Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,117,027 C→A A66S (GCG→TCG)  glpK ← glycerol kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,117,0270CA98.1% 147.5 / NA 52A66S (GCG→TCG) glpKglycerol kinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (25/26);  minor base G (0/1);  total (25/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCAC  >  NC_000913/4116755‑4117295
                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                             
tGCACTCTCGCGAGGGCCTTCCACATGTTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATGTCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACa                                                                                                                                                                                                                                                                             <  4:159429/275‑1 (MQ=255)
tGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGTCTTGCCTGTTTCTTTTTCCCAGATAATAGGTGTTTCACGCTGGTTCGTAATACCGTTAGCTTCATTATGCTCGTAACTGAGATCGGTTTTCGACa                                                                                                                                                                                                                                                                             >  2:158717/1‑275 (MQ=255)
            agagCCTTCCACATGGTCGCGGATCCACTTTTCTTTGGTGCCAGAAAAGTATGGGTCAATCACCCGACCGGTATTGCCGCGGATCTAATCTTCTAAACCGTCACGTTATAAATGCCCGCAGCTTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGTTAGCTGCAATTTGATTGGAACTGATATCGGATTTCGACAGCACTTTTAcac                                                                                                                                                                                                                                                                 >  2:16878/1‑273 (MQ=255)
                 cTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGt                                                                                                                                                                                                                                                             <  2:134697/274‑1 (MQ=255)
                                    ccACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACa                                                                                                                                                                                                                                                                             >  2:140658/1‑239 (MQ=255)
                                        ttcacttTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCAc                                                                                                                                                                                                                                                                         <  4:140658/239‑1 (MQ=255)
                                          cgcttTGGTGCCAGAAAAGTACGGGTCAATCCCCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCa                                                                                                                                                                                                                                   <  3:77471/273‑1 (MQ=255)
                                          cacttTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCa                                                                                                                                                                                                                                   >  1:154679/1‑275 (MQ=255)
                                                 gggcaaGAAAAGTACGGGTCAATCACCAGAACGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTc                                                                                                                                                                                                                            <  1:6173/270‑1 (MQ=255)
                                                 gTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGAGGATATAATCTGTTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTc                                                                                                                                                                                                                            <  2:97675/275‑1 (MQ=255)
                                                                  gTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGGTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATTCCGCTAGCTGCAATTTGATCGGAACTGATATCGGCCTTCTAGCGCACTTCTACCAGCTTTGCGCTTTTGGTGGCCCCGATTTCCCTTGGGTCGATGTCTACCCCActtt                                                                                                                                                                                                           >  2:206948/1‑272 (MQ=255)
                                                                                   tATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCTTGTTCTACCCAACCTTGTTTTGGTGAGAt                                                                                                                                                                                              >  4:169905/1‑271 (MQ=255)
                                                                                          gcGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTGTGGGTGGCCCAGATTTCCATTGGGACGTGTTCTACCCAACCTGTTTTTGGGTAGATTTGCTCatat                                                                                                                                                                                    >  2:209846/1‑274 (MQ=255)
                                                                                             gATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATGGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCCTAAATTcgc                                                                                                                                                                                >  4:102747/1‑275 (MQ=255)
                                                                                                   aTCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCCCTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTTGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTccgcgtgcg                                                                                                                                                                          >  3:124730/1‑267 (MQ=255)
                                                                                                       tctAAACCGTCATGTTTTAAAATCTCGCAGCTTGCTGCGGTACGACGGCACTGCCAGAAAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGaca                                                                                                                                                                       <  4:158717/274‑1 (MQ=255)
                                                                                                       tctAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCTCAGATTTCCATTGGGTTGTGTTCTACCCAACCTGGTTTTGGGTAGCTTTGCTCAAATTCGCGCTTCGaca                                                                                                                                                                       >  2:201914/1‑274 (MQ=255)
                                                                                                        ctAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGTGTAGATTTGCTCAAATTCGCGCTGCGACACg                                                                                                                                                                     >  1:265649/1‑275 (MQ=255)
                                                                                                                tCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGTCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGt                                                                                                                                                                                                                                                 >  1:229530/1‑191 (MQ=255)
                                                                                                                tCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAg                                                                                                                                                                                                                                                                >  2:126909/1‑176 (MQ=255)
                                                                                                                    gTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTg                                                                                                                                                                                                                                                            <  4:126909/176‑1 (MQ=255)
                                                                                                                    gTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGcc                                                                                                                                                                                                                                             <  3:229529/191‑1 (MQ=255)
                                                                                                                             ggtCGCAGATTGCTGCGGTACGACGGCACTGCCAGTCAATGGCGTTATAGTTAGGCTTGCCGGTTTCTTTTTCCCAGTCAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCGAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa                                                                                                                                                <  2:78707/273‑1 (MQ=255)
                                                                                                                                cGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGt                                                                                                                                                                                                                                                 >  4:56796/1‑175 (MQ=255)
                                                                                                                                cGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTTTACCCAACCTGGTTTTGGGTAGATTTGCTCCAATTCGCCCTGCGACACGCTATTGATATTGGCATCTTGCTcc                                                                                                                                             >  4:90013/1‑275 (MQ=255)
                                                                                                                                    gATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGcc                                                                                                                                                                                                                                             <  2:56796/175‑1 (MQ=255)
                                                                                                                                                 cgacgGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGGTTTGCTCAAATTGGCGCTGCGACACGCTAATGATATTGGCATGGTGATCCATTACGTCcgcgca                                                                                                                              >  1:110061/1‑271 (MQ=255)
                                                                                                                                                    cgGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGc                                                                                                                         <  3:154678/274‑1 (MQ=255)
                                                                                                                                                         cTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGGTTTGCTCAAATTCGCGCTGCGACACGCTAAAGATATTGGCATCGGGATCCATTACGACCGTGCGGTGGCtggggg                                                                                                                   >  4:176556/1‑275 (MQ=255)
                                                                                                                                                            ccAGGCCATGGCGTTATTGGTAGGCTTTCCGGTTTCTTTTTCCCAGAAATTAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGgtggtg                                                                                                                 <  4:16878/274‑1 (MQ=255)
                                                                                                                                                                        attcttgatcgGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCCCGCTGGTTCGTAATCCCGATAGCTGCAATTTGAGTGGAACTGAGATAGGATTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAgcg                                                                                                    <  2:157592/269‑1 (MQ=255)
                                                                                                                                                                           atagatagGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGTGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCaa                                                                                                 <  2:169905/275‑1 (MQ=255)
                                                                                                                                                                                 agGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGCTTTGCTCAAATTCGCGCTGCGACACGCCAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGatat                                                                                           >  1:236799/1‑275 (MQ=255)
                                                                                                                                                                                   gCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACAGGCTAATGATATTGGAATTGTGATCCTTTACGACCGCGCGGGAGCTGGCGGTGCCCTGGTCGAGCGCAACGatatat                                                                                         >  1:76615/1‑275 (MQ=255)
                                                                                                                                                                                       gCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCCTTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTCGATTTGCTCAAATTCGCGCTGCTACACGCTAATGATATTGGCTTCGTGATCCATTACGTACTGCCGGGATCTGGTGGTTCACTGGTCGAGGGACACGATGTCttttt                                                                                     >  2:181505/1‑275 (MQ=255)
                                                                                                                                                                                        ccGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGGGCGCAACGATATAtttttt                                                                                    >  2:51823/1‑275 (MQ=255)
                                                                                                                                                                                          tgTTTCTTTTTCCCAGACAATAGTGGTTTCACTCTGGTTAGTGATACCTATTGCTGCAATTTGATCGGAACTGGTATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAGATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATAtttttttt                                                                                  <  2:102747/274‑1 (MQ=255)
                                                                                                                                                                                          ggTTTTTTTTTCCCAGAAAATAGTGGTTTCACGCTGGTTCGTACTACCTGTAGCTGCAATTTGGTCGGAACTGATATCGGCTTTCGACAGCCCTTCTACCAGCGTGGAGCTTTGGTTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATAtttttttt                                                                                  <  4:257141/275‑1 (MQ=255)
                                                                                                                                                                                                   ttCACAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGAGGAACTGATTACGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGTTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATg                                                                          <  1:41346/274‑1 (MQ=255)
                                                                                                                                                                                                       aagcaactgtgggTTTGAGGCTGGTTGCGAAAACGGATGCCTGGAATTTGATCGGAACCGATATCGGCTTTCGACAGTACTTCGTCCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGAGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTaa                                                                     <  4:193478/264‑1 (MQ=255)
                                                                                                                                                                                                                gttgTTTCCCGCTGGTTCGTAATACCGATAGTTGCAATTTGGTCGGAACTTATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGt                                                            <  4:35103/272‑1 (MQ=255)
                                                                                                                                                                                                                gTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCTGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGGGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGt                                                            <  2:90013/275‑1 (MQ=255)
                                                                                                                                                                                                                  ggTTTCACGCTGGTTCGTAATACCGATTGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGCCATGTTTAATTGTCCCGTAg                                                          <  3:265648/275‑1 (MQ=255)
                                                                                                                                                                                                                   gTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATc                                                                                                                                                    >  3:240284/1‑185 (MQ=255)
                                                                                                                                                                                                                        aCGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGTGTGGCCCAGATTTCCATTGGTTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGTATCGTTATCCATTACGACCTCGTGGGCACTGGTGTTTCCCTGGTCGAGCGTAACGATATATTTGTTTTCAGTCATGTTTGATTTTCCCGTTGTCatat                                                    >  2:263723/1‑275 (MQ=255)
                                                                                                                                                                                                                                      taactgtAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGGAAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCCTTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCttt                                      <  2:141803/269‑1 (MQ=255)
                                                                                                                                                                                                                                               ctccAATGTGATCGGAACTGTTTTTGGCTTTCGACAGCACTTCTACCCGCGTGGAGCTTTGGGTTGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGa                             <  4:51823/272‑1 (MQ=255)
                                                                                                                                                                                                                                                  cAATTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAgg                          >  3:167001/1‑275 (MQ=255)
                                                                                                                                                                                                                                                    aTTTGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGacac                                                                                                                                                                      >  2:148215/1‑134 (MQ=255)
                                                                                                                                                                                                                                                       tGATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTGTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTtg                     <  1:214707/275‑1 (MQ=255)
                                                                                                                                                                                                                                                        gATCGGAACTGATATCGGCTTTCGACAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTa                                                                                                                                                                  <  4:148215/134‑1 (MQ=255)
                                                                                                                                                                                                                                                                          cTTTCGACAGAACTTCACACAGCGTGGGTGTTTGGGTAGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCGGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGTTATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCcac  <  1:124731/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                             
TGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCAC  >  NC_000913/4116755‑4117295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: