Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,906,231 C→A 6.3% L182I (CTC→ATC)  mntP → putative Mn(2+) efflux pump, mntR‑regulated

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,906,2310CA 6.3% 201.9 / 2.3 59L182I (CTC→ATC) mntPputative Mn(2+) efflux pump, mntR‑regulated
Reads supporting (aligned to +/- strand):  ref base C (27/28);  new base A (2/2);  total (29/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTATCGCGACCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAAT  >  NC_000913/1906084‑1906374
                                                                                                                                                   |                                                                                                                                               
aTTATCGCGACCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCct                                                                                                                                                <  1:5019/149‑1 (MQ=255)
 ttATCGCGACCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCctc                                                                                                                                               <  2:161605/149‑1 (MQ=255)
 ttATCGCGACCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCctc                                                                                                                                               >  1:396316/1‑149 (MQ=255)
 ttATCGCGACCGCATTGGCCATTGGTTGTGCAACCTTGATTATGACAACATTAGGGATGATGGTTGGTAGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCctc                                                                                                                                               <  3:501003/149‑1 (MQ=255)
     cgcgACCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGa                                                                                                                                           <  4:446931/149‑1 (MQ=255)
        gACCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGAGGATCTGGACGc                                                                                                                                        <  4:101067/149‑1 (MQ=255)
               ttGGCCATTTGTTGTGCAACATTGCTTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGACTCAATTATTTGGAAAAAAGCGGAAATTATAGGCGGGCTTGTGCTGATCGGCATCGGCGTCCAGATCATCTGGACGAACTTCCa                                                                                                                                 >  4:301783/1‑149 (MQ=255)
               ttGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCa                                                                                                                                 <  1:116226/149‑1 (MQ=255)
                  gCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACgg                                                                                                                              >  1:542383/1‑149 (MQ=255)
                   ccATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGt                                                                                                                             <  2:396316/149‑1 (MQ=255)
                   ccATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGt                                                                                                                             <  4:174498/149‑1 (MQ=255)
                      ttGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTtaa                                                                                                                          >  3:375647/1‑149 (MQ=255)
                        ggTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTtaata                                                                                                                        >  1:225770/1‑149 (MQ=255)
                         gTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTtaataa                                                                                                                       >  2:103060/1‑149 (MQ=255)
                         gTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTtaataa                                                                                                                       <  4:202374/149‑1 (MQ=255)
                               cAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGc                                                                                                                 >  2:298683/1‑149 (MQ=255)
                                aaCCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCt                                                                                                                >  1:280706/1‑149 (MQ=255)
                                aaCCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCt                                                                                                                >  2:43644/1‑149 (MQ=255)
                                 aCCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTg                                                                                                               >  1:589121/1‑149 (MQ=255)
                                      gATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCaga                                                                                                          <  3:293973/149‑1 (MQ=255)
                                       aTTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCagag                                                                                                         <  1:43644/149‑1 (MQ=255)
                                        ttATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAgg                                                                                                        >  2:518573/1‑149 (MQ=255)
                                            gTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGaa                                                                                                    <  4:546623/149‑1 (MQ=255)
                                            gTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGaa                                                                                                    <  2:526109/149‑1 (MQ=255)
                                             tCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAat                                                                                                   >  4:527528/1‑149 (MQ=255)
                                              cAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAata                                                                                                  <  1:23498/149‑1 (MQ=255)
                                              cAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAata                                                                                                  <  1:298683/149‑1 (MQ=255)
                                                  aTTAGGGATGATGGTTGGTCGCTTTATCGGCTGAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATAGGCATCCAGATACTCTGGACGCACTTCTACGGTTAATAAGAACGCGGCCAGAGGTGAATATTaa                                                                                              >  2:286186/1‑149 (MQ=255)
                                                   ttAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTaaa                                                                                             <  1:247868/149‑1 (MQ=255)
                                                      gggAGGAGGGGTGGTCGCTTTATCCGCTCAATTAGTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTc                                                                                          >  4:111573/1‑149 (MQ=255)
                                                         atgatgGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGt                                                                                       <  4:248473/149‑1 (MQ=255)
                                                          tgatgGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTc                                                                                      <  2:537834/149‑1 (MQ=255)
                                                            atgGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTg                                                                                    >  1:5199/1‑149 (MQ=255)
                                                               gttggtCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCa                                                                                 <  1:518573/149‑1 (MQ=255)
                                                                  ggtCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTc                                                                              <  1:321821/149‑1 (MQ=255)
                                                                   gtCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTaaa                                                                                             >  4:263879/1‑133 (MQ=255)
                                                                   gtCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTaaa                                                                                             <  3:263879/133‑1 (MQ=255)
                                                                   gtCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCg                                                                             >  2:281450/1‑149 (MQ=255)
                                                                            tCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACActtct                                                                    >  2:164391/1‑149 (MQ=255)
                                                                              ggCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCttt                                                                  <  3:504874/149‑1 (MQ=255)
                                                                              ggCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCttt                                                                  >  2:132748/1‑149 (MQ=255)
                                                                               gCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCtttt                                                                 >  3:253505/1‑149 (MQ=255)
                                                                                 tCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTtgc                                                               >  1:558444/1‑149 (MQ=255)
                                                                                   aaTTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTtgctg                                                             >  2:88099/1‑149 (MQ=255)
                                                                                    attattGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTtgctgc                                                            <  2:225770/149‑1 (MQ=255)
                                                                                            gAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGttt                                                    <  3:242322/149‑1 (MQ=255)
                                                                                                aaaGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCa                                                <  2:539088/149‑1 (MQ=255)
                                                                                                        aaTTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTg                                        <  1:132748/149‑1 (MQ=255)
                                                                                                          ttCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGc                                      <  2:596805/149‑1 (MQ=255)
                                                                                                               ggcggGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGc                                 <  3:111573/149‑1 (MQ=255)
                                                                                                               ggcggGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGc                                 >  2:94571/1‑149 (MQ=255)
                                                                                                                      tGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAg                          <  2:188393/149‑1 (MQ=255)
                                                                                                                       ggTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAgg                         <  1:94571/149‑1 (MQ=255)
                                                                                                                           cTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCaaa                     >  4:331852/1‑149 (MQ=255)
                                                                                                                                      cggcGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCt          >  4:330942/1‑149 (MQ=255)
                                                                                                                                      cggcGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCt          <  4:375647/149‑1 (MQ=255)
                                                                                                                                        gcGTCCAGAGGATCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCTGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGa                                              >  4:40874/1‑111 (MQ=255)
                                                                                                                                        gcGTCCAGAGGATCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGGCAGTGTTTGCCAGa                                              <  3:40874/111‑1 (MQ=255)
                                                                                                                                             cAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCaataa   >  4:160820/1‑149 (MQ=255)
                                                                                                                                              aGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCaataat  >  1:236595/1‑149 (MQ=255)
                                                                                                                                              aGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCaataat  >  4:568138/1‑149 (MQ=255)
                                                                                                                                               gATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGt                                                                                                       <  3:242345/47‑1 (MQ=255)
                                                                                                                                               gATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGt                                                                                                       >  4:242345/1‑47 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
ATTATCGCGACCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAAT  >  NC_000913/1906084‑1906374

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑