCGCTAAAGGaa < 2:531/149‑1 (MQ=255) tATTCCGTTGATTACGGCTCATCTGTCCCAAAGAAGTATTGCCGATATTATGTGGTTCGATACCGCCATCGCTGCCATCGGTTTTTTACTGGCACTGTTTATCGGCTTACGCAGCCGCAAAAAAACGCGGCATCACTCGCTAAAGGaaa > 3:86064/1‑149 (MQ=255) tATTCCGTTGATTACGGCTCATCTGTCCCAAAGAAGTATTGCCGATATTATGTGGTTCGATACCGCCATCGCTGCCATCGGTTTTTTACTGGCACTGTTTATCGGCTTACGCAGCCGCAAAAAAACGCGGCATCACTCGCTAAAGGaaa > 2:307566/1‑149 (MQ=255) aTTCCGTTGATTACGGCTCATCTGTCCCAAAGAAGTATTGCCGATATTATGTGGTTCGATACCGCCATCGCTGCCATCGGTTTTTTACTGGCACTGTTTATCGGCTTACGCAGCCGCAAAAAAACGCGGCATCACTCGCTAAAGGaaaa < 2:357554/149‑1 (MQ=255) ttACGGCTCATCTGTCCCAAAGAAGTATTGCCGATATTATGTGGTTCGATACCGCCATCGCTGCCATCGGTTTTTTACTGGCACTGTTTATCGGCTTACGCAGCCGCAAAAAAACGCGGCATCACTCGCTAAAGGAAAATGTCGCTCCg > 4:118053/1‑149 (MQ=255) tACGGCTCATCTGTCCCAAAGAAGTATTGCCGATATTATGTGGTTCGATACCGCCATCGCTGCCATCGGTTTTTTACTGGCACTGTTTATCGGCTTACGCAGCCGCAAAAAAACGCGGCATCACTCGCTAAAGGAAAATGTCGCTCCgg < 3:20021/149‑1 (MQ=255) ggCTCATCTGTCCCAAAGAAGTATTGCCGATATTATGTGGTTCGATACCGCCATCGCTGCCATCGGTTTTTTACTGGCACTGTTTATCGGCTTACGCAGCCGCAAAAAAACGCGGCATCACTCGCTAAAGGAAAATGTCGCTCCgggtg < 2:340504/149‑1 (MQ=255) cTCATCTGTCCCAAAGAAGTATTGCCGATATTATGTGGTTCGATACCGCCATCGCTGCCATCGGTTTTTTACTGGCACTGTTTATCGGCTTACGCAGCCGCAAAAAAACGCGGCATCACTCGCTAAAGGAAAATGTCGCTCCgggtggg < 2:363785/149‑1 (MQ=255) tCTGTCCCAAAGAAGTATTGCCGATATTATGTGGTTCGATACCGCCATCGCTGCCATCGGTTTTTTACTGGCACTGTTTATCGGCTTACGCAGCCGCAAAAAAACGCGGCATCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAAt > 4:194911/1‑149 (MQ=255) tGTCCCAAAGAAGTATTGCCGATATTATGTGGTTCGATACCGCCATCGCTGCCATCGGTTTTTTACTGGCACTGTTTATCGGCTTACGCAGCCGCAAAAAAACGCGGCATGACTCGCTAAAGGAAAATGTCGCTCCgggt < 4:148954/140‑1 (MQ=255) tGTCCCAAAGAAGTATTGCCGATATTATGTGGTTCGATACCGCCATCGCTGCCATCGGTTTTTTACTGGCACTGTTTATCGGCTTACGCAGCCGCAAAAAAACGCGGCATGACTCGCTAAAGGAAAATGTCGCTCCgggt > 3:148954/1‑140 (MQ=255) ccAAAGAAGTATTGCCGATATTATGTGGTTCGATACCGCCATCGCTGCCATCGGTTTTTTACTGGCACTGTTTATCGGCTTACGCAGCCGCAAAAAAACGCGGCATCACTCGCTAAAGGAAAATGTCGCTCTGGGTGGGTAATGCAata > 4:493754/1‑149 (MQ=255) ccAAAGAAGTATTGCCGATATTATGTGGTTCGATACCGCCATCGCTGCCATCGGTTTTTTACTGGCACTGTTTATCGGCTTACGCAGCCGCAAAAAAACGCGGCATCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAata > 1:549401/1‑149 (MQ=255) cAAAGAAGTATTGCCGATATTATGTGGTTCGATACCGCCATCGCTGCCATCGGTTTTTTACTGGCACTGTTTATCGGCTTACGCAGCCGCAAAAAAACGCGGCATCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAatat > 3:93053/1‑149 (MQ=255) gTATTGCCGATATTATGTGGTTCGATACCGCCATCGCTGCCATCGGTTTTTTACTGGCACTGTTTATCGGCTTACGCAGCCGCAAAAAAACGCGGCATCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTc < 4:364591/149‑1 (MQ=255) aTTGCCGATATTATGTGGTTCGATACCGCCATCGCTGCCATCGGTTTTTTACTGGCACTGTTTATCGGCTTACGCAGCCGCAAAAAAACGCGGCATCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAg < 3:415259/149‑1 (MQ=255) aTTGCCGATATTATGTGGTTCGATACCGCCATCGCTGCCATCGGTTTTTTACTGGCACTGTTTATCGGCTTACGCAGCCGCAAAAAAACGCGGCATCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAg < 2:549401/149‑1 (MQ=255) gCCGATATTATGTGGTTCGATACCGCCATCGCTGCCATCGGTTTTTTACTGGCACTGTTTATCGGCTTACGCAGCCGCAAAAAAACGCGGCATCACTCGCTa > 2:12900/1‑102 (MQ=255) gCCGATATTATGTGGTTCGATACCGCCATCGCTGCCATCGGTTTTTTACTGGCACTGTTTATCGGCTTACGCAGCCGCAAAAAAACGCGGCATCACTCGCTa < 1:12900/102‑1 (MQ=255) ccGATATTATGTGGTTCGATACCGCCATCGCTGCCATCGGTTTTTTACTGGCACTGTTTATCGGCTTACGCAGCCGCAAAAAAACGCGGCATCACTCGCTAAAGGaaaa < 4:165649/109‑1 (MQ=255) ccGATATTATGTGGTTCGATACCGCCATCGCTGCCATCGGTTTTTTACTGGCACTGTTTATCGGCTTACGCAGCCGCAAAAAAACGCGGCATCACTCGCTAAAGGaaaa > 3:165649/1‑109 (MQ=255) cGATATTATGTGGTTCGATACCGCCATCGCTGCCATCGGTTTTTTACTGGCACTGTTTATCGGCTTACGCAGCCGCAAAAAGACGCGGCAt < 1:392638/91‑1 (MQ=255) cGATATTATGTGGTTCGATACCGCCATCGCTGCCATCGGTTTTTTACTGGCACTGTTTATCGGCTTACGCAGCCGCAAAAAGACGCGGCAt > 2:392638/1‑91 (MQ=255) tagtatGTGGTTCGATACTGCCATCGCTGCCATCGGTTTTTTACTGGCACTGTTTATCGGCTTACGCAGCCGCAAAAAAACGCGGCATCACTCGCTGAAGCAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCa > 4:392155/4‑149 (MQ=255) atGTGGTTCGATACCGCCATCGCTGCCATCGGTTTTTTACTGGCACTGTTTATCGGCTTACGCAGCCGCAAAAAAACGCGGCATCACTCGCTGAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGAt < 3:392155/149‑1 (MQ=255) atGTGGTTCGATACCGCCATCGCTGCCATCGGTTTTTTACTGGCACTGTTTATCGGCTTACGCAGCCGCAAAAAAACGCGGCATCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGAt < 1:334008/149‑1 (MQ=255) atGTGGTTCGATACCGCCATCGCTGCCATCGGTTTTTTACTGGCACTGTTTATCGGCTTACGCAGCCGCAAAAAAACGCGGCATCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGAt < 1:593727/149‑1 (MQ=255) gtgGTTCGATACCGCCATCGCTGCCATCGGTTTTTTACTGGCACTGTTTATCGGCTTACGCAGCCGCAAAAAAACGCGGCATCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCt > 1:78685/1‑149 (MQ=255) ggTTCGATACCGCCATCGCTGCCATCGGTTTTTTACTGGCACTGTTTATCGGCTTACGCAGCCGCAAAAAAACGCGGCATCACTCGCTAAAg < 4:27200/92‑1 (MQ=255) ggTTCGATACCGCCATCGCTGCCATCGGTTTTTTACTGGCACTGTTTATCGGCTTACGCAGCCGCAAAAAAACGCGGCATCACTCGCTAAAg > 3:27200/1‑92 (MQ=255) gTTCGATACCGCCATCGCTGCCATCGGTTTTTTACTGGCACTGTTTATCGGCTTACGCAGCCGCAAAAAAACGCGGCATCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTAc < 1:75937/149‑1 (MQ=255) ttCGATACCGCCATCGCTGCCATCGGTTTTTTACTGGCACTGTTTATCGGCTTACGCAGCCGCAAAAAAACGCGGCATCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACg < 2:40492/149‑1 (MQ=255) cGATACCGCCATCGCTGCCATCGGTTTTTTACTGGCACTGTTTATCGGCTTACGCAGCCGCAAAAAAACGCGGCATCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGa > 1:446670/1‑149 (MQ=255) gCCATCGCTGCCATCGGTTTTTTACTGGCACTGTTTATCGGCTTACGCAGCCGCAAAAAAACGCGGCATCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGataaataa < 3:27942/149‑1 (MQ=255) tGCCATCGGTTTTTTACTGGCACTGTTTATCGGCTTACGCAGCCGCAAAAAAACGCGGCATCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCt < 1:89887/149‑1 (MQ=255) ccATCGGTTTTTTACTGGCACTGTTTATCGGCTTACGCAGCCGCAAAAAAACGCGGCATCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTgc > 3:420177/1‑149 (MQ=255) aTCGGTTTTTTACTGGCACTGTTTATCGGCTTACGCAGCCGCAAAAAAACGCGGCATCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTgcgc > 4:519548/1‑149 (MQ=255) tCGGTTTTTTACTGGCACTGTTTATCGGCTTACGCAGCCGCAAAAAAACGCGGCATCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGct > 2:82734/1‑149 (MQ=255) gTTTTTTACTGGCACTGTTTATCGGCTTACGCAGCCGCAAAAAAACGCGGCATCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGctaac > 1:336808/1‑149 (MQ=255) ttttACTGGCACTGTTTATCGGCTTACGCAGCCGCAAAAAAACGCGGCATCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGctaactaa < 4:93053/149‑1 (MQ=255) ttttACTGGCACTGTTTATCGGCTTACGCAGCCGCAAAAAAACGCGGCATCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGctaactaa < 3:102867/149‑1 (MQ=255) tttACTGGCACTGTTTATCGGCTTACGCAGCCGCAAAAAAACGCGGCATCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAg < 4:420177/149‑1 (MQ=255) ttACTGGCACTGTTTATCGGCTTACGCAGCCGCAAAAAAACGCGGCATCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAgg > 2:264187/1‑149 (MQ=255) gCACTGTTTATCGGCTTACGCAGCCGCAAAAAAACGCGGCATCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATc < 4:484385/149‑1 (MQ=255) tGTTTATCGGCTTACGCAGCCGCAAAAAAACGCGGCATCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGa > 1:350785/1‑149 (MQ=255) tttATCGGCTTACGCAGCCGCAAAAAAACGCGGCATCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATc > 1:179131/1‑149 (MQ=255) tCGGCTTACGCAGCCGCAAAAAGACGCGGCATCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGa < 2:183238/105‑1 (MQ=255) tCGGCTTACGCAGCCGCAAAAAGACGCGGCATCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGa > 1:183238/1‑105 (MQ=255) tCGGCTTACGCAGCCGCAAAAAAACGCGGCATCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCaaaa < 1:264187/149‑1 (MQ=255) gCTTACGCAGCCGCAAAAAAACGCGGCATCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCaaaaa > 2:329169/1‑147 (MQ=255) gCTTACGCAGCCGCAAAAAAACGCGGCATCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCaaaaa < 1:329169/147‑1 (MQ=255) cTTACGCAGCCGCAAAAAAACGCGGCATCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAaaacaa < 2:479745/149‑1 (MQ=255) ttACGCAGCCGCAAAAAAACGCGGCATCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTCTTCACGTCATGCAAGATCTTACGGATAAATACCTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAaaccaaa < 2:480576/149‑1 (MQ=255) cAAAAAAACGCGGCATCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAat > 4:118268/1‑58 (MQ=255) cAAAAAAACGCGGCATCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAat < 3:118268/58‑1 (MQ=255) cAAAAAAACGCGGCATCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTATGACATGc < 4:516375/149‑1 (MQ=255) aaaaaaaCGCGGCATCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTATGACATGCa > 1:304374/1‑149 (MQ=255) | GCGGGCAGTATTGCGACCTTTACTATTCCGTTGATTACGGCTCATCTGTCCCAAAGAAGTATTGCCGATATTATGTGGTTCGATACCGCCATCGCTGCCATCGGTTTTTTACTGGCACTGTTTATCGGCTTACGCAGCCGCAAAAAAACGCGGCATCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTATGACATGCA > NC_000913/1772094‑1772383 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |