Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,938,255 T→A 9.5% L10Q (CTG→CAG)  rbsR → transcriptional repressor of ribose metabolism

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,938,2550TA 9.5% 238.2 / 14.5 74L10Q (CTG→CAG) rbsRtranscriptional repressor of ribose metabolism
Reads supporting (aligned to +/- strand):  ref base T (37/30);  new base A (4/3);  total (41/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.66e-01

CCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGCTCTGGCGCGTAGCCTCAAACTCAATC  >  NC_000913/3938107‑3938398
                                                                                                                                                    |                                                                                                                                               
ccAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCt                                                                                                                                                 <  4:317082/149‑1 (MQ=255)
          ttCGTTTTGCCCATGCTGCCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGtt                                                                                                                                       >  1:392023/1‑149 (MQ=255)
          ttCGTTTTGCCCATGCTGCCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGCCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGtt                                                                                                                                       >  1:593037/1‑149 (MQ=255)
           tCGTTTTGCCCATGCTGCCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGttt                                                                                                                                      <  3:13079/149‑1 (MQ=255)
               tttGCCCATGCTGCCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTAc                                                                                                                                  <  3:329139/149‑1 (MQ=255)
                    ccATGCTGCCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCaa                                                                                                                             >  1:155837/1‑149 (MQ=255)
                     cATGCTGCCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAAc                                                                                                                            >  4:31930/1‑149 (MQ=255)
                     cATGCTGCCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAAc                                                                                                                            >  2:153473/1‑149 (MQ=255)
                          tgcCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGttt                                                                                                                       <  4:133882/149‑1 (MQ=255)
                              gCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCa                                                                                                                   >  2:584779/1‑149 (MQ=255)
                                  cGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGtt                                                                                                               <  3:294311/149‑1 (MQ=255)
                                    aTTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTAt                                                                                                             <  3:534771/149‑1 (MQ=255)
                                       gCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCaa                                                                                                          <  2:224001/149‑1 (MQ=255)
                                         cGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATa                                                                                                        <  4:547676/149‑1 (MQ=255)
                                          gTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATaa                                                                                                       >  3:579772/1‑149 (MQ=255)
                                           tAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATaaa                                                                                                      >  2:505746/1‑149 (MQ=255)
                                           tAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATaaa                                                                                                      <  1:269571/149‑1 (MQ=255)
                                              cacGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGAt                                                                                                   <  2:182864/149‑1 (MQ=255)
                                                 gTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATa                                                                                                        <  1:488406/141‑1 (MQ=255)
                                                 gTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATa                                                                                                        >  2:488406/1‑141 (MQ=255)
                                                     aGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCg                                                                                            >  3:458182/1‑149 (MQ=255)
                                                           acaACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTg                                                                                      >  3:574271/1‑149 (MQ=255)
                                                                 ttCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGa                                                                                >  3:500614/1‑149 (MQ=255)
                                                                                 gAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGa                                                                >  2:129449/1‑149 (MQ=255)
                                                                                  aaGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGaa                                                               <  1:153473/149‑1 (MQ=255)
                                                                                   agagATCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAg                                                              >  1:401474/1‑149 (MQ=255)
                                                                                     agaTCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGtt                                                                                                               >  3:157645/1‑98 (MQ=255)
                                                                                     agaTCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGtt                                                                                                               <  4:157645/98‑1 (MQ=255)
                                                                                     agaTCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAgcg                                                            >  3:62810/1‑149 (MQ=255)
                                                                                       aTCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAgcggc                                                          <  3:494473/149‑1 (MQ=255)
                                                                                            cGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTa                                                     >  3:133817/1‑149 (MQ=255)
                                                                                              cATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTaaa                                                   <  4:500614/149‑1 (MQ=255)
                                                                                              cATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTaaa                                                   <  2:216848/149‑1 (MQ=255)
                                                                                              cATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTaaa                                                   <  4:574271/149‑1 (MQ=255)
                                                                                               aTTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTTGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAg                                                  <  3:1624/149‑1 (MQ=255)
                                                                                               aTTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAAc                                                                                                                            >  2:533631/1‑75 (MQ=255)
                                                                                               aTTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAAc                                                                                                                            <  1:533631/75‑1 (MQ=255)
                                                                                               aTTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAgcggc                                                          >  2:103053/1‑141 (MQ=255)
                                                                                               aTTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAgcggc                                                          <  1:103053/141‑1 (MQ=255)
                                                                                                       aCAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAAt                                          <  4:456911/149‑1 (MQ=255)
                                                                                                         aGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTa                                        >  3:365327/1‑149 (MQ=255)
                                                                                                          ggCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTAc                                       >  1:281732/1‑149 (MQ=255)
                                                                                                           gCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTAct                                      >  1:527346/1‑148 (MQ=255)
                                                                                                              gagGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACgcgc                                   >  3:223611/1‑149 (MQ=255)
                                                                                                               agGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGcc                                  >  4:277365/1‑149 (MQ=255)
                                                                                                                ggTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCa                                 >  1:346904/1‑149 (MQ=255)
                                                                                                                 gTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCAt                                >  2:574165/1‑149 (MQ=255)
                                                                                                                 gTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCAt                                <  4:458182/149‑1 (MQ=255)
                                                                                                                   gACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCATCa                              <  4:579772/149‑1 (MQ=255)
                                                                                                                           gCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGCTCTGgc                      <  2:401474/149‑1 (MQ=255)
                                                                                                                             tACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGCTCTGgcgc                    >  1:447087/1‑149 (MQ=255)
                                                                                                                                aaTGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTGCCTCAACAGGTTCTCACGTTATCAATAAAGATc                                                                                                  <  2:523594/68‑1 (MQ=255)
                                                                                                                                aaTGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATc                                                                                                  >  1:523594/1‑68 (MQ=255)
                                                                                                                                aaTGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAATTTGAAGCGGCGATTata                                                   <  4:423652/115‑3 (MQ=255)
                                                                                                                                aaTGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTaaa                                                   >  3:423652/1‑115 (MQ=255)
                                                                                                                                aaTGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGCTCTGGCGCGTa                 >  2:249625/1‑149 (MQ=255)
                                                                                                                                  tGAAAGATGTTACCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGCTCTGGCGCGTAGc               >  3:469604/1‑149 (MQ=255)
                                                                                                                                   gAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGCTCTGGCGCGTAGcc              <  3:121973/149‑1 (MQ=255)
                                                                                                                                    aaaGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGCTCTGGCGCGTAGCCt             >  2:532601/1‑149 (MQ=255)
                                                                                                                                      aGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCaa                                           <  1:186667/117‑1 (MQ=255)
                                                                                                                                      aGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCaa                                           >  2:186667/1‑117 (MQ=255)
                                                                                                                                      aGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGCTCTGgc                      <  1:167711/138‑1 (MQ=255)
                                                                                                                                      aGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGCTCTGgc                      >  2:167711/1‑138 (MQ=255)
                                                                                                                                       agaaacgCCCGCCAGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGCTCTGGCGCGTAGCCTCaa          >  3:121336/7‑149 (MQ=255)
                                                                                                                                        aTGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGATTCGACAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGCTCTGGCGCGTAGCCTCaaa         >  1:456840/1‑149 (MQ=255)
                                                                                                                                          aacgCCCGCCAGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAAc                                               >  1:281207/4‑109 (MQ=255)
                                                                                                                                          aacgCCCGCCAGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAAc                                               <  2:281207/106‑1 (MQ=255)
                                                                                                                                          aacgCCCGCCAGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGCTCTGGCGCGTAGCCTCAAACt       >  1:338378/4‑149 (MQ=255)
                                                                                                                                           ttGCCCGCCTGGCGAGCGTTTGTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGCTTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGCTCTGGCGCGTAGCCTCAAACtc      <  3:362337/149‑1 (MQ=255)
                                                                                                                                           acgCCCGCCAGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAg                                                                                                     <  4:557529/52‑1 (MQ=255)
                                                                                                                                           acgCCCGCCAGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAg                                                                                                     >  3:557529/3‑54 (MQ=255)
                                                                                                                                           acgCCCGCCAGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGCTCTGGCGCGTAGCCTCAAACtc      <  4:121336/147‑1 (MQ=255)
                                                                                                                                             gCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGCTCTGGCGCGTAGCCTCAAACtcaa    <  3:217271/149‑1 (MQ=255)
                                                                                                                                               ccGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGCTCTGGCGCGTAGCCTCAAACtcaatc  >  2:51556/1‑149 (MQ=255)
                                                                                                                                               ccGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGCTCTGGCGCGTAGCCTCAAACtcaatc  >  3:429104/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
CCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGCTCTGGCGCGTAGCCTCAAACTCAATC  >  NC_000913/3938107‑3938398

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: