Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,117,027	C→A	100%	A66S (GCG→TCG)	glpK ←	glycerol kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,117,027	0	C	A	100.0%	217.1 / NA	59	A66S (GCG→TCG)	glpK	glycerol kinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (29/30);  total (29/30)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACG  >  NC_000913/4116882‑4117163                                                                                                                                                  |                                                                                                                                         tCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAg                                                                                                                                       >  3:583253/1‑149 (MQ=255)   gCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCa                                                                                                                                     >  1:300556/1‑149 (MQ=255)   gCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCa                                                                                                                                     >  3:70255/1‑149 (MQ=255)   gCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCa                                                                                                                                     >  4:344152/1‑149 (MQ=255)        tttCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCt                                                                                                                                <  1:440689/149‑1 (MQ=255)            tGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCa                                                                                                                            <  3:1081/149‑1 (MQ=255)                gTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGt                                                                                                                        <  3:216865/149‑1 (MQ=255)                 tACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTg                                                                                                                       <  3:441541/149‑1 (MQ=255)                          aCTGCCAGACAATGGCGTTATAGATTGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTgg                                                                                                              <  1:14544/149‑1 (MQ=255)                          aCTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGTGTGGAGCTTTgg                                                                                                              <  3:42936/149‑1 (MQ=255)                                    aaTGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGa                                                                                                    <  4:229755/149‑1 (MQ=255)                                           ttATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCAt                                                                                             <  3:17085/149‑1 (MQ=255)                                           ttATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCAt                                                                                             >  4:576096/1‑149 (MQ=255)                                            tataGATAGGCTTGCCGGTTTCTCTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCAtt                                                                                            <  3:579824/149‑1 (MQ=255)                                              tagatagGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTgg                                                                                          <  3:344152/149‑1 (MQ=255)                                                 atagGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTc                                                                                       >  2:189944/1‑149 (MQ=255)                                                   agGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgt                                                                                     >  4:487344/1‑149 (MQ=255)                                                     gCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgtgt                                                                                   >  1:332720/1‑149 (MQ=255)                                                     gCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgtgt                                                                                   >  4:315136/1‑149 (MQ=255)                                                       ttGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTATTACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTc                                                                                 >  3:309625/1‑149 (MQ=255)                                                          ccGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTAc                                                                              >  4:293107/1‑149 (MQ=255)                                                              tttCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCaa                                                                          >  3:549310/1‑149 (MQ=255)                                                                  tttttCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTg                                                                      >  1:42172/1‑149 (MQ=255)                                                                       cccAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGtttt                                                                 <  4:159551/149‑1 (MQ=255)                                                                       cccAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATGTCCATTGGGTCGTGTTCTACCCAACCTGGtttt                                                                 <  3:155160/149‑1 (MQ=255)                                                                         cAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTgg                                                               <  3:236780/149‑1 (MQ=255)                                                                         cAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTgg                                                               >  2:212082/1‑149 (MQ=255)                                                                           gACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGt                                                             <  4:309625/149‑1 (MQ=255)                                                                            aCAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTTGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTa                                                            >  4:341879/1‑149 (MQ=255)                                                                            aCAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATAGCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTa                                                            <  2:79167/149‑1 (MQ=255)                                                                             cAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAg                                                           <  2:300556/149‑1 (MQ=255)                                                                                  gTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGt                                                                                                                        >  4:146534/1‑83 (MQ=255)                                                                                  gTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGt                                                                                                                        <  3:146534/83‑1 (MQ=255)                                                                                   tGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGc                                                     <  1:372574/149‑1 (MQ=255)                                                                                         cACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAAt                                               <  2:378959/149‑1 (MQ=255)                                                                                          aCGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAAtt                                              >  2:247964/1‑149 (MQ=255)                                                                                                 ttCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTgg                                                               <  3:409787/125‑1 (MQ=255)                                                                                                 ttCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTgg                                                               >  4:409787/1‑125 (MQ=255)                                                                                                 ttCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTg                                       >  4:315618/1‑149 (MQ=255)                                                                                                  tCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGc                                      <  4:427246/149‑1 (MQ=255)                                                                                                      aaTACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAAt                                               <  3:298129/136‑1 (MQ=255)                                                                                                      aaTACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAAt                                               >  4:298129/1‑136 (MQ=255)                                                                                                          ccGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCt                              >  2:135950/1‑149 (MQ=255)                                                                                                           cGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCAt                                                                                             <  4:59735/85‑1 (MQ=255)                                                                                                           cGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCAt                                                                                             >  3:59735/1‑85 (MQ=255)                                                                                                                gCTGCAATTTGATCGGAACTGATATCGGCTTTTGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGat                        <  3:341879/149‑1 (MQ=255)                                                                                                                gCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGat                        <  3:307834/149‑1 (MQ=255)                                                                                                                  tGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTgg                                                                                                              >  4:269959/1‑61 (MQ=255)                                                                                                                  tGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTgg                                                                                                              <  3:269959/61‑1 (MQ=255)                                                                                                                  tGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGatat                      >  2:237748/1‑149 (MQ=255)                                                                                                                  tGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGatat                      >  1:589788/1‑149 (MQ=255)                                                                                                                    cAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTg                    <  3:97161/149‑1 (MQ=255)                                                                                                                    cAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTg                    <  4:583253/149‑1 (MQ=255)                                                                                                                       tttGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCa                 >  3:17179/1‑149 (MQ=255)                                                                                                                       tttGATCGGAACTGATATCGGCTTTAGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCa                 >  4:516203/1‑149 (MQ=255)                                                                                                                            tCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTg            >  2:83091/1‑149 (MQ=255)                                                                                                                             cGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa           <  4:17179/149‑1 (MQ=255)                                                                                                                             cGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa           <  3:315136/149‑1 (MQ=255)                                                                                                                                      tatCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACg  >  1:507623/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                         TCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACG  >  NC_000913/4116882‑4117163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑