Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 238,146 T→C 7.7% intergenic (+26/+111) yafT → / ← ykfM lipoprotein/lethality reduction protein, putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913238,1460TC 7.7% 153.1 / 4.4 52intergenic (+26/+111)yafT/ykfMlipoprotein/lethality reduction protein, putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (22/26);  new base C (2/2);  total (24/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.96e-01

CACCATTGCCCGACAAGAATGAAGCCAGTACGACTGATATGACAGAGCAGCAAATCATGCAACTTAAGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAGTGCAAGAAAGGACATGGAATCCGGAGTTAAAAACTGTTTGTTTTAGAATATTTAATTATTTAACTGCTGAATCTTCCTTGCGAGAAATATA  >  NC_000913/238002‑238292
                                                                                                                                                |                                                                                                                                                  
caccaTTGCCCGACAAGAATGAAGCCAGTACGACTGATATGACAGAGCAGCAAATCATGCAACTTAAGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTgg                                                                                                                                                <  2:273399/149‑1 (MQ=255)
 accaTTGCCCGACAAGAATGAAGCCAGTACGACTGATATGACAGAGCAGCAAATCATGCAACTTAAGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTggc                                                                                                                                               >  2:411627/1‑149 (MQ=255)
  ccaTTGCCCGACAAGAATGAAGCCAGTACGACTGATATGACAGAGCAGCAAATCATGCAACTTAAGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTggcg                                                                                                                                              >  2:109626/1‑149 (MQ=255)
           gACAAGAATGAAGCCAGTACGACTGATATGACAGAGCAGCAAATCATGCAACTTAAGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAataata                                                                                                                                     >  3:374421/1‑149 (MQ=255)
            aCAAGAATGAAGCCAGTACGACTGATATGACAGAGCAGCAAATCATGCAACTTAAGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATAc                                                                                                                                    >  4:332675/1‑149 (MQ=255)
              aaGAATGAAGCCAGTACGACTGATATGACAGAGCAGCAAATCATGCAACTTAAGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACaa                                                                                                                                  >  1:49310/1‑149 (MQ=255)
              aaGAATGAAGCCAGTACGACTGATATGACAGAGCAGCAAATCATGCAACTTAAGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACaa                                                                                                                                  >  4:356414/1‑149 (MQ=255)
               aGAATGAAGCCAGTACGACTGATATGACAGAGCAGCAAATCATGCAACTTAAGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACaaa                                                                                                                                 >  2:458689/1‑149 (MQ=255)
                  aTGAAGCCAGTACGACTGATATGACAGAGCAGCAAATCATGCAACTTAAGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAAtt                                                                                                                              >  1:4101/1‑149 (MQ=255)
                       gCCAGTACGACTGATATGACAGAGCAGCAAATCATGCAACTTAAGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTg                                                                                                                         >  1:360316/1‑149 (MQ=255)
                            tACGACTGATATGACAGAGCAGCAAATCATGCAACTTAAGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATaa                                                                                                                    >  2:428707/1‑149 (MQ=255)
                             aCGACTGATATGACAGAGCAGCAAATCATGCAACTTAAGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAg                                                                                                                   <  3:277283/149‑1 (MQ=255)
                                   gATATGACAGAGCAGCAAATCATGCAACTTAAGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAAttt                                                                                                             <  3:356414/149‑1 (MQ=255)
                                      atGACAGAGCAGCAAATCATGCAACTTAAGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTc                                                                                                          <  3:355994/149‑1 (MQ=255)
                                        gACAGAGCAGCAAATCATGCAACTTAAGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCtt                                                                                                        <  1:36525/149‑1 (MQ=255)
                                          cAGAGCAGCAAATCATGCAACTTAAGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGa                                                                                                      <  2:340159/149‑1 (MQ=255)
                                            gagCAGCAAATCATGCAACTTAAGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACa                                                                                                    <  2:4101/149‑1 (MQ=255)
                                                   aaaTCATGCAACTTAAGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAg                                                                                             <  4:374421/149‑1 (MQ=255)
                                                   aaaTCATGCAACTTAAGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAg                                                                                             <  1:428707/149‑1 (MQ=255)
                                                           cAACTTAAGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAGTGCAAGaa                                                                                     >  1:78871/1‑149 (MQ=255)
                                                            aaCTTAAGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAGTGCAAGaaa                                                                                    >  1:327726/1‑149 (MQ=255)
                                                            aaCTTAAGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAGTGCAAGaaa                                                                                    >  2:261860/1‑149 (MQ=255)
                                                            aaCTTAAGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAGTGCAAGaaa                                                                                    <  2:78871/149‑1 (MQ=255)
                                                             aCTTAAGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAGTGCAAGAAAg                                                                                   <  2:284051/149‑1 (MQ=255)
                                                             aCTTAAGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAGTGCAAGAAAg                                                                                   <  1:493095/149‑1 (MQ=255)
                                                               ttAAGCAAAAGCCCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAGTGCAAGAAAGGa                                                                                 <  2:360316/149‑1 (MQ=255)
                                                                  aGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAGTGCAAGAAAGGACAt                                                                              <  4:341815/149‑1 (MQ=255)
                                                                   gCAAAAGACCTATAAAGCGATGATAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAGTGCAGGAAAGGACATg                                                                             <  3:332675/149‑1 (MQ=255)
                                                                     aaaaGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAGTGCAAGAAAg                                                                                   <  3:281866/141‑1 (MQ=255)
                                                                     aaaaGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAGTGCAAGAAAg                                                                                   >  4:281866/1‑141 (MQ=255)
                                                                     aaaaGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAGTGCAAGAAAGGACATGGa                                                                           >  4:59450/1‑149 (MQ=255)
                                                                       aaGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAGTGCAAGAAAGGACATGGAAt                                                                         >  3:540034/1‑149 (MQ=255)
                                                                            cTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAGTGCAAGAAAGGACATGGAATCCGGa                                                                    <  1:458689/149‑1 (MQ=255)
                                                                             tataAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAGTGCAAGAAAGGACATGGAATCCGGAg                                                                   >  3:420981/1‑149 (MQ=255)
                                                                                        ggTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAGTGCAAGAAAGGACATGGAATCCGGAGTTAAAAACtgt                                                        <  1:411627/149‑1 (MQ=255)
                                                                                         gTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAGTGCAAGAAAGGACATGGAATCCGGAGTTAAAAACtgtt                                                       <  4:420981/149‑1 (MQ=255)
                                                                                          tAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAGTGCAAGAAAGGACATGGAATCCGGAGTTAAAAACtgttt                                                      <  1:109626/149‑1 (MQ=255)
                                                                                           aaaaGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGAAATTCCTGGCGGCagat                                                                                                                                        >  1:207223/1‑63 (MQ=37)
                                                                                           aaaaGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGAAATTCCTGGCGGCagat                                                                                                                                        <  2:207223/66‑4 (MQ=37)
                                                                                            aaaGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAGTGCAAGAAAGGACATGGAATCCGGAGTTAAAAACtgtttgt                                                    >  3:25562/1‑149 (MQ=255)
                                                                                             aaGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAGTGCAAGAAAGGACATGGAATCCGGAGTTAAAAACtgtttgtt                                                   <  3:377311/149‑1 (MQ=255)
                                                                                              aGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAGTGCAAGAAAGGACATGGAATCCGGAGTTAAAAACTgtttgttt                                                  <  2:389308/149‑1 (MQ=255)
                                                                                                      ttAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCtt                                                                                                        <  3:565145/87‑1 (MQ=255)
                                                                                                      ttAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCtt                                                                                                        >  4:565145/1‑87 (MQ=255)
                                                                                                          ccGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAGTGCAAGAAAGGACATGGAATCCGGAGTTAAAAACTGTTTGTTTTAGAATATTTaa                                      <  2:542966/149‑1 (MQ=255)
                                                                                                                  aataatAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAGTGCAAGAAAGGACATGGAATCCGGAGTTAAAAACTGTTTGTTTTAGAATATTTAATTATTTaa                              <  2:189849/149‑1 (MQ=255)
                                                                                                                                   gccgccAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAGTGCAAGAAAGGACATGGAATCCGGAGTTAAAAACTGTTTGTTTTAGAATATTTaa                                      >  3:304716/1‑124 (MQ=255)
                                                                                                                                   gccgccAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAGTGCAAGAAAGGACATGGAATCCGGAGTTAAAAACTGTTTGTTTTAGAATATTTaa                                      <  4:304716/124‑1 (MQ=255)
                                                                                                                                   gccgccAGAAATTCCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAGTGCAAGAAAgg                                                                                  <  2:4452/80‑1 (MQ=39)
                                                                                                                                   gccgccAGAAATTCCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAGTGCAAGAAAgg                                                                                  >  1:4452/1‑80 (MQ=39)
                                                                                                                                       ccAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAGTGCAAGAAAGGACATGGAATCCGGAGTTAAAAACTGTTTGTTTTAGAATATTTAATTATTTAACTGCTGAATCTTCCTTGCgag         >  3:292661/1‑149 (MQ=255)
                                                                                                                                         aGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAGTGCAAGAAAGGACATGGAATCCGGAGTTAAAAACTGTTTGTTTTAGAATATTTAATTATTTAACTGCTGAATCTTCCTTGCGAGaa       >  2:396377/1‑149 (MQ=255)
                                                                                                                                              tttCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAGTGCAAGAAAGGACATGGAATCCGGAGTTAAAAACTGTTTGTTTTAGAATATTTAATTATTTAACTGCTGAATCTTCCTTGCGAGAAatata  >  3:245917/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
CACCATTGCCCGACAAGAATGAAGCCAGTACGACTGATATGACAGAGCAGCAAATCATGCAACTTAAGCAAAAGACCTATAAAGCGATGGTAAAAGATTTGGTTAACCGCTATCAATAATAAATTATATCTGCCGCCAGGAATTTCTGGCGGCAATAATACAAAATTCTTTGCATAAGAAATTTTTCTTGACAAAGACAGTGCAAGAAAGGACATGGAATCCGGAGTTAAAAACTGTTTGTTTTAGAATATTTAATTATTTAACTGCTGAATCTTCCTTGCGAGAAATATA  >  NC_000913/238002‑238292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑