Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,901,034 C→G 10.2% T184T (ACG→ACC)  yoaE ← putative membrane protein/conserved protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,901,0340CG10.2% 158.8 / 10.3 49T184T (ACG→ACC) yoaEputative membrane protein/conserved protein
Reads supporting (aligned to +/- strand):  ref base C (26/18);  new base G (2/3);  total (28/21)
Fisher's exact test for biased strand distribution p-value = 6.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.51e-02

ACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAACGAGAAGACGGCGT  >  NC_000913/1900890‑1901179
                                                                                                                                                |                                                                                                                                                  
aCGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTgg                                                                                                                                                >  4:82733/1‑149 (MQ=255)
 cgcgCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTgg‑g                                                                                                                                              <  1:421551/149‑1 (MQ=255)
      aaTCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACC‑CCACGGTGGTGGTGCt                                                                                                                                         <  2:275671/149‑1 (MQ=38)
       aTCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTg                                                                                                                                        <  4:260058/149‑1 (MQ=255)
       aTCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACC‑CCACGGTGGTGGTGCtc                                                                                                                                        <  4:531722/149‑2 (MQ=255)
             ttAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAAc                                                                                                                                  <  2:241982/149‑1 (MQ=255)
                     ttCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATcgcg                                                                                                                          >  4:29473/1‑149 (MQ=255)
                     ttCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATAATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATcgcg                                                                                                                          >  1:323359/1‑149 (MQ=255)
                          tgatgatCGAGAAGCCAATCGCGGCATACAGGTAACCTATCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGc                                                                                                                     <  4:389813/149‑1 (MQ=255)
                           gatgatCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACC‑CCACGGTGGTGGTGCtc                                                                                                                                        >  1:402653/1‑128 (MQ=255)
                           gatgatCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACC‑CCACGGTGGTGGTGCtc                                                                                                                                        <  2:402653/129‑2 (MQ=37)
                               atCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGTATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGttt                                                                                                                >  4:236496/1‑149 (MQ=255)
                               atCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGttt                                                                                                                <  4:412843/149‑1 (MQ=255)
                                tCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTg                                                                                                               >  2:495680/1‑149 (MQ=255)
                                  gagaAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGa                                                                                                             <  3:328835/149‑1 (MQ=255)
                                       gCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCa                                                                                                        >  2:93123/1‑149 (MQ=255)
                                          aaTCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCa                                                                                                     >  1:250883/1‑149 (MQ=255)
                                           aTCGCGGCATACAGGTAACCTTTCGGTATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGa                                                                                                             <  4:381737/140‑1 (MQ=255)
                                           aTCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGa                                                                                                             >  3:381737/1‑140 (MQ=255)
                                                        agTAACCTTTCGGAATGTGGGAACCGAAACCTTCTGCCACCAGAATCAGACCACTCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCCGCAACATAACCGCGATc                                                                                       >  4:118632/2‑149 (MQ=39)
                                                            aCCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCaa                                                                                   <  1:57243/149‑1 (MQ=255)
                                                               tttCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCa                                                                                >  4:503132/1‑149 (MQ=255)
                                                                    gAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTAcc                                                                           >  1:316163/1‑149 (MQ=255)
                                                                           gaaaccgaaaccTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCca                                                                    >  2:196409/1‑149 (MQ=255)
                                                                                cgaaaccTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCTGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCTGCAACATAGCCGCCATCGCAATCACTACCGCCGCCatcatc                                                               >  4:350672/1‑149 (MQ=255)
                                                                                cgaaaccTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAAcc                                                                                             <  2:364275/119‑1 (MQ=255)
                                                                                cgaaaccTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAAcc                                                                                             >  1:364275/1‑119 (MQ=255)
                                                                                 gaaaccTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCAtcatca                                                              >  1:525036/1‑149 (MQ=255)
                                                                                  aaaccTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCAc                                                             >  4:539283/1‑149 (MQ=255)
                                                                                       ttCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCa                                                        <  1:93123/149‑1 (MQ=255)
                                                                                       ttCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCa                                                        >  2:237833/1‑149 (MQ=255)
                                                                                              accaGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGtt                                                 >  2:247380/1‑149 (MQ=255)
                                                                                               ccaGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTa                                                <  2:333628/149‑1 (MQ=255)
                                                                                                  gACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAAcc                                             <  4:246929/149‑1 (MQ=255)
                                                                                                     tCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATc                                          >  4:71483/1‑149 (MQ=255)
                                                                                                      cAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATcc                                         <  1:495680/149‑1 (MQ=255)
                                                                                                           cAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGcc                                                                                                         >  1:79489/1‑80 (MQ=255)
                                                                                                           cAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGcc                                                                                                         <  2:79489/80‑1 (MQ=255)
                                                                                                                    aCAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAAcc                                                                                             >  4:397078/1‑83 (MQ=255)
                                                                                                                    aCAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAAcc                                                                                             <  3:397078/83‑1 (MQ=255)
                                                                                                                    aCAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAAtt                           <  2:127810/149‑1 (MQ=255)
                                                                                                                       ggAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTAcc                        >  1:330698/1‑149 (MQ=255)
                                                                                                                             tCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTAc                         <  2:175548/142‑1 (MQ=255)
                                                                                                                             tCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTAc                         >  1:175548/1‑142 (MQ=255)
                                                                                                                              cagacagaGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCa                 >  3:247541/1‑149 (MQ=255)
                                                                                                                               ttaaccagCACC‑CCACGGTGGTGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCaa                >  2:570520/7‑149 (MQ=255)
                                                                                                                                   agagCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAACgag            >  3:149011/1‑149 (MQ=255)
                                                                                                                                   agagCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAACgag            >  2:268304/1‑149 (MQ=255)
                                                                                                                                   agagCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCAACCAACgag            <  3:539283/149‑1 (MQ=255)
                                                                                                                                        accaccaccGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAACGAGAAGAc       >  3:218548/1‑149 (MQ=255)
                                                                                                                                            ccaccGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAACGAGAAGACGGCg   >  1:323296/1‑149 (MQ=255)
                                                                                                                                             caccGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAACGAGAAGACGGCGt  >  3:47394/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
ACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGG‑GGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAACGAGAAGACGGCGT  >  NC_000913/1900890‑1901179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑