Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,912,781 A→G 6.3% I296I (ATT→ATC)  glmS ← L‑glutamine:D‑fructose‑6‑phosphate aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,912,7810AG 6.3% 196.6 / 3.5 64I296I (ATT→ATC) glmSL‑glutamine:D‑fructose‑6‑phosphate aminotransferase
Reads supporting (aligned to +/- strand):  ref base A (29/30);  new base G (2/2);  total (31/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.56e-01

CATCAGGCTGTTACGACGCACGGCAGATTTGCGATAGCGGAATTCAGAGGCGATTTCGACGTCGCACGGAATACCTGCTAGCGATTCAAACCAGTAGCGGGAAACCATACCGGAGTTATAAGAAGTACCACAGGCGAGGATCTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATG  >  NC_000913/3912637‑3912928
                                                                                                                                                |                                                                                                                                                   
catcaGGCTGTTACGACGCACGGCAGATTTGCGATAGCGGAATTCAGAGGCGATTTCGACGTCGCACGGAATACCTGCTAGCGATTCAAACCAGTAGCGGGAAACCATACCGGAGTTATAAGAAGTACCACAGGCGAGGATCTGAatat                                                                                                                                                 <  1:14564/149‑1 (MQ=255)
catcaGGCTGTTACGACGCACGGCAGATTTGCGATAGCGGAATTCAGAGGCGATTTCGACGTCGCACGGAATACCTGCTAGCGATTCAAACCAGTAGCGGGAAACCATACCGGAGTTATAAGAAGTACCACAGGCGAGGATCTGAatat                                                                                                                                                 <  4:508535/149‑1 (MQ=255)
 atcaGGCTGTTACGACGCACGGCAGATTTGCGATAGCGGAATTCAGAGGCGATTTCGACGTCGCACGGAATACCTGCTAGCGATTCAAACCAGTAGCGGGAAGCCATACCGGAGTTATAAGAAGTACCACAGGCGAGGATCTGAATATg                                                                                                                                                >  4:243550/1‑149 (MQ=255)
  tcaGGCTGTTACGACGCACGGCAGATTTGCGATAGCGGAATTCAGAGGCGATTTCGACGTCGCACGGAATACCTGCTAGCGATTCAAACCAGTAGCGGGAAACCATACCGGAGTTATAAGAAGTACCACAGGCGAGGATCTGAATATGc                                                                                                                                               >  4:406975/1‑149 (MQ=255)
  tcaGGCTGTTACGACGCACGGCAGATTTGCGATAGCGGAATTCAGAGGCGATTTCGACGTCGCACGGAATACCTGCTAGCGATTCAAACCAGTAGCGGGAAACCATACCGGAGTTATAAGAAGTACCACAGGCGAGGATCTGAATATGc                                                                                                                                               <  3:406975/149‑1 (MQ=255)
       cTGTTACGACGCACGGCAGATTTGCGATAGCGGAATTCAGAGGCGATTTCGACGTCGCACGGAATACCTGCTAGCGATTCAAACCAGTAGCGGGAAACCATACCGGAGTTATAAGAAGTACCACAGGCGAGGATCTGAATATGCTCAAc                                                                                                                                          <  2:231364/149‑1 (MQ=255)
          ttACGACGCACGGCAGATTTGCGATAGCGGAATTCAGAGGCGATTTCGACGTCGCACGGAATACCTGCTAGCGATTCAAACCAGTAGCGGGAAACCATACCGGAGTTATAAGAAGTACCACAGGCGAGGATCTGAATATGCTCAACCtt                                                                                                                                       >  4:538727/1‑149 (MQ=255)
                      gCAGATTTGCGATAGCGGAATTCAGAGGCGATTTCGACGTCGCACGGAATACCTGCTAGCGATTCAAACCAGTAGCGGGAAACCATACCGGAGTTATAAGAAGTACCACAGGCGAGGATCTGAATATGCTCAACCTTCGACAGCAGTtc                                                                                                                           <  3:315495/149‑1 (MQ=255)
                         gATTTGCGATAGCGGAATTCAGAGGCGATTTCGACGTCGCACGGAATACCTGCTAGCGATTCAAACCAGTAGCGGGAAACCATACCGGAGTTATAAGAAGTACCACAGGCGAGGATCTGAATATGCTCAACCTTCGACAGCAGTtcgtc                                                                                                                        <  3:261567/149‑1 (MQ=255)
                                gATAGCGGAATTCAGAGGCGATTTCGACGTCGCACGGAATACCTGCTAGCGATTCAAACCAGTAGCGGGAAACCATACCGGAGTTATAAGAAGTACCACAGGCGAGGATCTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTc                                                                                                                 >  4:98412/1‑149 (MQ=255)
                                      ggAATTCAGAGGCGATTTCGACGTCGCACGGAATACCTGCTAGCGATTCAAACCAGTAGCGGGAAACCATACCGGAGTTATAAGAAGTACCACAGGCGAGGATCTGAATATGCTCAACCTTCGAGAGCAGTTCGTCGGCGTTCGTTccc                                                                                                           <  1:506775/149‑1 (MQ=255)
                                       gAATTCAGAGGCGATTTCGACGTCGCACGGAATACCTGCTAGCGATTCAAACCAGTAGCGGGAAACCATACCGGAGTTATAAGAAGTACCACAGGCGAGGATCTGAATATGCTCAACCTTCGACAGCAGCTCGTCGGCGTTCGGTCCCa                                                                                                          <  3:463216/149‑1 (MQ=255)
                                          ttCAGAGGCGATTTCGACGTCGCACGGAATACCTGCTAGCGATTCAAACCAGTAGCGGGAAACCATACCGGAGTTATAAGAAGTACCACAGGCGAGGATCTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCt                                                                                                       >  3:418689/1‑149 (MQ=255)
                                             agagGCGATTTCGACGTCGCACGGAATACCTGCTAGCGATTCAAACCAGTAGCGGGAAACCATACCGGAGTTATAAGAAGTACCACAGGCGAGGATCTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGc                                                                                                    <  3:541428/149‑1 (MQ=255)
                                                      ttCGACGTCGCACGGAATACCTGCTAGCGATTCAAACCAGTAGCGGGAAACCATACCGGAGTTATAAGAAGTACCACAGGCGAGGATCTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCaa                                                                                           <  3:13549/149‑1 (MQ=255)
                                                       tCGACGTCGCACGGAATACCTGCTAGCGATTCAAACCAGTAGCGGGAAACCATACCGGAGTTATAAGAAGTACCACAGGCGAGGATCTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAAc                                                                                          <  1:94613/149‑1 (MQ=255)
                                                       tCGACGTCGCACGGAATACCTGCTAGCGATTCAAACCAGTAGCGGGAAACCATACCGGAGTTATAAGAAGTACCACAGGCGAGGATCTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAAc                                                                                          <  4:394881/149‑1 (MQ=255)
                                                         gtcGTCGCACGGAATACCTGCTAGCGATTCAAACCAGTAGCGGTAAACCATACCTGAGTTTTAAGAAGTACCACAGTCGAGGATCTTAATATACTCAACCTTCGACAGCAGTTCTTCGGCGTTCGGTCGCAGCTCTCGTAAATCAACCt                                                                                        >  4:15569/3‑149 (MQ=255)
                                                         gACGTCGCACGGAATACCTGCTAGCGATTCAAACCAGTAGCGGGAAACCATACCGGAGTTATAAGAAGTACCACAGGCGAGGATCTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTaaaacaccct                                                                                        >  4:25312/1‑142 (MQ=255)
                                                         gACGTCGCACGGAATACCTGCTAGCGATTCAAACCAATAGCGGGAAACCATACCGGAGTTATAAGAAGTACCACAGGCGAGGATCTGAATATTCTCAACCTGCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCt                                                                                        >  2:79348/1‑149 (MQ=255)
                                                                cACGGAATACCTGCTAGCGATTCAAACCAGTAGCGGGAAACCATACCGGAGTTATAAGAAGTACCACAGGCGAGGATCTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTg                                                                                 >  3:217378/1‑149 (MQ=255)
                                                                 aCGGAATACCTGCTAGCGATTCAAACCAGTAGCGGGAAACCATACCGGAGTTATAAGAAGTACCACAGGCGAGGATCTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTgg                                                                                >  4:282364/1‑149 (MQ=255)
                                                                 aCGGAATACCTGCTAGCGATTCAAACCAGTAGCGGGAAACCATACCGGAGTTATAAGAAGTACCACAGGCGAGGATCTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTgg                                                                                >  4:556030/1‑149 (MQ=255)
                                                                     aaTACCTGCTAGCGATTCAAACCAGTAGCGGGAAACCATACCGGAGTTATAAGAAGTACCACAGGCGAGGATCTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGa                                                                            <  3:98412/149‑1 (MQ=255)
                                                                      aTACCTGCTAGCGATTCAAACCAGTAGCGGGAAACCATACCGGAGTTATAAGAAGTACCACAGGCGAGGATCTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGAt                                                                           >  1:148537/1‑149 (MQ=255)
                                                                      aTACCTGCTAGCGATTCAAACCAGTAGCGGGAAACCATACCGGAGTTATAAGAAGTACCACAGGCGAGGATCTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGAt                                                                           <  2:205358/149‑1 (MQ=255)
                                                                        aCCTGCTAGCGATTCAAACCAGTAGCGGGAAACCATACCGGAGTTATAAGAAGTACCACAGGCGAGGATCTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGa                                                                                      <  2:42652/136‑1 (MQ=255)
                                                                        aCCTGCTAGCGATTCAAACCAGTAGCGGGAAACCATACCGGAGTTATAAGAAGTACCACAGGCGAGGATCTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGa                                                                                      >  1:42652/1‑136 (MQ=255)
                                                                        aCCTGCTAGCGATTCAAACCAGTAGCGGGAAACCATACCGGAGTTATAAGAAGTACCACAGGCGAGGATCTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGc                                                                         <  1:118577/149‑1 (MQ=255)
                                                                                  gATTCAAACCAGTAGCGGGAAACCATACCGGAGTTATAAGAAGTACCACAGGCGAGGATCTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAg                                                               >  3:403156/1‑149 (MQ=255)
                                                                                    ttCGAACCAGTAGCGGGAAACCATACCGGAGTTATAAGAAGTACCACAGGCGAGGATCTGGATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGATCGTGGCTGATGCGTCCGGTAAggg                                                             >  1:505699/1‑149 (MQ=255)
                                                                                    ttCAAACCAGTTGCGGGAAACCATACCGGAGTTATAAGAAGTACCACAGGCGAGGATCTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAg                                                                                                         >  3:378082/1‑105 (MQ=255)
                                                                                    ttCAAACCAGTAGCGGGAAATCATACCGGAGTTATAAGAAGTACCACAGGCGAGGATCTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAggg                                                             >  3:127785/1‑149 (MQ=255)
                                                                                    ttCAAACCAGTAGCGGGAAACCATACCGGAGTTATAAGAAGTACCACAGGCGAGGATCTGGATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAggg                                                             >  3:191488/1‑149 (MQ=255)
                                                                                    ttCAAACCAGTAGCGGGAAACCATACCGGAGTTATAAGAAGTACCACAGGCGAGGATCTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAg                                                                                                         <  4:378082/105‑1 (MQ=255)
                                                                                    ttCAAACCAGTAGCGGGAAACCATACCGGAGTTATAAGAAGTACCACAGGCGAGGATCTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAggg                                                             >  2:238642/1‑149 (MQ=255)
                                                                                       aaaCCAGTAGCGGGAAACCATACCGGAGTTATAAGAAGTACCACAGGCGAGGATCTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGgtgt                                                          >  4:413562/1‑149 (MQ=255)
                                                                                           cAGTAGCGGGAAACCATACCGGAGTTATAAGAAGTACCACAGGCGAGGATCTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTCAGGGTGtgttt                                                      >  3:341004/1‑149 (MQ=255)
                                                                                              tAGCGGGAAACCATACCGGAGTTATAAGAAGTACCACAGGCGAGGATCTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGAt                                                   <  3:192462/149‑1 (MQ=255)
                                                                                               aGCGGGAAACCATACCGGAGTTATAAGAAGTACCACAGGCGAGGATCTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATc                                                  >  1:211589/1‑149 (MQ=255)
                                                                                                  gggAAACCATACCGGAGTTATAAGAAGTACCACAGGCGAGGATCTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATcgcg                                               >  4:390401/1‑149 (MQ=255)
                                                                                                   ggAAACCAAACCGGAGTTATAAGATGTACCTCAGGCGAGGATCTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGt                                              <  2:387694/149‑1 (MQ=255)
                                                                                                      aaCCATACCGGAGTTATAAGAAGTACCACAGGCGAGGATCTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGtt                                                                                                                  >  4:585388/1‑78 (MQ=255)
                                                                                                      aaCCATACCGGAGTTATAAGAAGTACCACAGGCGAGGATCTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGtt                                                                                                                  <  3:585388/78‑1 (MQ=255)
                                                                                                      aaCCATACCGGAGTTATAAGAAGTACCACAGGCGAGGATCTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCg                                                                    >  1:120834/1‑124 (MQ=255)
                                                                                                      aaCCATACCGGAGTTATAAGAAGTACCACAGGCGAGGATCTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCg                                                                    <  2:120834/124‑1 (MQ=255)
                                                                                                        ccATACCGGAGTTATAAGAAGTACCACAGGCGAGGATCTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGc                                         >  3:339034/1‑149 (MQ=255)
                                                                                                             ccGGAGTTATAAGAAGTACCACAGGCGAGGATCTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTc                                    >  3:398661/1‑149 (MQ=255)
                                                                                                                gAGTTATAAGAAGTACCACAGGCGAGGATCTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTa                                 <  3:282364/149‑1 (MQ=255)
                                                                                                                  gTTATAAGAAGTACGACAGGCGAGGATCTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGa                               <  4:339845/149‑1 (MQ=255)
                                                                                                                      taAGAAGTACCACAGGCGAGGATCTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGc                                                                         >  3:376450/1‑103 (MQ=255)
                                                                                                                      taAGAAGTACCACAGGCGAGGATCTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGc                                                                         <  4:376450/103‑1 (MQ=255)
                                                                                                                       aagaagTACCACAGGCGAGGATCTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATctct                          <  1:79348/149‑1 (MQ=255)
                                                                                                                        agaagTACCACAGGCGAGGATCTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCtt                         <  1:276146/149‑1 (MQ=255)
                                                                                                                         gaagTACCACAGGCGAGGATCTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGa                                                    >  4:429887/1‑121 (MQ=255)
                                                                                                                         gaagTACCACAGGCGAGGATCTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGa                                                    <  3:429887/121‑1 (MQ=255)
                                                                                                                          aagTACCACAGGCGAGGATCTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTc                       <  3:243550/149‑1 (MQ=255)
                                                                                                                           agTACCACAGGCGAGGATCTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCt                      <  4:295899/149‑1 (MQ=255)
                                                                                                                             ggccccaGGCGGGCCTCTGCATATGCTCAACCTTCGTCAGCAGTTCGTCGCCGTTCGTTCACAGCTCGGTTAAAACAACCCGTCCGTGGCTGATGCGTCCGGTTAGGGTGTTTTTGATCGCGTGCGGCTGTTCGTAGATCTCTTTCTGc                    <  1:320963/144‑1 (MQ=255)
                                                                                                                                   aGGCGAGGATCTGGATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGATCGTGGCTGATGCGTCCGGTAAGGGCGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTaa              <  2:505699/149‑2 (MQ=255)
                                                                                                                                   aGGCGAGGATCTGGATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAg              <  4:191488/149‑1 (MQ=255)
                                                                                                                                       gAGGATCTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGAc          <  1:381575/149‑1 (MQ=255)
                                                                                                                                         ggATCTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACgg        >  3:311129/1‑149 (MQ=255)
                                                                                                                                               gAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATg  >  3:154520/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
CATCAGGCTGTTACGACGCACGGCAGATTTGCGATAGCGGAATTCAGAGGCGATTTCGACGTCGCACGGAATACCTGCTAGCGATTCAAACCAGTAGCGGGAAACCATACCGGAGTTATAAGAAGTACCACAGGCGAGGATCTGAATATGCTCAACCTTCGACAGCAGTTCGTCGGCGTTCGGTCCCAGCTCGCTTAAATCAACCTGACCGTGGCTGATGCGTCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATG  >  NC_000913/3912637‑3912928

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑