Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,853,603 A→G 11.6% A17A (GCA→GCG) ‡ tisB → toxic membrane persister formation peptide, LexA‑regulated

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,853,6030AG11.6% 161.2 / 10.7 60A17A (GCA→GCG) ‡tisBtoxic membrane persister formation peptide, LexA‑regulated
Reads supporting (aligned to +/- strand):  ref base A (25/28);  new base G (4/3);  total (29/31)
Fisher's exact test for biased strand distribution p-value = 7.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01

CAACGTTTCTCCTTGAGATACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCC  >  NC_000913/3853456‑3853743
                                                                                                                                                   |                                                                                                                                            
caACGTTTCTCCTTGAGATACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCAc                                                                                                                                             <  2:194384/149‑1 (MQ=255)
 aaCGTTTCTCCTTGAGATACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACt                                                                                                                                            >  2:90735/1‑149 (MQ=255)
    gTTTCTCCTTGAGATACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCa                                                                                                                                         >  4:24777/1‑149 (MQ=255)
         tcCTTGAGATACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGc                                                                                                                                    >  3:279843/1‑149 (MQ=255)
           cTTGAGATACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGTGCTGCAAcaatga                                                                                                                                  <  1:194585/149‑6 (MQ=255)
           cTTGAGATACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCtt                                                                                                                                  >  3:24636/1‑149 (MQ=255)
            ttGAGATACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTg                                                                                                                                 >  4:546391/1‑149 (MQ=255)
              gagaTACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGAt                                                                                                                               <  4:279843/149‑1 (MQ=255)
              gagaTACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGAt                                                                                                                               <  3:207824/149‑1 (MQ=255)
                      gcgTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCTGGTGGATAACGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCt                                                                                                                       >  2:455820/1‑149 (MQ=255)
                              aacaGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCTGGTGGATATCGCCATTCTTGTCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATAcc                                                                                                               >  1:103655/1‑149 (MQ=255)
                               acaGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCt                                                                                                              <  2:150930/149‑1 (MQ=255)
                                caGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTg                                                                                                             >  4:65312/1‑149 (MQ=255)
                                  gCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGaa                                                                                                           <  4:382152/149‑1 (MQ=255)
                                  gCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGaa                                                                                                           <  3:132053/149‑1 (MQ=255)
                                       cAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAAt                                                                                                      <  2:103655/149‑1 (MQ=255)
                                          cAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCa                                                                                                   <  1:90735/149‑1 (MQ=255)
                                          cAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCa                                                                                                   <  1:61957/149‑1 (MQ=255)
                                            ggCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGa                                                                                                 <  3:546391/149‑1 (MQ=255)
                                             gCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGTGCTGCaa                                                                                                                                        >  1:243323/1‑109 (MQ=255)
                                             gCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGTGCTGCaa                                                                                                                                        <  2:243323/109‑1 (MQ=255)
                                                    aaaGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTc                                                                                         <  2:469613/149‑1 (MQ=255)
                                                       ggTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCa                                                                                      <  4:393653/149‑1 (MQ=255)
                                                       ggTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCa                                                                                      <  3:404392/149‑1 (MQ=255)
                                                         tACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCAcc                                                                                    >  4:392621/1‑149 (MQ=255)
                                                            gTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCaaa                                                                                 >  2:403555/1‑149 (MQ=255)
                                                              cAGCTGGCAGTGCTCCTCAACCACAGGAGACGCGTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAgg                                                                               <  1:379257/149‑1 (MQ=255)
                                                               aGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAggg                                                                              <  4:24636/149‑1 (MQ=255)
                                                                    gCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCaa                                                                                            <  1:283434/130‑1 (MQ=255)
                                                                    gCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCaa                                                                                            >  2:283434/1‑130 (MQ=255)
                                                                         gCTCCTGAACCACAGGAGACGCGTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGaaa                                                                    <  1:79441/149‑1 (MQ=255)
                                                                             cTGAACCACAGGAGACGCGTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAAtt                                                                                                     >  4:112125/1‑112 (MQ=255)
                                                                             cTGAACCACAGGAGACGCGTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAAtt                                                                                                     <  3:112125/112‑1 (MQ=255)
                                                                             cTGAACCACAGGAGACGCGTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGc                                                                >  4:154178/1‑149 (MQ=255)
                                                                               gAACCACAGGAGACGCGTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGTGCTGCAAcaa                                                                                                                                     <  1:44740/78‑3 (MQ=255)
                                                                               gAACCACAGGAGACGCGTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGTGCTGCAAcaa                                                                                                                                     >  2:44740/1‑76 (MQ=255)
                                                                                  ccACAGGAGACGCGTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGTTTCCCGCTcccc                                                           <  2:400655/149‑1 (MQ=255)
                                                                                  ccACAGGAGACGCGTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTcccc                                                           <  2:141307/149‑1 (MQ=255)
                                                                                    acaGGAGACGCGTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCtt                                                         <  3:65312/149‑1 (MQ=255)
                                                                                     caGGAGATGCGTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATAcc                                                                                                               >  3:231055/1‑94 (MQ=255)
                                                                                     caGGAGATGCGTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATAcc                                                                                                               <  4:231055/94‑1 (MQ=255)
                                                                                        gagaCGCGTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTtat                                                     <  3:154178/149‑1 (MQ=255)
                                                                                             gcgTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTa                                                >  2:503508/1‑149 (MQ=255)
                                                                                                        tggtggATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGtt                                     >  2:300195/1‑149 (MQ=255)
                                                                                                           tggATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGgt                                  >  1:503009/1‑149 (MQ=255)
                                                                                                            ggATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGgtg                                 >  1:507813/1‑149 (MQ=255)
                                                                                                                atCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTc                                                                                                                        >  2:48609/1‑58 (MQ=255)
                                                                                                                atCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTc                                                                                                                        <  1:48609/58‑1 (MQ=255)
                                                                                                                atCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCgg                             >  2:583611/1‑149 (MQ=255)
                                                                                                                   gCCATTCTTATCCTCAAGCTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTACTTCAGATTCAAGTCGCACCAAAGGGGAGCGGGaaa                                                                    <  3:562590/107‑1 (MQ=255)
                                                                                                                   gCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGaaa                                                                    >  4:562590/1‑107 (MQ=255)
                                                                                                                   gCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGgatg                          >  2:538530/1‑149 (MQ=255)
                                                                                                                   gCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGgatg                          >  1:465656/1‑149 (MQ=255)
                                                                                                                            aTCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGa                 <  3:24777/149‑1 (MQ=255)
                                                                                                                               cTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGTTTCCCGCTCCCCttt                                                        >  3:532970/1‑107 (MQ=39)
                                                                                                                               cTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGTTTCCCGCTCCCCttt                                                        <  4:532970/107‑1 (MQ=39)
                                                                                                                                     cTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCg                                         <  2:357757/116‑1 (MQ=255)
                                                                                                                                     cTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAACTCGCACCAAAGAGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCg                                         >  1:357757/1‑116 (MQ=255)
                                                                                                                                        gcagttgCAGTGCTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATcc     >  4:29664/4‑149 (MQ=255)
                                                                                                                                           gttgCAGTGCTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGcc  >  1:545758/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                            
CAACGTTTCTCCTTGAGATACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCC  >  NC_000913/3853456‑3853743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑