Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,497,024 T→G 10.1% intergenic (+32/+33) ypdK → / ← alaC inner membrane protein/glutamate‑pyruvate aminotransferase; glutamic‑pyruvic transaminase (GPT); alanine transaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,497,0240TG10.1% 190.3 / 10.5 59intergenic (+32/+33)ypdK/alaCinner membrane protein/glutamate‑pyruvate aminotransferase; glutamic‑pyruvic transaminase (GPT); alanine transaminase
Reads supporting (aligned to +/- strand):  ref base T (28/25);  new base G (2/4);  total (30/29)
Fisher's exact test for biased strand distribution p-value = 4.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAAGTCATTCAGGCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCAGT  >  NC_000913/2496878‑2497171
                                                                                                                                                  |                                                                                                                                                   
gAAGTCATTCAGGCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCa                                                                                                                                                   >  2:564997/1‑149 (MQ=255)
gAAGTCATTCAGGCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCa                                                                                                                                                   <  1:69996/149‑1 (MQ=255)
  aGTCATTCAGGCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAgg                                                                                                                                                 <  3:475935/149‑1 (MQ=255)
   gTCATTCAGGCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGTCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAACACAGGAGCCATCAGGc                                                                                                                                                >  1:351714/1‑149 (MQ=255)
   gTCATTCAGGCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGc                                                                                                                                                >  2:466360/1‑149 (MQ=255)
    tCATTCAGGCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCt                                                                                                                                               >  3:450283/1‑149 (MQ=255)
     cATTCAGGCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTc                                                                                                                                              <  4:450283/149‑1 (MQ=255)
      aTTCAGGCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTcc                                                                                                                                             >  3:403764/1‑149 (MQ=255)
          aGGCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCTGATGGCTCCTGtt                                                                                                                                         <  4:208768/149‑1 (MQ=255)
           ggCGTAATAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATCAAAAACAGGAGCCATCAGGCTCCTGttt                                                                                                                                        <  3:503186/149‑1 (MQ=255)
             cGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCTGATGGCTCCTGttttt                                                                                                                                      <  4:532561/149‑3 (MQ=255)
              gTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGGCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCTGATGGCTCCTGtt                                                                                                                                         <  3:387999/145‑1 (MQ=255)
              gTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCTGATGGCTCCTGtt                                                                                                                                         >  4:387999/1‑145 (MQ=255)
              gTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCa                                                                                                                                     >  3:139361/1‑149 (MQ=255)
                  cAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAGACAGGAGCCATCAGGCTCCTGTTTGCATTTc                                                                                                                                 <  3:387342/149‑1 (MQ=255)
                   aGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCt                                                                                                                                <  1:413145/149‑1 (MQ=255)
                    gTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTg                                                                                                                               >  4:495492/1‑149 (MQ=255)
                     tAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTga                                                                                                                              <  3:5928/149‑1 (MQ=255)
                      aaTTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTgac                                                                                                                             >  1:35576/1‑149 (MQ=255)
                               gagagaTGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGtt                                                                                                                    >  3:497322/1‑149 (MQ=255)
                                           gTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTGTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGttt                                                                                                        >  1:383765/1‑149 (MQ=255)
                                               aaTATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGt                                                                                                    <  2:175097/149‑1 (MQ=255)
                                                  atTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGaa                                                                                                 >  1:243369/1‑149 (MQ=255)
                                                    ttCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAata                                                                                               <  3:527106/149‑1 (MQ=255)
                                                              gCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAGCTGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAata                                                                                               <  3:485413/139‑1 (MQ=255)
                                                              gCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCTGATGGCTCCTGttt                                                                                                                                        >  4:472063/1‑98 (MQ=39)
                                                              gCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCTGATGGCTCCTGttt                                                                                                                                        <  3:472063/98‑1 (MQ=39)
                                                              gCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAata                                                                                               >  4:485413/1‑139 (MQ=255)
                                                              gCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTtgctgc                                                                                     >  4:48126/1‑149 (MQ=255)
                                                                aTTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGctgctg                                                                                   <  1:466360/149‑1 (MQ=255)
                                                                    cTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCgg                                                                               <  3:463201/149‑1 (MQ=255)
                                                                     ttttATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCggg                                                                              >  3:455132/1‑149 (MQ=255)
                                                                       ttATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGgta                                                                            <  1:235023/149‑1 (MQ=255)
                                                                          tGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGgtagta                                                                         >  2:230579/1‑149 (MQ=255)
                                                                              cATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAAcc                                                                     <  1:564997/149‑1 (MQ=255)
                                                                               aTCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCg                                                                    >  3:69854/1‑149 (MQ=255)
                                                                                        gCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGgta                                                                            <  3:291798/132‑1 (MQ=255)
                                                                                        gCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGgta                                                                            >  4:291798/1‑132 (MQ=255)
                                                                                            gTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACa                                                       >  3:573256/1‑149 (MQ=255)
                                                                                                 tttGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCt                                                  <  4:403764/149‑1 (MQ=255)
                                                                                                      ccTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGt                                                                                                    <  1:135543/94‑1 (MQ=255)
                                                                                                      ccTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGt                                                                                                    >  2:135543/1‑94 (MQ=255)
                                                                                                      ccTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAAt                                             >  3:454189/1‑149 (MQ=255)
                                                                                                        tgatgaTCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCTCTTTAATcc                                           >  1:183493/1‑149 (MQ=255)
                                                                                                         gatgatCTAAAGGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCATTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATccc                                          >  3:386450/1‑149 (MQ=255)
                                                                                                           tgatCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATGGCTTTAATCCctc                                        >  4:428375/1‑149 (MQ=255)
                                                                                                              tCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGa                                     <  2:183493/149‑1 (MQ=255)
                                                                                                                tAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATg                                   <  3:48126/149‑1 (MQ=255)
                                                                                                                tAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATg                                   <  2:243369/149‑1 (MQ=255)
                                                                                                                    aGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCt                               >  4:245979/1‑149 (MQ=255)
                                                                                                                            gtgCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATa                       <  4:497322/149‑1 (MQ=255)
                                                                                                                              gCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACg                     <  4:455132/149‑1 (MQ=255)
                                                                                                                                  tGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTcg                 >  4:535676/1‑149 (MQ=255)
                                                                                                                                   gAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTcgc                >  4:321598/1‑149 (MQ=255)
                                                                                                                                   gAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTcgc                >  1:78053/1‑149 (MQ=255)
                                                                                                                                      aaaCAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGt             >  3:16444/1‑149 (MQ=255)
                                                                                                                                       aaCAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGtt            <  1:333480/149‑1 (MQ=255)
                                                                                                                                               ccATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCa    <  2:351714/149‑1 (MQ=255)
                                                                                                                                                 aTCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCAGt  <  3:495492/149‑1 (MQ=255)
                                                                                                                                                  tCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTaa                                              >  2:179875/1‑104 (MQ=255)
                                                                                                                                                  tCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTaa                                              <  1:179875/104‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GAAGTCATTCAGGCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCAGT  >  NC_000913/2496878‑2497171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: