Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 977,479 C→T 5.1% A385V (GCG→GTG)  mukB → chromosome condensin MukBEF, ATPase and DNA‑binding subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913977,4790CT 5.1% 260.2 / 2.6 78A385V (GCG→GTG) mukBchromosome condensin MukBEF, ATPase and DNA‑binding subunit
Reads supporting (aligned to +/- strand):  ref base C (33/41);  new base T (2/2);  total (35/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

CACCTGAACCTGGTGCAAACCGCACTGCGTCAGCAGGAGAAGATCGAACGCTACGAAGCGGATCTCGATGAGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCAGCTTGCTGACTACCAGCAGGCGCTGGACGTCCAGCAAACGCGCGCGATCCAGTATAACCAGGCGATTGCTGCGCTTAATCGTGCCAAAGAACTGTGCCATCTGCCGGAC  >  NC_000913/977331‑977627
                                                                                                                                                    |                                                                                                                                                    
cACCTGAACCTGGTGCAAACCGCACTGCGTCAGCAGGAGAAGATCGAACGCTACGAAGCGGATCTCGATGAGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGc                                                                                                                                                      <  4:871526/149‑1 (MQ=255)
cACCTGAACCTGGTGCAAACCGCACTGCGTCAGCAGGAGAAGATCGAACGCTACGAAGCGGATCTCGATGAGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGc                                                                                                                                                      >  3:377025/1‑149 (MQ=255)
    tGAACCTGGTGCAAACCGCACTGCGTCAGCAGGAGAAGATCGAACGCTACGAAGCGGATCTCGATGAGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGTCAGCAAGAGAATGAGGCTCGTTCAGaa                                                                                                                                                  >  4:825316/1‑149 (MQ=255)
    tGAACCTGGTGCAAACCGCACTGCGTCAGCAGGAGAAGATCGAACGCTACGAAGCGGATCTCGATGAGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGa                                                                                                                                                   <  2:724944/148‑1 (MQ=255)
            gTGCAAACCGCACTGCGTCAGCAGGAGAAGATCGAACGCTACGAAGCGGATCTCGATGAGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCg                                                                                                                                           >  2:274251/1‑148 (MQ=255)
              gCAAACCGCACTGCGTCAGCAGGAGAAGATCGAACGCTACGAAGCGGATCTCGATGAGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAAc                                                                                                                                        <  4:367848/149‑1 (MQ=255)
              gCAAACCGCACTGCGTCAGCAGGAGAAGATCGAACGCTACGAAGCGGATCTCGATGAGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAAc                                                                                                                                        >  3:715319/1‑149 (MQ=255)
              gCAAACCGCACTGCGTCAGCAGGAGAAGATCGAACGCTACGAAGCGGATCTCGATGAGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAAc                                                                                                                                        <  3:251692/149‑1 (MQ=255)
               cAAACCGCACTGCGTCAGCAGGAGAAGATCGAACGCTACGAAGCGGATCTCGATGAGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAAc                                                                                                                                        <  1:877004/148‑1 (MQ=255)
               cAAACCGCACTGCGTCAGCAGGAGAAGATCGAACGCTACGAAGCGGATCTCGATGAGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACt                                                                                                                                       <  4:377025/149‑1 (MQ=255)
                       aCTGCGTCAGCAGGAGAAGATCGAACGCTACGAAGCGGATCTCGATGAGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTgg                                                                                                                               >  3:911947/1‑149 (MQ=255)
                        cTGCGTCAGCAGGAGAAGATCGAACGCTACGAAGCGGATCTCGATGAGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGa                                                                                                                              >  3:876879/1‑149 (MQ=255)
                         tGCGTCAGCAGGAGAAGATCGAACGCTACGAAGCGGATCTCGATGAGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGa                                                                                                                              <  2:585767/148‑1 (MQ=255)
                         tGCGTCAGCAGGAGAAGATCGAACGCTACGAAGCGGATCTCGATGAGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGAc                                                                                                                             <  3:45575/149‑1 (MQ=255)
                           cGTCAGCAGGAGAAGATCGAACGCTACGAAGCGGATCTCGATGAGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACg                                                                                                                            <  2:767783/148‑1 (MQ=255)
                             tCAGCAGGAGAAGATCGAACGCTACGAAGCGGATCTCGATGAGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAg                                                                                                                          <  1:893333/148‑1 (MQ=255)
                             tCAGCAGGAGAAGATCGAACGCTACGAAGCGGATCTCGATGAGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAg                                                                                                                          <  1:631973/148‑1 (MQ=255)
                                gcagGAGAAGATCGAACGCTACGAAGCGGATCTCGATGAGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTg                                                                                                                       >  1:330458/1‑148 (MQ=255)
                                   ggAGAAGATCGAACGCTACGAAGCGGATCTCGATGAGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGaaaa                                                                                                                   <  4:61001/149‑1 (MQ=255)
                                   ggAGAAGATCGAACGCTACGAAGCGGATCTCGATGAGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGaaa                                                                                                                    >  1:836123/1‑148 (MQ=255)
                                      gaagaTCGAACGCTACGAAGCGGATCTCGATGAGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGc                                                                                                                 <  1:968628/148‑1 (MQ=255)
                                       aagaTCGAACGCTACGAAGCGGATCTCGATGAGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGcc                                                                                                                <  1:433692/148‑1 (MQ=255)
                                       aagaTCGAACGCTACGAAGCGGATCTCGATGAGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCa                                                                                                               <  3:493176/149‑1 (MQ=255)
                                       aagaTCGAACGCTACGAAGCGGATCTCGATGAGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCa                                                                                                               <  4:32135/149‑1 (MQ=255)
                                          aTCGAACGCTACGAAGCGGATCTCGATGAGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCAGc                                                                                                             >  2:769622/1‑148 (MQ=255)
                                             gAACGCTACGAAGCGGATCTCGATGAGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCAGCTTg                                                                                                          <  1:529148/148‑1 (MQ=255)
                                             gAACGCTACGAAGCGGATCTCGATGAGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCAGCTTGc                                                                                                         <  4:254469/149‑1 (MQ=255)
                                               aCGCTACGAAGCGGATCTCGATGAGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCAGCTTGCt                                                                                                        >  2:454343/1‑148 (MQ=255)
                                                cGCTACGAAGCGGATCTCGATGAGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCAGCTTGCTGa                                                                                                      <  4:495508/149‑1 (MQ=255)
                                                  cTACGAAGCGGATCTCGATGAGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCAGCTTGCTGAc                                                                                                     >  1:881263/1‑148 (MQ=255)
                                                    aCGAAGCGGATCTCGATGAGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCAGCTTGCTGACTa                                                                                                   <  2:881263/148‑1 (MQ=255)
                                                      gAAGCGGATCTCGATGAGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCAGCTTGCTGACTACca                                                                                                <  3:858129/149‑1 (MQ=255)
                                                       aaGCGGATCTCGATGAGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCAGCTTGCTGACTACca                                                                                                >  1:925918/1‑148 (MQ=255)
                                                        aGCGGATCTCGATGAGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCAGCTTGTTGACTACcagc                                                                                              <  3:825316/149‑1 (MQ=255)
                                                             aTCTCGATGAGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGTACTGGAAGTGGACGAGCTGAAAAGCCAGCTTGCTGACTACCAGCAGgcg                                                                                         >  4:72937/1‑149 (MQ=255)
                                                                   aTGAGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCAGCTTGCTGACTACCAGCAGGCGCTGGAc                                                                                   >  4:663596/1‑149 (MQ=255)
                                                                      aGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGCGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCAGCTTGCTGACTACCAGCAGGCGCTGGACGt                                                                                 <  2:690790/148‑1 (MQ=255)
                                                                      aGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCAGCTTGCTGACTACCAGCAGGCGCTGGACGt                                                                                 >  2:181485/1‑148 (MQ=255)
                                                                      aGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCAGCTTGCTGACTACCAGCAGGCGCTGGACGTc                                                                                <  3:183998/149‑1 (MQ=255)
                                                                        cTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGc                                                                                                                         <  4:244849/106‑1 (MQ=255)
                                                                        cTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGc                                                                                                                         >  3:244849/1‑106 (MQ=255)
                                                                        cTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCAGCTTGCTGACTACCAGCAGGCGCTGGACGTcc                                                                               <  2:680655/148‑1 (MQ=255)
                                                                          gCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCAGCTTGCTGACTACCAGCAGGCGCTGGACGTCCAGc                                                                            >  4:445755/1‑149 (MQ=255)
                                                                              aTCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGTGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAGAGCCAGCTTGCTGACTACCAGCAgg                                                                                           >  4:628726/1‑130 (MQ=255)
                                                                              aTCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGTGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCAGCTTGCTGACTACCAGCAgg                                                                                           >  4:719489/1‑130 (MQ=255)
                                                                              aTCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGTGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCAGCTTGCTGACTACCAGCAgg                                                                                           <  3:628726/130‑1 (MQ=255)
                                                                              aTCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGTGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCAGCTTGCTGACTACCAGCAgg                                                                                           <  3:719489/130‑1 (MQ=255)
                                                                                        aaGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCAGCTTGCTGACTACCAGCAGGCGCTGGACGTCCAGCAAACGCGCGCGAt                                                               <  2:925918/148‑1 (MQ=255)
                                                                                        aaGACCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGTATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGATGAAAAGCCAGCTTGCTGACTACCAGCAGGCGCTGGACGTCCAGCAAACGCGCGCGATc                                                              <  4:803444/149‑1 (MQ=255)
                                                                                                aaTGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCAGCTTGCTGACTACCAGCAGGCGCTGGACGTCCAGCAAACGCGCGCGATCCAGtata                                                       >  1:307345/1‑148 (MQ=255)
                                                                                                aaTGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCAGCTTGCTGACTACCAGCAGGCGCTGGACGTCCAGCAAACGCGCGCGATCCAGtata                                                       >  1:257694/1‑148 (MQ=255)
                                                                                                aaTGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCAGCTTGCTGACTACCAGCAGGCGCTGGACGTCCAGCAAACGCGCGCGATCCAGTATaa                                                      >  4:682655/1‑149 (MQ=255)
                                                                                                   gAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCAGCTTGCTGACTACCAGCAGGCGCTGGACGTcc                                                                               <  1:506919/121‑1 (MQ=255)
                                                                                                   gAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCAGCTTGCTGACTACCAGCAGGCGCTGGACGTcc                                                                               >  2:506919/1‑121 (MQ=255)
                                                                                                      gtggtgGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCAGCTTGCTGACTACCAGCAGGCGCTGGACGTCCAGCAAACGCGCGCGATCCAGTATAACCAGGc                                                <  4:565073/149‑1 (MQ=255)
                                                                                                        ggtggCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCAGCTTGCTGACTACCAGCAGGCGCTGGACGTCCAGCAAACGCGCGCGATCCAGTATAACCAGGCg                                               >  2:821006/1‑148 (MQ=255)
                                                                                                         gtggCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCAGCTTGCTGACTACCAGCAGGCGCTGGACGTCCAGCAAACGCGCGCGATCCAGTATAACCAGGCGa                                              <  2:836123/148‑1 (MQ=255)
                                                                                                         gtggCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCAGCTTGCTGACTACCAGCAGGCGCTGGACGTCCAGCAAACGCGCGCGATCCAGTATAACCAGGCGAt                                             <  3:574377/149‑1 (MQ=255)
                                                                                                         gtggCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCAGCTTGCTGACTACCAGCAGGCGCTGGACGTCCAGCAAACGCGCGCGATCCAGTATAACCAGGCGAt                                             >  4:807950/1‑149 (MQ=255)
                                                                                                             cAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAg                                                                                                                                  >  2:803919/1‑60 (MQ=255)
                                                                                                             cAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAg                                                                                                                                  <  1:803919/60‑1 (MQ=255)
                                                                                                             cAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCAGCTTGCTGACTACCAGCAGGCGCTGGACGTCCAGCAAACGCGCGCGATCCAGTATAACCAGGCGATtgct                                         <  3:834367/149‑1 (MQ=255)
                                                                                                              aGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCAGCTTGCTGACTACCAGCAGGCGCTGGACGTCCAGCAAACGCGCGCGATCCAGTATAACCAGGCGATtgctg                                        <  3:733166/149‑1 (MQ=255)
                                                                                                               gAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCAGCTTGCTGACTACCAGCAGGCGCTGGACGTCCAGCAAACGCGCGCGATCCAGTATAACCAGGCGATtgctgc                                       >  3:446183/1‑149 (MQ=255)
                                                                                                                aaGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCAGCTTGCTGACTACCAGCAGGCGCTGGACGTCCAGCAAACGCGCGCGATCCAGTATAACCAGGCGATtgctgc                                       >  1:192044/1‑148 (MQ=255)
                                                                                                                aaGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCAGCTTGCTGACTACCAGCAGGCGCTGGACGTCCAGCAAACGCGCGCGATCCAGTATAACCAGGCGATTGCTgcg                                      >  3:923799/1‑149 (MQ=255)
                                                                                                                  gCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCAGCTTGCTGACTACCAGCAGGCGCTGGACGTCCAGCAAACGCGCGCGATCCAGTATAACCAGGCGATTGCTGCGCt                                    >  3:168567/1‑149 (MQ=255)
                                                                                                                     aTCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCAGCTTGCTGACTACCAGCAGGCGCTGGACGTCCAGCAAACGCGCGCGATCCAGTATAACCAGGCGATTGCTGCGCTTa                                  >  2:292139/1‑148 (MQ=255)
                                                                                                                      tCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCAGCTTGCTGACTACCAGCAGGCGCTGGACGTCCAGCAAACGCGCGCGATCCAGTATAACCAGGCGATTGCTGCGCTTAAt                                >  3:215258/1‑149 (MQ=255)
                                                                                                                        gAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCAGCTTGCTGACTACCAGCAGGCGCTGGACGTCCAGCAAACGCGCGCGATCCAGTATAACCAGGCGATTGCTGCGCTTAATCg                              >  3:272816/1‑149 (MQ=255)
                                                                                                                         aaCGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCAGCTTGCTGACTACCAGCAGGCGCTGGACGTCCAGCAAACGCGCGCGATCCAGTATAACCAGGCGATTGCTGCGCTTAATCg                              <  1:274251/148‑1 (MQ=255)
                                                                                                                         aaCGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCAGCTTGCTGACTACCAGCAGGCGCTGGACGTCCAGCAAACGCGCGCGATCCAGTATAACCAGGCGATTGCTGCGCTTAATCGt                             >  3:124608/1‑149 (MQ=255)
                                                                                                                           cGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCAGCTTGCTGACTACCAGCAGGCGCTGGACGTCCAGCAAACGCGCGCGATCCAGTATAACCAGGCGATTGCTGCGCTTAATCGTg                            <  1:855908/148‑1 (MQ=255)
                                                                                                                           cGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCAGCTTGCTGACTACCAGCAGGCGCTGGACGTCCAGCAAACGCGCGCGATCCAGTATAACCAGGCGATTGCTGCGCTTAATCGTGc                           <  4:272816/149‑1 (MQ=255)
                                                                                                                                 cAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCAGCTTGCTGACTACCAGCAGGCGCTGGACGTCCAGCAAACGCGCGCGATCCAGTATAACCAGGCGATTGCTGCGCTTAATCGTGCCAAAg                      >  1:200302/1‑148 (MQ=255)
                                                                                                                                   agagAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCAGCTTGCTGACTACCAGCAGGCGCTGGACGTCCAGCAAACGCGCGCGATCCAGTATAACCAGGCGATTGCTGCGCTTAATCGTGCCAAAGaa                    <  1:82972/148‑1 (MQ=255)
                                                                                                                                    gagaATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCAGCTTGCTGACTACCAGCAGGCGCTGGACGTCCAGCAAACGCGCGCGATCCAGTATAACCAGGCGATTGCTGCGCTTAATCGTGCCAAAGAAc                   >  1:172759/1‑148 (MQ=255)
                                                                                                                                      gaATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCAGCTTGCTGACTACCAGCAGGCGCTGGACGTCCAGCAAACGCGCGCGATCCAGTATAACCAGGCGATTGCTGCGCTTAATCGTGCCAAAGAACtg                 <  1:292139/148‑1 (MQ=255)
                                                                                                                                        aTGAGGCTCGTGCGGAAGCTGCCGAGCTGGAAGTGGACGAGCTGAAAAGCCAGCTTGCTGACTACCAGCAGGCGCTGGACGTCCAGCAAACGCGCGCGATCCAGTATAACCAGGCGATTGCTGCGCTTAATCGTGCCAAAGAACtgtg               <  1:821006/148‑1 (MQ=255)
                                                                                                                                               tCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCAGCTTGCTGACTACCAGCAGGCGCTGGACGTCCAGCAAACGCGCGCGATCCAGTATAACCAGGCGATTGCTGCGCTTAATCGTGCCAAAGAACTGTGCCATCTg        <  2:192044/148‑1 (MQ=255)
                                                                                                                                                    cGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCAGCTTGCTGACTACCAGCAGGCGCTGGACGTCCAGCAAACGCGCGCGATCCAGTATAACCAGGCGATTGCTGCGCTTAATCGTGCCAAAGAACTGTGCCATCTGCCGGAc  <  3:656932/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CACCTGAACCTGGTGCAAACCGCACTGCGTCAGCAGGAGAAGATCGAACGCTACGAAGCGGATCTCGATGAGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCAGCTTGCTGACTACCAGCAGGCGCTGGACGTCCAGCAAACGCGCGCGATCCAGTATAACCAGGCGATTGCTGCGCTTAATCGTGCCAAAGAACTGTGCCATCTGCCGGAC  >  NC_000913/977331‑977627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: