Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 615,275 T→A 5.4% G373G (GGT→GGA entF → enterobactin synthase multienzyme complex component, ATP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913615,2750TA 5.4% 251.6 / 3.4 74G373G (GGT→GGAentFenterobactin synthase multienzyme complex component, ATP‑dependent
Reads supporting (aligned to +/- strand):  ref base T (38/32);  new base A (2/2);  total (40/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01

AACGTTACGATGCCGAACAAATTGTCCGTGACAGCGGGCGAGCGGCAGGTGATGAACCGCTGTTTGGTCCGGTACTCAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTGAACGCCTGAAAATGCTGATTGCCCAGTTCGCCGCGG  >  NC_000913/615127‑615423
                                                                                                                                                    |                                                                                                                                                    
aaCGTTACGATGCCGAACAAATTGTCCGTGACAGCGGGCGAGCGGCAGGTGATGAACCGCTGTTTGGTCCGGTACTCAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAAccggt                                                                                                                                                      >  2:199298/1‑149 (MQ=255)
   gTTACGATGCCGAACAAATTGTCCGTGACAGCGGGCGAGCGGCAGGTGATGAACCGCTGTTTGGTCCGGTACTCAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAAccggtccg                                                                                                                                                   >  4:78578/1‑149 (MQ=255)
   gTTACGATGCCGAACAAATTGTCCGTGACAGCGGGCGAGCGGCAGGTGATGAACCGCTGTTTGGTCCGGTACTCAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAAccggtccg                                                                                                                                                   <  3:27555/149‑1 (MQ=255)
      aCGATGCCGAACAAATTGTCCGTGACAGCGGGCGAGCGGCAGGTGATGAACCGCTGTTTGGTCCGGTACTCAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGtt                                                                                                                                                <  1:146491/149‑1 (MQ=255)
        gATGCCGAACAAATTGTCCGTGACAGCGGGCGAGCGGCAGGTGATGAACCGCTGTTTGGTCCGGTACTCAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTaa                                                                                                                                              <  3:78578/149‑1 (MQ=255)
        gATGCCGAACAAATTGTCCGTGACAGCGGGCGAGCGGCAGGTGATGAACCGCTGTTTGGTCCGGTACTCAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTaa                                                                                                                                              >  2:143870/1‑149 (MQ=255)
        gATGCCGAACAAATTGTCCGTGACAGCGGGCGAGCGGCAGGTGATGAACCGCTGTTTGGTCCGGTACTCAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTaa                                                                                                                                              <  1:48821/149‑1 (MQ=255)
           gCCGAACAAATTGTCCGTGACAGCGGGCGAGCGGCAGGTGATGAACCGCTGTTTGGTCCGGTACTCAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGa                                                                                                                                           >  2:29074/1‑149 (MQ=255)
            ccGAACAAATTGTCCGTGACAGCGGGCGAGCGGCAGGTGATGAACCGCTGTTTGGTCCGGTACTCAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGAc                                                                                                                                          <  3:334211/149‑1 (MQ=255)
             cGAACAAATTGTCCGTGACAGCGGGCGAGCGGCAGGTGATGAACCGCTGTTTGGTCCGGTACTCAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGAcc                                                                                                                                         >  4:472090/1‑149 (MQ=255)
              gAACAAATTGTCCGTGACAGCGGGCGAGCGGCAGGTGATGAACCGCTGTTTGGTCCGGTACTCAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCt                                                                                                                                        >  1:116058/1‑149 (MQ=255)
               aaCAAATTGTCCGTGACAGCGGGCGAGCGGCAGGTGATGAACCGCTGTTTGGTCCGGTACTCAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCtt                                                                                                                                       >  2:130258/1‑149 (MQ=255)
                  aaaTTGTCCGTGACAGCGGGCGAGCGGCAGGTGATGAACCGCTGTTTGGTCCGGTACTCAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGaa                                                                                                                                    <  4:14414/149‑1 (MQ=255)
                  aaaTTGTCCGTGACAGCGGGCGAGCGGCAGGTGATGAACCGCTGTTTGGTCCGGTACTCAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGaa                                                                                                                                    <  4:226012/149‑1 (MQ=255)
                       gTCCGTGACAGCGGGCGAGCGGCAGGTGATGAACCGCTGTTTGGTCCGGTACTCAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGc                                                                                                                               <  3:185456/149‑1 (MQ=255)
                           gTGACAGCGGGCGAGCGGCAGGTGATGAACCGCTGTTTGGTCCGGTACTCAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCTg                                                                                                                           >  2:399086/1‑149 (MQ=255)
                           gTGACAGCGGGCGAGCGGCAGGTGATGAACCGCTGTTTGGTCCGGTACTCAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCATGAACTGGCCCTg                                                                                                                           >  4:370777/1‑149 (MQ=255)
                                aGCGGGCGAGCGGCAGGTGATGAACCGCTGTTTGGTCCGGTACTCAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCTGTTccc                                                                                                                      >  2:150953/1‑149 (MQ=255)
                                   gggCGAGCGGCAGGTGATGAACCGCTGTTTGGTCCGGTACTCAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCTGTTCCCGGa                                                                                                                   <  2:44235/149‑1 (MQ=255)
                                       gAGCGGCAGGTGATGAACCGCTGTTTGGGCCGGTACTCAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCTGTTCCGGGATGTa                                                                                                               >  3:503184/1‑149 (MQ=255)
                                           ggCAGGTGATGAACCGCTGTTTGGTCCGGTACTCAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCTGTTCCCGGATGTACACg                                                                                                           >  2:302843/1‑149 (MQ=255)
                                              aGGTGATGAACCGCTGTTTGGTCCGGTACTCAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCTGTTCCCGGATGTACACGGTg                                                                                                        <  3:138058/149‑1 (MQ=255)
                                                 tgatgaACCGCTGTTTGGTCCGGTACTCAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCTGTTCCCGGATGTACACGGTGAtt                                                                                                     <  1:253908/149‑1 (MQ=255)
                                                  gatgaACCGCTGTTTGGTCCGGTACTCAATATCACGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCTGTTCCCGGATGTACACGGTGAttt                                                                                                    <  1:488664/149‑1 (MQ=255)
                                                   atgaACCGCTGTTTGGTCCGGTACTCAATATCAAGGTATTTGATTACCAACTGGATATTCGTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCTGTTCCCGGATGTACACGGTGATTTg                                                                                                   >  3:339022/1‑149 (MQ=255)
                                                    tgaACCGCTGTTTGGTCCGGTACTCAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCTGTTCCCGGATGTACACGGTGATTTGa                                                                                                  <  3:427284/149‑1 (MQ=255)
                                                    tgaACCGCTGTTTGGTCCGGTACTCAAGATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAACCCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCTGTTCCCGGATGTACACGGTGATTTGa                                                                                                  <  1:143870/149‑1 (MQ=255)
                                                        ccGCTGTTTGGTCCGGTACTCAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCTGTTCCCGGATGTACACGGTGATTTGAGTAt                                                                                              >  1:586461/1‑149 (MQ=255)
                                                        ccGCTGTTTGGTCCGGTACTCAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCTGTTCCCGGATGTACACGGTGATTTGAGTAt                                                                                              >  3:393522/1‑149 (MQ=255)
                                                         cGCTGTTTGGTCCGGTACTCAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCTGTTCCCGGATGTACACGGTGATTTGAGTAtt                                                                                             >  3:106451/1‑149 (MQ=255)
                                                          gCTGTTTGGTCCGGTACTCAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTg                                                                                            >  4:133460/1‑149 (MQ=255)
                                                               ttGGTCCGGTACTCAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATc                                                                                       <  3:301958/149‑1 (MQ=255)
                                                                     cGGTACTCAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGcc                                                                                 >  2:582789/1‑149 (MQ=255)
                                                                       gTACTCAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCaa                                                                               >  2:536096/1‑149 (MQ=255)
                                                                           tCAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCTGTTCCCGGATGTACACGGTGATTTGAGTAtt                                                                                             <  1:297149/131‑1 (MQ=255)
                                                                           tCAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCTGTTCCCGGATGTACACGGTGATTTGAGTAtt                                                                                             >  2:297149/1‑131 (MQ=255)
                                                                           tCAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATaaa                                                                           <  4:549217/149‑1 (MQ=255)
                                                                           tCAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATaaa                                                                           >  3:563253/1‑149 (MQ=255)
                                                                             aaTATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACa                                                                         >  2:560446/1‑149 (MQ=255)
                                                                              atatCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAg                                                                        <  2:495217/149‑1 (MQ=255)
                                                                              atatCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAg                                                                        <  1:536096/149‑1 (MQ=255)
                                                                                   aaGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTa                                                                   >  1:519660/1‑149 (MQ=255)
                                                                                   aaGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTa                                                                   <  3:63780/149‑1 (MQ=255)
                                                                                    aGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCTGTTCCCGGATGTaca                                                                                                             >  3:158894/1‑106 (MQ=255)
                                                                                    aGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCTGTTCCCGGATGTaca                                                                                                             <  4:158894/106‑1 (MQ=255)
                                                                                      gTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGa                                                                >  4:5393/1‑149 (MQ=255)
                                                                                       tATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGAt                                                               <  2:427234/149‑1 (MQ=255)
                                                                                             aTTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTaa                                                                                                                                              >  2:435742/1‑64 (MQ=255)
                                                                                             aTTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTaa                                                                                                                                              <  1:435742/64‑1 (MQ=255)
                                                                                                aCCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACTCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCACTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACg                                                      >  4:356796/1‑149 (MQ=255)
                                                                                                     ccggATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAAt                                                 <  2:172598/147‑1 (MQ=255)
                                                                                                      tGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATc                                                <  2:418706/149‑1 (MQ=255)
                                                                                                              ccTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGc                                        <  2:519660/149‑1 (MQ=255)
                                                                                                               cTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCt                                       >  2:303773/1‑149 (MQ=255)
                                                                                                               cTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCt                                       >  1:281241/1‑149 (MQ=255)
                                                                                                               cTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCt                                       >  1:223910/1‑149 (MQ=255)
                                                                                                                tGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAAt                                                 >  4:482099/1‑138 (MQ=255)
                                                                                                                tGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAAt                                                 <  3:482099/138‑1 (MQ=255)
                                                                                                                tGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTg                                      >  3:127187/1‑149 (MQ=255)
                                                                                                                tGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTg                                      >  1:455234/1‑149 (MQ=255)
                                                                                                                tGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCTGTTCCCGGATGTACACGGTGATTTGAGGATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTg                                      >  2:97487/1‑149 (MQ=255)
                                                                                                                     ttCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTGAACGc                                 >  1:576919/1‑149 (MQ=255)
                                                                                                                     ttCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTGAACGc                                 >  1:410410/1‑149 (MQ=255)
                                                                                                                      tCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTGAACGcc                                <  3:70162/149‑1 (MQ=255)
                                                                                                                            gcAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTGAACGCCTGaaaa                          >  2:344367/1‑149 (MQ=255)
                                                                                                                              aaaCCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCGATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTGAACGCCTGAAAATg                        <  4:106451/149‑1 (MQ=255)
                                                                                                                               aaCCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTGAACGCCTGAAAATGc                       <  1:477567/149‑1 (MQ=255)
                                                                                                                                  ccATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTGAACGCCTGAAAATGCTGa                    >  4:22892/1‑149 (MQ=255)
                                                                                                                                      aCCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTGAACGCCTGAAAATGCTGATTGc                >  4:331018/1‑149 (MQ=255)
                                                                                                                                      aCCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAAGCCAGCATGCTGAACGCCTGAAAATGCTGATTGc                <  2:576919/149‑1 (MQ=255)
                                                                                                                                      aCCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCGCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTGAACGCCTGAAAATGCTGATTGc                <  2:410410/149‑1 (MQ=255)
                                                                                                                                         tcattaaCCGGACCGGTTAATGATCTTGAACTGGCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCt                                       <  3:237230/118‑1 (MQ=255)
                                                                                                                                         tcattaaCCGGACCGGTTAATGACCTTGAACTGGCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCt                                       >  4:237230/6‑123 (MQ=255)
                                                                                                                                         cTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTGAACGCCTGAAAATGCTGATTGCCCa             >  3:494910/1‑149 (MQ=255)
                                                                                                                                          cattaaCCGGACCGGTTAATGACCTTGAACTGGCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCt                                       <  1:236137/118‑1 (MQ=255)
                                                                                                                                          cattaaCCGGACCGGTTAATGACCTTGAACTGGCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCt                                       >  2:236137/5‑122 (MQ=255)
                                                                                                                                             cAACCGGTCCGGTTAATGACCTTGAACTGGCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTGAACGCCTGAAAATGCTGATTGCCCAGTTc         <  1:114853/149‑1 (MQ=255)
                                                                                                                                                 cggtccggtTAATGACCTTGAACTGGCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTGAACGCCTGAAAATGCTGATTGCCCAGTTcgccg     <  3:363475/149‑1 (MQ=255)
                                                                                                                                                    tccggtTAATGACCTTGAACTGGCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTGAACGCCTGAAAATGCTGATTGCCCAGTTCGCCGCgg  <  4:436254/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AACGTTACGATGCCGAACAAATTGTCCGTGACAGCGGGCGAGCGGCAGGTGATGAACCGCTGTTTGGTCCGGTACTCAATATCAAGGTATTTGATTACCAACTGGATATTCCTGATGTTCAGGCGCAAACCCATACCCTGGCAACCGGTCCGGTTAATGACCTTGAACTGGCCCTGTTCCCGGATGTACACGGTGATTTGAGTATTGAGATCCTCGCCAATAAACAGCGTTACGATGAGCCAACGTTAATCCAGCATGCTGAACGCCTGAAAATGCTGATTGCCCAGTTCGCCGCGG  >  NC_000913/615127‑615423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: