Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,551,422 T→A 8.5% intergenic (‑126/‑214) gntP ← / → uxuA fructuronate transporter/mannonate hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,551,4220TA 8.5% 389.7 / 21.5 117intergenic (‑126/‑214)gntP/uxuAfructuronate transporter/mannonate hydrolase
Reads supporting (aligned to +/- strand):  ref base T (53/54);  new base A (5/5);  total (58/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.94e-01

Only 100 of 122 total aligned reads displayed.

AGAGAATGTTAAGCACATGCATAACGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTCATCCGGATA  >  NC_000913/4551275‑4551570
                                                                                                                                                   |                                                                                                                                                    
agagAATGTTAAGCACATGCATAACGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAAtt                                                                                                                                                     >  4:6534/1‑149 (MQ=255)
agagAATGTTAAGCACATGCATAACGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAAtt                                                                                                                                                     >  1:91480/1‑149 (MQ=255)
 gagaATGTTAAGCACATGCATAACGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAAttt                                                                                                                                                    >  2:334415/1‑149 (MQ=255)
   caaTGTTAAGCACATGCATAACGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTgc                                                                                                                                                  <  4:376521/148‑1 (MQ=255)
    aaTGTTAAGCACATGCATAACGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTgca                                                                                                                                                 <  1:246123/149‑1 (MQ=255)
      tGTTAAGCACATGCATAACGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTgcagc                                                                                                                                               >  1:108172/1‑149 (MQ=255)
      tGTTAAGCACATGCATAACGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAAATTgcagc                                                                                                                                               >  1:125419/1‑149 (MQ=255)
         tAAGCACATGCATAACGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGcaac                                                                                                                                            <  2:327185/149‑1 (MQ=255)
         tAAGCACATGCATAACGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAAATTGCAGcaaa                                                                                                                                            <  2:194975/149‑2 (MQ=255)
          aaGCACATGCATAACGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGcaaca                                                                                                                                           >  4:294441/1‑149 (MQ=255)
          aaGCACATGCATAACGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAAATTGCAGcaaag                                                                                                                                           <  2:125419/149‑3 (MQ=255)
            gCACATGCATAACGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCaacaac                                                                                                                                         <  2:209662/149‑1 (MQ=255)
               caTGCATAACGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCAt                                                                                                                                      <  1:43795/149‑1 (MQ=255)
                 tGCATAACGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATga                                                                                                                                    <  1:189553/149‑1 (MQ=255)
                   cATAACGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATgaga                                                                                                                                  <  1:79016/149‑1 (MQ=255)
                     tAACGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGt                                                                                                                                >  1:564683/1‑149 (MQ=255)
                     tAACGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGt                                                                                                                                <  4:337559/149‑1 (MQ=255)
                         gTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGaaa                                                                                                                            <  3:294441/149‑1 (MQ=255)
                         gTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGaaa                                                                                                                            >  3:143493/1‑149 (MQ=255)
                         gTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAAATTGCAGcaaa                                                                                                                                            <  3:238945/133‑2 (MQ=255)
                          ttttACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCTTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAAt                                                                                                                           >  4:45126/1‑149 (MQ=255)
                          ttttACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTATGCTGCAATTTGCAGCAACAACCATGAGAGTGAAAt                                                                                                                           >  4:395731/1‑149 (MQ=255)
                             taccttacctGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCt                                                                                                                        >  3:443866/1‑149 (MQ=255)
                                 ttacctGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTtgtg                                                                                                                    >  1:598273/1‑149 (MQ=255)
                                 ttacctGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTtgtg                                                                                                                    >  3:112674/1‑149 (MQ=255)
                                    cctGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGAtg                                                                                                                 <  3:346760/149‑1 (MQ=255)
                                     ctGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACACCCATGAGAGTGAAATTCTTGTGAtgt                                                                                                                >  3:24692/1‑149 (MQ=255)
                                     ctGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCtt                                                                                                                       >  4:294396/1‑142 (MQ=255)
                                     ctGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCtt                                                                                                                       <  3:294396/142‑1 (MQ=255)
                                     ctGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGAtgt                                                                                                                <  1:334415/149‑1 (MQ=255)
                                      tGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGcaac                                                                                                                                            >  3:449971/1‑120 (MQ=255)
                                      tGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGcaac                                                                                                                                            <  4:449971/120‑1 (MQ=255)
                                           gAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAGCCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTaa                                                                                                          <  4:243875/149‑1 (MQ=255)
                                                gttgttATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAAATTGCAGcaaa                                                                                                                                            >  3:296931/1‑109 (MQ=255)
                                                gttgttATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAAATTGCAGcaaa                                                                                                                                            <  4:296931/110‑2 (MQ=255)
                                                   gttATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTc                                                                                                  >  1:497711/1‑149 (MQ=255)
                                                           gggCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAAc                                                                                                         <  1:50148/134‑1 (MQ=255)
                                                               gATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGtt                                                                                      >  4:241001/1‑149 (MQ=255)
                                                               gATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGtt                                                                                      <  3:395731/149‑1 (MQ=255)
                                                                 tatgttatgtAAATTGGTCAACCATTGTTGCGATTAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGGTGa                                                                                    <  4:380384/149‑1 (MQ=255)
                                                                  atgttatgtAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGAc                                                                                   <  3:208523/149‑1 (MQ=255)
                                                                    gttatgtAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACAt                                                                                 >  3:451313/1‑149 (MQ=255)
                                                                      tatgtAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGt                                                                               <  4:24692/149‑1 (MQ=255)
                                                                       atgtAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTc                                                                              >  2:296022/1‑149 (MQ=255)
                                                                       atgtAAATTGGGCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTc                                                                              >  1:443787/1‑149 (MQ=255)
                                                                          tAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCACTTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGTATTCGGGTTGACATGTCTTa                                                                           >  2:377248/1‑149 (MQ=255)
                                                                           aaaTTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTAc                                                                          <  4:487873/149‑1 (MQ=255)
                                                                             aTTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCa                                                                        >  3:421671/1‑149 (MQ=255)
                                                                                ggTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGTAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCaaaa                                                                     <  3:398290/149‑1 (MQ=255)
                                                                                 gTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAg                                                                    <  2:564683/149‑1 (MQ=255)
                                                                                  tCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAgg                                                                   >  1:498915/1‑149 (MQ=255)
                                                                                    aaCCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGaa                                                                                              >  3:357037/1‑120 (MQ=255)
                                                                                    aaCCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTa                                                                 >  3:224707/1‑149 (MQ=255)
                                                                                     aCCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGtt                                                                                      >  3:491338/1‑127 (MQ=255)
                                                                                     aCCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGtt                                                                                      <  4:491338/127‑1 (MQ=255)
                                                                                     aCCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAg                                                                <  4:203486/149‑1 (MQ=255)
                                                                                     aCCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAg                                                                >  3:138161/1‑149 (MQ=255)
                                                                                       cATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGaa                                                              <  3:300154/149‑1 (MQ=255)
                                                                                        aTTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAAc                                                             <  3:6534/149‑1 (MQ=255)
                                                                                            ttgCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATga                                                                                                                                    >  3:117742/1‑74 (MQ=255)
                                                                                            ttgCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATga                                                                                                                                    <  4:117742/74‑1 (MQ=255)
                                                                                            ttgCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTtat                                                         >  1:367052/1‑149 (MQ=255)
                                                                                              gCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATAc                                                       >  4:543247/1‑149 (MQ=255)
                                                                                              gCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATAc                                                       <  2:459218/149‑1 (MQ=255)
                                                                                                gATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGc                                                     >  2:394059/1‑149 (MQ=255)
                                                                                                gATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGc                                                     >  1:326880/1‑149 (MQ=255)
                                                                                                gATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGc                                                     >  1:432874/1‑149 (MQ=255)
                                                                                                gATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGc                                                     >  3:127608/1‑149 (MQ=255)
                                                                                                 aTGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGcc                                                    >  1:58609/1‑149 (MQ=255)
                                                                                                         cacaTCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATCCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATc                                            <  3:513815/149‑1 (MQ=255)
                                                                                                          acaTCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATcc                                           <  2:294763/149‑1 (MQ=255)
                                                                                                            aTCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGa                                         >  4:408952/1‑149 (MQ=255)
                                                                                                             tCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGTTAGAACTTATACGCCATCTCATCCGAt                                        <  3:391128/149‑1 (MQ=255)
                                                                                                             tCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGAt                                        <  2:58609/149‑1 (MQ=255)
                                                                                                                   gATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACg                                  >  4:332977/1‑149 (MQ=255)
                                                                                                                   gATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACg                                  >  2:163896/1‑149 (MQ=255)
                                                                                                                     tCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGcc                                >  2:278437/1‑149 (MQ=255)
                                                                                                                        aTAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACg                             >  4:240428/1‑149 (MQ=255)
                                                                                                                         tAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACgg                            <  3:553100/149‑1 (MQ=255)
                                                                                                                            ccATCGATTACCCTTTGCTGCAATTTGCTGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTAc                                                                          >  4:358699/1‑100 (MQ=255)
                                                                                                                            ccATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTAc                                                                          <  3:358699/100‑1 (MQ=255)
                                                                                                                             cATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGc                        >  2:539456/1‑149 (MQ=255)
                                                                                                                              aTCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCg                       <  2:496547/149‑1 (MQ=255)
                                                                                                                                 gATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTc                    <  4:224707/149‑1 (MQ=255)
                                                                                                                                  aTTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCt                   >  3:562308/1‑149 (MQ=255)
                                                                                                                                     accaattGCTGCAATTTGCAGCAACAACCACGACAGTGAAATTCTTGTGCCCTGGTAAACCAATTTCTGAATGCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGACATCTCATCCGATGCAACGCCACCACTGCGATCTgct                >  4:223112/6‑147 (MQ=255)
                                                                                                                                     aCCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGgt                <  4:127608/149‑1 (MQ=255)
                                                                                                                                      cccTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGAc                                                                                   >  1:196350/1‑81 (MQ=255)
                                                                                                                                      cccTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGAc                                                                                   <  2:196350/81‑1 (MQ=255)
                                                                                                                                      cccTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGgtt               <  4:443866/149‑1 (MQ=255)
                                                                                                                                        cTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGgttgt             >  3:230973/1‑149 (MQ=255)
                                                                                                                                        atttGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGgttgt             <  1:221073/148‑1 (MQ=255)
                                                                                                                                          ttGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGaa                                                              >  1:504329/1‑98 (MQ=255)
                                                                                                                                          ttGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGaa                                                              <  2:504329/98‑1 (MQ=255)
                                                                                                                                          ttGCTGCAAATTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACtt                                                           <  4:444451/101‑1 (MQ=255)
                                                                                                                                          ttGCTGCAAATTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTc           >  1:411652/1‑149 (MQ=255)
                                                                                                                                              tgcAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTCATcc       <  3:408952/149‑1 (MQ=255)
                                                                                                                                                 aaTTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTCATCCGGa    <  1:247843/149‑1 (MQ=255)
                                                                                                                                                 aaTTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTCATCCGGa    <  4:108813/149‑1 (MQ=255)
                                                                                                                                                   tttGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTCATCCGGATa  <  3:45126/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
AGAGAATGTTAAGCACATGCATAACGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTCATCCGGATA  >  NC_000913/4551275‑4551570

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: