Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,892,552 C→T 6.4% T113T (ACC→ACT) ‡ queD → 6‑pyruvoyl tetrahydrobiopterin synthase (PTPS)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,892,5520CT 6.4% 200.7 / 3.5 62T113T (ACC→ACT) ‡queD6‑pyruvoyl tetrahydrobiopterin synthase (PTPS)
Reads supporting (aligned to +/- strand):  ref base C (27/31);  new base T (2/2);  total (29/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.35e-01

CCTACGAGCGCCTCGATCACCATTATCTCAATGATATTCCAGGTCTGGAAAACCCAACCAGCGAGGTTTTAGCAAAATGGATTTGGGATCAGGTTAAACCCGTTGTGCCGCTGTTAAGTGCGGTGATGGTAAAAGAAACCTGCACCGCAGGTTGTATCTATCGCGGCGAATGATAAGAGTGTGTCGGCGGTCAATTTCCCTTAAGTAACGCTATGTTAGGGTGTTGTGTTCTGGATATCTGGGGCATGACATGGAAGACGACTGCGACATTATTATTATTGGTGCCGGTATTGC  >  NC_000913/2892407‑2892700
                                                                                                                                                 |                                                                                                                                                    
ccTACGAGCGCCTCGATCACCATTATCTCAATGATATTCCAGGTCTGGAAAACCCAACCAGCGAGGTTTTAGCAAAATGGATTTGGGATCAGGTTAAACCCGTTGTGCCGCTGTTAAGTGCGGTGATGGTAAAAGAAACCTGCACCGCa                                                                                                                                                   >  2:149563/1‑149 (MQ=255)
 cTACGAGCGCCTCGATCACCATTATCTCAATGATATTCCAGGTCTGGAAAACCCAACCAGCGAGGTTTTAGCAAAATGGATTTGGGATCAGGTTAAACCCGTTGTGCCGCTGTTAAGTGCGGTGATGGTAAAAGAAACCTGCACCGCAg                                                                                                                                                  >  2:175115/1‑149 (MQ=255)
  tACGAGCGCCTCGATCACCATTATCTCAATGATATTCCAGGTCTGGAAAACCCAACCAGCGAGGTTTTAGCAAAATGGATTTGGGATCAGGTTAAACCCGTTGTGCCGCTGTTAAGTGCGGTGATGGTAAAAGAAACCTGCACCGCAgg                                                                                                                                                 >  3:434023/1‑149 (MQ=255)
      aGCGCCTCGATCACCATTATCTCAATGATATTCCAGGTCTGGAAAACCCAACCAGCGAGGTTTTAGCAAAATGGATTTGGGATCAGGTTAAACCCGTTGTGCCGCTGTTAAGTGCGGTGATGGTAAAAGAAACCTGCACCGCAGGTTGt                                                                                                                                             <  1:149563/149‑1 (MQ=255)
       gcgcCTCGATCACCATTATCTCAATGATATTCCAGGTCTGGAAAACCCAACCAGCGAGGTTTTAGCAAAATGGATTTGGGATCAGGTTAAACCCGTTGTGCCGCTGTTAAGTGCGGTGATGGTAAAAGAAACCTGCACCGCAGGTTGta                                                                                                                                            <  3:566155/149‑1 (MQ=255)
        cgcCTCGATCACCATTATCTCAATGATATTCCAGGTCTGGAAAACCCAACCAGCGAGGTTTTAGCAAAATGGATTTGGGATCAGGTTAAACCCGTTGTGCCGCTGTTAAGTGCGGTGATGGTAAAAGAAACCTGCACCGCAGGTTGtat                                                                                                                                           <  3:516575/149‑1 (MQ=255)
           cTCGATCACCATTATCTCAATGATATTCCAGGTCTGGAAAACCCAACCAGCGAGGTTTTAGCAAAATGGATTTGGGATCAGGTTAAACCCGTTGTGCCGCTGTTAAGTGCGGTGATGGTAAAAGAAACCTGCACCGCAGGtt                                                                                                                                               <  1:121327/142‑1 (MQ=255)
           cTCGATCACCATTATCTCAATGATATTCCAGGTCTGGAAAACCCAACCAGCGAGGTTTTAGCAAAATGGATTTGGGATCAGGTTAAACCCGTTGTGCCGCTGTTAAGTGCGGTGATGGTAAAAGAAACCTGCACCGCAGGtt                                                                                                                                               >  2:121327/1‑142 (MQ=255)
           cTCGATCACCATTATCTCAATGATATTCCAGGTCTGGAAAACCCAACCAGCGAGGTTTTAGCAAAATGGATTTGGGATCAGGTTAAACCCGTTGTGCCGCTGTTAAGTGCGGTGATGGTAAAAGAAACCTGCACCGCAGGTTGtatcta                                                                                                                                        >  2:98795/1‑149 (MQ=255)
            tCGATCACCATTATCTCAATGATATTCCAGGTCTGGAAAACCCAACCAGCGAGGTTTTAGCAAAATGGATTTGGGATCAGGTTAAACCCGTTGTGCCGCTGTTAAGTGCGGTGATGGTAAAAGAAACCTGCACCGCAGGTTGtatctat                                                                                                                                       >  4:309359/1‑149 (MQ=255)
                     aTTATCTCAATGATATTCCAGGTCTGGAAAACCCAACCAGCGAGGTTTTAGCAAAATGGATTTGGGATCAGGTTAAACCCGTTGTGCCGCTGTTAAGTGCGGTGATGGTAAAAGAAACCTGCACCGCAGGTTGTATCTATCGCGGCGaa                                                                                                                              >  1:478233/1‑149 (MQ=255)
                      ttATCTCAATGATATTCCAGGTCTGGAAAACCCAACCAGCGAGGTTTTAGCAAAATGGATTTGGGATCAGGTTAAACCCGTTGTGCCGCTGTTAAGTGCGGTGATGGTAAAAGAAACCTGCACCGCAGGTTGTATCTATCGCGGCGAAt                                                                                                                             <  3:272618/149‑1 (MQ=255)
                           tcAATGATATTCCAGGTCTGGAAAACCCAACCAGCGAGGTTTTAGCAAAATGGATTTGGGATCAGGTTAAACCCGTTGTGCCGCTGTTAAGTGCGGTGATGGTAAAAGAAACCTGCGGTGCAGGtttcttt                                                                                                                                          <  1:69993/131‑6 (MQ=255)
                           tcAATGATATTCCAGGTCTGGAAAACCCAACCAGCGAGGTTTTAGCAAAATGGATTTGGGATCAGGTTAAACCCGTTGTGCCGCTGTTAAGTGCGGTGATGGTAAAAGAAACCTGCGGTGCAGGtttcttt                                                                                                                                          >  2:69993/1‑126 (MQ=255)
                           tcAATGATATTCCAGGTCTGGAAAACCCAACCAGCGAGGTTTTAGCAAAATGGATTTGGGATCAGGTTAAACCCGTTGTGCCGCTGTTAAGTGCGGTGATGGTAAAAGAAACCTGCACCGCAGGTTGTATCTATCGCGGCGAATGATaa                                                                                                                        <  4:62014/149‑1 (MQ=255)
                           tcAATGATATTCCAGGTCTGGAAAACCCAACCAGCGAGGTTTTAGCAAAATGGATTTGGGATCAGGTTAAACCCGTTGTGCCGCTGTTAAGTGCGGTGATGGTAAAAGAAACCTGCACCGCAGGTTGTATCTATCGCGGCGAATGATaa                                                                                                                        >  2:121116/1‑149 (MQ=255)
                             aaTGATATTCCAGGTCTGGAAAACCCAACCAGCGAGGTTTTAGCAAAATGGATTTGGGATCAGGTTAAACCCGTTGTGCCGCTGTTAAGTGCGGTGATGGTAAAAGAAACCTGCACCGCAGGTTGTATCTATCGCGGCGAATGATAaga                                                                                                                      >  4:205502/1‑149 (MQ=255)
                               tGATATTCCAGGTCTGGAAAACCCAACCAGCGAGGTTTTAGCAAAATGGATTTGGGATCAGGTTAAACCCGTTGTGCCGCTGTTAAGTGCGGTGATGGTAAAAGAAACCTGCACCGCAGGTTGTATCTATCGCGGCGAATGATAAGAgt                                                                                                                    >  2:398264/1‑149 (MQ=255)
                                  tatTCCAGGTCTGGAAAACCCAACCAGCGAGGTTTTAGCAAAATGGATTTGGGATCAGGTTAAACCCGTTGTGCCGCTGTTAAGTGTGGTGATGGTAAAAGAAACCTGCACCGCAGGTTGTATCTATCGCGGCGAATGATAAGAgtgtg                                                                                                                 <  2:160388/149‑1 (MQ=255)
                                         ggTCTGGAAAACCCAACCAGCGAGGTTTTAGCAAAATGGATTTGGGATCAGGTTAAACCCGTTGTGCCGCTGTTAAGTGCGGTGATGGTAAAAGAAACCTGCACCGCAGGTTGTATCTATCGCGGCGAATGATAAGAGTGTGTcggcgg                                                                                                          >  4:460165/1‑149 (MQ=255)
                                               gAAAACCCAACCAGCGAGGTTTTAGCAAAATGGATTTGGGATCAGGTTAAACCCGTTGTGCCGCTGTTAAGTGCGGTGATGGTAAAAGAAACCTGCACCGCAGGTTGTATCTATCGCGGCGAATGATAAGAgtgt                                                                                                                  <  2:114512/135‑1 (MQ=255)
                                               gAAAACCCAACCAGCGAGGTTTTAGCAAAATGGATTTGGGATCAGGTTAAACCCGTTGTGCCGCTGTTAAGTGCGGTGATGGTAAAAGAAACCTGCACCGCAGGTTGTATCTATCGCGGCGAATGATAAGAgtgt                                                                                                                  >  1:114512/1‑135 (MQ=255)
                                               gAAAACCCAACCAGCGAGGTTTTAGCAAAATGGATTTGGGATCAGGTTAAACCCGTTGTGCCGCTGTTAAGTGCGGTGATGGTAAAAGAAACCTGCACCGCAGGTTGTATCTATCGCGGCGAATGATAAGAGTGTGTCGGCGGTCAAtt                                                                                                    >  4:165174/1‑149 (MQ=255)
                                               gAAAACCCAACCAGCGAGGTTTTAGCAAAATGGATTTGGGATCAGGTTAAACCCGTTGTGCCGCTGTTAAGTGCGGTGATGGTAAAAGAAACCTGCACCGCAGGTTGTATCTATCGCGGCGAATGATAAGAGTGTGTCGGCGGTCAAtt                                                                                                    >  4:509595/1‑149 (MQ=255)
                                                 aaaCCCAACCAGCGAGGTTTTAGCAAAATGGATTTGGGATCAGGTTAAACCCGTTGTGCCGCTGTTAAGTGCGGTGATGGTAAAAGAAACCTGCACCGCAGGTTGta                                                                                                                                            >  2:8687/1‑107 (MQ=255)
                                                 aaaCCCAACCAGCGAGGTTTTAGCAAAATGGATTTGGGATCAGGTTAAACCCGTTGTGCCGCTGTTAAGTGCGGTGATGGTAAAAGAAACCTGCACCGCAGGTTGta                                                                                                                                            <  1:8687/107‑1 (MQ=255)
                                                  aaCCCAACCAGCGAGGTTTTAGCAAAATGGATTTGGGATCAGGTTAAACCCGTTGTGCCGCTGTTAAGTGCGGTGATGGTAAAAGAAACCTGCACCGCAGGTTGTATCTATCGCGGCGAATGATAAGAGTGTGTCGGCGGTCAATTTcc                                                                                                 <  2:478233/149‑1 (MQ=255)
                                                  aaCCCAACCAGCGAGGTTTTAGCAAAATGGATTTGGGATCAGGTTAAACCCGTTGTGCCGCTGTTAAGTGCGGTGATGGTAAAAGAAACCTGCACCGCAGGTTGTATCTATCGCGGCGAATGATAAGAGTGTGTCGGCGGTCAATTTcc                                                                                                 <  2:27090/149‑1 (MQ=255)
                                                    cccAACCAGCGAGGTTTTAGCAAAATGGATTTGGGATCAGGTTAAACCCGTTGTGCCGCTGTTAAGTGCGGTGATGGTAAAAGAAACCTGCACCGCAGGTTGTATCTATCGCGGCGAATGATAAGAGTGTGTCGGCGGTCAATTTCCCt                                                                                               >  4:578451/1‑149 (MQ=255)
                                                    cccAACCAGCGAGGTTTTAGCAAAATGGATTTGGGATCAGGTTAAACCCGTTGTGCCGCTGTTAAGTGCGGTGATGGTAAAAGAAACCTGCACCGCAGGTTGTATCTATCGCGGCGAATGATAAGAGTGTGTCGGCGGTCAATTTCCCt                                                                                               <  4:465101/149‑1 (MQ=255)
                                                     ccAACCAGCGAGGTTTTAGCAAAATGGATTTGGGATCAGGTTAAACCCGTTGTGCCGCTGTTAAGTGCGGTGATGGTAAAAGAAACCTGCGGTGCAGGtttc                                                                                                                                             >  2:122516/1‑100 (MQ=255)
                                                     ccAACCAGCGAGGTTTTAGCAAAATGGATTTGGGATCAGGTTAAACCCGTTGTGCCGCTGTTAAGTGCGGTGATGGTAAAAGAAACCTGCGGTGCAGGtttc                                                                                                                                             <  1:122516/102‑3 (MQ=255)
                                                     ccAACCAGCGAGGTTTTAGCAAAATGGATTTGGGATCAGGTTAAACCCGTTGTGCCGCTGTTAAGTGCGGTGATGGTAAAAGAAACCTGCACCGCAGGTTGTATCTATCGCGGCGAATGATAAGAGTGTGTCGGCGGTCAATTTCCCtt                                                                                              <  1:435490/149‑1 (MQ=255)
                                                     ccAACCAGCGAGGTTTTAGCAAAATGGATTTGGGATCAGGTTAAACCCGTTGTGCCGCTGTTAAGTGCGGTGATGGTAAAAGAAACCTGCACCGCAGGTTGTATCTATCGCGGCGAATGATAAGAGTGTGTCGGCGGTCAATTTCCCtt                                                                                              >  1:328626/1‑149 (MQ=255)
                                                     ccAACCAGCGAGGTTTTAGCAAAATGGATTTGGGATCAGGTTAAACCCGTTGTGCCGCTGTTAAGTGCGGTGATGGTAAAAGAAACCTGCACCGCAGGTTGTATCTATCGCGGCGAATGATAAGAGTGTGTCGGCGGTCAATTTCCCtt                                                                                              <  3:509595/149‑1 (MQ=255)
                                                     ccAACCAGCGAGGTTTTAGCAAAATGGATTTGGGATCAGGTTAAACCCGTTGTGCCGCTGTTAAGTGCGGTGATGGTAAAAGAAACCTGCACCGCAGGTTGTATCTATCGCGGCGAATGATAAGAGTGTGTCGGCGGTCAATTTCCCtt                                                                                              >  3:39191/1‑149 (MQ=255)
                                                       aaCCAGCGAGGTTTTAGCAAAATGGATTTGGGATCAGGTTAAACCCGTTGTGCCGCTGTTAAGTGCGGTGATGGTAAAAGAAACCTGCACCGCAGGTTGTATCTATCGCGGCGAATGATAAGAGTGTGTCGGCGGTCAATTTCCCTTaa                                                                                            >  1:15813/1‑149 (MQ=255)
                                                                       gCAAAATGGATTTGGGATCAGGTTAAACCCGTTGTGCCGCTGTTAAGTGCGGTGATGGTAAAAGAAACCTGCACCGCAGGTTGTATCTATCGCGGCGAATGATAAGAGTGTGTCGGCGGTCAATTTCCCTTAAGTAACGCTATGTTAgg                                                                            <  2:127073/149‑1 (MQ=255)
                                                                       gCAAAATGGATTTGGGATCAGGTTAAACCCGTTGTGCCGCTGTTAAGTGCGGTGATGGTAAAAGAAACCTGCACCGCAGGTTGTATCTATCGCGGCGAATGATAAGAGTGTGTCGGCGGTCAATTTCCCTTAAGTAACGCGATGTTAgg                                                                            <  3:539741/149‑1 (MQ=255)
                                                                            aTGGATTTGGGATCAGGTTAAACCCGTTGTGCCGCTGTTAAGTGCGGTGATGGTAAAAGAAACCTGCACCGCAGGTTGTATCTATCGCGGCGAATGATAAGAGTGTGTCGGCGGTCAATTTCCCTTAAGTAACGCTATGTTAGGgtgtt                                                                       <  1:232359/149‑1 (MQ=255)
                                                                                  ttGGGATCAGGTTAAACCCGTTGTGCCGCTGTTAAGTGCGGTGATGGTAAAAGAAACCTGCACCGCAGGTTGTATCTATCGCGGCGAATGATAAGAGTGTGTCGGCGGTCAATTTCCCTTAAGTAACGCTATGTTAGGGTGTTGTGTTc                                                                 <  4:30075/149‑1 (MQ=255)
                                                                                    gggATCAGGTTAAACCCGTTGTGCCGCTGTTAAGTGCGGTGATGGTAAAAGAAACCTGCACCGCAGGTTGTATCTATCGCGGCGAATGATAAGAGTGTGTCGGCGGTCAATTTCCCTTAAGTAACGCTATGTTAGGGTGTTGTGTTCTg                                                               <  3:205502/149‑1 (MQ=255)
                                                                                    gggATCAGGTTAAACCCGTTGTGCCGCTGTTAAGTGCGGTGATGGTAAAAGAAACCTGCACCGCAGGTTGTATCTATCGCGGCGAATGATAAGAGTGTGTCGGCGGTCAATTTCCCTTAAGTAACGCTATGTTAGGGTGTTGTGTTCTg                                                               <  4:434023/149‑1 (MQ=255)
                                                                                      gATCAGGTTAAACCCGTTGTGCCGCTGTTAAGTGCGGTGATGGTAAAAGAAACCTGCACCGCAGGTTGTATCTATCGCGGCGAATGATAAGAGTGTGTCGGCGGTCAATTTCCCTTAAGTAACGCTATGTTAGGGTGTTGTGTTCTGGa                                                             >  4:222141/1‑149 (MQ=255)
                                                                                       aTCAGGTTAAACCCGTTGTGCCGCTGTTAAGTGCGGTGATGGTAAAAGAAACCTGCACCGCAGGTTGTATCTATCGCGGCGAATGATAAGAGTGTGTCGGCGGTCAATTTCCCTTAAGTAACGCTATGTTAgg                                                                            >  3:423470/1‑133 (MQ=255)
                                                                                       aTCAGGTTAAACCCGTTGTGCCGCTGTTAAGTGCGGTGATGGTAAAAGAAACCTGCACCGCAGGTTGTATCTATCGCGGCGAATGATAAGAGTGTGTCGGCGGTCAATTTCCCTTAAGTAACGCTATGTTAgg                                                                            <  4:423470/133‑1 (MQ=255)
                                                                                        tCAGGTTAAACCCGTTGTGCCGCTGTTAAGTGCGGTGATGGTAAAAGAAACCTGCACCGCAGGTTGTATCTATCGCGGCGAATGATAAGAGTGTGTCGGCGGTCAATTTCCCTTAAGTAACGCTATGTTAGGGTGTTGTGTTCTGGata                                                           >  3:149155/1‑149 (MQ=255)
                                                                                             ttAAACCCGTTGTGCCGCTGTTAAGTGCGGTGATGGTAAAAGAAACCTGCACCGCAGGTTGTATCTATCGCGGCGAATGATAAGAGTGTGTCGGCGGTCAATTTCCCTTAAGTAACGCTATGTTAGGGTGTTGTGTTCTGGATATCTgg                                                      <  2:554252/149‑1 (MQ=255)
                                                                                              tAAACCCGTTGTGCCGCTGTTAAGTGCGGTGATGGTAAAAGAAACCTGCACCGCAGGTTGTATCTATCGCGGCGAATGATAAGAGTGTGTCGGCGGTCAATTTCCCTTAAGTAACGCTATGTTAGGGTGTTGTGTTCTGGATATCTggg                                                     <  2:15813/149‑1 (MQ=255)
                                                                                                  cccGTTGTGCCGCTGTTAAGTGCGGTGATGGTAAAAGAAACCTGCACCGCAGGTTGTATCTATCGCGGCGAATGATAAGAGTGTGTCGGCGGTCAATTTCCCTTAAGTAACGCTATGTTAGGGTGTTGTGTTCTGGATATCTGGGGCAt                                                 >  3:297115/1‑149 (MQ=255)
                                                                                                  cccGTTGTGCCGCTGTTAAGTGCGGTGATGGTAAAAGAAACCTGCACCGCAGGTTGTATCTATCGCGGCGAATGATAAGAGTGTGTCGGCGGTCAATTTCCCTTAAGTAACGCTATGTTAGGGTGTTGTGTTCTGGATATCTGGGGCAt                                                 >  4:358284/1‑149 (MQ=255)
                                                                                                     gTTGTGCCGCTGTTAAGTGCGGTGATGGTAAAAGAAACCTGCACCGGAGGTTGTATCTATCGCGGCGAATGATAAGAGTGTGTCGGCGGTCAATTTCCCTTAAGTAACGCTATGTTAGGGTGTTGTGTTCTGGATATCTGGGGCATGAc                                              <  1:175115/149‑1 (MQ=255)
                                                                                                        gtgCCGCTGTTAAGTGCGGTGATGGTAAAAGAAACCTGCACCGCAGGTTGTATCTATCGCGGCGAATGATAAGAGTGTGTCGGCGGTCAATTTCCCTTAAGTAACGCTATGTTAGGGTGTTGTGTTCTGGATATCTGGGGCATGACATg                                           <  1:98795/149‑1 (MQ=255)
                                                                                                         tgCCGCTGTTAAGTGCGGTGATGGTAAAAGAAACCTGCACCGCAGGTTGTATCTATCGCGGCGAATGATAAGAGTGTGTCGGCGGTCAATTTCCCTTAAGTAACGCTATGTTAGGGTGTTGTGTACTGGATATCTGGGGCATGACATgg                                          <  2:144506/149‑1 (MQ=255)
                                                                                                           ccGCTGTTAAGTGCGGTGATGGTAAAAGAAACCTGCACCGCAGGTTGTATCTATCGCGGCGAATGATAAGAGTGTGTCGGCGGTCAATTTCCCTTAAGTAACGCTATGTTAGGGTGTTGTGTTCTGGATATCTGGGGCATGACATGGaa                                        <  3:165174/149‑1 (MQ=255)
                                                                                                           ccGCTGTTAAGTGCGGTGATGGTAAAAGAAACCTGCACCGCAGGTTGTATCTATCGCGGCGAATGATAAGAGTGTGTCGGCGGTCAATTTCCCTTAAGTAACGCTATGTTAGGGTGTTGTGTTCTGGATATCTGGGGCATGACATGGaa                                        <  2:235905/149‑1 (MQ=255)
                                                                                                                gTTAAGTGCGGTGATGGTAAAAGAAACCTGCACCGCAGGTTGTATCTATCGCGGCGAATGATAAGAGTGTGTCGGCGGTCAATTTCCCTTAAGTAACGCTATGTTAGGGTGTTGTGTTCTGGATATCTGGGGCATGACATGGAAgacga                                   <  3:358284/149‑1 (MQ=255)
                                                                                                                 ttAAGTGCGGTGATGGTAAAAGAAACCTGCACCGCAGGTTGTATCTATCGCGGCGAATGATAAGAGTGTGTCGGCGGTCAATTTCCCTTAAGTAACGCTATGTTAGGGTGTTGTGTTCTGGATATCTgggg                                                    >  2:75444/1‑131 (MQ=255)
                                                                                                                 ttAAGTGCGGTGATGGTAAAAGAAACCTGCACCGCAGGTTGTATCTATCGCGGCGAATGATAAGAGTGTGTCGGCGGTCAATTTCCCTTAAGTAACGCTATGTTAGGGTGTTGTGTTCTGGATATCTgggg                                                    <  1:75444/131‑1 (MQ=255)
                                                                                                                                 tAAAAGAAACCTGCACCGCAGGTTGTATCTATCGCGGCGAATGATAAGAGTGTGTCGGCGGTCAATTTCCCTTAAGTAACGCTATGTTAGGGTGTTGTGTTCTGGATATCTGGGGCATGACATGGAAGACGACTGCGACattattatta                  <  4:277891/149‑1 (MQ=255)
                                                                                                                                 tAAAAGAAACCTGCACCGCAGGTTGTATCTATCGCGGCGAATGATAAGAGTGTGTCGGCGGTCAATTTCCCTTAAGTAACGCTATGTTAGGGTGTTGTGTTCTGGATATCTGGGGCATGACATGGAAGACGACTGCGACattattatta                  >  1:83645/1‑149 (MQ=255)
                                                                                                                                      gAAACCTGCACCGCAGGTTGTATCTATCGCGGCGAATGATAAGAGTGTGTCGGCGGTCAATTTCCCTTAAGTAACGCTATGTTAGGGTGTTGTGTTCTGGATATCTGGGGCATGACATGGAAGACGACTGCGACATTATTATTATTGGt             >  3:94548/1‑149 (MQ=255)
                                                                                                                                                 cGCAGGTTGTATCTATCGCGGCGAATGATAAGAGTGTGTCGGCGGTCAATTTCCCTTAAGTAACGCTATGTTAGGGTGTTGTGTTCTGGATATCTGGGGCATGACATGGAAGACGACTGCGACATTATTATTATTGGTGCCGGTATTGc  <  3:560943/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
CCTACGAGCGCCTCGATCACCATTATCTCAATGATATTCCAGGTCTGGAAAACCCAACCAGCGAGGTTTTAGCAAAATGGATTTGGGATCAGGTTAAACCCGTTGTGCCGCTGTTAAGTGCGGTGATGGTAAAAGAAACCTGCACCGCAGGTTGTATCTATCGCGGCGAATGATAAGAGTGTGTCGGCGGTCAATTTCCCTTAAGTAACGCTATGTTAGGGTGTTGTGTTCTGGATATCTGGGGCATGACATGGAAGACGACTGCGACATTATTATTATTGGTGCCGGTATTGC  >  NC_000913/2892407‑2892700

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑