| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 83,587 | A→C | 10.2% | intergenic (‑58/+35) | leuA ← / ← leuL | 2‑isopropylmalate synthase/leu operon leader peptide |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 83,587 | 0 | A | C | 10.2% | 386.5 / 36.7 | 128 | intergenic (‑58/+35) | leuA/leuL | 2‑isopropylmalate synthase/leu operon leader peptide |
| Reads supporting (aligned to +/- strand): ref base A (53/62); new base C (9/4); total (62/66) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.47e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.20e-01 | |||||||||||
Only 100 of 132 total aligned reads displayed.
TTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATA > NC_000913/83439‑83734 | ttGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCa > 1:83926/1‑148 (MQ=255) tGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCaa < 1:236027/148‑1 (MQ=255) aGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGgc > 3:339984/1‑149 (MQ=255) ttttCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGgcgc > 2:751734/1‑148 (MQ=255) ttctttcACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCgg < 1:751734/148‑1 (MQ=255) tctttcACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCCATTGCGCggg < 1:406515/148‑1 (MQ=255) ttcACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGttt > 1:966294/1‑148 (MQ=255) tcACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGttttt > 3:881767/1‑149 (MQ=255) cacaCTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGttttt > 2:381981/1‑148 (MQ=255) cacaCTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGttttt < 2:244792/148‑1 (MQ=255) cacTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTtttg < 1:443107/148‑1 (MQ=255) acTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGgc > 3:822006/1‑135 (MQ=255) acTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGgc < 4:822006/135‑1 (MQ=255) acTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTtttgtt > 3:326026/1‑149 (MQ=255) acTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTtttgt < 1:121705/148‑1 (MQ=255) cTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTtttgttt < 3:377842/149‑1 (MQ=255) cctGCCTGTAACGCCTTTTCCCCGGCGCGCAATTTGGGATCGAAAATAATGACTTGGTGGGCCATTGTTTGGGTTCTTGTTTCTTTTAGAGCGCCCCGCTTCCGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTg < 1:147822/146‑1 (MQ=255) tGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCg > 1:614821/1‑148 (MQ=255) tGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGt < 4:838506/149‑1 (MQ=255) cTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCCATTGCGCGGGt < 3:556175/129‑1 (MQ=255) cTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCCATTGCGCGGGt > 4:556175/1‑129 (MQ=255) tAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGc < 3:77868/149‑1 (MQ=255) aaCGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCt > 3:615694/1‑149 (MQ=255) ccTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTaa > 2:928355/1‑148 (MQ=255) ccTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAAt < 3:132384/149‑1 (MQ=255) ttCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTg < 4:86564/149‑1 (MQ=255) aCCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGAt > 4:124838/1‑149 (MQ=255) tCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGATGCCg < 3:470722/149‑1 (MQ=255) aTGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGATGCCGCTCACt > 1:479788/1‑148 (MQ=255) tgGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGATGCCGCTCACTCGTc > 3:72550/1‑149 (MQ=255) tgGTATCGAAAATAATGACTCGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGGTTCGGGCATAAAAAAACCCGCGCAAGGGCGCAGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGATACCGCTCACTCGt < 2:281071/148‑1 (MQ=255) tCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAGAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACcg > 4:1188/1‑149 (MQ=255) gAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAAt < 4:145428/84‑1 (MQ=255) aaaTAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCgcgc > 2:889796/1‑148 (MQ=255) aataatGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCa > 1:931134/1‑148 (MQ=255) ataatGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCaa < 2:417339/148‑1 (MQ=255) atGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAaagaa < 4:881767/149‑1 (MQ=255) atGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAaagaa < 4:326026/149‑1 (MQ=255) ttGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGGGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTTGATGCGTGGCGGGCTTAATGCGGGAGGCGGCTAACTCGCTTACGGGGCAAAGAAGATg > 3:666943/1‑149 (MQ=255) tGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGG‑TTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCg > 1:411235/1‑148 (MQ=255) cTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGtt < 3:453492/149‑1 (MQ=255) cTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGtt < 3:363981/149‑1 (MQ=255) ggCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGttt > 2:593302/1‑148 (MQ=255) tCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGa > 4:718807/1‑107 (MQ=255) tCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGa < 3:718807/107‑1 (MQ=255) tCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtagta > 4:248314/1‑149 (MQ=255) tCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtagt < 2:580928/148‑1 (MQ=255) tCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtagt < 2:930424/148‑1 (MQ=255) tCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGT‑ACTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtagta > 3:815167/1‑149 (MQ=255) cATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGttt < 2:955972/144‑1 (MQ=255) cATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGACTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGttt > 1:955972/1‑144 (MQ=255) tGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtagtagt < 1:833523/148‑1 (MQ=255) gTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtagtagtagt < 3:124838/149‑1 (MQ=255) gggTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGa < 1:965038/148‑1 (MQ=255) ggTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGAcc > 3:233450/1‑149 (MQ=255) ccTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGa > 2:598506/1‑148 (MQ=255) ccTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGa > 2:350099/1‑148 (MQ=255) cTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGAt > 1:925389/1‑148 (MQ=255) ttGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGATGCCg < 3:448975/97‑1 (MQ=255) ttGGCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGATGCCg > 4:448975/1‑97 (MQ=255) tGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATaac < 4:823892/149‑2 (MQ=255) tGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATaa < 1:414507/148‑1 (MQ=255) tGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATaa < 1:889796/148‑1 (MQ=255) tGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATaa > 2:204984/1‑148 (MQ=255) gTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATaaa < 1:950605/148‑1 (MQ=255) tctcTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGtt > 1:631942/1‑126 (MQ=255) tcgctTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGtt < 2:631942/123‑1 (MQ=255) tctTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGa > 3:261578/1‑149 (MQ=255) ttttAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAAc > 3:478353/1‑149 (MQ=255) ttttAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAAc > 3:343633/1‑149 (MQ=255) gcgcCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGAtgtg < 1:577098/148‑1 (MQ=255) gcgcCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGAtgtg < 1:204984/148‑1 (MQ=255) gcCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAg < 2:966294/148‑1 (MQ=255) ccTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGt < 1:788016/148‑1 (MQ=255) ccTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTc > 4:861578/1‑149 (MQ=255) tCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGa > 4:470827/1‑144 (MQ=255) tCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGa < 3:470827/144‑1 (MQ=255) tCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCAt > 4:404501/1‑149 (MQ=255) cGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCa < 2:694386/147‑1 (MQ=255) gCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCAtt < 2:479788/148‑1 (MQ=255) ttCGGGCATAAAAAAACCCGCGCAATGGCGCGGGCTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGGCATTaa > 2:897743/1‑148 (MQ=255) ggCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAg < 3:557470/149‑1 (MQ=255) aTAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTc > 3:536477/1‑149 (MQ=255) tAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTcc > 3:432869/1‑149 (MQ=255) ataaaaaaCCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAATAAGATGCGTTTAATAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTcc < 4:700346/147‑1 (MQ=255) aaaaaaaCCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCa > 3:896236/1‑149 (MQ=255) aaaaaaCCCGCGCCATTGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAg > 4:150583/1‑149 (MQ=255) aaaaaCCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGa < 4:615694/149‑1 (MQ=255) aaCCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATg < 2:570862/148‑1 (MQ=255) aCCCGCGCCATTGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGa < 3:601141/125‑1 (MQ=255) aCCCGCGCCATTGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGa > 4:601141/1‑125 (MQ=255) aCCCGCGCCATTGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGa > 1:688574/1‑148 (MQ=255) aCCCGCGCCATTGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCACAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAAt < 2:892632/135‑1 (MQ=255) aCCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGa < 1:714887/148‑1 (MQ=255) cccGCGCCATTGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGaa > 2:549162/1‑148 (MQ=255) cccGCGCCATTGCGCGGGTTTTTGTTTTGCCTGCGT‑GCTGGCTTAATCCTGAATCCCGCTCACTGGTCTACCGCGCAAAGAAGATGCGTTAGGTAGTAGTAGACCGATAAAGCGAACGATGGGAGTCATTAAATAAGTTCCTGATGaag > 3:821826/1‑148 (MQ=255) cccGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAAt < 4:261578/149‑1 (MQ=255) cgcAACGGGGCGGGTGTTTTGTTTGACTGCGT‑GCGGGCGTAATGCTGGATGCCGCTCACGCGTCCACCGCGCAAAGAAGATGCGAGTAGTAGGAGGAGACCGATAAAGCGAACGAGGGGAGTCATTAAATCAGCTCCAGATGAATGCg < 2:561745/148‑1 (MQ=255) cAATGGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGata < 4:343633/149‑1 (MQ=255) cAATGGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGata < 4:170880/149‑1 (MQ=255) | TTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGT‑GCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATA > NC_000913/83439‑83734 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |