Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,381,389 G→T 5.9% G310G (GGC→GGA)  frdA ← anaerobic fumarate reductase catalytic and NAD/flavoprotein subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,381,3890GT 5.9% 234.9 / 4.4 68G310G (GGC→GGA) frdAanaerobic fumarate reductase catalytic and NAD/flavoprotein subunit
Reads supporting (aligned to +/- strand):  ref base G (32/32);  new base T (2/2);  total (34/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.14e-01

CATGGTGTAGTGTGCGGTCGGACGTACCGGAATCGGTTCTTTAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCA  >  NC_000913/4381242‑4381528
                                                                                                                                                   |                                                                                                                                           
cATTGTGTAGTGTGCGGTCGGACGTACCGGAATCGGTTCTTTAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGc                                                                                                                                            >  4:499157/1‑149 (MQ=255)
cATGGTGTAGTGTGCGGTCGGACGTACCGGAATCGGTTCTTTAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGc                                                                                                                                            >  1:369398/1‑149 (MQ=255)
         gtgtGCGGTCGGACGTACCGGAATCGGTTCTTTAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACgcggcg                                                                                                                                   >  3:196073/1‑149 (MQ=255)
              cGGTCGGACGTACCGGAATCGGTTCTTTAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGgag                                                                                                                              >  2:327498/1‑149 (MQ=255)
                gTCGGACGTACCGGAATCGGTTCTTTAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGAt                                                                                                                            >  4:381523/1‑149 (MQ=255)
                      cGTACCGGAATCGGTTCTTTAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACGTCGCCACGCGGCGTGGAGATGGTGtt                                                                                                                      <  2:455103/149‑1 (MQ=255)
                       gTACCGGAATCGGTTCTTTAGCCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTg                                                                                                                     >  3:204206/1‑149 (MQ=255)
                        tACCGGAATCGGTTCTTTAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGc                                                                                                                    <  3:346139/149‑1 (MQ=255)
                          ccGGAATCGGTTCTTTAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCt                                                                                                                  <  4:33297/149‑1 (MQ=255)
                           cGGAATCGGTTCTTTAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCtt                                                                                                                 <  2:16383/149‑1 (MQ=255)
                              aaTCGGTTCTTTAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTAc                                                                                                              <  4:543473/149‑1 (MQ=255)
                              aaTCGGTTCTTTAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCACAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTAc                                                                                                              <  4:117018/149‑1 (MQ=255)
                                tCGGTTCTTTAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCTCCACGCCGCGTGGCGAt                                                                                                                            >  1:444720/1‑133 (MQ=255)
                                tCGGTTCTTTAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCTCCACGCCGCGTGGCGAt                                                                                                                            <  2:444720/133‑1 (MQ=255)
                                tCGGTTCTTTAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGTCTTTACGc                                                                                                            >  2:486490/1‑149 (MQ=255)
                                tCGGTTCTTTAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGc                                                                                                            >  4:1445/1‑149 (MQ=255)
                                tCGGTTCTTTAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGc                                                                                                            >  1:135438/1‑149 (MQ=255)
                                   gTTCTTTAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCAt                                                                                                         >  2:542204/1‑149 (MQ=255)
                                   gTTCTTTAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCAt                                                                                                         >  1:27768/1‑149 (MQ=255)
                                    ttCTTTAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCAtt                                                                                                        >  1:121488/1‑149 (MQ=255)
                                     tCTTTAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTc                                                                                                       <  1:488679/149‑1 (MQ=255)
                                       tttAGCCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGt                                                                                                     <  4:204206/149‑1 (MQ=255)
                                       tttAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCTCCACGCCGCGTGGCGAt                                                                                                                            >  4:384869/1‑126 (MQ=255)
                                       tttAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCTCCACGCCGCGTGGCGAt                                                                                                                            <  3:384869/126‑1 (MQ=255)
                                       tttAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGt                                                                                                     <  4:34325/149‑1 (MQ=255)
                                               gATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAg                                                                                             <  3:183749/149‑1 (MQ=255)
                                                 tCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGc                                                                                           <  3:117589/149‑1 (MQ=255)
                                                   gACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGTCGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAAACACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCt                                                                                         <  1:486490/149‑1 (MQ=255)
                                                     cGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTga                                                                                       <  2:135438/149‑1 (MQ=255)
                                                      gCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTgag                                                                                      >  3:350960/1‑149 (MQ=255)
                                                            gTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACtt                                                                                >  4:1986/1‑149 (MQ=255)
                                                             tACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACttt                                                                               >  4:164508/1‑149 (MQ=255)
                                                                 cTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTcg                                                                           >  1:50191/1‑149 (MQ=255)
                                                                     cGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTg                                                                       <  4:350960/149‑1 (MQ=255)
                                                                      gCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGGGACTTTGTCGCGTgg                                                                      >  2:204432/1‑149 (MQ=255)
                                                                       ccAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGa                                                                     >  4:434789/1‑149 (MQ=255)
                                                                        cAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGAc                                                                    >  4:377085/1‑149 (MQ=255)
                                                                         aGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGAcc                                                                   <  2:199420/149‑1 (MQ=255)
                                                                           ttCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCa                                                                 >  1:263415/1‑149 (MQ=255)
                                                                             cGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGt                                                               >  2:206114/1‑149 (MQ=255)
                                                                               cAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTc                                                             <  1:254060/149‑1 (MQ=255)
                                                                                  aTGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCat                                                          <  3:499157/149‑1 (MQ=255)
                                                                                   tGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCata                                                         <  1:542204/149‑1 (MQ=255)
                                                                                   tGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCata                                                         <  2:568138/149‑1 (MQ=255)
                                                                                    gAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCatat                                                        >  1:133048/1‑149 (MQ=255)
                                                                                         gCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTg                                                   <  1:230176/149‑1 (MQ=255)
                                                                                            gACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGttt                                                <  1:327498/149‑1 (MQ=255)
                                                                                               gTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTc                                             >  1:373050/1‑149 (MQ=255)
                                                                                               gTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTc                                             >  2:400370/1‑149 (MQ=255)
                                                                                                    tGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTc                                        <  1:377689/149‑1 (MQ=255)
                                                                                                     gCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACttt                                                                               >  4:106999/1‑109 (MQ=255)
                                                                                                     gCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACttt                                                                               <  3:106999/109‑1 (MQ=255)
                                                                                                      cAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGc                                      >  1:407283/1‑149 (MQ=255)
                                                                                                      cAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGc                                      >  2:305596/1‑149 (MQ=255)
                                                                                                           ttttCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGc                                 <  3:434789/149‑1 (MQ=255)
                                                                                                            tttCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCg                                <  3:381523/149‑1 (MQ=255)
                                                                                                            tttCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGCGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCg                                <  2:168454/149‑1 (MQ=255)
                                                                                                                     gAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCg                       >  4:418080/1‑149 (MQ=255)
                                                                                                                      aGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCgg                      <  2:133048/149‑1 (MQ=255)
                                                                                                                      aGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCgg                      <  3:1986/149‑1 (MQ=255)
                                                                                                                      aGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCgg                      <  2:333473/149‑1 (MQ=255)
                                                                                                                          taCGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGccc                  <  3:216526/148‑1 (MQ=255)
                                                                                                                           aCGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCa                 >  3:192958/1‑149 (MQ=255)
                                                                                                                             gCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATg               >  1:60027/1‑149 (MQ=255)
                                                                                                                                 gTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGt           <  3:418080/149‑1 (MQ=255)
                                                                                                                                       aTAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCtt     >  4:162557/1‑149 (MQ=255)
                                                                                                                                         aaaCCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGc   <  2:369398/149‑1 (MQ=255)
                                                                                                                                          aaCCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCa  >  1:248518/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                           
CATGGTGTAGTGTGCGGTCGGACGTACCGGAATCGGTTCTTTAACCGGATCGACGCCAACGTACGCTTTCGCCAGTTCGCAGATGAACGGCAGACGTTCATGCAGTTTTTTCTCGCCGAGGTGACGCAAGTCGAGATAAACCACATCGCCACGCGGCGTGGAGATGGTGTTGCCTTTACGCCATTCGTGCCAGAAGGCCTGAGAGACTTTGTCGCGTGGACCCAGTTCCATATATTTGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCA  >  NC_000913/4381242‑4381528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑