Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,318,095 C→G L594F (TTG→TTC rcsC ← hybrid sensory kinase in two‑component regulatory system with RcsB and YojN

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,318,0950CG96.2% 171.7 / NA 54L594F (TTG→TTCrcsChybrid sensory kinase in two‑component regulatory system with RcsB and YojN
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base G (27/24);  minor base T (1/1);  total (29/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCACCAGCCGATC  >  NC_000913/2317823‑2318364
                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                             
gtcaaCTGGCTGCCCATTCCCTGTTCTGACTCTACCGAGATCACGCCGTCCATCATGCTGAGCAGTTTTTCACACATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGaa                                                                                                                                                                                                                                                                             <  2:254912/272‑1 (MQ=255)
gTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCCCGAACATGCAAAACTATACAGCCGGTATAGGGGAATTTTATGGCGTTACGCaa                                                                                                                                                                                                                                                                             >  3:93629/1‑275 (MQ=255)
 taagctgtcttaccATTCCCGGTACTGAATCTACCGAGATATCGCCGACCATCATGATGATCAGGTTTTCACAAATCGCCAGACCCAGACCGCTCCACTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTACCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAAc                                                                                                                                                                                                                                                                            <  2:241335/263‑1 (MQ=255)
        gCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACCCGGATAGAGAGATAATCGCCATCCGCGCGAACATGCCAAACTATACAGCCGGTAACGGGGAATTTTATGGCGGTACTGAACAGGTTgg                                                                                                                                                                                                                                                                     >  4:254912/1‑275 (MQ=255)
         cTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGTCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCNGGAAATTACGCTGTACGCCCGTTCCGACCTGGACGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGAATCGCGGACACGGATAAAGAGACAATCGCCACCCGCGCGAACATGCAAACATAAAAAGCCGGTATCGGTGAATTTTATGGCGTGACTGCACAGGTTGga                                                                                                                                                                                                                                                                    >  4:150237/1‑275 (MQ=255)
           gCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGGCATCAGCGCGAACCAGCAAAAAGATACAGCCGGGATCGGTGAATATTATCGCGTTACAGAACAGGTTGgaga                                                                                                                                                                                                                                                                  >  2:74728/1‑275 (MQ=255)
                ttCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTGTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGAcc                                                                                                                                                                                                                                                             <  2:13594/275‑1 (MQ=255)
                               ctcccGAGAACTCGCCGTCAATCATGCTGATAAGTTTTCACCAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATAAAACAAGCGCACCACTTCTTTCGCCTGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATc                                                                                                                                                                                                                                              <  2:100996/272‑1 (MQ=255)
                                   caggaTATCGCCGTCAATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCACCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACTCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGt                                                                                                                                                                                                                                          <  2:241593/272‑1 (MQ=255)
                                      gaTATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCCCCATCCGCGCGAACCTGCAAAACTATACAGCCGGGATCGGGGAAGTTTATGGGCTTTCTGAACAGGTTGGTGATGACCTGGTGTAAACGGAACGGGTAGc                                                                                                                                                                                                                                       >  3:63646/1‑275 (MQ=255)
                                      gaTATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGAAAAAGAGCTAATCCCCACCAGCCCCAACATGCAAACCTATACAGCCGGTAACGGTGAATTTTGTGGCGTTACCGAACAGGGTGGAGATGACCTGCTGaaaaacaatgagggcgc                                                                                                                                                                                                                                       >  4:119758/1‑262 (MQ=255)
                                      gaTATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACAAGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTTAATTTTATGGCGTTAACTAACAGGTTGGAGATTACCTGCTGTAAACCGATCGGGTCga                                                                                                                                                                                                                                       >  3:236090/1‑274 (MQ=255)
                                        tatCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCa                                                                                                                                                                                                                                     >  3:36811/1‑275 (MQ=255)
                                            gCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGTTCCCCTGGACATTACGCTTTACGCCCGTCCCGCCCTGGAAGAAGGGATCAAACAAGCGCACCACTTCATTCGCCGGTATCCCCACTCCGGTATCGCGAACACGGATCGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAAAAGGTTGGAGAGTACATACTGTACATGCATCGGGTAGCCATTTa                                                                                                                                                                                                                                 >  4:66373/1‑275 (MQ=255)
                                            cccttCCATCATGCTGATCAGTTTTTCACAAATCGCCCGACCCCGACCGGTCCCCTGGAAATTACGCTGTCCGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGGGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTa                                                                                                                                                                                                                                 <  4:74728/271‑1 (MQ=255)
                                             ccGTCCATCATGCTGATCAGTTTTGCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTaa                                                                                                                                                                                                                                <  1:93629/275‑1 (MQ=255)
                                                  catcatGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCa                                                                                                                                                                                                                           <  2:48203/275‑1 (MQ=255)
                                                            aTCAGGTTTTCACAGACCGCCAGACCCACACCCGCCCCCTGGAAACTACGCTGTACGCCCGTTCCGAACTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAACCTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATc                                                                                                                                                                                                                 <  2:37687/275‑1 (MQ=255)
                                                                         acaTCGCCCGACCCGGACCGGTCCCCTGTACATTACGCTGTACGCCCATTCCGACCTGGAAGAAGGGATCAAACAAGCGAACCACTTCTTTCGCCGGTACCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACGATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAg                                                                                                                                                                                                    <  2:124367/273‑1 (MQ=255)
                                                                         aaaTCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAg                                                                                                                                                                                                    <  1:36811/275‑1 (MQ=255)
                                                                                 gggcccagaccGGTCCCCTGGAAATTACGCGGTAAGACCGTCCCACCCTGTAAGAAGGGATCAAACAAGCGCAACACTTCTTTCGCCGGTAGCCCCACGACGGTATCGCGAACACGGATAGAGAGATCATGGCCATCCGCGCGAACATGCAAACCTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACAGCCGGTTCAATAAAGCAGTACaa                                                                                                                                                                                            <  1:214972/272‑1 (MQ=255)
                                                                                             gTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCATACAAGCGCACCACTTCCTTCGCCGGTATCCCCACGCAGGTATCGCGAACACGGATAGCGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTTGTGAAAAGGTTGGAAATGACCTGCTTTAAACGCATCGGGTCGCCATTTAACGCCACTGGCACATCCGGTTCAATAAAGCAGTACCAGCCGAACTGCtt                                                                                                                                                                                >  1:266392/1‑275 (MQ=255)
                                                                                             gTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGCTAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGCATTTTATGGCGTTACTGAATAGGTTGGAGATGACCTGCTGTAAACGCATAGGGTCGCCATTTAAGGCCACTGGCACATCCGGCTCAAGAAAGCAGTACAAGCCTaaattctg                                                                                                                                                                                >  2:102392/1‑269 (MQ=255)
                                                                                              tCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATACTCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGGGAATTTTATGGCGGTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGTCCACTGGCACATCCGGTTCAATAAAGTATTACAAGCCTAACTGCTTg                                                                                                                                                                               >  3:212143/1‑275 (MQ=255)
                                                                                              tCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGGTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCAAATCAGGTTCAATAAAGCAGTACAAGCCTAACTACTTg                                                                                                                                                                               >  3:212602/1‑275 (MQ=255)
                                                                                                       aaTTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTaccacc                                                                                                                                                                      >  3:176901/1‑275 (MQ=255)
                                                                                                        aatACGCGGTACGCCCGTTCCGACCTGGACGAAGGGAGCAAACAAGCGCACCACTTCATTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTaccac                                                                                                                                                                       <  2:66373/271‑1 (MQ=255)
                                                                                                          tACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTCTGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCCTCGGGTCGCCATTCAAGGCCACTGGCACATCCGGTTCAATAAATCAGTACAAGCCTACCTGCTTCAGTACCCACAGc                                                                                                                                                                   >  4:235631/1‑275 (MQ=255)
                                                                                                                              aCCTTGAAGAAGGGAGCAAACAAGCGCACCAAGTCTTTCGCCGGTACCCCCACGCCGGTATGGCGAACACGGAAAGAGAGATGATCGCCATCCGGGCGAACATGCAAAACTATACGGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGGCGGCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGAt                                                                                                                                               <  3:266391/275‑1 (MQ=255)
                                                                                                                                        agGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCACCGGTACATAGTTTGCGGTGATGTGGTtcatc                                                                                                                                     >  2:232132/1‑275 (MQ=255)
                                                                                                                                         gggATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTacc                                                                                                                                                                         >  3:5384/1‑238 (MQ=255)
                                                                                                                                             tCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTAccacca                                                                                                                                                                     <  1:5384/238‑1 (MQ=255)
                                                                                                                                             tCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGATTGCGTACCACCCGCGGTAAATAGTTGGCGGAGATGGGGTTCATCAATTc                                                                                                                                >  4:86506/1‑275 (MQ=255)
                                                                                                                                             tCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTCATTGCGGACCACCAGCGGTAAATATTTGACGGTGATGTGGttgttccctt                                                                                                                                 >  4:266685/1‑266 (MQ=255)
                                                                                                                                                                     gacGGTATACCCACGCCGGTATCGCGAACAAGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGtt                                                                                                         <  2:36442/272‑1 (MQ=255)
                                                                                                                                                                          tATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTACATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCCGTGAAAACTCACGCGGTTCGATCt                                                                                                   >  3:42858/1‑275 (MQ=255)
                                                                                                                                                                                 aCGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGGTCATCACTTAACGAGGTGAAAACTCCAGCGGTTCGATCTTCCAccg                                                                                            >  2:14933/1‑273 (MQ=255)
                                                                                                                                                                                 aCGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATCCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTg                                                                                            <  2:119758/275‑1 (MQ=255)
                                                                                                                                                                                   gCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCa                                                                                                                                                                                                  >  1:217756/1‑171 (MQ=255)
                                                                                                                                                                                       gTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTAc                                                                                                                                                                                              <  3:217755/171‑1 (MQ=255)
                                                                                                                                                                                        tATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGAt                                                                                     >  1:157373/1‑275 (MQ=255)
                                                                                                                                                                                        tATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGAt                                                                                     >  1:66873/1‑275 (MQ=255)
                                                                                                                                                                                                 cacGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTc                                                                            <  1:176902/275‑1 (MQ=255)
                                                                                                                                                                                                                aaTCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGGTAAAACTCAAGCGGTTCGATCTTCAACTGCTCCGATTCAATCTTCGAGAAATCGAGAata                                                             >  1:259253/1‑275 (MQ=255)
                                                                                                                                                                                                                                       gaaaCTATGCAGCCGGTATCGGTGAAGTTTATGTCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCCTCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAg                                      <  2:235631/274‑1 (MQ=255)
                                                                                                                                                                                                                                             ataCAGCCGGTATCGGTGAATTTTATGGCGTTACTGACCAGGTTGGAGATGACCTGCGTTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTgg                                 <  1:63646/274‑1 (MQ=255)
                                                                                                                                                                                                                                             ataCAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTgg                                 <  3:66873/274‑1 (MQ=255)
                                                                                                                                                                                                                                             ataCAGCAGGTACTGGGGAATTTTATGGCGTTACGTGACCGGTTGGAGATGACCTGCTGTAAACGCAACGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTgg                                 <  2:266685/274‑1 (MQ=255)
                                                                                                                                                                                                                                              taCAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTCAATAGTTg                                                                                                                                                       >  1:89908/1‑155 (MQ=255)
                                                                                                                                                                                                                                                  gCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCgg                                                                                                                                                   <  3:89908/155‑1 (MQ=255)
                                                                                                                                                                                                                                                   ccGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTGCGATTCAATCTTCGAGTAATCGAGAATATCGCTGATAATTTTCTACAACAGGCTGGCAgtgt                           >  2:73418/1‑274 (MQ=255)
                                                                                                                                                                                                                                                               aaTTTTTTGGCGTTACTGAACAGGTGGGGGAGGACCTGGGTAAAAAGAAGCGGGTCGCCAATTAAGGCCACCGGCACAGCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGt              <  3:259252/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                         cGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCACCAGCCGa    >  2:215609/1‑275 (MQ=255)
                                                                                                                                                                                                                                                                           ttACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGTTGGAAGAGTTGTTCATTACCGTCACCAGCCGacc  >  4:215333/1‑273 (MQ=255)
                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                             
GTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCACCAGCCGATC  >  NC_000913/2317823‑2318364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: