Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,046,000 T→A 7.2% intergenic (‑99/+168) ygfF ← / ← gcvP putative NAD(P)‑dependent oxidoreductase/glycine decarboxylase, PLP‑dependent, subunit P of glycine cleavage complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,046,0000TA 7.2% 221.6 / 8.0 69intergenic (‑99/+168)ygfF/gcvPputative NAD(P)‑dependent oxidoreductase/glycine decarboxylase, PLP‑dependent, subunit P of glycine cleavage complex
Reads supporting (aligned to +/- strand):  ref base T (32/32);  new base A (2/3);  total (34/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.71e-01

GTTGCCCGCCCGATGCCGCGACTGCCACCAGTCACAAGTGCTATAGCCATTTGTTTTTCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTA  >  NC_000913/3045852‑3046146
                                                                                                                                                    |                                                                                                                                                  
gTTGCCCGCCCGATGCCGCGACTGCCACCAGTCACAAGTGCTATAGCCATTTGTTTTTCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCttt                                                                                                                                                    <  1:227423/149‑1 (MQ=255)
   gcccgcccGATGCCGCGACTGCCACCAGTCACAAGTGCTATAGCCATTTGTTTTTCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAg                                                                                                                                                 <  4:38697/149‑1 (MQ=255)
   gcccgcccGATGCCGCGACTGCCACCAGTCACAAGTGCTATAGCCATTTGTTTTTCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAg                                                                                                                                                 <  3:112186/149‑1 (MQ=255)
     ccgcccgATGCCGCGACTGCCACCAGTCACAAGTGCTATAGCCATTTGTTTTTCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAggg                                                                                                                                               >  4:157112/1‑149 (MQ=255)
     ccgcccgATGCCGCGACTGCCACCAGTCACAAGTGCTATAGCCATTTGTTTTTCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAggg                                                                                                                                               >  1:324878/1‑149 (MQ=255)
       gcccgATGCCGCGACTGCCACCAGTCACAAGTGCTATAGCCATTTGTTTTTCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGgag                                                                                                                                             <  4:460899/149‑1 (MQ=255)
        cccgATGCCGCGACTGCCACCAGTCACAAGTGCTATAGCCATTTGTTTTTCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGgaga                                                                                                                                            <  1:19180/149‑1 (MQ=255)
          cttcggcgcctttttagtcagatgacaaagtacaaaagtgctcagacagtcccctcgcccctttggggagagggttagggtgaggggaacaggccggcactggcgcgaatatttaccctcaccccggCCCTCGCCCTTTCAGGGAGAgg                                                                                                                                          >  4:229868‑M1/128‑149 (MQ=255)
            aTGCCGCGACTGCCACCAGTCACAAGTGCTATAGCCATTTGTTTTTCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCTCCCTGAAAGGGCGAgggg                                                                                                                                        <  1:40039/149‑2 (MQ=255)
                      tGCCACCAGTCACAAGTGCTATAGCCATTTGTTTTTCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAgg                                                                                                                              <  3:92789/149‑1 (MQ=255)
                      tGCCACCAGTCACAAGTGCTATAGCCATTTGTTTTTCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAgg                                                                                                                              >  4:421441/1‑149 (MQ=255)
                         caccaGTCACAAGTGCTATAGCCATTTGTTTTTCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTa                                                                                                                           >  3:51304/1‑149 (MQ=255)
                          accaGTCACAAGTGCTATAGCCATTTGTTTTTCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTaa                                                                                                                          <  4:520339/149‑1 (MQ=255)
                          accaGTCACAAGTGCTATAGCCATTTGTTTTTCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTaa                                                                                                                          >  3:139079/1‑149 (MQ=255)
                                 acaAGTGCTATAGCCATTTGTTTTTCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTcg                                                                                                                   >  3:584250/1‑149 (MQ=255)
                                    aaagtacaaaagtgctcagacagtcccctcgcccctttggggagagggttagggtgaggggaataggccggcactggcgcgaatatttaccctcaccccggCCCTCGCCCTTTCAGGGagag                                                                                                                                           >  2:486853‑M1/102‑122 (MQ=255)
                                    aaagtacaaaagtgctcagacagtcccctcgcccctttggggagagggttagggtgaggagaataggccggcactggcgcgaatatttaccctcaccccggCCCTCGCCCTTTCAGGGagag                                                                                                                                           <  1:486853‑M1/21‑1 (MQ=255)
                                     gTGCTATAGCCATTTGTTTTTCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGcc                                                                                                               <  3:476503/149‑1 (MQ=255)
                                     gTGCTATAGCCATTTGTTTTTCCGAGAAAAATTAACTCGTTGATGTATCTTTGAGATTGCTAACAAACTGGATCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGcc                                                                                                               >  3:81592/1‑149 (MQ=255)
                                             gCCATTTGTTTTTCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCATTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCgg                                                                                                       <  1:304014/149‑1 (MQ=255)
                                              ccATTTGTTTTTCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGc                                                                                                      >  4:146494/1‑149 (MQ=255)
                                                    gTTTTTCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTc                                                                                                >  1:342710/1‑149 (MQ=255)
                                                    gTTTTTCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTc                                                                                                >  3:553417/1‑149 (MQ=255)
                                                    gTTTTTCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTc                                                                                                >  3:477931/1‑149 (MQ=255)
                                                       tttCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTcccc                                                                                             <  4:584250/149‑1 (MQ=255)
                                                              gaAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTg                                                                                                                                 <  1:87987/106‑1 (MQ=255)
                                                              gaAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTg                                                                                                                                 >  2:87987/1‑106 (MQ=255)
                                                                 aaaTTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGACCGGGGTTAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAAc                                                                                   <  2:506482/149‑1 (MQ=255)
                                                                    ttaattCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTaa                                                                                    <  2:181567/145‑1 (MQ=255)
                                                                    ttaattCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTaa                                                                                    >  1:181567/1‑145 (MQ=255)
                                                                       attCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTTACCCTAACCCtctc                                                                             >  2:162467/1‑149 (MQ=255)
                                                                          cGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTcccc                                                                          <  3:421441/149‑1 (MQ=255)
                                                                           gTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCa                                                                         >  3:308654/1‑149 (MQ=255)
                                                                            ttGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCaa                                                                        >  3:377551/1‑149 (MQ=255)
                                                                                      tttGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAgg                                                                                                                              >  4:457883/1‑85 (MQ=255)
                                                                                      tttGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAgg                                                                                                                              <  3:457883/85‑1 (MQ=255)
                                                                                      tttGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAgg                                                              >  1:104632/1‑149 (MQ=255)
                                                                                      tttGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAgg                                                              >  2:272246/1‑149 (MQ=255)
                                                                                      tttGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAgg                                                              >  4:289246/1‑149 (MQ=255)
                                                                                      tttGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGACAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCGCCCCTTTGGGGAGAgg                                                              <  4:223079/149‑1 (MQ=37)
                                                                                                 tAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGa                                                   <  4:377551/149‑1 (MQ=255)
                                                                                                 tAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGa                                                   <  4:553417/149‑1 (MQ=255)
                                                                                                 tAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGa                                                   <  2:203339/149‑1 (MQ=255)
                                                                                                 tAACAAACTGGCTCAATCTTAAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGa                                                   <  1:578960/149‑1 (MQ=255)
                                                                                                  aaCAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAg                                                  <  2:564027/149‑1 (MQ=255)
                                                                                                   aCAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGc                                                 >  3:363710/1‑149 (MQ=255)
                                                                                                   aCAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGc                                                 >  4:578533/1‑149 (MQ=255)
                                                                                                   aCAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGc                                                 >  2:519086/1‑149 (MQ=255)
                                                                                                      aaCTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACt                                              <  2:110545/149‑1 (MQ=255)
                                                                                                            cTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGga                                                                                                                                              >  3:579157/1‑47 (MQ=255)
                                                                                                            cTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGga                                                                                                                                              <  4:579157/47‑1 (MQ=255)
                                                                                                             tCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTAc                                       >  3:222598/1‑149 (MQ=255)
                                                                                                               aaTCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACtt                                     <  3:146494/149‑1 (MQ=255)
                                                                                                                   ttCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTc                                 <  4:51304/149‑1 (MQ=255)
                                                                                                                   ttCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTc                                 >  2:126835/1‑149 (MQ=255)
                                                                                                                         gTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGa                           <  3:327036/149‑1 (MQ=255)
                                                                                                                          gtGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCGCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGAc                          <  4:81592/148‑1 (MQ=255)
                                                                                                                              cACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCa                                                >  2:50547/1‑123 (MQ=255)
                                                                                                                              cACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCa                                                <  1:50547/123‑1 (MQ=255)
                                                                                                                                tcaccccggCCCTCTCCCTGAAAGGGCGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAggg                                                                    <  2:13973‑M1/92‑1 (MQ=255)
                                                                                                                                tcaccccggCCCTCTCCCTGAAAGGGCGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAggg                                                                    >  1:13973‑M1/10‑101 (MQ=255)
                                                                                                                                cGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGTGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTaaaaa                    <  4:336037/149‑1 (MQ=255)
                                                                                                                                cGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTaaaaa                    <  4:363710/149‑1 (MQ=255)
                                                                                                                                  cTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAgg                  >  3:575351/1‑149 (MQ=255)
                                                                                                                                      cggCCCTCTCCCTGAAAGGGCGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTaaaa                     >  3:449806‑M1/4‑142 (MQ=255)
                                                                                                                                      cggCCCTCTCCCTGAAAGGGCGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTaaaa                     <  4:449806‑M1/139‑1 (MQ=255)
                                                                                                                                         CCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGaa           <  1:279804‑M1/149‑1 (MQ=255)
                                                                                                                                          CCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGGGGGGACTGTCTGAGCCCTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAg          <  3:531029‑M1/149‑1 (MQ=255)
                                                                                                                                             CGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAgcgc       >  3:578533‑M1/1‑149 (MQ=255)
                                                                                                                                                ccTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCtt    >  3:105945/1‑149 (MQ=255)
                                                                                                                                                  tttCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGt                                                       <  2:191997/96‑1 (MQ=255)
                                                                                                                                                  tttCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGt                                                       >  1:191997/1‑96 (MQ=255)
                                                                                                                                                  tttCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTa  <  2:62504/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GTTGCCCGCCCGATGCCGCGACTGCCACCAGTCACAAGTGCTATAGCCATTTGTTTTTCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTA  >  NC_000913/3045852‑3046146

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: