Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,488,989 G→T 6.6% H398N (CAC→AAC)  argD ← bifunctional acetylornithine aminotransferase and succinyldiaminopimelate aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,488,9890GT 6.6% 206.2 / 3.1 60H398N (CAC→AAC) argDbifunctional acetylornithine aminotransferase and succinyldiaminopimelate aminotransferase
Reads supporting (aligned to +/- strand):  ref base G (30/26);  new base T (2/2);  total (32/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

GACCGCATCACGGGATTTGGCTGAGTCGCAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGAAATCACGCGCCCGACCTTTGTACTGT  >  NC_000913/3488841‑3489137
                                                                                                                                                    |                                                                                                                                                    
gACCGCATCACGGGATTTGGCTGAGTCGCAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTg                                                                                                                                                      <  3:274267/149‑1 (MQ=255)
gACCGCATCACGGGATTTGGCTGAGTCGCAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTg                                                                                                                                                      <  3:36466/149‑1 (MQ=255)
 aCCGCATCACGGGATTTGGCTGAGTCGCAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTgg                                                                                                                                                     <  2:63032/149‑1 (MQ=255)
 aCCGCATCACGGGATTTGGCTGAGTCGCAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTgg                                                                                                                                                     <  2:142383/149‑1 (MQ=255)
 aCCGCATCACGGGATTTGGCTGAGTCGCAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTgg                                                                                                                                                     >  1:74815/1‑149 (MQ=255)
         aCGGGATTTGGCTGAGTCGCAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCCACGCGGTGGCGaaggt                                                                                                                                             <  1:293925/149‑4 (MQ=255)
         aCGGGATTTGGCTGAGTCGCAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACg                                                                                                                                             >  2:331336/1‑149 (MQ=255)
          cGGGATTTGGCTGAGTCGCAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCCACGCGGTGGCGaaggtg                                                                                                                                            <  4:294159/149‑5 (MQ=255)
          cGGGATTTGGCTGAGTCGCAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGt                                                                                                                                            <  1:531030/149‑1 (MQ=255)
           gggATTTGGCTGAGTCGCAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGtt                                                                                                                                           >  1:253620/1‑149 (MQ=255)
           gggATTTGGCTGAGTCGCAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGtt                                                                                                                                           <  1:155554/149‑1 (MQ=255)
                        gTCGCAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATc                                                                                                                              <  4:240510/149‑1 (MQ=255)
                        gTCGCAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATc                                                                                                                              <  2:74815/149‑1 (MQ=255)
                         tCGCAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTg                                                                                                                                          >  4:167532/1‑136 (MQ=255)
                         tCGCAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTg                                                                                                                                          <  3:167532/136‑1 (MQ=255)
                         tCGCAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCg                                                                                                                             >  4:349190/1‑149 (MQ=255)
                           gCAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGat                                                                                                                           <  4:99763/149‑1 (MQ=255)
                             aaGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGatat                                                                                                                         >  2:549990/1‑149 (MQ=255)
                                 tGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGc                                                                                                                     <  4:289224/149‑1 (MQ=255)
                                              ggCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTccaccacc                                                                                                        <  1:380059/149‑1 (MQ=255)
                                                   aaTGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGa                                                                                                   <  2:183993/149‑1 (MQ=255)
                                                      gCAGGCTAGACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACgg                                                                                                <  2:20432/149‑1 (MQ=255)
                                                       cAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGt                                                                                               <  4:418054/149‑1 (MQ=255)
                                                             aaaCCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCaaaa                                                                                         <  2:596044/149‑1 (MQ=255)
                                                              aaCCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAAc                                                                                        >  2:126697/1‑149 (MQ=255)
                                                               aCCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACg                                                                                       >  3:520744/1‑149 (MQ=255)
                                                                 cATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCCACGCGGTGGCGaa                                                                                                                                                >  4:249150/1‑90 (MQ=38)
                                                                 cATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCa                                                                                     >  3:38322/1‑149 (MQ=255)
                                                                  aTCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCAt                                                                                    >  3:282405/1‑149 (MQ=255)
                                                                      gATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATcaca                                                                                >  1:153186/1‑149 (MQ=255)
                                                                      gATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATcaca                                                                                >  4:450080/1‑149 (MQ=255)
                                                                        tAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACAtc                                                                              <  3:455153/149‑1 (MQ=255)
                                                                                  aCGCCGCATCCGACATCTTTTGCCTGAGGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCa                                                                    >  2:434872/1‑149 (MQ=255)
                                                                                     ccgcATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTc                                                                 <  4:282405/149‑1 (MQ=255)
                                                                                      cgcATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGc                                                                                                                     >  4:526101/1‑96 (MQ=255)
                                                                                      cgcATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGc                                                                                                                     <  3:526101/96‑1 (MQ=255)
                                                                                             gACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCAGCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCAt                                                         >  3:482527/1‑149 (MQ=255)
                                                                                             gACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCAt                                                         >  3:298377/1‑149 (MQ=255)
                                                                                                   ttttGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACAtccggtccgg                                                                      >  2:257049/1‑130 (MQ=255)
                                                                                                   ttttGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACAtccggtccgg                                                                      <  1:257049/130‑1 (MQ=255)
                                                                                                   ttttGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGcc                                                   >  3:326484/1‑149 (MQ=255)
                                                                                                         cTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCctc                                             <  3:450080/149‑1 (MQ=255)
                                                                                                         cTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCctc                                             >  3:583549/1‑149 (MQ=255)
                                                                                                          tGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCctct                                            >  1:501628/1‑149 (MQ=255)
                                                                                                          tGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCctct                                            >  3:581435/1‑149 (MQ=255)
                                                                                                                     attgaggCCCCAACCACCTTCGCCACCCCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCAtt                                 <  4:506369/143‑2 (MQ=255)
                                                                                                                        tACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAg                              <  1:549990/149‑1 (MQ=255)
                                                                                                                            ccccAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGaaa                          >  3:353049/1‑149 (MQ=255)
                                                                                                                             cccAACCACCTTCGCCACCGCGTGGGAGAAACGTTGCAGCCCTTCATCGATATCCGCATCTTCCACTACCAGCGACGGTGCAAAACGCATCACATCCGATCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGAAAt                         >  3:363501/1‑149 (MQ=255)
                                                                                                                              ccAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCCTACAGGAAATc                        <  1:443452/149‑1 (MQ=255)
                                                                                                                              ccAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGAAATc                        >  3:446769/1‑149 (MQ=255)
                                                                                                                               cAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGAAATCa                       >  1:280128/1‑149 (MQ=255)
                                                                                                                               cAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGAAATCa                       <  4:520744/149‑1 (MQ=255)
                                                                                                                                aaCCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGAAATCAc                      >  1:598003/1‑149 (MQ=255)
                                                                                                                                 accaccTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGAAATCAcg                     >  4:206454/1‑149 (MQ=255)
                                                                                                                                   caccTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGAAATCAcgcg                   >  4:247780/1‑149 (MQ=255)
                                                                                                                                    accTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGAAATCACgcgc                  >  2:375596/1‑149 (MQ=255)
                                                                                                                                      tttCGCCCACGCGGTGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGGCCGGCATTCAGCACCAGTACGCCAGCCTCTGCGCCCGCATACAGGAAATCACGCGccc                >  2:564984/2‑149 (MQ=255)
                                                                                                                                       ttCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGAAATCACGCGCCCg               >  3:287751/1‑149 (MQ=255)
                                                                                                                                           ccACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGAAATCACGCGCCCGACCt           <  4:298377/149‑1 (MQ=255)
                                                                                                                                             aCCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGAAATCACGCGCCCGACCttt         >  3:240682/1‑149 (MQ=255)
                                                                                                                                             aCCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGAAATCACGCGCCCGACCttt         <  3:296555/149‑1 (MQ=255)
                                                                                                                                              ccGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGAAATCACGCGCCCGACCTTTg        <  2:598003/149‑1 (MQ=255)
                                                                                                                                              ccGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGAAATCACGCGCCCGACCTTTg        <  2:501628/149‑1 (MQ=255)
                                                                                                                                              ccGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGAAATCACGCGCCCGACCTTTg        <  4:581435/149‑1 (MQ=255)
                                                                                                                                                    gggCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGAAATCACGCGCCCGACCTTTGTACtgt  <  4:583549/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GACCGCATCACGGGATTTGGCTGAGTCGCAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGAAATCACGCGCCCGACCTTTGTACTGT  >  NC_000913/3488841‑3489137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑