Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	499,460	G→T	6.7%	L172I (CTC→ATC) ‡	aes ←	acetyl esterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	499,460	0	G	T	6.7%	207.7 / 5.2	60	L172I (CTC→ATC) ‡	aes	acetyl esterase
Reads supporting (aligned to +/- strand):  ref base G (25/31);  new base T (2/2);  total (27/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.03e-01

CTGTTGCGTTAAGCCATCCCAGACACCGCCCAACAGACGACGAGTCACGGAATCCCGTAATCCGTAAAGCCCATACCACAGCAAAACGCCCGCAACTTTACCGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTTCCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAA  >  NC_000913/499317‑499608                                                                                                                                                |                                                                                                                                                     cTGTTGCGTTAAGCCATCCCAGACACCGCCCAACAGACGACGAGTCACGGAATCCCGTAATCCGTAAAGCCCATACCACAGCAAAACGCCCGCAACTTTACCGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCa                                                                                                                                                 <  4:211431/149‑1 (MQ=255)  tGTTGCGTTAAGCCATCCCAGACACCGCCCAACAGACGACGAGTCACGGAATCCCGTAATCCGTAAAGCCCATACCACAGCAAAACGCCCGCAACTTTACCGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAg                                                                                                                                                >  4:578019/1‑149 (MQ=255)               cATCCCAGACACCGCCCAACAGACGACGAGTCACGGAATCCCGTAATCCGTAAAGCCCATACCACAGCAAAACGCCCGCAACTTTACCGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCg                                                                                                                                   >  4:481036/1‑149 (MQ=255)                aTCCCAGACACCGCCCAACAGACGACGAGTCACGGAATCCCGTAATCCGTAAAGCCCATACCACAGCAAAACGCCCGCAACTTTACCGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCgg                                                                                                                                  >  1:158780/1‑149 (MQ=255)                        cacCGCCCAACAGACGACGAGTCACGGAATCCCGTAATCCGTAAAGCCCATACCACAGCAAAACGCCCGCAACTTTACCGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCg                                                                                                                          <  4:72864/149‑1 (MQ=255)                               cAACAGACGACGAGTCACGGAATCCCGTAATCCGTATAGCCCATACCACAGCAAAACGCCCGCAACTTTACCGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGc                                                                                                                   <  3:390494/149‑1 (MQ=255)                               cAACAGACGACGAGTCACGGAATCCCGTAATCCGTAAAGCCCATACCACAGCAAAACGCCCGCAACTTTACCGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGc                                                                                                                   <  3:220494/149‑1 (MQ=255)                                aaCAGACGACGAGTCACGGAATCCCGTAATCCGTAAAGCCCATACCACAGCAAAACGCCCGCAACTTTACCGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAgc                                                                                                                                                     <  3:164498/114‑1 (MQ=255)                                aaCAGACGACGAGTCACGGAATCCCGTAATCCGTAAAGCCCATACCACAGCAAAACGCCCGCAACTTTACCGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAgc                                                                                                                                                     >  4:164498/1‑114 (MQ=255)                                aaCAGACGACGAGTCACGGAATCCCGTAATCCGTAAAGCCCATACCACAGCAAAACGCCCGCAACTTTACCGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGcc                                                                                                                  >  3:415485/1‑149 (MQ=255)                                   aGACGACGAGTCACGGAATCCCGTAATCCGTAAAGCCCATACCACAGCAAAACGCCCGCAACTTTACCGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAAt                                                                                                               >  4:287269/1‑149 (MQ=255)                                    gacgacGAGTCACGGAATCCCGTAATCCGTAAAGCCCATACCACAGCAAAACGCCCGCAACTTTACCGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATg                                                                                                              >  2:265504/1‑149 (MQ=255)                                       gacgaGTCACGGAATCCCGTAATCCGTAAAGCCCATACCACAGCAAAACGCCCGCAACTTTACCGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCgg                                                                                                           <  4:271304/149‑1 (MQ=255)                                             tCACGGAATCCCGTAATCCGTAAAGCCCATACCACAGCAAAACGCCCGCAACTTTACCGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACata                                                                                                     >  4:205462/1‑149 (MQ=255)                                               aCGGAATCCCGTAATCCGTAAAGCCCATACCACAGCAAAACGCCCGCAACTTTACCGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGCTCGCCAgt                                                                                                                                               >  1:28941/1‑104 (MQ=255)                                               aCGGAATCCCGTAATCCGTAAAGCCCATACCACAGCAAAACGCCCGCAACTTTACCGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGCTCGCCAgt                                                                                                                                               <  2:28941/105‑2 (MQ=255)                                               aCGGAATCCCGTAATCCGTAAAGCCCATACCACAGCAAAACGCCCGCAACTTTACCGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGcc                                                                                                                  <  2:451463/134‑1 (MQ=255)                                               aCGGAATCCCGTAATCCGTAAAGCCCATACCACAGCAAAACGCCCGCAACTTTACCGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGcc                                                                                                                  >  1:451463/1‑134 (MQ=255)                                               aCGGAATCCCGTAATCCGTAAAGCCCATACCACAGCAAAACGCCCGCAACTTTACCGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACat                                                                                                      >  2:144615/1‑146 (MQ=255)                                               aCGGAATCCCGTAATCCGTAAAGCCCATACCACAGCAAAACGCCCGCAACTTTACCGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACat                                                                                                      <  1:144615/146‑1 (MQ=255)                                                  gAATCCCGTAATCCGTAAAGCCCATACCACAGCAAAACGCCCGCAACTTTACCGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATAttgat                                                                                                <  3:481036/149‑1 (MQ=255)                                                  gAATCCCGTAATCCGTAAAGCCCATACCACAGCAAAACGCCCGCAACTTTACCGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATAttgat                                                                                                <  4:557036/149‑1 (MQ=255)                                                   aaTCCCGTAATCCGTAAAGCCCATACCACAGCAAAACGCCCGCAACTTTACCGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATAttgatt                                                                                               <  3:178771/149‑1 (MQ=255)                                                      cccGTAATCCGTAAAGCCCATACCACAGCAAAACGCCCGCAACTTTACCGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATAttgatttga                                                                                            <  1:13280/149‑1 (MQ=255)                                                       ccGTAATCCGTAAAGCCCATACCACAGCAAAACGCCCGCAACTTTACCGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATAttgatttgat                                                                                           >  4:175203/1‑149 (MQ=255)                                                           aaTCCGTAAAGCCCATACCACAGCAAAACGCCCGCAACTTTACCGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAAtc                                                                                       <  1:265504/149‑1 (MQ=255)                                                            aTCCGTAAAGCCCATACCACAGCAAAACGCCCGCAACTTTACCGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATAttgatttga                                                                                            <  4:544914/143‑1 (MQ=255)                                                            aTCCGTAAAGCCCATACCACAGCAAAACGCCCGCAACTTTACCGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATAttgatttga                                                                                            >  3:544914/1‑143 (MQ=255)                                                              ccGTAAAGCCCATACCACAGCAAAACGCCCGCAACTTTACCGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAAtcctc                                                                                    <  4:367525/149‑1 (MQ=255)                                                                gTAAAGCCCATACCACAGCAAAACGCCCGCAACTTTACCGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCaaa                                                                                                                     >  3:132138/1‑114 (MQ=255)                                                                gTAAAGCCCATACCACAGCAAAACGCCCGCAACTTTACCGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCaaa                                                                                                                     <  4:132138/114‑1 (MQ=255)                                                                gTAAAGCCCATACCACAGCAAAACGCCCGCAACTTTACCGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCg                                                                                  >  3:529183/1‑149 (MQ=255)                                                                   aaGCCCATACCACAGCAAAACGCCCGCAACTTTACCGCAATCGATCTGTTTATCTCGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCct                                                                               <  3:505724/149‑1 (MQ=255)                                                                      cccATACCACAGCAAAACGCCCGCAACTTTACCGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCctgct                                                                            >  4:131124/1‑149 (MQ=255)                                                                            ccACAGCAAAACGCCCGCAACTTTACCGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGa                                                                      >  1:355630/1‑149 (MQ=255)                                                                               caGCAAAACGCCCGCAACTTTACCGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAAt                                                                   <  1:270537/149‑1 (MQ=255)                                                                                aGCAAAACGCCCGCAACTTTACCGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATa                                                                  >  3:406806/1‑149 (MQ=255)                                                                                      aCGCCCGCAACTTTACCGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAacaaca                                                            <  1:365222/149‑1 (MQ=255)                                                                                        gCCCGCAACTTTACCGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAag                                                          >  4:489724/1‑149 (MQ=255)                                                                                         cccGCAACTTTACCGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAagc                                                         <  2:28533/149‑1 (MQ=255)                                                                                              aaCTTTACCGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGTAGCca                                                    >  4:377303/1‑149 (MQ=255)                                                                                                  ttACCGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAAt                                                <  4:415485/149‑1 (MQ=255)                                                                                                               tCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTTCCTCTATCGCt                                   >  1:243588/1‑149 (MQ=255)                                                                                                               tCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTTCCTCTATCGCt                                   <  3:284038/149‑1 (MQ=255)                                                                                                                 tGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTTCCTCTATCGCTTg                                 >  3:376432/1‑149 (MQ=255)                                                                                                                  gTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTTCCTCTATCGCTTGc                                <  3:157156/149‑1 (MQ=255)                                                                                                                  gTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTTCCTCTATCGCTTGc                                >  2:407291/1‑149 (MQ=255)                                                                                                                   tttATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTTCCTCTATCGCTTGCg                               >  2:570877/1‑149 (MQ=255)                                                                                                                      aTCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTTCCTCTATCGCTTGCGGaa                            >  3:186781/1‑149 (MQ=255)                                                                                                                              aCCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTTCCTCTATCGCTTGCGGAAAACGCGCt                    <  3:511756/149‑1 (MQ=255)                                                                                                                                    aCGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTTCCTCTATCGCTTGCGGAAAACGCGCTTCAGGt              <  2:158780/149‑1 (MQ=255)                                                                                                                                     catgcTGGCGCTCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTTcc                                            <  2:476474/114‑1 (MQ=255)                                                                                                                                     catgcTGGCGCTCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTTcc                                            >  1:476474/5‑118 (MQ=255)                                                                                                                                      gCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGaaa                                                                    >  3:164491/1‑93 (MQ=255)                                                                                                                                      gCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGaaa                                                                    <  4:164491/93‑1 (MQ=255)                                                                                                                                            gCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTTCCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGgt      <  1:564435/149‑1 (MQ=255)                                                                                                                                            gCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTTCCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGgt      <  1:396370/149‑1 (MQ=255)                                                                                                                                            gCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTTCCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGgt      <  3:205462/149‑1 (MQ=255)                                                                                                                                            gCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTTCCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGgt      <  3:287269/149‑1 (MQ=255)                                                                                                                                            gCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTTCCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGgt      <  3:3452/149‑1 (MQ=255)                                                                                                                                              gAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTTc                                             >  2:45283/1‑108 (MQ=255)                                                                                                                                              gAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTTc                                             <  1:45283/108‑1 (MQ=255)                                                                                                                                                gcgcCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTGCGCCTGCTGGTGGAAATAACAGCAAGCAGCCACAAGTTCCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTaa  <  1:566274/149‑1 (MQ=255)                                                                                                                                                gcgcCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGAATTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTTCCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTaa  <  3:377303/149‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                     CTGTTGCGTTAAGCCATCCCAGACACCGCCCAACAGACGACGAGTCACGGAATCCCGTAATCCGTAAAGCCCATACCACAGCAAAACGCCCGCAACTTTACCGCAATCGATCTGTTTATCACGCAACCACAACGCACTGGCGAGCGCCAGCATGGCACCTGCGGAATCACCGGCAAAGCCAATGCGGGACATATTGATTTGATAATCCTCCGCCTGCTGGTGGAAATAACAACAAGCAGCCACAATTTCCTCTATCGCTTGCGGAAAACGCGCTTCAGGTGAAAGGGTGTAA  >  NC_000913/499317‑499608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑