Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,124,929 G→C 10.4% A177A (GCC→GCG)  glcF ← glycolate oxidase 4Fe‑4S iron‑sulfur cluster subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,124,9290GC10.4% 155.8 / 10.0 48A177A (GCC→GCG) glcFglycolate oxidase 4Fe‑4S iron‑sulfur cluster subunit
Reads supporting (aligned to +/- strand):  ref base G (24/19);  new base C (3/2);  total (27/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.89e-01

CCGTGCCAGCCCTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTCAGCAGGCAGTTTTGCTCTGACCTGTTCCGGTAAAAACGGTCGCAGCACCAGCCCTACCTGCGTCAGCGCACGGAA  >  NC_000913/3124785‑3125075
                                                                                                                                                |                                                                                                                                                  
ccGTGCCAGCCCTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGgcgc                                                                                                                                                >  4:1908/1‑149 (MQ=255)
      cAGCCCTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCtt                                                                                                                                          <  4:87340/149‑1 (MQ=255)
       aGCCCTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTc                                                                                                                                         <  4:559956/149‑1 (MQ=255)
        gtccTTTCTCCTGCGCATTAAGATGATAGTCCACAGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGTTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTcc                                                                                                                                        <  2:290898/147‑1 (MQ=255)
         cccTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGCGCCCAGCCtacgc                                                                                                                                       <  1:25840/149‑5 (MQ=255)
          ccTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCaa                                                                                                                                      <  4:453729/149‑1 (MQ=255)
          ccTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGCGCCCAGCCtacg                                                                                                                                        <  3:219044/147‑4 (MQ=255)
          ccTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGCGCCCAGCCtacg                                                                                                                                        >  4:219044/1‑144 (MQ=255)
                 ccTGCGCATTAAGATGATAGTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTaaa                                                                                                                               <  4:122719/149‑1 (MQ=255)
                   tGCGCATTAAGATGATAGTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAAc                                                                                                                             >  3:242770/1‑149 (MQ=255)
                        aTTAAGATGATAGTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGAc                                                                                                                        >  3:18168/1‑149 (MQ=255)
                             gatgatAGTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTa                                                                                                                   >  4:390059/1‑149 (MQ=255)
                                    gTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTGATGGCGCa                                                                                                            >  2:485806/1‑149 (MQ=255)
                                     tCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACAtt                                                                                                                                  <  3:277845/126‑1 (MQ=255)
                                     tCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACAtt                                                                                                                                  >  4:277845/1‑126 (MQ=255)
                                       cACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAgcg                                                                                                         >  1:424274/1‑149 (MQ=255)
                                                acaacaGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGAcgcg                                                                                                >  3:456384/1‑149 (MQ=255)
                                                       ccTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGc                                                                                         >  2:504236/1‑149 (MQ=255)
                                                          gCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCttt                                                                                      >  4:134528/1‑149 (MQ=255)
                                                            ttCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCa                                                                                    >  1:432036/1‑149 (MQ=255)
                                                            ttCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCa                                                                                    <  2:186218/149‑1 (MQ=255)
                                                            atCGTTAGCTGGCATGACGCTGAGCCCGAGACGATCCAGCACTCGCGCAGTTGCCTCGTTGTTGTTGGGCCAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGTCGCAGCGGCGGACGCGGTTTAGCTGTCa                                                                                    >  2:293436/2‑149 (MQ=255)
                                                               gTTAGCTGGCGTGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTCCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCg                                                                                 >  4:136621/1‑149 (MQ=255)
                                                                ttAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGt                                                                                <  2:329374/149‑1 (MQ=255)
                                                                 tAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTACCTTTCACCGtt                                                                               <  1:485806/149‑1 (MQ=255)
                                                                          tGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTCAgcaggc                                                                      >  4:254000/1‑149 (MQ=255)
                                                                              gCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTCAGCAGGCAGtt                                                                  >  2:93259/1‑149 (MQ=255)
                                                                               cTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTCAGCAGGCAGttt                                                                 <  3:582990/149‑1 (MQ=255)
                                                                                 gATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTCAGCAGGCAGTTTTg                                                               >  1:97403/1‑149 (MQ=255)
                                                                                  aTCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTCAGCAGGCAGTTTTGc                                                              <  4:162191/149‑1 (MQ=255)
                                                                                     cccAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTCAGCAGGCAGTTTTGctct                                                           >  1:21138/1‑149 (MQ=255)
                                                                                      ccAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTCAGCAGGCAGTTTTGCTCTg                                                          >  3:320980/1‑149 (MQ=255)
                                                                                        aGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTCAGCAGGCAGTTTTGCTCTGAc                                                        <  4:320980/149‑1 (MQ=255)
                                                                                        aGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTCAGCAGGCAGTTTTGCTCTGAc                                                        <  2:482746/149‑1 (MQ=255)
                                                                                        aGACGATCCAGCACACGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCATCGGCGGACGCGGTTTAGCTTTCACCGTTTCAGCAGGCAGTTTTGCTCTGAc                                                        <  2:509955/149‑1 (MQ=255)
                                                                                                        gcgcAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTCAGCAGGCAGTTTTGCTCTGACCTGTTCCGGTAAAAAc                                        >  2:581639/1‑149 (MQ=255)
                                                                                                           cAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTCAGCAGGCAGTTTTGCTCTGACCTGTTCCGGTAAAAACGGt                                     <  4:18168/149‑1 (MQ=255)
                                                                                                           cAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTCAGCAGGCAGTTTTGCTCTGACCTGTTCCGGTAAAAACGGt                                     <  3:134528/149‑1 (MQ=255)
                                                                                                              ttGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGCCGGAGCCTTGCAACATTAAAACCCGAAGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTCAGCAGGCAGTTTTGCTCTGACCTGTTCCGGTAAAAACGGTCgc                                  >  4:285285/1‑149 (MQ=255)
                                                                                                                         gtgtTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTCAGCAGGCAGTTTTGCTCTGACCTGTTCCGGTAAAAACGGTCGCAGCACCAGccc                       <  1:93259/149‑1 (MQ=255)
                                                                                                                           gtTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGTGGTTTAGCTTTCACCGTTTCAGCAGGCAGTTTTGCTCTGACCTGTTCCGGTAAAAACGGTCGCAGCACCAGCCCTa                     >  4:120232/1‑149 (MQ=255)
                                                                                                                                  gAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTcag                                                                           >  4:224337/1‑88 (MQ=255)
                                                                                                                                  gAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTcag                                                                           <  3:224337/88‑1 (MQ=255)
                                                                                                                                  gAAAGCGTAGGCTGGGCGCAGCCTTACAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTCAGCAGGCAGTTTTGCTCTGACCTGTTCCGGTAAAAACGGTCGCAGCACCAGCCCTACCTGCGt              >  3:493687/1‑149 (MQ=255)
                                                                                                                                  gAAAGCGTAGGCTGGGCGAAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTCAGCAGGCAGTTTTGCTCTGACCTGTTCCGGTAAAAACGGTCGCAGCACCAGCCCTACCTGCGt              >  2:207864/1‑149 (MQ=255)
                                                                                                                                       ggaaGGCTGCGCCCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTCAGCAGGCAGTTTTGCTCTGACCTGTTCCGGTAAAAACGGTCGCAGCACCAGCCCTACCTGCGTCAgcg         >  2:287578/4‑149 (MQ=255)
                                                                                                                                         aaGGCTGCGCCCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTCAGCAGGCAGTTTTGCTCTGACCTGTTCCGGTAAAAACGGTCGCAGCACCAGCCCTACCTGCGTCAGCGCa       >  4:344246/2‑149 (MQ=255)
                                                                                                                                              tGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTCAGCAGGCAGTTTTGCTCTGACCTGTTCCGGTAAAAACGGTCGCAGCACCAGCCCTACCTGCGTCAGCGCACGGaa  <  2:135119/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
CCGTGCCAGCCCTTTCTCCTGCGCATTAAGATGATAGTCCACCGCGCCACAACAGCCTGCTTCGTTAGCTGGCATGACGCTGATCCCCAGACGATCCAGCACTCGCGCAGTTGCCGCGTTGGTGTTGGGCGAAAGCGTAGGCTGGGCGCAGCCTTCCAACATTAAAACCCGACGCTTATGGCGCAGCGGCGGACGCGGTTTAGCTTTCACCGTTTCAGCAGGCAGTTTTGCTCTGACCTGTTCCGGTAAAAACGGTCGCAGCACCAGCCCTACCTGCGTCAGCGCACGGAA  >  NC_000913/3124785‑3125075

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑