Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,535,976 G→T G48V (GGT→GTT)  crr → glucose‑specific enzyme IIA component of PTS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,535,9760GT98.9% 291.7 / NA 91G48V (GGT→GTT) crrglucose‑specific enzyme IIA component of PTS
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (48/42);  total (48/43)
Fisher's exact test for biased strand distribution p-value = 4.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

CCGACAACGGACGAGTTAATGACGCTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGAC  >  NC_000913/2535731‑2536243
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                           
ccGACAACGGACGCATTAAGCACGCTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGGTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGt                                                                                                                                                                                                                                                 <  4:49774/274‑1 (MQ=255)
ccGACAACGGACGAGTTAATGACGCTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGt                                                                                                                                                                                                                                                 <  1:103556/274‑1 (MQ=255)
acGAAAAAGGGCGAGTTAATGAGGCTGGTTAACAAGTTCATTGAAAAAAAAACAATCTGCTAGTGAACGAGATGCGGCCCAATTTACTGCTGAGGAGAAGATCATGTGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGt                                                                                                                                                                                                                                                 <  2:256407/273‑1 (MQ=255)
  gACAACGGACGAGTTAATGACGCTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGGTTCCGACGACAAGAAGGATACCGGAACTATTAAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCTAAGACGTGCCGGATGTCGGTTTTGCGGACAAAATCGTTGTTAGTGGTTTTGCTATCAAAACAACGGGTAAc                                                                                                                                                                                                                                              >  4:94040/1‑275 (MQ=255)
     aaCGGACGAGTTAATGACGCTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACaaa                                                                                                                                                                                                                                           >  3:91372/1‑275 (MQ=255)
            gggTTAATGACTCTGGTTAACAAGTGCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTcg                                                                                                                                                                                                                                    <  2:235206/273‑1 (MQ=255)
            gatttAATGACGCTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAAACCGCGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTAGATAAACTTAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTcg                                                                                                                                                                                                                                    <  3:184897/272‑1 (MQ=255)
               ttAATATCGCAGGTTAACAACTTCAGTGAAAAAAAAACAATCTGCGAATCCACGAGATGCGGCCCAATTTACTGCTTATGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCgcgc                                                                                                                                                                                                                                 <  2:104795/275‑1 (MQ=255)
                 aaTGACGCAGGTTAACAAGTTCATTGAAGAAAAAACAATCGGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCa                                                                                                                                                                                                                               <  2:1540/275‑1 (MQ=255)
                   tGACGCTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGCAGACGTGCCGGATGTCGTTTTTGAGGAAAAAAACGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGt                                                                                                                                                                                                                             >  1:71580/1‑275 (MQ=255)
                        cTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCTGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACg                                                                                                                                                                                                                        >  1:259929/1‑275 (MQ=255)
                        cTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTg                                                                                                                                                                                                                                                                  >  1:80361/1‑233 (MQ=255)
                        cTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTATGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCTCTCTCTGGCGAGATCGCCAATATCGAAGCCGTGCCGGATGCAGTTTTTGCGGAACAACTGTTTGTTGATGGTATTGCTATCCAACCAACGGGTACCAAAAAGGTTGTCGCAGTAGACg                                                                                                                                                                                                                        >  3:11662/1‑275 (MQ=255)
                          ggTAAACAATTTACTTTAAGAAAAAACAAGTTGCTACTCCAAGAGCTGCGGCCCAATTTAATGCTTAGGAGAAGATCATGGGGTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGANGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGc                                                                                                                                                                                                                      <  4:136741/275‑1 (MQ=255)
                            ttAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTAt                                                                                                                                                                                                                                                              <  3:80361/233‑1 (MQ=255)
                              aagtaGTTCATTTAAGAAAAAACAATCCACTAATCCACAGGAGTCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCa                                                                                                                                                                                                                  <  1:129309/271‑1 (MQ=255)
                                     tCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATAATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGCCGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGGCACAAAATGGTCGCACCCGTCGACCGCACACTTGGTa                                                                                                                                                                                                            >  2:136741/1‑274 (MQ=255)
                                        ttGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTCCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAAGGGTCGCGCCAAGACCCGGCACCATTGGTAAAAt                                                                                                                                                                                                        >  3:64913/1‑275 (MQ=255)
                                          gaagaaAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATGTCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCCACGGGTAACAAAATGGTCGCGCCAGTAGAAGGCACCATTGGGAAAATCt                                                                                                                                                                                                      >  4:124589/1‑275 (MQ=255)
                                             gaaAAAACAAGCTGCTAATCCACGAGATGCGGCCCAATGTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATTTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTg                                                                                                                                                                                                   <  2:248313/275‑1 (MQ=255)
                                              aaaaaaCAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTGGCGGAAAAAATCGTTGTTTATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCTCGCCAGTAGAAGGGACCATTGGTAAAAACtttta                                                                                                                                                                                                  >  2:209192/1‑273 (MQ=255)
                                               aaaaaCAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGaa                                                                                                                                                                                                 <  2:53325/275‑1 (MQ=255)
                                                        cTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAAATGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCAGCTCTCTGGCGAGATAGTCAATATCGAAGACTTGCAGGATGTCGTTTGTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACACTTGGTCAAATCTTTGAACCCAACCAc                                                                                                                                                                                        >  2:246939/1‑275 (MQ=255)
                                                             aaTCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGTGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCAtt                                                                                                                                                                                   <  1:165895/275‑1 (MQ=255)
                                                             aaTCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCAtt                                                                                                                                                                                   <  3:255940/275‑1 (MQ=255)
                                                              aTCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATtc                                                                                                                                                                                  >  4:181501/1‑275 (MQ=255)
                                                              aTCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGACGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATTTTTGAAACCAACCACGAATtc                                                                                                                                                                                  >  3:110517/1‑275 (MQ=255)
                                                                 cACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGCACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCGATATCGAAGACGTGCCGGCTGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTTGCGCCAGTGGACGGCACCATTGGTAAAATCTTTGAAACAAACATCGCATtcgc                                                                                                                                                                                >  4:240618/1‑272 (MQ=255)
                                                                  aCGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTa                                                                                                                                                                              <  1:168633/275‑1 (MQ=255)
                                                                  aCGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTa                                                                                                                                                                              <  1:65119/275‑1 (MQ=255)
                                                                   cGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGTGAAAAGCTTTGAAAACAACCACCCATTCCCTAt                                                                                                                                                                             >  3:257206/1‑275 (MQ=255)
                                                                     agaTGAGGCCCAATTTACTTGTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGAAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCg                                                                                                                                                                           <  2:170101/275‑1 (MQ=255)
                                                                         gCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCTGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCACTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTATCTATCGAATc                                                                                                                                                                       >  2:203269/1‑275 (MQ=255)
                                                                               cAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAg                                                                                                                                                                 <  2:181501/275‑1 (MQ=255)
                                                                                     aCTGCTTAGGGGAAGTTCATGGGTTTTTGCGATAAACTTAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAgcggcg                                                                                                                                                            <  4:209192/274‑1 (MQ=255)
                                                                                       tGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGGTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGGATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAAGCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGt                                                                                                                                                           >  1:135698/1‑273 (MQ=255)
                                                                                                 aagaTCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCTCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGt                                                                                                                                               <  1:116773/275‑1 (MQ=255)
                                                                                                   gaTCATGGGTTTGTTCGTTACACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCAGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGACAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCGAGAATTCTCTATCGAATCTGATCGCGGCGTTGAACttgtcgacc                                                                                                                                             >  2:103594/1‑267 (MQ=255)
                                                                                                   gaTCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTTTCAAACCAACGGGTAACACAATGGTCGCGTCAGTAGCCGGAACCATTGGTAAAATCTTTGAGACCAACCACGCCTTCCCTATTCAATATGATAGCGGAGTTCAACTGTTTGTca                                                                                                                                             >  3:144495/1‑274 (MQ=255)
                                                                                                   gaTCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGTCACAAAATGGTTGCGACAGTAGACGGCACCATTGGAAAAATCGTTGAACCCAACACGGCATTCTATATCGAATCTGATAGCGTCTTTAACATGTTCGTca                                                                                                                                             >  1:35867/1‑274 (MQ=255)
                                                                                                   gaTCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTg                                                                                                                                                                     >  4:32292/1‑251 (MQ=255)
                                                                                                   gaTCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAAACAACCACGCCTTCTCTATCGACTCTGAAAGCGGCTTTGAAATGTTCGtac                                                                                                                                             >  4:237389/1‑273 (MQ=255)
                                                                                                       aTGGGTTTTTTCGATAAACTGAAATCTCTGGTTTCCGACGAAAAGAAGGATACCGGAACTATTGAGATCATTGCTACGCTCTCAGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACt                                                                                                                                          <  2:73046/274‑1 (MQ=255)
                                                                                                       aTGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAg                                                                                                                                                                 <  2:32292/251‑1 (MQ=255)
                                                                                                        tGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACTGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCCCTATCGAATCTGATAGCGGCATTGAACTTTTCGTCCACTTc                                                                                                                                        >  2:210679/1‑275 (MQ=255)
                                                                                                              tGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCCTTGGTAAAATCTTTGAAACCAACCACGCATTCCCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGt                                                                                                                                     >  3:155083/1‑272 (MQ=255)
                                                                                                                ttCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGAACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTACTGAATTTGATAGCGGCTTTGAACTGTTCGTCCATTTCGGTATCGa                                                                                                                                >  4:107687/1‑275 (MQ=255)
                                                                                                                                   tGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCCATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGACCTGAAAGGc                                                                                                             >  3:266332/1‑275 (MQ=255)
                                                                                                                                    ggTTGCCGACGAAAAGAAGGATACCGGAACAATTGAGATCATTGCGCCTCTCTCTGGCGAGATCTTAAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCg                                                                                                            <  4:246939/275‑1 (MQ=255)
                                                                                                                                         ccGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGc                                                                                                       <  1:91372/275‑1 (MQ=255)
                                                                                                                                            acgacAAGAAGGATACCGGAACGATTGAGATCATTGCTCCGCTTTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATAAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTc                                                                                                    <  1:137457/275‑1 (MQ=255)
                                                                                                                                                 aagaagGATAACGGAACTATTGGGATCATTGCTCCGCTCTCTGGCGAGATCGTGAATATAGAAAACGTGCCGGATGTCGTTTTTGCGGGAAAAATCGTTGTGGGTGGTATTGCTATCAAACAAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAAGCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCg                                                                                               <  1:219013/275‑1 (MQ=255)
                                                                                                                                                    aagGATACCGGAACTATTGAGGTCATTGCTCCGCTCTCTTGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTa                                                                                             <  1:1733/274‑1 (MQ=255)
                                                                                                                                                       gttACCTGAACTATTGAGCTCATTGCTCCTCTCTCTGGCGAGATCGTCAAAATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCACTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGc                                                                                         <  4:203269/273‑1 (MQ=255)
                                                                                                                                                            cggacgtATTGGGTTGATTGAGCCGGTATCTGGCTAGACTGTCAATATGGAAGACGTGCCGGATTGTGATTTTGCCGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAAAGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCCACCACGCATTCTCTNTCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTgaag                                                                                    <  4:150580/269‑1 (MQ=255)
                                                                                                                                                              gAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCTACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGCATCGACACCGTTGAACTGAAAGGAGAAGGCTTCAAGCGTATTGTTgaagaa                                                                                  >  3:242563/1‑275 (MQ=255)
                                                                                                                                                              gAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGCACTGTTGGTCTACTTCGGTAGCTCAACCGTTGAACTGAACGGCGACGGCTGCAAGGGGATTGCTgtagaa                                                                                  >  4:243559/1‑275 (MQ=255)
                                                                                                                                                              gAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTa                                                                                                                                    >  2:27368/1‑225 (MQ=255)
                                                                                                                                                              gAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTgaagaa                                                                                  >  4:214489/1‑275 (MQ=255)
                                                                                                                                                              gAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTgaagaa                                                                                  >  2:120448/1‑275 (MQ=255)
                                                                                                                                                                  tATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGa                                                                                                                                <  4:27368/225‑1 (MQ=255)
                                                                                                                                                                         aTCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGcgcg                                                                        >  1:262833/1‑274 (MQ=255)
                                                                                                                                                                         aTCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGcgcg                                                                        >  3:7112/1‑274 (MQ=255)
                                                                                                                                                                         aTCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAACCGTATTGCGGACGAGGGTCAGCGCGt                                                                       >  3:128896/1‑275 (MQ=255)
                                                                                                                                                                         aTCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCAAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTACCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACAGTTTAACTGAAAGGCGAGGGCTTCAAGCGTATTTATGACGAAAGTAAGCTCGt                                                                       >  2:26965/1‑275 (MQ=255)
                                                                                                                                                                            aTTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCTACACCGTTGAACTGAAAGGCGACGGCTTCAAGCGTATTGCTGAAGAAGGTCAACGCGttag                                                                    >  4:266587/1‑272 (MQ=255)
                                                                                                                                                                              tGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCCATGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTCTTCGTCCACTTCCGTATCTACACCGTTTACCTGAAAGGCGACGGCTTCAAGCGTATTTATGAAGAAGGTCAGCGCATGAAAg                                                                  >  2:98500/1‑275 (MQ=255)
                                                                                                                                                                              tGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGCTAGCGGCGTTGAACTGTTCTTCCACTTCGGTATCGACACCGTTGACCTGAAAGGCGAAGGCTTCAATCGTATTGATGCAGAAGGTCACCGCGCGAAAg                                                                  >  3:210529/1‑275 (MQ=255)
                                                                                                                                                                               gttCCGCTCTCTGGGGAGATCGTCTATATCTAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACAACGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGt                                                                 <  2:215643/273‑1 (MQ=255)
                                                                                                                                                                                   cGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAAATGAAAGGCGAAGGCTTCa                                                                                                   >  4:214386/1‑237 (MQ=255)
                                                                                                                                                                                     ctctctGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTCGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGCATAAGGTCAGCGCGTGAAAGTTGgaga                                                           >  3:81015/1‑275 (MQ=255)
                                                                                                                                                                                     ctctctGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCCAATCTGATAGCGGCGTTTAACTGTTAGTCCACTGCGGTACCGACACCGTTGAACTGAAAGGCGAAGGCTTCACGCGTATTACTGAAGCAGGTCAGGGCGGGAAAGTTGGCGa                                                           >  1:71216/1‑275 (MQ=255)
                                                                                                                                                                                       ctctGGCGAGATCGTTAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGATATTGCTATCAAACAAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACTACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTTTTCGTCTACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCg                                                                                               <  2:214386/237‑1 (MQ=255)
                                                                                                                                                                                        tctTGCGAGATCGTCAATATCGAAGACGTGCCTGATGTCGTTTTTGCAGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATa                                                         <  3:259928/274‑1 (MQ=255)
                                                                                                                                                                                        tctGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGaa                                                                                                                                                       >  1:38513/1‑180 (MQ=255)
                                                                                                                                                                                          tGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAANAAAATGGTCGCGCCAGTAGACGGCACCATTGGTACAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACCCGGTTCAACTGAAAGGCGAAGGCTCAAAGCGTATTCCTTGACAAGGGCAGCGCGTGAAAGTCGGGGATAcgg                                                      >  4:145960/1‑273 (MQ=255)
                                                                                                                                                                                            gCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGt                                                                                                                                                   <  3:38513/180‑1 (MQ=255)
                                                                                                                                                                                                            cGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAATTCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACTGCACCATTCGTAAAATCTTTGAAACCAACCACGCATTCTCTCTCGAATCTGATAGCGCCGTTTAACTGTTCGTCTACTTCCGTATCGACACCGTTCAACTGAAAGGCGAAGGTTTCAAGCGCATTGCTGAAGAAGGTCAGCGCTGGAAAGTTGGCTCTACTGTCATTGACTTTTAACTGc                                    >  3:197680/1‑275 (MQ=255)
                                                                                                                                                                                                                   gTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTAAAGCGTATTGCTGAATAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCgctgct                             >  4:84321/1‑275 (MQ=255)
                                                                                                                                                                                                                    tGCCGGATGTCGTTTTTGCGGAAAAAAGCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGctgctg                            <  3:135697/275‑1 (MQ=255)
                                                                                                                                                                                                                     gCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATCCTGTCATTGAATTTGATCTGCCGCTGCTgg                           >  3:217172/1‑275 (MQ=255)
                                                                                                                                                                                                                     gCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAAGCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGATGCACTGAAAGGCGAAGGCTTCAACCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACGGTCATTTAATTTGATCTGACGCTGCTgg                           >  1:227897/1‑275 (MQ=255)
                                                                                                                                                                                                                        ggATGTCGGTTTGGCGGAAAAAATCGTTGTTGATGGTAATGCTATCAAAACAAAGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAag                        <  4:120448/275‑1 (MQ=255)
                                                                                                                                                                                                                             tCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGaaa                   <  1:7112/275‑1 (MQ=255)
                                                                                                                                                                                                                               gTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAg                  >  3:231076/1‑274 (MQ=255)
                                                                                                                                                                                                                               gTTTGTGCGGAAAAAATCGTTGTTTATGGTATTGCTCACAAACCAACGGGTAACAAGATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGc                 <  2:124589/275‑1 (MQ=255)
                                                                                                                                                                                                                               gTTTATGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACAAACGGGTAACAGAATGGTCGAGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGc                 <  1:110517/275‑1 (MQ=255)
                                                                                                                                                                                                                                tttttGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGc                 <  1:155084/274‑1 (MQ=255)
                                                                                                                                                                                                                                     ggggAAAGAATCGTTGTTGAGTATATTGCTATAAAACCAAGGGGTAACAAAATGGTCGCGCCAGTAGACGGCAACATTGGTAAACACTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGt            <  1:64913/272‑1 (MQ=255)
                                                                                                                                                                                                                                               tCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTATACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGAc  <  1:173577/274‑1 (MQ=255)
                                                                                                                                                                                                                                               tCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCTGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGAc  <  2:266587/274‑1 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                           
CCGACAACGGACGAGTTAATGACGCTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGAC  >  NC_000913/2535731‑2536243

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: