Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,288,232 T→C 5.8% pseudogene (683/2525 nt) yejO ← pseudogene, autotransporter outer membrane homology;putative transport; Not classified; putative ATP‑binding component of a transport system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,288,2320TC 5.8% 221.5 / 3.8 69pseudogene (683/2525 nt)yejOpseudogene, autotransporter outer membrane homology;putative transport; Not classified; putative ATP‑binding component of a transport system
Reads supporting (aligned to +/- strand):  ref base T (31/34);  new base C (2/2);  total (33/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.25e-01

TGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATACTAAATTGGCCTTTACTGTTTGGACCACTCACTTCCAGCGCATTCGTTGAGACAATTAATTTTCCGCCTGCTTTTTTATCAACGCCAGTTACAGTCCCGCCATCCATAACCTCCAGTAAGCCGCCACTATCTACAGTGGTATTATAAGCGAAGTCATTTTCTTCAACTCGCAAACTTCCGCCGTTTTCCAACAACA  >  NC_000913/2288086‑2288380
                                                                                                                                                  |                                                                                                                                                     
tGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATACTAAATTGGCCTTTACTGTTTGGACCACTCACTTCCAGCGCATTCGTTga                                                                                                                                                     <  3:183256/149‑1 (MQ=255)
tGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATACTAAATTGGCCTTTACTGTTTGGACCACTCACTTCCAGCGCATTCGTTga                                                                                                                                                     <  4:304158/149‑1 (MQ=255)
tGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATACTAAATTGGCCTTTACTGTTTGGACCACTCACTTCCAGCGCATTCGTTga                                                                                                                                                     <  2:414084/149‑1 (MQ=255)
tGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATACTAAATTGGCCTTTACTGTTTGGACCACTCACTTCCAGCGCATTCGTTga                                                                                                                                                     <  2:16449/149‑1 (MQ=255)
tGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATACTAAATTGGCCTTTACTGTTTGGACCACTCACTTCCAGCGCATTCGTTga                                                                                                                                                     <  2:155542/149‑1 (MQ=255)
   ttATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATACTAAATTGGCCTTTACTGTTTGGACCACTCACTTCCAGCGCATTCGTTGAGAc                                                                                                                                                  >  1:407506/1‑149 (MQ=255)
              aGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATACTAAATTGGCCTTTACTGTTTGGACCACTCACTTCCAGCGCATTCGTTGAGACAATTAATTTTc                                                                                                                                       <  4:223449/149‑1 (MQ=255)
               gTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATACTAAATTGGCCTTTACTGTTTGGACCACTCACTTCCAGCGCATTCGTTGAGACAATTAATTTTcc                                                                                                                                      <  2:163742/149‑1 (MQ=255)
                tATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATACTAAATTGGCCTTTACTGTTTGGACCACTCACTTCCAGCGCATTCGTTGAGACAATTAATTTTCCg                                                                                                                                     >  1:340949/1‑149 (MQ=255)
                tATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATACTAAATTGGCCTTTACTGTTTGGACCACTCACTTCCAGCGCATTCGTTGAGACAATTAATTTTCCg                                                                                                                                     >  3:426728/1‑149 (MQ=255)
                   cAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATACTAAATTGGCCTTTACTGTTTGGACCACTCACTTCCAGCGCATTCGTTGAGACAATTAATTTTCCGCCt                                                                                                                                  >  4:330710/1‑149 (MQ=255)
                      tGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATACTAAATTGGCCTTTACTGTTTGGACCACTCACTTCCAGCGCATTCGTTGAGACAATTAATTTTCCGCCTGCt                                                                                                                               >  3:414008/1‑149 (MQ=255)
                      tGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATACTAAATTGGCCTTTACTGTTTGGACCACTCACTTCCAGCGCATTCGTTGAGACAATTAATTTTCCGCCTGCt                                                                                                                               <  3:509017/149‑1 (MQ=255)
                          cTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATACTAAATTGGCCTTTACTGTTTGGACCACTCACTTCCAGCGCATTCGTTGAGACAATTAATTTTCCGCCTGCttttt                                                                                                                           <  3:129391/149‑1 (MQ=255)
                          cTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATACTAAATTGGCCTTTACTGTTTGGACCACTCACTTCCAGCGCATTCGTTGAGACAATTAATTTTCCGCCTGCttttt                                                                                                                           >  1:484429/1‑149 (MQ=255)
                           tGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATACTAAATTGGCCTTTACTGTTTGGACCACTCACTTCCAGCGCATTCGTTGAGACAATTAATTTTCCGCCTGCtttttt                                                                                                                          <  2:134047/149‑1 (MQ=255)
                              gtgtCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATACTAAATTGGCCTTTACTGTTTGGACCACTCACTTCCAGCGCATTCGTTGAGACAATTAATTTTCCGCCTGCTTTTTTATc                                                                                                                       <  3:525431/149‑1 (MQ=255)
                                    tccATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATACTAAATTGGCCTTTACTGTTTGGACCACTCACTTCCAGCGCATTCGTTGAGACAATTAATTTTCCGCCTGCTTTTTTATCAACGcc                                                                                                                 >  1:117435/1‑149 (MQ=255)
                                    tccATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATACTAAATTGGCCTTTACTGTTTGGACCACTCACTTCCAGCGCATTCGTTGAGACAATTAATTTTCCGCCTGCTTTTTTATCAACGcc                                                                                                                 >  4:474389/1‑149 (MQ=255)
                                    tccATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATACTAAATTGGCCTTTACTGTTTGGACCACTCACTTCCAGCGCATTCGTTGAGACAATTAATTTTCCGCCTGCTTTTTTATCAACGcc                                                                                                                 >  4:141360/1‑149 (MQ=255)
                                      cATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATACTAAATTGGCCTTTACTGTTTGGACCACTCACTTCCAGCGCATTCGTTGAGACAATTAATTTTCCGCCTGCTTTTTTATCAACGCCAg                                                                                                               >  3:450512/1‑149 (MQ=255)
                                        tAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATACTAAATTGGCCTTTACTGTTTGGACCACTCACTTCCAGCGCATTCGTTGAGACAATTAATTTTCCGCCTGCTTTTTTATCAACGCCAGtt                                                                                                             >  1:91761/1‑149 (MQ=255)
                                        tAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATACTAAATTGGCCTTTACTGTTTGGACCACTCACTTCCAGCGCATTCGTTGAGACAATTAATTTTCCGCCTGCTTTTTTATCAACGCCAGtt                                                                                                             >  3:440215/1‑149 (MQ=255)
                                         aaCAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATACTAAATTGGCCTTTACTGTTTGGACCACTCACTTCCAGCGCATTCGTTGAGACAATTAATTTTCCGCCTGCTTTTTTATCAACGCCAGTTa                                                                                                            >  2:200127/1‑149 (MQ=255)
                                           cAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATACTAAATTGGCCTTTACTGTTTGGACCACTCACTTCCAGCGCATTCGTTGAGACAATTAATTTTCCGCCTGCTTTTTTATCAACGCCAGTTACa                                                                                                          <  2:117435/149‑1 (MQ=255)
                                           cAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATACTAAATTGGCCTTTACTGTTTGGACCACTCACTTCCAGCGCATTCGTTGAGACAATTAATTTTCCGCCTGCTTTTTTATCAACGCCAGTTACa                                                                                                          <  2:53773/149‑1 (MQ=255)
                                                aGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATACTAAATTGGCCTTTACTGTTTGGACCACTCACTTCCAGCGCATTCGTTGAGACAATTAATTTTCCGCCTGCTTTTTTATCAACGCCAGTTACAGTccc                                                                                                     >  4:167195/1‑149 (MQ=255)
                                                aGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATACTAAATTGGCCTTTACTGTTGGACCACTCACTTCCAGCGCATTCGTTGAGACAATTAATTTTCCGCCTGCTTTTTTATCAACGCCAGTTACAGTCCCg                                                                                                    >  2:249628/1‑149 (MQ=255)
                                                       aaCCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATACTAAATTGGCCTTTACTGTTTGGACCACTCACTTCCAGCGCATTCGTTGAGACAATTAATTTTCCGCCTGCTTTTTTATCAACGCCAGTTACAGTCCCGccatcc                                                                                              <  4:173217/149‑1 (MQ=255)
                                                            tcatcCAGTTCATAATTTTTTGACACACCATCTTTTATACTAAATTGGCCTTTACTGTTTGTACCACTCACTTCCTGCGCATGCGTTGAGACAATTAATTTTCCGCCTGCTAATTTATCAACGCCAGTTACAGTCCCGCCATCCATAAc                                                                                         <  4:127770/149‑1 (MQ=255)
                                                               tcCAGTTCATAATTTTTTGACACACCATCTTTTATACTAAATTGGCCTTTACTGTTTGGACCACTCACTTCCAGCGCATTCGTTGAGACAATTAATTTTCCGCCTGCtt                                                                                                                              <  1:148720/109‑1 (MQ=255)
                                                               tcCAGTTCATAATTTTTTGACACACCATCTTTTATACTAAATTGGCCTTTACTGTTTGGACCACTCACTTCCAGCGCATTCGTTGAGACAATTAATTTTCCGCCTGCtt                                                                                                                              >  2:148720/1‑109 (MQ=255)
                                                                 cAGTTCATAATTTTTTGACACACCATCTTTTATACTAAATTGGCCTTTACTGTTTGGACCACTCACTTCCAGCGCATTCGTTGAGACAATTAATTTTCCGCCTGCTTTTTTATCAACGCCAGTTACAGTc                                                                                                       <  4:513876/130‑1 (MQ=255)
                                                                 cAGTTCATAATTTTTTGACACACCATCTTTTATACTAAATTGGCCTTTACTGTTTGGACCACTCACTTCCAGCGCATTCGTTGAGACAATTAATTTTCCGCCTGCTTTTTTATCAACGCCAGTTACAGTCCCGCCATCCATAACCTCCa                                                                                    <  4:62444/149‑1 (MQ=255)
                                                                 cAGTTCATAATATTTTGACACACCATCTTTTATACTAAATTGGCCTTTACTGTTTGGACCACTCACTTCCAGCGCATTCGTTGAGACAATTAATTTTCCGCCTGCTTTTTTATCAACGCCAGTTACAGTc                                                                                                       >  3:513876/1‑130 (MQ=255)
                                                                     tCATAATTTTTTGACACACCATCTTTTATACTAAATTGGCCTTTACTGTTTGGACCACTCACTTCCAGCGCATTCGTTGAGACAATTAATTTTCCGCCTGCTTTTTTATCAACGCCAGTTACAGTCCCGCCATCCATAACCTCCAGTaa                                                                                <  1:200127/149‑1 (MQ=255)
                                                                     tCATAATTTTTTGACACACCATCTTTTATACTAAATTGGCCTTTACTGTTTGGACCACTCACTTCCAGCGCATTCGTTGAGACAATTAATTTTCCGCCTGCTTTTTTATCAACGCCAGTTACAGTCCCGCCATCCATAACCTCCAGTaa                                                                                <  3:330710/149‑1 (MQ=255)
                                                                      cATAATTTTTTGACACACCATCTTTTATACTAAATTGGCCTTTACTGTTTGGACCACTCACTTCCAGCGCATTCGTTGAGACAATTAATTTTCCGCCTGCTTTTTTATCAACGCCAGTTACAGTCCCGCCATCCATAACCTCCAGTAAg                                                                               <  1:571354/149‑1 (MQ=255)
                                                                       aTAATTTTTTGACACATCATCTTTTATACTAAATTGGCCTTTACTGTTTGGACCACTCACTTCCAGCGCATTCGTTGAGACAATTAATTTTCCGCCTGCTTTTTTATCAACGCCAGTTACAGTCCCGCCATCCATAACCTCCAGTAAgc                                                                              <  1:460623/149‑1 (MQ=255)
                                                                                 gACACACCATCTTTTATACTAAATTGGCCTTTACTGTTTGGACCACTCACTTCCAGCGCATTCGTTGGGACAATTAATTTTCCGCCTGCTTTTTTATTAACGCCAGTTACAGTCCCGCCATCCATAACCTCCAGTAAGCCGCCACTATc                                                                    <  2:510849/149‑1 (MQ=255)
                                                                                    acacCATCTTTTATACTAAATTGGCCTTTACTGTTTGGACCACTCACTTCCAGCGCATTCGTTGAGACAATTAATTTTCCGCCTGCTTTTTTATCAACGCCAGTTACAGTCCCGCCATCCATAACCTCCAGTAAGCCGCCACTATCTAc                                                                 >  4:364500/1‑149 (MQ=255)
                                                                                     cacCATCTTTTATACTAAATTGGCCTTTACTGTTTGGACCACTCACTTCCAGCGCATTCGTTGAGACAATTAATTTTCCGCCTGCTTTTTTATCAACGCCAGTTACAGTCCCGCCATCCATAACCTCCAGTAAGCCGCCACTATCTACa                                                                >  4:163138/1‑149 (MQ=255)
                                                                                      acCATCTTTTATACTAAATTGGCCTTTACTGTTTGGACCACTCACTTCCAGCGCATTCGTTGAGACAATTAATTTTCCGCCTGCTTTTTTATCAACGCCAGTTACAGTCCCGCCATCCATAACCTCCAGTAAGCCGCCACTATCTACAg                                                               >  3:25993/1‑149 (MQ=255)
                                                                                       ccATCTTTTATACTAAATTGGCCTTTACTGTTTGGACCACTCACTTCCAGCGCATTCGTTGAGACAATTAATTTTCCGCCTGCTTTTTTATCAACGCCAGTTACAGTCCCGCCATCCATAACCTCCAGTAAGCCGCCACTATCTACAGt                                                              >  1:27708/1‑149 (MQ=255)
                                                                                       ccATCTTTTATACTAAATTGGCCTTTACTGTTTGGACCACTCACTTCCAGCGCATTCGTCGAGACAATTAATTTTCCGCCTg                                                                                                                                 <  4:271049/82‑1 (MQ=255)
                                                                                       ccATCTTTTATACTAAATTGGCCTTTACTGTTTGGACCACTCACTTCCAGCGCATTCGTCGAGACAATTAATTTTCCGCCTg                                                                                                                                 >  3:271049/1‑82 (MQ=255)
                                                                                           cTTTTATACTAAATTGGCCTTTACTGTTTGGACCACTCACTTCCAGCGCATTCGTTGAGACAATTAATTTTCCGCCTGCTTTTTTATCAACGCCAGTTACAGTCCCGCCATCCATAACCTCCAGTAAGCCGCCACTATCTACAGTGGta                                                          <  2:568376/149‑1 (MQ=255)
                                                                                           cTTTTATACTAAATTGGCCTTTACTGTTTGGACCACTCACTTCCAGCGCATTCGTTGAGACAATTAATTTTCCGCCTGCTTTTTTATCAACGCCAGTTACAGTCCCGCCATCCATAACCTCCAGTAAGCCGCCACTATCTACAGTGGta                                                          <  1:405154/149‑1 (MQ=255)
                                                                                            ttttATACTAAATTGGCCTTTACTGTTTGGACCACTCACTTCCAGCGCATTCGTTGAGACAATTAATTTTCCGCCTGCTTTTTTATCAACGCCAGTTACAGTCCCGCCATCCATAACCTCCAGTAAGCCGCCACTATCTACAGTGGtat                                                         >  4:241277/1‑149 (MQ=255)
                                                                                             tttATACTAAATTGGCCTTTACTGTTTGGACCACTCACTTCCAGCGCATTCGTTGAGACAATTAATTTTCCGCCTGCTTTTTTATCAACGCCAGTTACAGTCCCGCCATCCATAACCTCCAGTAAGCCGCCACTATCTACAGTGGtatt                                                        <  3:141360/149‑1 (MQ=255)
                                                                                              ttATACTAAATTGGCCTTTACTGTTTGGACCACTCACTTCCAGCGCATTCGTTGAGACAATTAATTTTCCGCCTGCTTTTTTATCAACGCCAGTTACAGTCCCGCCATCCATAACCTCCAGTAAGCCGCCACTATCTACAGt                                                              <  3:196047/142‑1 (MQ=255)
                                                                                              ttATACTAAATTGGCCTTTACTGTTTGGACCACTCACTTCCAGCGCATTCGTTGAGACAATTAATTTTCCGCCTGCTTTTTTATCAACGCCAGTTACAGTCCCGCCATCCATAACCTCCAGTAAGCCGCCACTATCTACAGt                                                              >  4:196047/1‑142 (MQ=255)
                                                                                                  aCTAAATTGGCCTTTACTGTTTGGACCACTCACTTCCAGCGCATTCGTTGAGACAATTAATTTTCCGCCTGCTTTTTTATCAACGCCAGTTACAGTCCCGCCATCCATAACCTCCAGTAAGCCGCCACTATCTACAGTGGTATTATAAg                                                   <  1:154144/149‑1 (MQ=255)
                                                                                                    tAAATTGGCCTTTACTGTTTGGACCACTCACTTCCAGCGCATTCGTTGAGACAATTAATTTTCCGCCTGCTTTTTTATCAACGCCAGTTACAGTCCCGCCATCCATAACCTCCAGTAAGCCGCCACTATCTACAGTGGTATTATAAGCg                                                 >  4:10129/1‑149 (MQ=255)
                                                                                                             cTTTACTGTTTGGACCACTCACTTCCAGCGCATTCGTTGAGACAATTAATTTTCCGCCTGCTTTTTTATCAACGCCAGTTACAGTCCCGCCATCCATAACCTCCAGTAAGCCGCCACTATCTACAGTGGTATTATAAGCGAAGTCAttt                                       <  3:569277/149‑1 (MQ=255)
                                                                                                              tttACTGTTTGGACCACTCACTTCCAGCGCATTCGTTGAGACAATTAATTTTCCGCCTGCTTTTTTATCAACGCCAGTTACAGTCCCGCCATCCATAACCTCCAGTAAGCCGCCACTATCTACAGTGGTATTATAAGCGAAGTCAtttt                                      >  2:287989/1‑149 (MQ=255)
                                                                                                                 aCTGTTTGGACCACTCACTTCCAGCGCATTCGTTGAGACAATTAATTTTCCGCCTGCTTTTTTATCAACGCCAGTTACAGTCCCGCCATCCATAACCTCCAGTAAGCCGCCACTATCTACAGTGGTATTATAAGCGAAGTCATTttctt                                   <  4:284351/149‑1 (MQ=255)
                                                                                                                 aCTGTTTGGACCACTCACTTCCAGCGCATTCGTTGAGACAAGTAATTTTCCGCCTGCTTTTTTATCAACGCCAGTTGCAGTCCCGCCATCCATAACCTCCAGTAAGCCGCCACTATCTACAGTGGTATTATAAGCGAAGTCATTttctt                                   <  4:478489/149‑1 (MQ=255)
                                                                                                                           ccACTCACTTCCAGCGCATTCGTTGAGACAATTAATTTTCCGCCTGCTTTTTTATCAACGCCAGTTACAGTCCCGCCATCCATAACCTCCAGTAAGCCGCCACTATCTACAGTGGTATTATAAGCGAAGTCATTTTCTTCAACTCGCaa                         >  4:383219/1‑149 (MQ=255)
                                                                                                                           ccACTCACTTCCAACGCATTCGTCGAGACAATTAATTTTCCGCCTGCTTTTTTATCAACGCCAGTTACAGTCCCGCCATCCATAACCTCCAGTAAGCCGCCACTATCTACAGTGGTATTATAAGCGAAGTCa                                           <  2:300191/132‑1 (MQ=255)
                                                                                                                           ccACTCACTTCCAACGCATTCGTCGAGACAATTAATTTTCCGCCTGCTTTTTTATCAACGCCAGTTACAGTCCCGCCATCCATAACCTCCAGTAAGCCGCCACTATCTACAGTGGTATTATAAGCGAAGTCa                                           >  1:300191/1‑132 (MQ=255)
                                                                                                                               tcactTCCAGCGCATTCGTTGAGACAATTAATTTTCCGCCTGCTTTTTTATCAACGCCAGTTACAGTCCCGCCATCCATAACCTCCAGTAAGCCGCCACTATCTACAGTGGTATTATAAGCGAAGTCATTTTCTTCAACTCGCAAACtt                     >  3:473549/1‑149 (MQ=255)
                                                                                                                                    tCCAGCGCATTCGTTGAGACAATTAATTTTCCGCCTGCTTTTTTATCAACGCCAGTTACAGTCCCGCCATCCATAACCTCCAGTAAGCCGCCACTATCTACAGTGGTATTATAAGCGAAGTCATTTTCTTCAACTCGCAAACTTccgcc                <  4:426728/149‑1 (MQ=255)
                                                                                                                                    tCCAGCGCATTCGTTGAGACAATTAATTTTCCGCCTGCTTTTTTATCAACGCCAGTTACAGTCCCGCCATCCATAACCTCCAGTAAGCCGCCACTATCTACAGTGGTATTATAAGCGAAGTCATTTTCTTCAACTCGCAAACTTccgcc                >  2:434435/1‑149 (MQ=255)
                                                                                                                                      cAGCGCATTCGTTGAGACAATTAATTTTCCGCCTGCTTTTTTATCAACGCCAGTTACAGTCCCGCCATCCATAACCTCCAGTAAGCCGCCACTATCTACAGTGGTATTATAAGCGAAGTCATTTTTCTTCAACTCGCAAACTTccgccg               >  2:541468/1‑149 (MQ=255)
                                                                                                                                      cAGCGCATTCGTTGAGACAATTAATTTTCCGCCTGCTTTTTTATCAACGCCAGTTACAGTCCCGCCATCCATAACCTCCAGTAAGCCGCCACTATCTACAGTGGTATTATAAGCGAAGTCATTTTCTTCAACTCGCAAACTTCCGCCGt              >  1:243447/1‑149 (MQ=255)
                                                                                                                                        gcgcATTCGTTGAGACAATTAATTTTCCGCCTGCTTTTTTATCAACGCCAGTTACAGTCCCGCCATCCATAACCTCCAGTAAGCCGCCACTATCTACAGTGGTATTATAAGc                                                  >  4:271517/1‑112 (MQ=255)
                                                                                                                                        gcgcATTCGTTGAGACAATTAATTTTCCGCCTGCTTTTTTATCAACGCCAGTTACAGTCCCGCCATCCATAACCTCCAGTAAGCCGCCACTATCTACAGTGGTATTATAAGc                                                  <  3:271517/112‑1 (MQ=255)
                                                                                                                                         cgcATTCGTTGAGACAATTAATTTTCCGCCTGCTTTTTTATCAACGCCAGTTACAGTCCCGCCATCCATAACCTCCAGTAAGCCGCCACTATCTACAGTGGTATTATAAGCGAAGTCATTTTCTTCAACTCGCAAACTTCCGCCGtttt           <  1:490600/149‑1 (MQ=255)
                                                                                                                                                 ttGAGACAATTAATTTTCCGCCTGCTTTTTTATCAACGCCAGTTACAGTCCCGCCATCCATAACCTCCAGTAAGCCGCCACTATCTACAGTGGTATTATAAGCGAAGTCATTTTCTTCAACTCGCAAACTTCCGCCGTTTTCCaacaac   >  2:105461/1‑149 (MQ=255)
                                                                                                                                                  tGAGACAATTAATTTTCCGCCTGCTTTTTTATCAACGCCAGTTACAGTCCCGCCATCCATAACCTCCAGTAAGCCGCCACTATCTACAGTGGTATTATAAGCGAAGTCATTTTCTTCAACTCGCAAACTTCCGCCGTTTTCCAacaaca  >  3:129901/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
TGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATACTAAATTGGCCTTTACTGTTTGGACCACTCACTTCCAGCGCATTCGTTGAGACAATTAATTTTCCGCCTGCTTTTTTATCAACGCCAGTTACAGTCCCGCCATCCATAACCTCCAGTAAGCCGCCACTATCTACAGTGGTATTATAAGCGAAGTCATTTTCTTCAACTCGCAAACTTCCGCCGTTTTCCAACAACA  >  NC_000913/2288086‑2288380

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: