BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,597,272	T→A	12.9%	pseudogene (716/3861 nt)	yneO ←	pseudogene, AidA homolog

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,597,272	0	T	A	12.9%	323.5 / 32.2	108	pseudogene (716/3861 nt)	yneO	pseudogene, AidA homolog
Reads supporting (aligned to +/- strand): ref base T (53/41); new base A (8/6); total (61/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.42e-01

Only 100 of 111 total aligned reads displayed.

AACGCCCACCAATGAATATTCTTTGTTATTAATCAGTCCATTATTGGAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTTTATTAGCCACTCGCCACCCTTTTCAACGACAACCTGGCC  >  NC_000913/1597124‑1597420

aaCGCCCACCAATGAATATTCTTTGTTATTAATCAGTCCATTATTGGAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCAt                                                                                                                                                      <  3:669222/149‑1 (MQ=255)

 aCGCCCACCAATGAATATTCTTTGTTATTAATCAGTCCATTATTGGAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCAtt                                                                                                                                                     <  3:68321/149‑1 (MQ=255)

         caATGAATATTCTTTGTTATTAATCAGTCCATTATTGGAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGa                                                                                                                                             <  3:281718/149‑1 (MQ=255)

          aaTGAATATTCTTTGTTATTAATCAGTCCATTATTGGAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGAt                                                                                                                                            >  3:870728/1‑149 (MQ=255)

           aTGAATATTCTTTGTTATTAATCAGTCCATTATTGGAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGAt                                                                                                                                            >  1:471495/1‑148 (MQ=255)

            tGAATATTCTTTGTTATTAATCAGTCCATTATTGGAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGTCAATGCCACCGGTa                                                                                                                                           <  1:799017/148‑1 (MQ=255)

            tGAATATTCTTTGTTATTAATCAGTCCATTATTGGAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATa                                                                                                                                           >  1:521763/1‑148 (MQ=255)

             gAATATTCTTTGTTATTAATCAGTCCATTATTGGAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAAt                                                                                                                                         >  3:53207/1‑149 (MQ=255)

             gAATATTCTTTGTTATTAATCAGTCCATTATTGGAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAAt                                                                                                                                         <  3:539799/149‑1 (MQ=255)

               atatTCTTTGTTATTAATCAGTCCATTATTGGAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCAATGCCACCGGTatcg                                                                                                                                        <  2:571963/148‑4 (MQ=255)

                  ttCTTTGTTATTAATCAGTCCATTATTGGAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTa                                                                                                                                     <  1:337140/148‑1 (MQ=255)

                  ttCTTTGTTATTAATCAGTCCATTATTGGAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTAt                                                                                                                                    >  3:410615/1‑149 (MQ=255)

                     tttGTTATTAATCAGTCCATTATTGGAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTAtttt                                                                                                                                 >  4:221626/1‑149 (MQ=255)

                     tttGTTATTAATCAGTCCATTATTGGAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCAATGCCAc                                                                                                                                                <  3:66993/134‑1 (MQ=255)

                     tttGTTATTAATCAGTCCATTATTGGAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCAATGCCAc                                                                                                                                                >  4:66993/1‑134 (MQ=255)

                      ttGTTATTAATCAGTCCATTATTGGAAATATTGACTGTGTCAATACCGAGATATACATTATAGAGTCTGCGTACCTTGATGACAGCGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCGACCGAGAGTCGTGtttt                                                                                                                                 >  2:577465/1‑148 (MQ=255)

                           attaATCAGTCCATTATTGGAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGcc                                                                                                                            >  1:255004/1‑148 (MQ=255)

                               aTCAGTCCATTATTGGAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTaa                                                                                                                        >  2:190333/1‑148 (MQ=255)

                               aTCAGTCCATTATTGGAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACAGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTaac                                                                                                                       >  3:233357/1‑149 (MQ=255)

                                tCAGTCCATTATTGGAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCGCATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTaac                                                                                                                       >  2:870291/1‑148 (MQ=255)

                                tCAGTCCATTATTGGAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTaacc                                                                                                                      >  4:649649/1‑149 (MQ=255)

                                   gTCCATTATTGGAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTaaccaac                                                                                                                   >  4:495492/1‑149 (MQ=255)

                                   gTCCATTATTGGAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTaaccaac                                                                                                                   <  3:657680/149‑1 (MQ=255)

                                    tCCATTATTGGAAATATTGACTGTGCCATTACCGAAATATCCTTTATAGAGTCGGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGCCATTGCCACCGATAATCGTATTTTCAGCCGTaaccaacc                                                                                                                  >  4:879769/1‑149 (MQ=255)

                                    tCCATTATTGGAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTaaccaacc                                                                                                                  >  3:603888/1‑149 (MQ=255)

                                    tCCATTATTGGAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTaaccaacc                                                                                                                  >  3:748945/1‑149 (MQ=255)

                                             ggAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTcg                                                                                                          <  2:285333/148‑1 (MQ=255)

                                              gAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTcgcg                                                                                                        >  4:223874/1‑149 (MQ=255)

                                                 atatTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAAt                                                                                                     <  3:618190/149‑1 (MQ=255)

                                                  tatTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATa                                                                                                    <  4:603888/149‑1 (MQ=255)

                                                   atTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAg                                                                                                   >  3:581242/1‑149 (MQ=255)

                                                     tGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGt                                                                                                  <  1:694834/148‑1 (MQ=255)

                                                          gtgCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGTCAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCt                                                                                             <  1:626524/148‑1 (MQ=255)

                                                            gCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTcc                                                                                           <  2:521763/148‑1 (MQ=255)

                                                                 tACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGtt                                                                                     >  4:816243/1‑149 (MQ=255)

                                                                 tACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGt                                                                                      >  1:302156/1‑148 (MQ=255)

                                                                     gAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCtt                                                                                 <  4:535679/149‑1 (MQ=255)

                                                                     gAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCtt                                                                                 >  4:555958/1‑149 (MQ=255)

                                                                     gAAATATCCATTAGAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTGTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCt                                                                                  <  1:495608/148‑1 (MQ=255)

                                                                            ccATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTAc                                                                           >  2:740763/1‑148 (MQ=255)

                                                                            ccATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTAc                                                                           >  2:662989/1‑148 (MQ=255)

                                                                             cATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCa                                                                         >  3:159359/1‑149 (MQ=255)

                                                                             cATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCa                                                                         >  3:5089/1‑149 (MQ=255)

                                                                             cATTATAGAGTCTGCGTACCGTGATGACAAAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAGTCGTATTTTCAGCCGTAACCAACCGCCCCTCGCGCATAGTCGCCTCCCCAGTTCCTTGATTACCa                                                                         >  3:775684/1‑149 (MQ=255)

                                                                              aTTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCaa                                                                        >  4:138668/1‑149 (MQ=255)

                                                                                  taGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTg                                                                    <  3:495492/149‑1 (MQ=255)

                                                                                   agagTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCCCCCCAGTTCCTTGATTACCAATTTg                                                                    >  2:951801/1‑148 (MQ=255)

                                                                                    gagTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGa                                                                   >  2:751136/1‑148 (MQ=255)

                                                                                     agTCTGCGTACCGTGATGACAGAGTCTTGATTCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGaa                                                                  <  2:302156/148‑1 (MQ=255)

                                                                                     agTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGaaa                                                                 <  4:53207/149‑1 (MQ=255)

                                                                                     agTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGaa                                                                  <  1:190333/148‑1 (MQ=255)

                                                                                     agTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGaa                                                                  <  1:487461/148‑1 (MQ=255)

                                                                                     agTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGaa                                                                  <  1:458539/148‑1 (MQ=255)

                                                                                         tGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCa                                                             <  3:698985/149‑1 (MQ=255)

                                                                                          gCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCa                                                             <  1:751136/148‑1 (MQ=255)

                                                                                            gTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAAtt                                                          <  4:773501/149‑1 (MQ=255)

                                                                                            gTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAAt                                                           <  1:951801/148‑1 (MQ=255)

                                                                                              gccGGGATGACAGTGTCTCCATCTTGTACATTCAGGGCTCCAATACAGGTGGAATTGCCGCCTAAAATCGTATTTTCAGCGGGAACTACACCACCATCGCGAATAGTCGCCTCCCCAGCTCCTTGATTACCACTTTGAAATTccagtg                                                         <  2:700975/147‑6 (MQ=255)

                                                                                              aCCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTg                                                         <  2:255004/148‑1 (MQ=255)

                                                                                               ccGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAg                                                       <  3:221626/149‑1 (MQ=255)

                                                                                                  tgatgaCAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGATGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGaa                                                    <  3:816243/149‑1 (MQ=255)

                                                                                                     tgaCAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATc                                                  >  1:27313/1‑148 (MQ=255)

                                                                                                          gagTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCattat                                             >  1:548510/1‑148 (MQ=255)

                                                                                                           agTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCattatt                                            >  2:338907/1‑148 (MQ=255)

                                                                                                            gTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTAtttt                                          <  4:870728/149‑1 (MQ=255)

                                                                                                             tCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTAtttt                                          <  1:480434/148‑1 (MQ=255)

                                                                                                              cTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGAt                                                                              <  2:850881/111‑1 (MQ=255)

                                                                                                              cTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGAt                                                                              >  1:850881/1‑111 (MQ=255)

                                                                                                               ttGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTtta                                       <  3:248483/149‑1 (MQ=255)

                                                                                                               ttGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTtta                                       >  4:762510/1‑149 (MQ=255)

                                                                                                               ttGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAAACAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTtta                                       <  4:5089/149‑1 (MQ=255)

                                                                                                                tGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTTACTTGAGGAATCATTATTTTtta                                       >  1:97706/1‑148 (MQ=255)

                                                                                                                tGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTtta                                       >  2:443993/1‑148 (MQ=255)

                                                                                                                 gATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTttatt                                     <  4:410615/149‑1 (MQ=255)

                                                                                                                  aTCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTttatta                                    >  4:222389/1‑149 (MQ=255)

                                                                                                                  aTCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTttatt                                     >  1:907255/1‑148 (MQ=255)

                                                                                                                    ccTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTTTATTAg                                   >  1:209960/1‑148 (MQ=255)

                                                                                                                    ccTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTTTATTAGc                                  <  3:649649/149‑1 (MQ=255)

                                                                                                                      tGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTTTATTAGCCa                                >  4:885465/1‑149 (MQ=255)

                                                                                                                             tCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAAtt                                                               >  1:435692/1‑111 (MQ=255)

                                                                                                                             tCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAAtt                                                               <  2:435692/111‑1 (MQ=255)

                                                                                                                             tCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTTTATTAGCCACTCGcc                          >  2:512865/1‑148 (MQ=255)

                                                                                                                                  gTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCATCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTTTATTAg                                   <  3:890656/134‑1 (MQ=255)

                                                                                                                                    tCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTTTATTAGCCACTCGCCACCCttt                   >  2:104605/1‑148 (MQ=255)

                                                                                                                                    tCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTTTATTAGCCACTCGCCACCCttt                   >  2:346160/1‑148 (MQ=255)

                                                                                                                                      gattatcGGTGGCAATGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTTTATTAGCCACTCGCCACCCTTTTCa                >  3:465090/7‑149 (MQ=255)

                                                                                                                                      gattatcGGTGGCAATGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTTTATTAGCCACTCGCCACCCTTTTCa                >  4:490306/7‑149 (MQ=255)

                                                                                                                                       gATACCGGTGGCATTGCCACCGATAATCGTCTTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCGGCAGTTCCTTGAt                                                                              <  2:248918/86‑1 (MQ=255)

                                                                                                                                       gATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGAt                                                                              >  1:248918/1‑86 (MQ=255)

                                                                                                                                       attatcGGTGGCAATGCCACCGCTAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTTTATTAGCCACTCGCCACCCTTTTCa                >  1:154760/6‑148 (MQ=255)

                                                                                                                                       attatcGGTGGCAATGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTTTATTAGCCACTCGCCACCCTTTTCa                >  1:677680/6‑148 (MQ=255)

                                                                                                                                        ttatcGGTGGCAATGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTTTATTAGCCACTCGCCACCCtt                    >  2:470540/5‑143 (MQ=255)

                                                                                                                                        ttatcGGTGGCAATGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTTTATTAGCCACTCGCCACCCTTTTCAAc              >  4:683304/5‑149 (MQ=255)

                                                                                                                                          atcGGTGGCAATGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTTTATTAGCCACTCGCCACCCTTTTCAACGa            <  4:465090/147‑1 (MQ=255)

                                                                                                                                               tGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTTTATTAGCCACTCGCCACCCTTTTCAACGACAAcc       >  3:383421/1‑149 (MQ=255)

                                                                                                                                               tGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTTTATTAGCCACTCGCCACCCTTTTCAACGACAAc        >  1:862244/1‑148 (MQ=255)

                                                                                                                                                ggCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTTTATTAGCCACTCGCCACCCTTTTCAACGACAAcc       <  2:548510/148‑1 (MQ=255)

                                                                                                                                                   aTTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTTTATTAGCCACTCGCCACCCTTTTCAACGAGAACCTGGc   <  3:879769/149‑1 (MQ=255)

                                                                                                                                                   aTTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTTTATTAGCCACTCGCCACCCTTTTCAACGACAACCTGGc   >  3:892043/1‑149 (MQ=255)

                                                                                                                                                    ttGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTTTATTAGCCACTCGCCACCCTTTTCAACGACAACCTGGcc  <  3:223874/149‑1 (MQ=255)

AACGCCCACCAATGAATATTCTTTGTTATTAATCAGTCCATTATTGGAAATATTGACTGTGCCATTACCGAAATATCCATTATAGAGTCTGCGTACCGTGATGACAGAGTCTTGATCCTGCACATTCAGGGTTCCGATACCGGTGGCATTGCCACCGATAATCGTATTTTCAGCCGTAACCAACCCCCCCTCGCGAATAGTCGCCTCCCCAGTTCCTTGATTACCAATTTGAAATTCAATTGAGGAATCATTATTTTTTATTAGCCACTCGCCACCCTTTTCAACGACAACCTGGCC  >  NC_000913/1597124‑1597420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`