Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 691,862 A→T 6.9% intergenic (‑78/+12) ybeX ← / ← ybeY putative ion transport/ssRNA‑specific endoribonuclease; 16S rRNA 3' end maturation and quality control co‑endoribonuclease working with RNase R; rRNA transcription antitermination factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913691,8620AT 6.9% 245.3 / 8.1 72intergenic (‑78/+12)ybeX/ybeYputative ion transport/ssRNA‑specific endoribonuclease; 16S rRNA 3' end maturation and quality control co‑endoribonuclease working with RNase R; rRNA transcription antitermination factor
Reads supporting (aligned to +/- strand):  ref base A (30/37);  new base T (2/3);  total (32/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.13e-01

TGAGTAACAGGGAGAAAAATCCCTTCTTGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACAAATGCAGACTGCCGTGCACCAC  >  NC_000913/691715‑692008
                                                                                                                                                   |                                                                                                                                                  
tGAGTAACAGGGAGAAAAATCCCTTCTTGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAAt                                                                                                                                                   >  1:494812/1‑149 (MQ=255)
   gTAACAGGGAGAAAAATCCCTTCTTGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGGCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGcc                                                                                                                                                <  3:231343/149‑1 (MQ=255)
    tAACAGGGAGAAAAATCCCTTCTTGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCt                                                                                                                                               <  4:577479/149‑1 (MQ=255)
    tAACAGGGAGAAAAATCCCTTCTTGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCt                                                                                                                                               >  1:199964/1‑149 (MQ=255)
    tAACAGGGAGAAAAATCCCTTCTTGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCt                                                                                                                                               <  3:528925/149‑1 (MQ=255)
           gagaAAAATCCCTTCTTGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAat                                                                                                                                        >  3:34453/1‑149 (MQ=255)
           gagaAAAATCCCTTCTTGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGGCATTTGACTGGCagac                                                                                                                                        <  2:478884/149‑4 (MQ=255)
            agaAAAATCCCTTCTTGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAatt                                                                                                                                       >  2:141665/1‑149 (MQ=255)
               aaaaTCCCTTCTTGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAattatt                                                                                                                                    <  3:297829/149‑1 (MQ=255)
               aaaaTCCCTTCTTGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAattatt                                                                                                                                    <  3:20523/149‑1 (MQ=255)
                  aTCCCTTCTTGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTAttctt                                                                                                                                 >  2:3907/1‑149 (MQ=255)
                    cccTTCTTGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTAttctttc                                                                                                                               <  4:232314/149‑1 (MQ=255)
                      cttcttGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTtctc                                                                                                                             >  2:216006/1‑149 (MQ=255)
                      cttcttGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTtctc                                                                                                                             <  2:316706/149‑1 (MQ=255)
                      cttcttGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTtctc                                                                                                                             >  3:175221/1‑149 (MQ=255)
                        tcttGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCgg                                                                                                                           <  2:199964/149‑1 (MQ=255)
                            gttgCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAAt                                                                                                                       <  4:219416/149‑1 (MQ=255)
                                    tCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGAt                                                                                                               <  4:257002/149‑1 (MQ=255)
                                      gtgtCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATcc                                                                                                             <  4:34453/149‑1 (MQ=255)
                                      gtgtCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATcc                                                                                                             <  4:245458/149‑1 (MQ=255)
                                          cACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCAt                                                                                                         <  1:186440/149‑1 (MQ=255)
                                           actactGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATa                                                                                                        >  2:371109/1‑149 (MQ=255)
                                           actactGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATa                                                                                                        >  1:242442/1‑149 (MQ=255)
                                           actactGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATa                                                                                                        >  1:237922/1‑149 (MQ=255)
                                             tactGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGc                                                                                                      >  4:62334/1‑149 (MQ=255)
                                                  tgtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCaga                                                                                                 <  1:371109/149‑1 (MQ=255)
                                                  tgtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCaga                                                                                                 >  1:484557/1‑149 (MQ=255)
                                                       aTTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGagcaag                                                                                            <  2:237922/149‑1 (MQ=255)
                                                              tcgCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAAtc                                                                                     >  4:325976/1‑149 (MQ=255)
                                                                gCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTc                                                                                                           >  2:346929/1‑125 (MQ=255)
                                                                gCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTc                                                                                                           <  1:346929/125‑1 (MQ=255)
                                                                 cTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATctct                                                                                  <  1:218897/149‑1 (MQ=255)
                                                                  tCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTg                                                                                 >  2:126780/1‑149 (MQ=255)
                                                                      ggCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTc                                                                             >  2:321339/1‑149 (MQ=255)
                                                                             tATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCt                                                                      >  4:48890/1‑149 (MQ=255)
                                                                              aTGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCtt                                                                     <  3:48890/149‑1 (MQ=255)
                                                                                        aTGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATttcttc                                                           <  3:62334/149‑1 (MQ=255)
                                                                                        aTGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATttcttc                                                           <  4:374777/149‑1 (MQ=255)
                                                                                             agttaATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCtt                                                      >  4:224989/1‑149 (MQ=255)
                                                                                                  aTCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGATGGCTTCCATTTCTTCTGCTTCGTCa                                                 >  4:526020/1‑149 (MQ=255)
                                                                                                   tCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCAt                                                >  1:511015/1‑149 (MQ=255)
                                                                                                    cAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATc                                               <  2:52954/149‑1 (MQ=255)
                                                                                                     aaaaCGCCGTCGTTAATCACCAACGGCGTGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCt                                              >  3:545028/1‑149 (MQ=255)
                                                                                                     aaaaCGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCt                                              >  3:396340/1‑149 (MQ=255)
                                                                                                         cGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGa                                          <  2:4189/149‑1 (MQ=255)
                                                                                                          gCCGTCTTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGAt                                         <  3:282742/149‑1 (MQ=255)
                                                                                                            cgtcgtTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGAtgt                                       >  3:96140/1‑149 (MQ=255)
                                                                                                             gtcgtTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCt                                              <  1:312460/141‑1 (MQ=255)
                                                                                                             gtcgtTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCt                                              >  2:312460/1‑141 (MQ=255)
                                                                                                              tcgtTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGc                                                        >  1:338323/1‑130 (MQ=255)
                                                                                                              tcgtTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGc                                                        <  2:338323/130‑1 (MQ=255)
                                                                                                              tcgtTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGa                                     <  1:3907/149‑1 (MQ=255)
                                                                                                                      cACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAAcc                             <  1:126780/149‑1 (MQ=255)
                                                                                                                         ccgagaaagaataattTGCCAGGCATTTGACTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTaa                          <  4:287387‑M1/133‑1 (MQ=255)
                                                                                                                         cAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAATCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCTTCATCTTCGATGTGATCGTCACCTaa                          >  3:396787/1‑149 (MQ=255)
                                                                                                                         cAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTaa                          >  3:490017/1‑149 (MQ=255)
                                                                                                                          aaCGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAAc                         >  2:53522/1‑149 (MQ=255)
                                                                                                                              gcggGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACAAAt                     <  2:484557/149‑1 (MQ=255)
                                                                                                                              gcggGGACGTCTGCCAGTCAAATGCCTGGCAAATTACTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACAAAt                     <  4:396340/149‑1 (MQ=255)
                                                                                                                              aaagaataattTGCCAGGCATTTGACTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACAAAt                     >  3:287387‑M1/12‑149 (MQ=255)
                                                                                                                                ggggACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACAAATGc                   <  1:216006/149‑1 (MQ=255)
                                                                                                                                 gggACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACAAATGCa                  <  4:490017/149‑1 (MQ=255)
                                                                                                                                 gggACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACAAATGCa                  <  1:539387/149‑1 (MQ=255)
                                                                                                                                       ttTGCCAGGCATTTGACTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGagcaagc                                                                                           <  1:519695‑M1/68‑1 (MQ=255)
                                                                                                                                       ttTGCCAGGCATTTGACTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGagcaagc                                                                                           >  2:519695‑M1/3‑70 (MQ=255)
                                                                                                                                        cTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACAAATGCAGACTGcc           <  4:175221/149‑1 (MQ=255)
                                                                                                                                         TGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACAAATGCAGACTGCCg          <  1:13595‑M1/149‑1 (MQ=255)
                                                                                                                                           CCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATc                                   <  1:44385‑M1/122‑1 (MQ=255)
                                                                                                                                           CCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATc                                   >  2:44385‑M1/1‑122 (MQ=255)
                                                                                                                                             AGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACAAATGCAGACTGCCGTGCa      >  2:28234‑M1/1‑149 (MQ=255)
                                                                                                                                              GTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACAAATGCAGACTGCCGTGCAc     <  2:508152‑M1/149‑1 (MQ=255)
                                                                                                                                              GTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACAAATGCAGACTGCCGTGCAc     <  1:492616‑M1/149‑1 (MQ=255)
                                                                                                                                                 aaaTGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACAAATGCAGACTGCCGTGcaccac  >  2:203829/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TGAGTAACAGGGAGAAAAATCCCTTCTTGTTGCTTATCGTGTCACTACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGTTTCGAGGGCTTCCATTTCTTCTGCTTCGTCATCTTCGATGTGATCGTAACCTAACAAATGCAGACTGCCGTGCACCAC  >  NC_000913/691715‑692008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: