Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,656,392 T→C 7.2% intergenic (‑34/+144) pepB ← / ← iscX aminopeptidase B/Fe(2+) donor and activity modulator for cysteine desulfurase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,656,3920TC 7.2% 217.9 / 6.1 69intergenic (‑34/+144)pepB/iscXaminopeptidase B/Fe(2+) donor and activity modulator for cysteine desulfurase
Reads supporting (aligned to +/- strand):  ref base T (37/27);  new base C (2/3);  total (39/30)
Fisher's exact test for biased strand distribution p-value = 6.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.20e-01

CCCGTTCAAATGCAGGGTAATGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTGCGGCGTGAAGCATGACGCGGAAA  >  NC_000913/2656248‑2656535
                                                                                                                                                |                                                                                                                                               
cccGTTCAAATGCAGGGTAATGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGc                                                                                                                                             >  4:564223/1‑149 (MQ=255)
 ccGTTCAAATGCAGGGTAATGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGtt                                                                                                                                                >  3:314563/1‑145 (MQ=255)
 ccGTTCAAATGCAGGGTAATGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGtt                                                                                                                                                <  4:314563/145‑1 (MQ=255)
   gTTCAAATGCAGGGTAATGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGc                                                                                                                                          >  1:119141/1‑149 (MQ=255)
     tCAAATGCAGGGTAATGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCTGCCAACTGGCGGccc                                                                                                                                        <  1:451122/149‑1 (MQ=255)
     tCAAATGCAGGGTAATGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCTGCCAACTGGCGGccc                                                                                                                                        <  3:440068/149‑1 (MQ=255)
           gCAGGGTAATGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAg                                                                                                                                     >  2:382019/1‑146 (MQ=255)
           gCAGGGTAATGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAg                                                                                                                                     <  1:382019/146‑1 (MQ=255)
            cAGGGTAATGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGAtt                                                                                                                                 <  3:140904/149‑1 (MQ=255)
             aGGGTAATGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTTATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGAttt                                                                                                                                <  4:403903/149‑1 (MQ=255)
                       cGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTaaa                                                                                                                      <  1:128872/149‑1 (MQ=255)
                        gTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAAt                                                                                                                     >  2:591650/1‑149 (MQ=255)
                        gTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAAt                                                                                                                     >  2:82055/1‑149 (MQ=255)
                           antattAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGAt                                                                                                                  >  3:113192/1‑149 (MQ=255)
                            ttattaATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGAtt                                                                                                                 <  1:136009/149‑1 (MQ=255)
                                  aTGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGtt                                                                                                           <  2:71013/149‑1 (MQ=255)
                                     cTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCa                                                                                                        <  1:229490/149‑1 (MQ=255)
                                     cTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGAGTTGCa                                                                                                        <  2:422647/149‑1 (MQ=255)
                                           gTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAgg                                                                                                  <  2:299373/149‑1 (MQ=255)
                                            ttGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAggc                                                                                                 >  4:6455/1‑149 (MQ=255)
                                            ttGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAggc                                                                                                 >  2:104682/1‑149 (MQ=255)
                                            ttGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAggc                                                                                                 <  4:26154/149‑1 (MQ=255)
                                             tGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAggcg                                                                                                <  1:553465/149‑1 (MQ=255)
                                               cTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAggcggc                                                                                              >  3:339435/1‑149 (MQ=255)
                                                tttttCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCa                                                                                             >  3:379543/1‑149 (MQ=255)
                                                tttttCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCa                                                                                             >  4:425940/1‑149 (MQ=255)
                                                 ttttCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCaa                                                                                            <  3:126172/149‑1 (MQ=255)
                                                 ttttCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCaa                                                                                            <  4:32770/149‑1 (MQ=255)
                                                       ccccAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAagtgagt                                                                                      >  2:99188/1‑149 (MQ=255)
                                                        cccAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAagtgagtg                                                                                     <  1:104682/149‑1 (MQ=255)
                                                           aGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAg                                                                                  <  2:455919/149‑1 (MQ=255)
                                                                gcgTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCa                                                                             <  4:293877/149‑1 (MQ=255)
                                                                 cgTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAg                                                                            <  1:275567/149‑1 (MQ=255)
                                                                   tCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGa                                                                          <  2:119141/149‑1 (MQ=255)
                                                                      gCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGGGCa                                                                       >  1:591083/1‑149 (MQ=255)
                                                                        aGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATa                                                                     >  3:34599/1‑149 (MQ=255)
                                                                          gTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGAt                                                                                                                  >  4:258029/1‑102 (MQ=255)
                                                                          gTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGAt                                                                                                                  <  3:258029/102‑1 (MQ=255)
                                                                             gggTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATaa                                                                <  1:266995/149‑1 (MQ=255)
                                                                               gTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACt                                                              >  1:445952/1‑149 (MQ=255)
                                                                                       ggTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGccc                                                                                                                                        >  4:133476/1‑67 (MQ=255)
                                                                                       ggTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGccc                                                                                                                                        <  3:133476/67‑1 (MQ=255)
                                                                                       ggTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGAAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACt                                                      >  2:211513/1‑149 (MQ=255)
                                                                                        gTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTg                                                     >  4:558628/1‑149 (MQ=255)
                                                                                         tAATCTTCATCGCTTCTGTCATCTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTgg                                                    >  2:287591/1‑149 (MQ=255)
                                                                                          aaTCTTCATCGCTTCTGTCATTCTAGTTATCCTTCTTTGTAAACGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGAGACTggg                                                   >  2:219223/1‑149 (MQ=255)
                                                                                          aaTCTTCATCGCTTCTGTCATCTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTggg                                                   <  1:287591/149‑1 (MQ=255)
                                                                                                  tCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGa                                           >  2:57404/1‑149 (MQ=255)
                                                                                                  tCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGa                                           <  1:57404/149‑1 (MQ=255)
                                                                                                          gTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGcc                                   >  2:309052/1‑149 (MQ=255)
                                                                                                          gTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACAATGTGACTGGGGTGAACGAGCGCAGcc                                   <  3:391730/149‑1 (MQ=255)
                                                                                                              ttttAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACg                               >  2:302023/1‑149 (MQ=255)
                                                                                                              ttttAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACg                               >  1:503014/1‑149 (MQ=255)
                                                                                                                  aGTTAACCTGCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTAAGTGACTGGGGTGAACGAGCGCAGCCAACGCATc                           <  1:99188/149‑1 (MQ=255)
                                                                                                                        ccTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTgcggc                     >  3:313553/1‑149 (MQ=255)
                                                                                                                          ttcttTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTGCGGCGt                   >  2:571488/1‑149 (MQ=255)
                                                                                                                          ttcttTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTGCGGCGt                   >  3:509280/1‑149 (MQ=255)
                                                                                                                          ttcttTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTGCGGCGt                   >  1:215872/1‑149 (MQ=255)
                                                                                                                          ttcttTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTGCGGCGt                   >  1:169001/1‑149 (MQ=255)
                                                                                                                             tttGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTGCGGCGTGaa                >  2:72/1‑149 (MQ=255)
                                                                                                                              ttGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTGCGGCGTGAAg               >  2:349510/1‑149 (MQ=255)
                                                                                                                                gTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTGCGGCGTGAAGCa             >  1:496994/1‑149 (MQ=255)
                                                                                                                                  aaaaGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGa                                           <  2:112236/117‑1 (MQ=255)
                                                                                                                                  aaaaGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGa                                           >  1:112236/1‑117 (MQ=255)
                                                                                                                                     ggggCTGCCAACTGGCGGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTGCGGCGTGAAGCATGAcg        >  2:45203/2‑149 (MQ=255)
                                                                                                                                      gggCTGCCAACTGGCGGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTGCGGCGTGaa                <  1:355809/140‑1 (MQ=37)
                                                                                                                                      gggCTGCCAACTGGCGGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTGCGGCGTGaa                >  2:355809/1‑140 (MQ=255)
                                                                                                                                      gggCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTGCGGCGTGAAGCATGAcgc       >  4:491486/1‑149 (MQ=255)
                                                                                                                                           gccAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTGCGGCGTGAAGCATGACGCGGaaa  >  3:575217/1‑149 (MQ=255)
                                                                                                                                               gTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGaa                                              >  4:256535/1‑101 (MQ=255)
                                                                                                                                               gTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGaa                                              <  3:256535/101‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                               
CCCGTTCAAATGCAGGGTAATGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGGCCGCCAGTTGGCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTGCGGCGTGAAGCATGACGCGGAAA  >  NC_000913/2656248‑2656535

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: