Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,328,157 T→A 5.6% L547F (TTA→TTT)  yfaQ ← tandem DUF2300 domain protein, putative host defense protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,328,1570TA 5.6% 249.8 / 4.6 72L547F (TTA→TTT) yfaQtandem DUF2300 domain protein, putative host defense protein
Reads supporting (aligned to +/- strand):  ref base T (37/31);  new base A (2/2);  total (39/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.59e-01

TGAAAAACTGGCATCTTCGCCCTCTGCCGGATGCCAGCCTGAGAGCGGTGCATCAATCTCGACTTCCTGCACACCTTCACTGTGAGCGACAGGCGAAAGCGCCACCAGTAACAACGGAAGAAAAATCTTTCGCATAGCATTAGTCCATTAATAATTGTCGGGTCAGCGTTTCGATATAGTTCTCATCAAGCCCGGTGGGATAACCATCAAAGGCCAGGTGCAGATACTCATGGGTTAAATCCAGCCGATCCTGAAGCGTAAAGAAGTTACGAATAAACAGCCGTTTCTGCTGCCGA  >  NC_000913/2328009‑2328304
                                                                                                                                                    |                                                                                                                                                   
tgAAAAACTGGCATCTTCGCCCTCTGCCGGATGCCAGCCTGAGAGCGGTGCATCAATCTCGACTTCCTGCACACCTTCACTGTGAGCGACAGGCGAAAGCGCCACCAGTAACAACGGAAGAAAAATCTTTCGCATAGCATTAGTCCAtt                                                                                                                                                     >  4:579507/1‑149 (MQ=255)
  aaaaaCTGGCATCTTCGCCCTCTGCCGGATGCCAGCCTGAGAGCGGTGCATCAATCTCGACTTCCTGCACACCTTCACTGTGAGCGACAGGCGAAAGCGCCACCAGTAACAACGGAAGAAAAATCTTTCGCATAGCATTAGTCCATtaa                                                                                                                                                   >  3:465253/1‑149 (MQ=255)
   aaaaCTGGCATCTTCGCCCTCTGCCGGATGCCAGCCTGAGAGCGGTGCATCAATCTCGACTTCCTGCACACCTTCACTGTGAGCGACAGGCGAAAGCGCCACCAGTAACAACGGAAGAAAAATCTTTCGCATAGCATTAGTCCATtaat                                                                                                                                                  >  3:241154/1‑149 (MQ=255)
     aaCTGGCATCTTCGCCCTCTGCCGGATGCCAGCCTGAGAGCGGTGCATCAATCTCGACTTCCTGCACACCTTCACTGTGAGCGACAGGCGAAAGCGCCACCAGTAACAACGGAAGAAAAATCTTTCGCATAGCATTAGTCCATtaataa                                                                                                                                                >  2:19872/1‑149 (MQ=255)
        tGGCATCTTCGCCCTCTGCCGGATGCCAGCCTGAGAGCGGTGCATCAATCTCGACTTCCTGCACACCTTCACTGTGAGCGACAGGCGAAAGCGCCACCAGTAACAACGGAAGAAAAATCTTTCGCATAGCATTAGTCCATAAATAATTg                                                                                                                                             >  1:558307/1‑149 (MQ=255)
        tGGCATCTTCGCCCTCTGCCGGATGCCAGCCTGAGAGCGGTGCATCAATCTCGACTTCCTGCACACCTTCACTGTGAGCGACAGGCGAAAGCGCCACCAGTAACAACGGAAGAAAAATCTTTCGCATAGCATTAGTCCATAAATAATTg                                                                                                                                             >  3:56401/1‑149 (MQ=255)
         ggCATCTTCGCCCTCTGCCGGATGCCAGCCTGAGAGCGGTGCATCAATCTCGACTTCCTGCACACCTTCACTGTGAGCGACAGGCGAAAGCGCCACCAGTAACAACGGAAGAAAAATCTTTCGCATAGCATTAGTCCATTAATAATTGt                                                                                                                                            <  3:225279/149‑1 (MQ=255)
           cATCTTCGCCCTCTGCCGGATGCCAGCCTGAGAGCGGTGCATCAATCTCGACTTCCTGCACACCTTCACTGTGAGCGACAGGCGAAAGCGCCACCAGTAACAACGGAAGAAAAATCTTTCGCATAGCATTAGTCCATTAATAATTGTCg                                                                                                                                          >  3:286766/1‑149 (MQ=255)
            aTCTTCGCCCTCTGCCGGATGCCAGCCTGAGAGCGGTGCATCAATCTCGACTTCCTGCACACCTTCACTGTGAGCGACAGGCGAAAGCGCCACCAGTAACAACGGAAGAAAAATCTTTCGCATAGCATTAGTCCATTAATAATTGTCgg                                                                                                                                         >  1:400878/1‑149 (MQ=255)
               ttCGCCCTCTGCCGGATGCCAGCCTGAGAGCGGTGCATCAATCTCGACTTCCTGCACACCTTCACTGTGAGCGACAGGCGAAAGCGCCACCAGTAACAACGGAAGAAAAATCTTTCGCATAGCATTAGTCCATTAATAATTGTCGGGTc                                                                                                                                      >  4:525358/1‑149 (MQ=255)
                  gCCCTCTGCCGGATGCCAGCCTGAGAGCGGTGCATCAATCTCGACTTCCTGCACACCTTCACTGTGAGCGACAGGCGAAAGCGCCACCAGTAACAACGGAAGAAAAATCTTTCGCATAGCATTAGTCCATTAATAATTGTCGGGTCAGc                                                                                                                                   >  3:102036/1‑149 (MQ=255)
                    ccTCTGCCGGATGCCAGCCTGAGAGCGGTGCATCAATCTCGACTTCCTGCACACCTTCACTGTGAGCGACAGGCGAAAGCGCCACCAGTAACAACGGAAGAAAAATCTTTCGCATAGCATTAGTCCATTAATAATTGTCGGGTCAGCGt                                                                                                                                 >  1:130471/1‑149 (MQ=255)
                                gCCAGCCTGAGAGCGGTGCATCAATCTCGACTTCCTGCACACCTTCACTGTGAGCGACAGGCGAAAGCGCCACCAGTAACAACGGAAGAAAAATCTTTCGCATAGCATTAGTCCATTAATAATTGTCGGGTCAGCGTTTCGATATAGtt                                                                                                                     <  4:285315/149‑1 (MQ=255)
                                  cAGCCTGAGAGCGGTGCATCAATCTCGACTTCCTGCACACCTTCACTGTGAGCGACAGGCGAAAGCGCCACCAGTAACAACGGAAGAAAAATCTTTCGCATAGCATTAGTCCATTAATAATTGTCGGGTCAGCGTTTCGATATAGTtct                                                                                                                   >  1:165175/1‑149 (MQ=255)
                                   aGCCTGAGAGCGGTGCATCAATCTCGACTTCCTGCACACCTTCACTGTGAGCGACAGGCGAAAGCGCCACCAGTAACAACGGAAGAAAAATCTTTCGCATAGCATTAGTCCATTAATAATTGTCGGGTCAGCGTTTCGATATAGTtctc                                                                                                                  >  4:279283/1‑149 (MQ=255)
                                   aGCCTGAGAGCGGTGCATCAATCTCGACTTCCTGCACACCTTCACTGTGAGCGACAGGCGAAAGCGCCACCAGTAACAACGGAAGAAAAATCTTTCGCATAGCATTAGTCCATTAATAATTGTCGGGTCAGCGTTTCGATATAGTtctc                                                                                                                  >  1:30463/1‑149 (MQ=255)
                                    gCCTGAGAGCGGTGCATCAATCTCGACTTCCTGCACACCTTCACTGTGAGCGACAGGCGAAAGCGCCACCAGTAACAACGGAAGAAAAATCTTTCGCATAGCATTAGTCCATTAATAATTGTCGGGTCAGCGTTTCGATATAGTTCtca                                                                                                                 >  3:304741/1‑149 (MQ=255)
                                               gTGCATCAATCTCGACTTCCTGCACACCTTCACTGTGAGCGACAGGCGAAAGCGCCACCAGTAACAACGGAAGAAAAATCTTTCGCATAGCATTAGTCCATTAATAATTGTCGGGTCAGCGTTTCGATATAGTTCTCATCAAGCCCGGt                                                                                                      <  1:161261/149‑1 (MQ=255)
                                               gTGCATCAATCTCGACTTCCTGCACACCTTCACTGTGAGCGACAGGCGAAAGCGCCACCAGTAACAACGGAAGAAAAATCTTTCGCATAGCATTAGTCCATTAATAATTGTCGGGTCAGCGTTTCGATATAGTTCTCATCAAGCCCGGt                                                                                                      >  1:141892/1‑149 (MQ=255)
                                                tGCATCAATCTCGACTTCCTGCACACCTTCACTGTGAGCGACAGGCGAAAGCGCCACCAGTAACAACGGAAGAAAAATCTTTCGCATAGCATTAGTCCATTAATAATTGTCg                                                                                                                                          <  3:198001/112‑1 (MQ=255)
                                                tGCATCAATCTCGACTTCCTGCACACCTTCACTGTGAGCGACAGGCGAAAGCGCCACCAGTAACAACGGAAGAAAAATCTTTCGCATAGCATTAGTCCATTAATAATTGTCg                                                                                                                                          >  4:198001/1‑112 (MQ=255)
                                                tGCATCAATCTCGACTTCCTGCACACCTTCACTGTGAGCGACAGGCGAAAGCGCCACCAGTAACAACGGAAGAAAAATCTTTCGCATAGCATTAGTCCATTAATAATTGTCGGGTCAGCGTTTCGATATAGTTCTCATCAAGCCCGGTg                                                                                                     <  2:342445/149‑1 (MQ=255)
                                                tGCATCAATCTCGACTTCCTGCACACCTTCACTGTGAGCGACAGGCGAAAGCGCCACCAGTAACAACGGAAGAAAAATCTTTCGCATAGCATTAGTCCATTAATAATTGTCGGGTCAGCGTTTCGATATAGTTCTCATCAAGCCCGGTg                                                                                                     <  4:465253/149‑1 (MQ=255)
                                                        tctcGACTTCCTGCACACCTTCACTGTGAGCGACAGGCGAAAGCGCCACCAGTAACAACGGAAGAAAAATCTTTCGCATAGCATTAGTCCATTAATAATTGTCGGGTCAGCGTTTCGATATAGTTCTCATCAAGCCCGGTGGGATAAcc                                                                                             <  3:402152/149‑1 (MQ=255)
                                                        tctcGACTTCCTGCACACCTTCACTGTGAGCGACAGGCGAAAGCGCCACCAGTAACAACGGAAGAAAAATCTTTCGCATAGCATTAGTCCATTAATAATTGTCGGGTCAGCGTTTCGATATAGTTCTCATCAAGCCCGGTGGGATAAcc                                                                                             <  4:102036/149‑1 (MQ=255)
                                                          tcGACTTCCTGCACACCTTCACTGTGAGCGACAGGCGAAAGCGCCACCAGTAACAACGGAAGAAAAATCTTTCGCATAGCATTAGTCCATTAATAATTGTCGGGTCAGCGTTTCGAtata                                                                                                                        <  2:220697/120‑1 (MQ=255)
                                                          tcGACTTCCTGCACACCTTCACTGTGAGCGACAGGCGAAAGCGCCACCAGTAACAACGGAAGAAAAATCTTTCGCATAGCATTAGTCCATTAATAATTGTCGGGTCAGCGTTTCGAtata                                                                                                                        >  1:220697/1‑120 (MQ=255)
                                                          tcGACTTCCTGCACACCTTCACTGTGAGCGACAGGCGAAAGCGCCACCAGTAACAACGGAAGAAAAATCTTTCGCATAGCATTAGTCCATTAATAATTGTCGGGTCAGCGTTTCGATATAGTTCTCATCAAGCCCGGTGGGATAACCAt                                                                                           >  3:380976/1‑149 (MQ=255)
                                                           cGACTTCCTGCACACCTTCACTGTGAGCGACAGGCGAAAGCGCCACCAGTAACAACGGAAGAAAAATCTTTCGCATAGCATTAGTCCATTAATAATTGTc                                                                                                                                           >  1:372402/1‑100 (MQ=255)
                                                           cGACTTCCTGCACACCTTCACTGTGAGCGACAGGCGAAAGCGCCACCAGTAACAACGGAAGAAAAATCTTTCGCATAGCATTAGTCCATTAATAATTGTc                                                                                                                                           <  2:372402/100‑1 (MQ=255)
                                                           cGACTTCCTGCACACCTTCACTGTGAGCGACAGGCGAAAGCGCCACCAGTAACAACGGAAGAAAAATCTTTCGCATAGCATTAGTCCATTAATAATTGTCGGGTCAGCGTTTCGATATAGTTCTCATCAAGCCCGGTGGGATAACCATc                                                                                          >  2:384776/1‑149 (MQ=255)
                                                              cTTCCTGCACACCTTCACTGTGAGCGACAGGCGAAAGCGCCACCAGTAACAACGGAAGAAAAATCTTTCGCATAGCATTAGTCCATTAATAATTGTCGGGTCAGCGTTTCGATATAGTTCTCATCAAGCCCGGTGGGATAACCATCaaa                                                                                       <  3:254605/149‑1 (MQ=255)
                                                               ttCCTGCACACCTTCACTGTGAGCGACAGGCGAAAGCGCCACCAGTAACAACGGAAGAAAAATCTTTCGCATAGCATTAGTCCATTAATAATTGTCGGGTCAGCGTTTCGATATAGTTCTCATCAAGCCCGGTGGGATAACCATCAAAg                                                                                      <  4:286766/149‑1 (MQ=255)
                                                               ttCCTGCACACCTTCACTGTGAGCGACAGGCGAAAGCGCCACCAGTAACAACGGAAGAAAAATCTTTCGCATAGCATTAGTCCATTAATAATTGTCGGGTCAGCGTTTCGATATAGTTCTCATCAAGCCCGGTGGGATAACCATCAAAg                                                                                      <  2:141892/149‑1 (MQ=255)
                                                                  cTGCACACCTTCACTGTGAGCGACAGGCGAAAGCGCCACCAGTAACAACGGAAGAAAAATCTTTCGCATAGCATTAGTCCATTAATAATTGTCGGGTCAGCGTTTCGATATAGTTCTCATCAAGCCCGGTGGGATAACCATCAAAGGcc                                                                                   >  1:505592/1‑149 (MQ=255)
                                                                    gCACACCTTCACTGTGAGCGACAGGCGAAAGCGCCACCAGTAACAACGGAAGAAAAATCTTTCGCATAGCATTAGTCCATTAATAATTGTCGGGTCAGCGTTTCGATATAGTTCTCATCAAGCCCGGTGGGATAACCATCAAAGGCCAg                                                                                 <  1:184644/149‑1 (MQ=255)
                                                                        acCTTCACTGTGAGCGACAGGCGAAAGCGCCACCAGTAACAACGGAAGAAAAATCTTTCGCATAGCATTAGTCCATTAATAATTGTCGGGTCAGCGTTTCGATATAGTTCTCATCAAGCCCGGTGGGATAACCATCAAAGGCCAGGTGc                                                                             >  1:56589/1‑149 (MQ=255)
                                                                        acCTTCACTGTGAGCGACAGGCGAAAGCGCCACCAGTAACAACGGAAGAAAAATCTTTCGCATAGCATTAGTCCATTAATAATTGTCGGGTCAGCGTTTCGATATAGTTCTCATCAAGCCCGGTGGGATAACCATCAAAGGCCAGGTGc                                                                             >  2:496984/1‑149 (MQ=255)
                                                                          cTTCACTGTGAGCGACAGGCGAAAGCGCCACCAGTAACAACGGAAGAAAAATCTTTCGCATAGCATTAGTCCATTAATAATTGTCGGGTCAGCGTTTCGATATAGTTCTCATCAAGCCCGGTGGGATAACCATCAAAGGCCAGGTGCAg                                                                           >  2:412748/1‑149 (MQ=255)
                                                                          cTTCACTGTGAGCGACAGGCGAAAGCGCCACCAGTAACAACGGAAGAAAAATCTTTCGCATAGCATTAGTCCATTAATAATTGTCGGGTCAGCGTTTCGATATAGTTCTCATCAAGCCCGGTGGGATAACCATCAAAGGCCAGGTGCAg                                                                           >  3:554351/1‑149 (MQ=255)
                                                                            tCACTGTGAGCGACAGGCGAAAGCGCCACCAGTAACAACGGAAGAAAAATCTTTCGCATAGCATTAGTCCATTAATAATTGTCGGGTCAGCGTTTCGATATAGTTCTCATCAAGCCCGGTGGGATAACCATCAAAGGCCAGGTGCAGAt                                                                         >  3:508015/1‑149 (MQ=255)
                                                                                  tgAGCGACAGGCGAAAGCGCCACCAGTAACAACGGAAGAAAAATCTTTCGCATAGCATTAGTCCATTAATAATTGTCGGGTCAGCGTTTCGATATAGTTCTCATCAAGCCCGGTGGGATAACCATCAAAGGCCAGGTGCAGATACTCAt                                                                   >  3:232364/1‑149 (MQ=255)
                                                                                    aGCGACAGGCGAAAGCGCCACCAGTAACAACGGAAGAAAAATCTTTCGCATAGCATTAGTCCATTAATAATTGTCGGGTCAGCGTTTCGATATAGTTCTCATCAAGCCCGGTGGGATAACCATCAAAGGCCAGGTGCAGATACTCATgg                                                                 <  2:400878/149‑1 (MQ=255)
                                                                                       gACAGGCGAAAGCGCCACCAGTAACAACGGAAGAAAAATCTTTCGCATAGCATTAGTCCATTAATAATTGTCGGGTCAGCGTTTCGATATAGTTCTCATCAAGCCCGGTGGGATAACCATCAAAGGCCAGGTGCAGATACTCATGGGtt                                                              <  1:468447/149‑1 (MQ=255)
                                                                                          aGGCGAAAGCGCCACCAGTAACAACGGAAGAAAAATCTTTCGCATAGCATTAGTCCATTAATAATTGTCGGGTCAGCGTTTCGATATAGTTCTCATCAAGCCCGGTGGGATAACCATCAAAGGCCAGGTGCAGATACTCATGGGTTaaa                                                           <  4:224067/149‑1 (MQ=255)
                                                                                          aGGCGAAAGCGCCACCAGTAACAACGGAAGAAAAATCTTTCGCATAGCATTAGTCCATTAATAATTGTCGGGTCAGCGTTTCGATATAGTTCTCATCAAGCCCGGTGGGATAACCATCAAAGGCCAGGTGCAGATACTCATGGGTTaaa                                                           >  3:309548/1‑149 (MQ=255)
                                                                                             cGAAAGCGCCACCAGTAACAACGGAAGAAAAATCTTTCGCATAGCATTAGTCCATTAATAATTGTCGGGTCAGCGTTTCGATATAGTTCTCATCAAGCCCGGTGGGATAACCATCAAAGGCCAGGTGCAGATACTCATGGGTTAAATcc                                                        <  3:279283/149‑1 (MQ=255)
                                                                                              gAAAGCGCCACCAGTAACAACGGAAGAAAAATCTTTCGCATAGCATTAGTCCATtaa                                                                                                                                                   >  3:175792/1‑57 (MQ=255)
                                                                                              gAAAGCGCCACCAGTAACAACGGAAGAAAAATCTTTCGCATAGCATTAGTCCATtaa                                                                                                                                                   <  4:175792/57‑1 (MQ=255)
                                                                                               aaaGCGCCACCAGTAACAACGGAAGAAAAATCTTTCGCATAGCATTAGTCCATTAATAATTGTCGGGTCAGCGTTTCGATATAGTTCTCATCAAGCCCGGTGGGATAACCATCAAAGGCCAGGTGCAGATACTCATGGGTTAAATCCAg                                                      <  2:130471/149‑1 (MQ=255)
                                                                                                 aGCGCCACCAGTAACAACGGAAGAAAAATCTTTCGCATAGCATTAGTCCATTAATAATTGTCGGGTCAGCGTTTCGATATAGTTCTCATCAAGCCCGGTGGGATAACCATCAAAGGCCAGGTGCAGATACTCATGGGTTAAATCCAGcc                                                    <  3:525358/149‑1 (MQ=255)
                                                                                                    gcCACCAGTAACAACGGAAGAAAAATCTTTCGCATAGCATTAGTCCATTAATAATTGTCGGGTCAGCGTTTCGATATAGTTCTCATCAAGCCCGGTGGGATAACCATCAAAGGCCAGGTGCAGATACTCATGGGTTAAATCCAGCCGAt                                                 <  1:496984/149‑1 (MQ=255)
                                                                                                     ccaccaGTAACAACGGAAGAAAAATCTTTCGCATAGCATTAGTCCATTAATAATTGTCGGGTCAGCGTTTCGATATAGTTCTCATCAAGCCCGGTGGGATAACCATCAAAGGCCAGGTGCAGATACTCATGGGTTAAATCCAGCCGATc                                                >  2:157883/1‑149 (MQ=255)
                                                                                                       accaGTAACAACGGAAGAAAAATCTTTCGCATAGCATTAGTCCATTAATAATTGTCGGGTCAGCGTTTCGATATAGTTCTCATCAAGCCCGGTGGGATAACCATCAAAGGCCAGGTGCAGATACTCATGGGTTAAATCCAGCCGATCCt                                              >  3:576779/1‑149 (MQ=255)
                                                                                                          aGTAACAACGGAAGAAAAATCTTTCGCATAGCATTAGTCCATTAATAATTGTCGGGTCAGCGTTTCGATATAGTTCTCATCAAGCCCGGTGGGATAACCATCAAAGGCCAGGTGCAGATACTCATGGGTTAAATCCAGCCGATCCTGaa                                           <  2:30463/149‑1 (MQ=255)
                                                                                                           gTAACAACGGAAGAAAAATCTTTCGCATAGCATTAGTCCATAAATAATTGTCGGGTCAGCGTTTCGATATAGTTCTCATCAAGCCCGGTGGGATAACCATCAAAGGCCAGGTGCAGATACTCATGGGTTAAATCCAGCCGATCCTGAAg                                          <  4:56401/149‑1 (MQ=255)
                                                                                                           gTAACAACGGAAGAAAAATCTTTCGCATAGCATTAGTCCATAAATAATTGTCGGGTCAGCGTTTCGATATAGTTCTCATCAAGCCCGGTGGGATAACCATCAAAGGCCAGGTGCAGATACTCATGGGTTAAATCCAGCCGATCCTGAAg                                          <  2:558307/149‑1 (MQ=255)
                                                                                                            tAACAACGGAAGAAAAATCTTTCGCATAGCATTAGTCCATTAATAATTGTCGGGTCAGCGTTTCGATATAGTTCTCATCAAGCCCGGTGGGATAACCATCAAAGGCCAGGTGCAGATACTCATGGGTTAAATCCAGCCGATCCTGAAGc                                         >  2:62907/1‑149 (MQ=255)
                                                                                                                 acGGAAGAAAAATCTTTCGCATAGCATTAGTCCATTAATAATTGTCGGGTCAGCGTTTCGATATAGTTCTCATCAAGCCCGGTGGGATAACCATCAAAGGCCAGGTGCAGATACTCATGGGTTAAATCCAGCCGATCCTGAAGCGTaaa                                    <  1:241632/149‑1 (MQ=255)
                                                                                                                   ggAAGAAAAATCTTTCGCATAGCATTAGTCCATTAATAATTGTCGGGTCAGCGTTTCGATATAGTtc                                                                                                                    <  1:219369/67‑1 (MQ=255)
                                                                                                                   ggAAGAAAAATCTTTCGCATAGCATTAGTCCATTAATAATTGTCGGGTCAGCGTTTCGATATAGTtc                                                                                                                    >  2:219369/1‑67 (MQ=255)
                                                                                                                   ggAAGAAAAATCTTTCGCATAGCATTAGTCCATTAATAATTGTCGGGTCAGCGTTTCGATATAGTTCTCATCAAGCCCGGTGGGATAACCATCAAAGGCCAGGTGCAGATACTCATGGGTTAAATCCAGCCGATCCTGAAGCGTAaaga                                  >  4:86806/1‑149 (MQ=255)
                                                                                                                       gaaAAATCTTTCGCATAGCATTAGTCCATTAATAATTGTCGGGTCAGCGTTTCGATATAGTTCTCATCAAGCCCGGTGGGATAACCATCAAAGGCCAGGTGCAGATACTCATGGGTTAAATCCAGCCGATCCTGAAGCGTAAAGAAGtt                              >  1:191283/1‑149 (MQ=255)
                                                                                                                            aTCTTTCGCATAGCATTAGTCCATTAATAATTGTCGGGTCAGCGTTTCGATATAGTTCTCATCAAGCCCGGTGGGATAACCATCAAAGGCCAGGTGCAGATACTCATGGGTTAAATCCAGCCGATCCTGAAGCGTAAAGAAGTTACGaa                         <  1:62907/149‑1 (MQ=255)
                                                                                                                               tttCGCATAGCATTAGTCCATTAATAATTGTCGGGTCAGCGTTTCGATATAGTTCTCATCAAGCCCGGTGGGATAACCATCAAAGGCCAGGTGCAGATACTCATGGGTTAAATCCAGCCGATCCTGAAGCGTAAAGAAGTTACGAATaa                      >  2:462846/1‑149 (MQ=255)
                                                                                                                                ttCGCATAGCATTAGTCCATTAATAATTGTCGGGTCAGCGTTTCGATATAGTTCTCATCAAGCCCGGTGGGATAACCATCAAAGGCCAGGTGCAGATACTCATGGGTTAAATCCAGCCGATCCTGAAGCGTAAAGAAGTTACGAATaaa                     <  4:380976/149‑1 (MQ=255)
                                                                                                                                  cGCATAGCATTAGTCCATTAATAATTGTCGGGTCAGCGTTTCGATATAGTTCTCATCAAGCCCGGTGGGATAACCATCAAAGGCCAGGTGCAGATACTCATGGGTTAAATCCAGCCGATCCTGAAGCGTAAAGAAGTTACGAATAAACa                   <  4:6465/149‑1 (MQ=255)
                                                                                                                                  cGCATAGCATTAGTCCATTAATAATTGTCGGGTCAGCGTTTCGATATAGTTCTCATCAAGCCCGGTGGGATAACCATCAAAGGCCAGGTGCAGATACTCATGGGTTAAATCCAGCCGATCCTGAAGCGTAAAGAAGTTACGAATAAACa                   <  4:241154/149‑1 (MQ=255)
                                                                                                                                      tAGCATTAGTCCATTAATAATTGTCGGGTCAGCGTTTCGATATAGTTCTCATCAAGCCCGGTGGGATAACCATCAAAGGCCAGGTGCAGATACTCATGGGTTAAATCCAGCCGATCCTGAAGCGTAAAGAAGTTACGAATAAACAGCCg               >  4:281477/1‑149 (MQ=255)
                                                                                                                                          aTTAGTCCATTAATAATTGTCGGGTCAGCGTTTCGATATAGTTCTCATCAAGCCCGGTGGGATAACCATCAAAGGCCAGGTGCAGATACTCATGGGTTAAATCCAGCCGATCCTGAAGCGTAAAGAAGTTACGAATAAACAGCCGTTTc           <  2:165175/149‑1 (MQ=255)
                                                                                                                                          aTTAGTCCATTAATAATTGTCGGGTCAGCGTTTCGATATAGTTCTCATCAAGCCCGGTGGGATAACCATCAAAGGCCAGGTGCAGATACTCATGGGTTAAATCCAGCCGATCCTGAAGCGTAAAGAAGTTACGAATAAACAGCCGTTTc           >  1:336221/1‑149 (MQ=255)
                                                                                                                                              gTCCATTAATAATTGTCGGGTCAGCGTTTCGATATAGTTCTCATCAAGCCCGGTGGGATAACCATCAAAGGCCAGGTGCAGATACTCATGGGTTAAATCCAGCCGATCCTg                                             <  4:384067/111‑1 (MQ=255)
                                                                                                                                              gTCCATTAATAATTGTCGGGTCAGCGTTTCGATATAGTTCTCATCAAGCCCGGTGGGATAACCATCAAAGGCCAGGTGCAGATACTCATGGGTTAAATCCAGCCGATCCTg                                             >  3:384067/1‑111 (MQ=255)
                                                                                                                                               tCCATTAATAATTGTCGGGTCAGCGTTTCGATATAGTTCTCATCAAGCCCGGTGGGATAACCATCAAAGGCCAGGTGCAGATACTCATGGGTTAAATCCAGCCGATCCTGAAGCGTAAAGAAGTTACGaa                         <  1:460595/130‑1 (MQ=255)
                                                                                                                                               tCCATTAATAATTGTCGGGTCAGCGTTTCGATATAGTTCTCATCAAGCCCGGTGGGATAACCATCAAAGGCCAGGTGCAGATACTCATGGGTTAAATCCAGCCGATCCTGAAGCGTAAAGAAGTTACGaa                         >  2:460595/1‑130 (MQ=255)
                                                                                                                                               tCCATTAATAATTGTCGGGTCAGCGTTTCGATATAGTTCTCATCAAGCCCGGTGGGATAACCATCAAAGGCCAGGTGCAGATACTCATGGGTTAAATCCAGCCGATCCTGAAGCGTAAAGAAGTTACGAATAAACAGCCGTTTctgctg      >  2:73137/1‑149 (MQ=255)
                                                                                                                                               tCCATTAATAATTGTCGGGTCAGCGTTTCGATATAGTTCTCATCAAGCCCGGTGGGATAACCATCAAAGGCCAGGTGCAGATACTCATAGGTTAAATCCAGCCGATCCTGAAGCGTAAAGAAGTTACGAATAAACAGCCGTTTctgctg      >  3:405320/1‑149 (MQ=255)
                                                                                                                                                 cATTAATAATTGTCGGGTCAGCGTTTCGATATAGTTCTCATCAAGCCCGGTGGGATAACCATCAAAGGCCAGGTGCAGATACTCATGGGTTAAATCCAGCCGATCCTGAAGCGTAAAGAAGTTACGAATAAACAGCCGTTTCTGCTGcc    >  2:166572/1‑149 (MQ=255)
                                                                                                                                                   ttAATAATTGTCGGGTCAGCGTTTCGATATAGTTCTCATCAAGCCCGGTGGGATAACCATCAAAGGCCAGGTGCAGATACTCATGGGTTAAATCCAGCCGATCCTGAAGCGTAAAGAAGTTACGAATAAACAGCCGTTTCTGCTGCCGa  >  2:495794/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TGAAAAACTGGCATCTTCGCCCTCTGCCGGATGCCAGCCTGAGAGCGGTGCATCAATCTCGACTTCCTGCACACCTTCACTGTGAGCGACAGGCGAAAGCGCCACCAGTAACAACGGAAGAAAAATCTTTCGCATAGCATTAGTCCATTAATAATTGTCGGGTCAGCGTTTCGATATAGTTCTCATCAAGCCCGGTGGGATAACCATCAAAGGCCAGGTGCAGATACTCATGGGTTAAATCCAGCCGATCCTGAAGCGTAAAGAAGTTACGAATAAACAGCCGTTTCTGCTGCCGA  >  NC_000913/2328009‑2328304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑