Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 217,037 A→T 6.2% intergenic (‑34/+20) yaeF ← / ← proS putative lipoprotein/prolyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913217,0370AT 6.2% 257.2 / 4.1 76intergenic (‑34/+20)yaeF/proSputative lipoprotein/prolyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base A (39/32);  new base T (2/3);  total (41/35)
Fisher's exact test for biased strand distribution p-value = 6.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTATATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATA  >  NC_000913/216893‑217183
                                                                                                                                                |                                                                                                                                                  
ggCATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCTTTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCa                                                                                                                                                <  4:174972/149‑1 (MQ=255)
ggCATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCa                                                                                                                                                <  1:344730/149‑1 (MQ=255)
 gCATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAg                                                                                                                                               <  1:456230/149‑1 (MQ=255)
  cATCTACTGCTGTGGCTGAGGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGTCCTGAATTTCAgg                                                                                                                                              <  3:489612/149‑1 (MQ=255)
            tgtgGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTg                                                                                                                                    <  2:253121/149‑1 (MQ=255)
                gCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCAt                                                                                                                                >  2:593722/1‑149 (MQ=255)
                  tGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCa                                                                                                                              <  2:354218/149‑1 (MQ=255)
                      gggTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTg                                                                                                                                    <  2:555124/139‑1 (MQ=255)
                      gggTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTg                                                                                                                                    >  1:555124/1‑139 (MQ=255)
                        gTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCttt                                                                                                                        >  4:33474/1‑149 (MQ=255)
                        gTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCttt                                                                                                                        >  4:379460/1‑149 (MQ=255)
                        gTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCttt                                                                                                                        >  2:453198/1‑149 (MQ=255)
                         tCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTa                                                                                                                       >  2:573111/1‑149 (MQ=255)
                          ctggctggCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTaa                                                                                                                      <  2:334562/149‑1 (MQ=255)
                          ctggctggCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTaa                                                                                                                      <  3:154524/149‑1 (MQ=255)
                            ggctggctGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATc                                                                                                                    <  1:572654/149‑1 (MQ=255)
                            ggctggctGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATc                                                                                                                    <  2:552812/149‑1 (MQ=255)
                            ggctggctGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATc                                                                                                                    <  4:284361/149‑1 (MQ=255)
                               tggctgATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGt                                                                                                                 <  1:546481/149‑1 (MQ=255)
                                   tgATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGTCCTGAATTTCAGGGcc                                                                                                                                           >  1:395232/1‑119 (MQ=255)
                                   tgATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGTCCTGAATTTCAGGGcc                                                                                                                                           <  2:395232/119‑1 (MQ=255)
                                      tatCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCa                                                                                                          <  4:9085/149‑1 (MQ=255)
                                        tCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGa                                                                                                        >  4:84601/1‑149 (MQ=255)
                                          aaCGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGata                                                                                                      >  4:101433/1‑149 (MQ=255)
                                              gTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTcg                                                                                                  >  4:312449/1‑149 (MQ=255)
                                               tACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTcga                                                                                                 <  3:101433/149‑1 (MQ=255)
                                                       gAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGTCCTGAATTTCGGGGcc                                                                                                                                           <  4:558921/99‑1 (MQ=255)
                                                       gAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGTCCTGAATTTCAGGGcc                                                                                                                                           >  3:558921/1‑99 (MQ=255)
                                                           gTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAg                                                                                     >  2:397172/1‑149 (MQ=255)
                                                           gTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAg                                                                                     <  1:593722/149‑1 (MQ=255)
                                                           gTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAg                                                                                     >  3:450306/1‑149 (MQ=255)
                                                              aaagaaagCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCt                                                                                  >  4:79010/1‑149 (MQ=255)
                                                                agaaagCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCtta                                                                                >  2:149904/1‑149 (MQ=255)
                                                                 gaaagCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCtcaa                                                                               >  1:46315/1‑146 (MQ=255)
                                                                  aaagCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCttaat                                                                              <  3:79010/149‑1 (MQ=255)
                                                                      cTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAAc                                                                          <  1:134009/149‑1 (MQ=255)
                                                                          ggAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGtt                                                                      >  3:174601/1‑149 (MQ=255)
                                                                           gagaAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGttt                                                                     >  2:591749/1‑149 (MQ=255)
                                                                                aCAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGc                                                                <  1:573111/149‑1 (MQ=255)
                                                                                          gTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCACTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGcgacg                                                      >  2:12667/1‑149 (MQ=255)
                                                                                           tACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGcgacga                                                     >  2:224892/1‑149 (MQ=255)
                                                                                            aCGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGat                                                    <  2:576446/149‑1 (MQ=255)
                                                                                            aCGCCTTGGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGat                                                    >  2:325192/1‑149 (MQ=255)
                                                                                              gCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGatat                                                  <  4:174601/149‑1 (MQ=255)
                                                                                              gCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGatat                                                  >  4:357578/1‑149 (MQ=255)
                                                                                               ccTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATAtt                                                 >  1:592933/1‑149 (MQ=255)
                                                                                                      tttATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTATATTc                                          >  1:467501/1‑149 (MQ=255)
                                                                                                        tATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTATATTCGa                                        <  4:241191/149‑1 (MQ=255)
                                                                                                            cATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTATATTCGATAtc                                    >  4:66572/1‑149 (MQ=255)
                                                                                                              ttttATACCATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCTTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTATATTCGATAtcgt                                  <  1:356438/149‑1 (MQ=255)
                                                                                                              ttttATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTATATTCGATAtcgt                                  >  1:122676/1‑149 (MQ=255)
                                                                                                                 tataCAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTATATTCGATATcgtcgt                               >  1:318203/1‑149 (MQ=255)
                                                                                                                   taCAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTATATTCGATATCGTCGTTg                             >  1:393499/1‑149 (MQ=255)
                                                                                                                    aCAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTATATTCGATATCGTCGTTGt                            >  2:589001/1‑149 (MQ=255)
                                                                                                                         ccATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTATATTCGATATCGTCGTTGTCGAgg                       <  1:591749/149‑1 (MQ=255)
                                                                                                                             gTAAAAAAAGGGCCCTGAGATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCttaat                                                                              <  1:592318/90‑1 (MQ=255)
                                                                                                                             gTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCttaat                                                                              >  2:592318/1‑90 (MQ=255)
                                                                                                                                aaaaaaGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTATATTCGATATCGTCGTTGTCGAGGTTACg                  >  4:98131/1‑147 (MQ=255)
                                                                                                                                aaaaaaGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTATATTCGATATCGTCGTTGTCGAGGTTACGGt                <  4:507308/149‑1 (MQ=255)
                                                                                                                                aaaaaaGGGCCCTGAAATGCATGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTATATTCGATATCGTCGTTGTCGAGGTTACg                  <  3:98131/147‑1 (MQ=255)
                                                                                                                                 aaaaaGGGCGCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTATATGCGATAtcgtc                                 >  4:504360/1‑131 (MQ=255)
                                                                                                                                 aaaaaGGGCCCTGAAATTCAGGACCCTCTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTATATTCGATAtcgtc                                 <  3:504360/131‑1 (MQ=255)
                                                                                                                                  aaaaGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTATATTCGATATCGTCGTTGTCGAGGTTACGGTCg              <  1:12667/149‑1 (MQ=255)
                                                                                                                                    aaGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTATATTCGATATCGTCGTTGTCGAGGTTACGGTCGcc            <  4:30395/149‑1 (MQ=255)
                                                                                                                                     aGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTATATTCGATATCGTCGTTGTCGAGGTTACGGTCGccc           >  4:444785/1‑149 (MQ=255)
                                                                                                                                     aGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTATATTCGATATCGTCGTTGTCGAGGTTACGGTCGccc           <  2:467501/149‑1 (MQ=255)
                                                                                                                                         cccTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTATATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGca       <  3:507591/149‑1 (MQ=255)
                                                                                                                                         cccTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTATATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGca       >  4:507591/1‑149 (MQ=255)
                                                                                                                                         cccTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTATATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGca       >  3:456582/1‑149 (MQ=255)
                                                                                                                                          ccTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTATATTCGATAtcgtc                                 <  4:272122/122‑1 (MQ=255)
                                                                                                                                          ccTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTATATTCGATAtcgtc                                 >  3:272122/1‑122 (MQ=255)
                                                                                                                                          ccTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTATATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGcac      >  3:513996/1‑149 (MQ=255)
                                                                                                                                          ccTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTATATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGcac      >  3:311768/1‑149 (MQ=255)
                                                                                                                                           cTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTATATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGcaca     >  4:201543/1‑149 (MQ=255)
                                                                                                                                            tGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTACCTGTTTCTCGCCGTTGCGACGATATTTATATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACaa    >  1:164725/1‑149 (MQ=255)
                                                                                                                                            tGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTATATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACaa    >  4:307469/1‑149 (MQ=255)
                                                                                                                                              aaaTTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTATATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATa  <  2:592933/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
GGCATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTATATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATA  >  NC_000913/216893‑217183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑