Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 1,799,374:1 | +T | 5.4% | intergenic (‑104/+19) | pheM ← / ← rplT | phenylalanyl‑tRNA synthetase operon leader peptide/50S ribosomal subunit protein L20 |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,799,374 | 1 | . | T | 5.4% | 465.2 / 21.0 | 111 | intergenic (‑104/+19) | pheM/rplT | phenylalanyl‑tRNA synthetase operon leader peptide/50S ribosomal subunit protein L20 |
Reads supporting (aligned to +/- strand): ref base . (54/51); new base T (2/4); total (56/55) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 4.38e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 |
Only 100 of 112 total aligned reads displayed.
CAGGTGCTAAAGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCTTTTTTCAGGCCATTGATGAATTT > NC_000913/1799227‑1799518 | gCTAAAGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAG‑CTAGTCTCCct < 4:862958/149‑1 (MQ=255) cTAAAGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAG‑CTAGTCTCCctc < 3:391844/149‑1 (MQ=255) tAAAGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCctct > 4:382363/1‑149 (MQ=255) tAAAGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAG‑CTAGTCTCCctct < 4:123345/149‑1 (MQ=255) aaaGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCctct > 1:249584/1‑148 (MQ=255) aaaGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAG‑CTAGTCTCCCTCtt < 3:944410/149‑1 (MQ=255) gaaaaaaGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAAc < 3:112849/148‑1 (MQ=255) aaaaaaaGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCttttcgt < 4:606618/149‑5 (MQ=255) aaaaaaGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCttt > 3:439593/1‑144 (MQ=255) aaaaaaGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCttt < 4:439593/144‑1 (MQ=255) aaaaaaGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACt > 3:150231/1‑149 (MQ=255) aaaaGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTg > 1:617058/1‑148 (MQ=255) aagaagCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGc < 2:488371/148‑1 (MQ=255) aagCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTa < 1:718008/148‑1 (MQ=255) agCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATg < 3:603418/149‑1 (MQ=255) cGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGc > 1:497681/1‑148 (MQ=255) ggAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGcc < 2:387401/148‑1 (MQ=255) ggAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCa < 3:838546/149‑1 (MQ=255) aaaaTAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCaga < 3:347957/149‑1 (MQ=255) tAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCATAAGCCagttga < 2:49203/148‑5 (MQ=255) gcaTTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCTCTCTTTCAACTGGCTTATGCCa > 3:535364/1‑138 (MQ=255) gcaTTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCTCTCTTTCAACTGGCTTATGCCa < 4:535364/138‑1 (MQ=255) gcaTTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTc > 3:792861/1‑149 (MQ=255) ttcattGCTTGCGTTACCTTTTGGTGCTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCt > 4:632911/1‑149 (MQ=255) ttcattGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCt < 3:629823/149‑1 (MQ=255) ttcattGCTGGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCt > 4:269575/1‑149 (MQ=255) cttgcGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCaa < 3:408075/149‑1 (MQ=255) cTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCaga < 4:294908/144‑1 (MQ=255) cTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGt < 3:696259/149‑1 (MQ=255) ttttGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCctc < 3:864012/98‑1 (MQ=255) ttttGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCctc > 4:864012/1‑98 (MQ=255) ttttGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGt > 2:102531/1‑148 (MQ=255) tttGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTg > 2:186616/1‑148 (MQ=255) tGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGaa < 1:23762/148‑1 (MQ=255) ctctTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTAc > 4:882382/1‑149 (MQ=255) ttCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACt < 4:535084/95‑1 (MQ=255) cAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTg < 2:643621/148‑1 (MQ=255) aaaaGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGt < 2:312184/148‑1 (MQ=255) aaaaGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGt < 1:657904/148‑1 (MQ=255) aaaaGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTc > 4:544873/1‑149 (MQ=255) aaaaGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTc > 3:52639/1‑149 (MQ=255) aaGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCaga > 3:137271/1‑103 (MQ=255) aaGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCGCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGTGGTGAACGCTACTTTGTCGa < 4:152473/149‑1 (MQ=255) gACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCttttt > 2:355974/1‑117 (MQ=255) gACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCttttt < 1:355974/117‑1 (MQ=255) ccTTTTCTGTAAAAGCTACGACGAGAAAAGTGAATGCGTTAATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTACTTTTTTCAACTGGCTTATGCCAGTGCTGCTTTCGCTTTTTCATCTAGAGCGGTGCAAGCTACTTTGTCGAATa > 2:543372/1‑148 (MQ=255) tttATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTg > 3:23767/1‑149 (MQ=255) aTTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGa < 4:823371/149‑1 (MQ=255) aaaaGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATc > 3:745080/1‑149 (MQ=255) aaaaGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATc > 4:812499/1‑149 (MQ=255) aaaaGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATc > 3:235755/1‑149 (MQ=255) ggCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGc > 2:494152/1‑148 (MQ=255) gCTACGGCGATATAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCa < 3:916707/149‑1 (MQ=255) cTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCa > 2:156813/1‑148 (MQ=255) aCGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGa > 4:365791/1‑149 (MQ=255) ggCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCg < 2:511667/134‑1 (MQ=255) ggCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCg > 1:511667/1‑134 (MQ=255) ggCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATc < 4:497580/149‑1 (MQ=255) aTAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCct > 2:765170/1‑54 (MQ=255) aTAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCct < 1:765170/54‑1 (MQ=255) aTAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCtt < 2:433968/89‑1 (MQ=255) aTAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCtt > 1:433968/1‑89 (MQ=255) tAAAAGTCAATGTTTTGATGGCGTTGAAAGGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTAc > 1:713604/1‑148 (MQ=255) aaaGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTACGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACgg > 1:914225/1‑148 (MQ=255) tCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGa > 1:417351/1‑148 (MQ=255) tCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGAt < 4:890906/149‑1 (MQ=255) aTGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGAttt > 1:889531/1‑148 (MQ=255) aTGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGAttt > 2:154750/1‑148 (MQ=255) tGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCa > 4:754592/1‑149 (MQ=255) gTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGTTTTCaa < 4:614694/149‑1 (MQ=255) gTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCaa < 3:382363/149‑1 (MQ=255) gTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCaa > 3:197752/1‑149 (MQ=255) ttttGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATACCAGCCAGGATCTTACGGTCGATTTCAAc < 3:895525/149‑1 (MQ=255) atttGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAAc > 3:537667/2‑149 (MQ=255) tttGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAAc > 1:810179/1‑148 (MQ=255) tttGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAAc > 2:84669/1‑148 (MQ=255) tGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGc > 3:577161/1‑149 (MQ=255) tgCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCGACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGc > 1:288046/2‑148 (MQ=255) gTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCtttt < 4:150231/149‑1 (MQ=255) gTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCtttt > 3:813548/1‑149 (MQ=255) aaaCGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCTTTTTTc < 2:249584/148‑1 (MQ=255) aaaCGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCTTTTTTc > 2:650307/1‑148 (MQ=255) aaCGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCaga < 4:17258/48‑1 (MQ=255) aaCGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCaga > 3:17258/1‑48 (MQ=255) aaCGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCTTTTTTCa < 1:186616/148‑1 (MQ=255) aaCGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCTTTTTTCa > 2:385101/1‑148 (MQ=255) aaCGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCTTTTTTCa < 1:385101/148‑1 (MQ=255) aCGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCTTTTTTCAgg > 3:302788/1‑149 (MQ=255) aaaGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCTTTTTTCAGGCCa > 2:588887/1‑148 (MQ=255) aaaGAGGGAG‑CTAGTCTCCCTCTTTTAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCTTTTTTCAGGCCa > 1:572088/1‑148 (MQ=255) aaGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCTTTTTTCAGGCCAt > 2:628130/1‑148 (MQ=255) aaGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCTTTTTTCAGGCCAt < 1:588887/148‑1 (MQ=255) agagGGAG‑CTAGTCTCCCTCTTTCAACTGGATTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCTTTTTTCAGGCCATtg > 4:231144/1‑149 (MQ=255) gagGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGCGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCTTTTTTCAGGCCATtga < 3:629231/149‑1 (MQ=255) gagGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTg < 2:750792/70‑1 (MQ=255) agGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCTTTTTTCAGGCCATtgat < 3:34273/149‑1 (MQ=255) gggAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGTGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCTTTTTTCAGGCCATtgatg < 4:52639/149‑1 (MQ=255) cTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCTTTTTTCAGGCCATTGATGAAttt < 1:127520/148‑1 (MQ=255) tAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCTTTTTTCAGGCCATTGATGAAt < 4:487740/145‑1 (MQ=255) tAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCTTTTTTCAGGCCATTGATGAAt > 3:487740/1‑145 (MQ=255) | CAGGTGCTAAAGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAG‑CTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCTTTTTTCAGGCCATTGATGAATTT > NC_000913/1799227‑1799518 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |