Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 238,141 G→A 7.5% intergenic (+21/+116) yafT → / ← ykfM lipoprotein/lethality reduction protein, putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913238,1410GA 7.5% 161.1 / 3.8 53intergenic (+21/+116)yafT/ykfMlipoprotein/lethality reduction protein, putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (23/26);  new base A (2/2);  total (25/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.23e-01

GTGCTTCCACCATTGCCCGACAAGAATGAAGCCAGTACGACTGATATGACAGAGCAGCAAATCATGCAACTTAAGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAGTGCAAGAAAGGACATGGAATCCGGAGTTAAAAACTGTTTGTTTTAGAATATTTAATTATTTAACTGCTGAATCTTCCTTGCGAGAA  >  NC_000913/237995‑238287
                                                                                                                                                  |                                                                                                                                                  
gTGCTTCCACCATTGCCCGACAAGAATGAAGCCAGTACGACTGATATGACAGAGCAGCAAATCATGCAACTTAAGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGaa                                                                                                                                                  >  4:129426/1‑149 (MQ=255)
       caccaTTGCCCGACAAGAATGAAGCCAGTACGACTGATATGACAGAGCAGCAAATCATGCAACTTAAGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTgg                                                                                                                                           <  2:273399/149‑1 (MQ=255)
        accaTTGCCCGACAAGAATGAAGCCAGTACGACTGATATGACAGAGCAGCAAATCATGCAACTTAAGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAgg                                                                                                                                                    <  2:67889/139‑1 (MQ=255)
        accaTTGCCCGACAAGAATGAAGCCAGTACGACTGATATGACAGAGCAGCAAATCATGCAACTTAAGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAgg                                                                                                                                                    >  1:67889/1‑139 (MQ=255)
        accaTTGCCCGACAAGAATGAAGCCAGTACGACTGATATGACAGAGCAGCAAATCATGCAACTTAAGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTggc                                                                                                                                          >  2:411627/1‑149 (MQ=255)
         ccaTTGCCCGACAAGAATGAAGCCAGTACGACTGATATGACAGAGCAGCAAATCATGCAACTTAAGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTggcg                                                                                                                                         >  2:109626/1‑149 (MQ=255)
                  gACAAGAATGAAGCCAGTACGACTGATATGACAGAGCAGCAAATCATGCAACTTAAGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAataata                                                                                                                                >  3:374421/1‑149 (MQ=255)
                   aCAAGAATGAAGCCAGTACGACTGATATGACAGAGCAGCAAATCATGCAACTTAAGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATAc                                                                                                                               >  4:332675/1‑149 (MQ=255)
                     aaGAATGAAGCCAGTACGACTGATATGACAGAGCAGCAAATCATGCAACTTAAGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACaa                                                                                                                             >  1:49310/1‑149 (MQ=255)
                     aaGAATGAAGCCAGTACGACTGATATGACAGAGCAGCAAATCATGCAACTTAAGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACaa                                                                                                                             >  4:356414/1‑149 (MQ=255)
                      aGAATGAAGCCAGTACGACTGATATGACAGAGCAGCAAATCATGCAACTTAAGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACaaa                                                                                                                            >  2:458689/1‑149 (MQ=255)
                         aTGAAGCCAGTACGACTGATATGACAGAGCAGCAAATCATGCAACTTAAGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAAtt                                                                                                                         >  1:4101/1‑149 (MQ=255)
                              gCCAGTACGACTGATATGACAGAGCAGCAAATCATGCAACTTAAGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTg                                                                                                                    >  1:360316/1‑149 (MQ=255)
                                   tACGACTGATATGACAGAGCAGCAAATCATGCAACTTAAGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATaa                                                                                                               >  2:428707/1‑149 (MQ=255)
                                    aCGACTGATATGACAGAGCAGCAAATCATGCAACTTAAGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAg                                                                                                              <  3:277283/149‑1 (MQ=255)
                                          gATATGACAGAGCAGCAAATCATGCAACTTAAGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAAttt                                                                                                        <  3:356414/149‑1 (MQ=255)
                                             atGACAGAGCAGCAAATCATGCAACTTAAGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTc                                                                                                     <  3:355994/149‑1 (MQ=255)
                                               gACAGAGCAGCAAATCATGCAACTTAAGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCtt                                                                                                   <  1:36525/149‑1 (MQ=255)
                                                 cAGAGCAGCAAATCATGCAACTTAAGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGa                                                                                                 <  2:340159/149‑1 (MQ=255)
                                                   gagCAGCAAATCATGCAACTTAAGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACa                                                                                               <  2:4101/149‑1 (MQ=255)
                                                          aaaTCATGCAACTTAAGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAg                                                                                        <  1:428707/149‑1 (MQ=255)
                                                          aaaTCATGCAACTTAAGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAg                                                                                        <  4:374421/149‑1 (MQ=255)
                                                                  cAACTTAAGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAGTGCAAGaa                                                                                >  1:78871/1‑149 (MQ=255)
                                                                   aaCTTAAGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAGTGCAAGaaa                                                                               >  1:327726/1‑149 (MQ=255)
                                                                   aaCTTAAGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAGTGCAAGaaa                                                                               >  2:261860/1‑149 (MQ=255)
                                                                   aaCTTAAGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAGTGCAAGaaa                                                                               <  2:78871/149‑1 (MQ=255)
                                                                    aCTTAAGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAGTGCAAGAAAg                                                                              <  2:284051/149‑1 (MQ=255)
                                                                    aCTTAAGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAGTGCAAGAAAg                                                                              <  1:493095/149‑1 (MQ=255)
                                                                      ttAAGCAAAAGCCCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAGTGCAAGAAAGGa                                                                            <  2:360316/149‑1 (MQ=255)
                                                                         aGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAGTGCAAGAAAGGACAt                                                                         <  4:341815/149‑1 (MQ=255)
                                                                          gCAAAAGACCTATAAAGCGATGATAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAGTGCAGGAAAGGACATg                                                                        <  3:332675/149‑1 (MQ=255)
                                                                            aaaaGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAGTGCAAGAAAg                                                                              >  4:281866/1‑141 (MQ=255)
                                                                            aaaaGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAGTGCAAGAAAg                                                                              <  3:281866/141‑1 (MQ=255)
                                                                            aaaaGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAGTGCAAGAAAGGACATGGa                                                                      >  4:59450/1‑149 (MQ=255)
                                                                              aaGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAGTGCAAGAAAGGACATGGAAt                                                                    >  3:540034/1‑149 (MQ=255)
                                                                                   cTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAGTGCAAGAAAGGACATGGAATCCGGa                                                               <  1:458689/149‑1 (MQ=255)
                                                                                    tataAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAGTGCAAGAAAGGACATGGAATCCGGAg                                                              >  3:420981/1‑149 (MQ=255)
                                                                                               ggTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAGTGCAAGAAAGGACATGGAATCCGGAGTTAAAAACtgt                                                   <  1:411627/149‑1 (MQ=255)
                                                                                                gTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAGTGCAAGAAAGGACATGGAATCCGGAGTTAAAAACtgtt                                                  <  4:420981/149‑1 (MQ=255)
                                                                                                 tAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAGTGCAAGAAAGGACATGGAATCCGGAGTTAAAAACtgttt                                                 <  1:109626/149‑1 (MQ=255)
                                                                                                  aaaaGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGAAATTCCTGGCGGCagat                                                                                                                                   >  1:207223/1‑63 (MQ=37)
                                                                                                  aaaaGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGAAATTCCTGGCGGCagat                                                                                                                                   <  2:207223/66‑4 (MQ=37)
                                                                                                   aaaGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAGTGCAAGAAAGGACATGGAATCCGGAGTTAAAAACtgtttgt                                               >  3:25562/1‑149 (MQ=255)
                                                                                                    aaGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAGTGCAAGAAAGGACATGGAATCCGGAGTTAAAAACtgtttgtt                                              <  3:377311/149‑1 (MQ=255)
                                                                                                     aGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAGTGCAAGAAAGGACATGGAATCCGGAGTTAAAAACTgtttgttt                                             <  2:389308/149‑1 (MQ=255)
                                                                                                             ttAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCtt                                                                                                   <  3:565145/87‑1 (MQ=255)
                                                                                                             ttAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCtt                                                                                                   >  4:565145/1‑87 (MQ=255)
                                                                                                                 ccGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAGTGCAAGAAAGGACATGGAATCCGGAGTTAAAAACTGTTTGTTTTAGAATATTTaa                                 <  2:542966/149‑1 (MQ=255)
                                                                                                                         aataatAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAGTGCAAGAAAGGACATGGAATCCGGAGTTAAAAACTGTTTGTTTTAGAATATTTAATTATTTaa                         <  2:189849/149‑1 (MQ=255)
                                                                                                                                          gccgccAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAGTGCAAGAAAGGACATGGAATCCGGAGTTAAAAACTGTTTGTTTTAGAATATTTaa                                 >  3:304716/1‑124 (MQ=255)
                                                                                                                                          gccgccAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAGTGCAAGAAAGGACATGGAATCCGGAGTTAAAAACTGTTTGTTTTAGAATATTTaa                                 <  4:304716/124‑1 (MQ=255)
                                                                                                                                          gccgccAGAAATTCCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAGTGCAAGAAAgg                                                                             <  2:4452/80‑1 (MQ=39)
                                                                                                                                          gccgccAGAAATTCCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAGTGCAAGAAAgg                                                                             >  1:4452/1‑80 (MQ=39)
                                                                                                                                              ccAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAGTGCAAGAAAGGACATGGAATCCGGAGTTAAAAACTGTTTGTTTTAGAATATTTAATTATTTAACTGCTGAATCTTCCTTGCgag    >  3:292661/1‑149 (MQ=255)
                                                                                                                                                aGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAGTGCAAGAAAGGACATGGAATCCGGAGTTAAAAACTGTTTGTTTTAGAATATTTAATTATTTAACTGCTGAATCTTCCTTGCGAGaa  >  2:396377/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GTGCTTCCACCATTGCCCGACAAGAATGAAGCCAGTACGACTGATATGACAGAGCAGCAAATCATGCAACTTAAGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAGTGCAAGAAAGGACATGGAATCCGGAGTTAAAAACTGTTTGTTTTAGAATATTTAATTATTTAACTGCTGAATCTTCCTTGCGAGAA  >  NC_000913/237995‑238287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑