Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,192,140 G→T 100% intergenic (‑201/‑68) glgS ← / → yqiJ motility and biofilm regulator/DUF1449 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,192,1400GT97.3% 264.9 / ‑0.1 74intergenic (‑201/‑68)glgS/yqiJmotility and biofilm regulator/DUF1449 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (39/33);  total (40/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.63e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GTATTCCCCAATCAGTGTAGTGCCTTTAAACATAACGTGCGTAAATATATTGTCCCCGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACACCCCTTATCTTTTCGCTATTTCCTTTGTACTGTTGATCGGCCTGTTAGAAA  >  NC_000913/3191997‑3192283
                                                                                                                                               |                                                                                                                                               
gTATTCCCCAATCAGTGTAGTGCCTTTAAACATAACGTGCGTAAATATATTGTCCCCGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACATATaaa                                                                                                                                            >  2:541946/1‑149 (MQ=255)
gTATTCCCCAATCAGTGTAGTGCCTTTAAACATAACGTGCGTAAATATATTGTCCCCGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACATATaaa                                                                                                                                            <  3:415041/149‑1 (MQ=255)
 tATTCCCCAATCAGTGTAGTGCCTTTAAACATAACGTGCGTAAATATATTGTCCCCGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACATATAAAt                                                                                                                                           >  1:212548/1‑149 (MQ=255)
     ccccAATCAGTGTAGTGCCTTTAAACATAACGTGCGTAAATATATTGTCCCCGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACATATAAATGTaa                                                                                                                                       >  1:433201/1‑149 (MQ=255)
     ccccAATCAGTGTAGTGCCTTTAAACATAACGTGCGTAAATATATTGTCCCCGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACATATAAATGTaa                                                                                                                                       <  2:80442/149‑1 (MQ=255)
        cAATCAGTGTAGTGCCTTTAAACATAACGTGCGTAAATATATTGTCCCCGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACATATAAAt                                                                                                                                           <  2:391596/142‑1 (MQ=255)
        cAATCAGTGTAGTGCCTTTAAACATAACGTGCGTAAATATATTGTCCCCGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACATATAAAt                                                                                                                                           >  1:391596/1‑142 (MQ=255)
        cAATCAGTGTAGTGCCTTTAAACATAACGTGCGTAAATATATTGTCCCCGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACATATAAATGTAAGCa                                                                                                                                    >  4:550445/1‑149 (MQ=255)
         aaTCAGTGTAGTGCCTTTAAACATAACGTGCGTAAATATATTGTCCCCGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACATATAAATGTAAGCaa                                                                                                                                   <  3:45335/149‑1 (MQ=255)
          aTCAGTGTAGTGCCTTTAAACATAACGTGCGTAAATATATTGTCCCCGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACATATAAATGTAAGCAAg                                                                                                                                  >  1:536516/1‑149 (MQ=255)
                        tttAAACATAACGTGCGTAAATATATTGTCCCCGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACATATAAATGTAAGCAAGGAAACTATTACaaa                                                                                                                    >  2:172956/1‑149 (MQ=255)
                        tttAAACATAACGTGCGTAAATATATTGTCCCCGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACATATAAATGTAAGCAAGGAAACTATTACaaa                                                                                                                    <  2:536516/149‑1 (MQ=255)
                            aaCATAACGTGCGTAAATATATTGTCCCCGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACATATAAATGTAAGCAAGGAAACTATTACAAAGTaa                                                                                                                <  4:71631/149‑1 (MQ=255)
                                 aaCGTGCGTAAATATATTGTCCCCGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACATATAAATGTAAGCAAGGAAACTATTACAAAGTAAATAtt                                                                                                           >  2:220834/1‑149 (MQ=255)
                                        gTAAATATATTGTCCCCGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACATATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCAc                                                                                                    >  2:8848/1‑149 (MQ=255)
                                         tAAATATATTGTCCCCGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACATATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACt                                                                                                   >  1:497674/1‑149 (MQ=255)
                                             tatatTGTCCCCGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACATATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAAt                                                                                               <  4:73011/149‑1 (MQ=255)
                                                atTGTCCCCGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACATATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGa                                                                                            <  3:406869/149‑1 (MQ=255)
                                                 ttGTCCCCGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACATATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGAc                                                                                           >  2:525465/1‑149 (MQ=255)
                                                     ccacGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACATATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACACTg                                                                                       <  3:389055/146‑1 (MQ=255)
                                                       ccGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACATATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGa                                                                                     >  2:377387/1‑149 (MQ=255)
                                                          aTCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACATATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTg                                                                                  >  1:472587/1‑149 (MQ=255)
                                                             acacTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACATATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACAtt                                                                                        >  3:104950/1‑140 (MQ=255)
                                                             acacTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACATATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACAtt                                                                                        <  4:104950/140‑1 (MQ=255)
                                                             acacTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACATATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGata                                                                               >  2:273929/1‑149 (MQ=255)
                                                              cacTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACATATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGatat                                                                              <  1:172956/149‑1 (MQ=255)
                                                                  ttttAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACATATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGa                                                                          <  3:377219/149‑1 (MQ=255)
                                                                  ttttAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACATATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGa                                                                          <  3:257758/149‑1 (MQ=255)
                                                                  ttttAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACATATAAATGTAAGCAAGGAAACTATTAAAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGa                                                                          <  4:342449/149‑1 (MQ=255)
                                                                       gTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACATATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTa                                                                     <  1:398177/149‑1 (MQ=255)
                                                                        tGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACATATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTAt                                                                    >  3:549055/1‑149 (MQ=255)
                                                                         gAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACATATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGa                                                                                            >  2:276089/1‑124 (MQ=255)
                                                                         gAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACATATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGa                                                                                            <  1:276089/124‑1 (MQ=255)
                                                                          aaaaaTAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACATATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTc                                                                  >  1:11989/1‑149 (MQ=255)
                                                                          aaaaaTAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACATATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTc                                                                  <  2:172707/149‑1 (MQ=255)
                                                                            aaaTAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACATATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGc                                                                >  1:415125/1‑149 (MQ=255)
                                                                            aaaTAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACATATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGc                                                                <  1:158182/149‑1 (MQ=255)
                                                                                    aaCCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACATATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACtata                                                        >  3:169905/1‑149 (MQ=255)
                                                                                    aaCCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACATATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACtata                                                        >  1:398238/1‑149 (MQ=255)
                                                                                     aCCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACATATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATaa                                                       <  1:377387/149‑1 (MQ=255)
                                                                                       cATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACATATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAaca                                                     >  3:457539/1‑149 (MQ=255)
                                                                                       cATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACATATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACAAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAaca                                                     >  2:28468/1‑149 (MQ=255)
                                                                                        atatTAAAAGACTTTCTCTTCAAGAACTTCTCTAACCTGTGTTATTATTAACACATATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAacac                                                    >  1:133283/1‑149 (MQ=255)
                                                                                             aaaaGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAATACATATAAATGTAAGCAAGGAAACTATTACAAAg                                                                                                                   <  3:389716/81‑1 (MQ=255)
                                                                                             aaaaGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACATATAAATGTAAGCAAGGAAACTATTACAAAg                                                                                                                   >  4:389716/1‑81 (MQ=255)
                                                                                             aaaaGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACATATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGa                                                                                     >  1:287454/1‑111 (MQ=255)
                                                                                             aaaaGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACATATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGa                                                                                     <  2:287454/111‑1 (MQ=255)
                                                                                             aaaaGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACATATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACACCCCtt                                               >  2:169859/1‑149 (MQ=255)
                                                                                              aaaGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACATATAAATGTAAGAAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACACCCCTTa                                              >  4:429413/1‑149 (MQ=255)
                                                                                              aaaGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGt                                                                                                       >  1:333323/1‑92 (MQ=255)
                                                                                              aaaGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGt                                                                                                       <  2:333323/92‑1 (MQ=255)
                                                                                               aaGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACATATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACACCCCTTAt                                             >  4:100545/1‑149 (MQ=255)
                                                                                                  aCTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACATATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACACCCCTTATCtt                                          >  2:532384/1‑149 (MQ=255)
                                                                                                     ttCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACATATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACACCCCTTATCTTTTc                                       <  2:497674/149‑1 (MQ=255)
                                                                                                     ttCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACATATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACACCCCTTATCTTTTc                                       <  2:322216/149‑1 (MQ=255)
                                                                                                     ttCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACATATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACACCCCTTATCTTTTc                                       <  3:491681/149‑1 (MQ=255)
                                                                                                          ttCCAGAACTTCTCTAACCTGTGTTATTAGTAACACATATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACACCCCTTATCTTTTCGCTAt                                  >  3:501988/1‑149 (MQ=255)
                                                                                                               gAACTTCTCTAACCTGTGTTATTATTAACACATATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACACCCCTTATCTTTTCGCTATTTCCt                             <  1:388891/149‑1 (MQ=255)
                                                                                                                aaCTTCTCTAACCTGTGTTATTATTAACACATATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACACCCCTTATCTTTTCGCTATTTCCtt                            <  3:448261/149‑1 (MQ=255)
                                                                                                                 aCTTCTCTAACCTGTGTTATTATTAACACATATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACACCCCTTATCTTTTCGCTATTTCCttt                           <  1:312474/149‑1 (MQ=255)
                                                                                                                 aCTTCTCTAACCTGTGTTATTATTAACACATATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACACCCCTTATCTTTTCGCTATTTCCttt                           <  1:525465/149‑1 (MQ=255)
                                                                                                                    tctcTAACCTGTGTTATTATTAACACATATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACACCCCTTATCTTTTCGCTATTTCCTTTGTa                        <  2:11989/149‑1 (MQ=255)
                                                                                                                      tctAACCTGTGTTATTATTAACACATATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACACCCCTTATCTTTTCGCTATTTCCTTTGTACt                      <  2:538590/149‑1 (MQ=255)
                                                                                                                      tctAACCTGTGTTATTATTAACACATATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACACCCCTTATCTTTTCGCTATTTCCTTTGTACt                      >  3:34492/1‑149 (MQ=255)
                                                                                                                       ctAACCTGTGTTATTATTAACACATATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACACCCCTTATCTTTTCGCTATTTCCTTTGTACTg                     <  2:212548/149‑1 (MQ=255)
                                                                                                                             tgtgTTATTATTAACACATATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACACCCCTTATCTTTTCGCTATTTCCTTTGTACTGTTGATc               >  3:308258/1‑149 (MQ=255)
                                                                                                                                 ttattattaACACATATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACACCCCTTATCTTTTCGCTATTTCCTTTGTACTGTTGATCGGcc           >  3:130768/1‑149 (MQ=255)
                                                                                                                                  tattattaACAGATATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACACCCCTTATCTTTTCGCTATTTCCTTTGTACTGTTGATCGGc            >  1:311351/1‑147 (MQ=255)
                                                                                                                                  tattattaACACATATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACACCCCTTATCTTTTCGCTATTTCCTTTGTACTGTTGATCGGc            <  2:311351/147‑1 (MQ=255)
                                                                                                                                   attattaACACATATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACACCCCTTATCTTTTCGCTATTTCCTTTGTACTGTTGATCGGCCTg         >  4:120796/1‑149 (MQ=255)
                                                                                                                                    ttattaACACATATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACACCCCTTATCTTTTCGCTATTTCCTTTGTACTGTTGATCGGCCTGt        <  4:308258/149‑1 (MQ=255)
                                                                                                                                       ttaACACATATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACACCCCTTATCTTTTCGCTATTTCCTTTGTACTGTTGATCGGCCTGTTAg     >  2:529659/1‑149 (MQ=255)
                                                                                                                                          acacATATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACACCCCTTATCTTTTCGCTATTTCCTTTGTACTGTTGATCGGCCTGTTAGaaa  >  3:496577/1‑149 (MQ=255)
                                                                                                                                          acacATATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTAGAGTGATATATGATTTTATTCGCCGACTATAACACCCCTTATCTTTTCGCTATTTCCTTTGTACTGTTGATCGGCCTGTTAGaaa  >  1:573750/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                               
GTATTCCCCAATCAGTGTAGTGCCTTTAAACATAACGTGCGTAAATATATTGTCCCCGATCACACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGAGTGATATATGATTTTATTCGCCGACTATAACACCCCTTATCTTTTCGCTATTTCCTTTGTACTGTTGATCGGCCTGTTAGAAA  >  NC_000913/3191997‑3192283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑